期刊论文

Silencing of ezrin gene inhibits proliferation and invasion of human prostate cancer PC-3 cells

中文

细胞与分子免疫学杂志

1007-8738

2016

32

6

821-824

10.13423/j.cnki.cjcmi.007797

湖南省科技厅基金（2015JC3088） 衡阳市科技局基金（2010kj46）

本校为第一机构

目的观察埃兹蛋白（ezrin）在前列腺癌组织中的表达,探讨ezrin基因沉默对人前列腺癌PC-3细胞增殖、侵袭的影响及相关机制。方法收集前列腺癌和良性前列腺增生患者的标本各20例,采用荧光实时定量PCR检测ezrin mRNA水平、Western blot法检测ezrin蛋白水平。培养PC-3细胞,分别用培养液、Scramble siRNA、ezrin siRNA处理48 h;MTT法检测细胞增殖情况,TranswellTM侵袭实验检测细胞侵袭能力,荧光实时定量PCR、Western blot法检测上皮钙黏素、神经钙黏素的mRNA和蛋白水平。结果与良性前列腺增生患者的组织相比,前列腺癌组织中ezrin mRNA、蛋白表达水平均明显增加。与Scramble siRNA处理的PC-3细胞相比,...

Objective To observe the expression of ezrin in prostate cancer tissues and explore the effect of ezrin gene silencing on the proliferation and invasion of human prostate cancer PC-3 cells and involved mechanisms. Methods The paraffin-embedded specimens of prostate cancer (n=20) and benign prostatic hyperplasia (n=20) were collected. Real-time quantitative PCR and Western blotting were used to detect the expression of ezrin mRNA and protein in prostate tissues, respectively. After culturing, human prostate cancer PC-3 cells were treated with culture solution, Scramble siRNA and ezrin siRNA for...