摘要：
The purpose of this study was to investigate the humoral and cellular immune responses stimulated by multiple antigen peptides (MAPs) containing the mimic epitopes of Mycoplasma genitalium adhesion protein (MgPa). Three MAPs containing the mimic epitopes of MgPa were synthesized on a branched polylysine matrix. After purification and characterization, these MAPs were used to immunize BALB/c mice. The immunoglobulin G (IgG) antibody and the subtype of IgG antibody in the serum of the immunized mice were detected by indirect ELISA (enzyme-linked immunosorbent assay). The proliferation of the spleen lymphocyte was detected using the MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide) assay. The gamma interferon (IFN-γ) and interleukin-4 (IL-4) levels in the cultured supernatant of spleen lymphocytes were measured by ELISA. The 3 different MAPs were prepared with high purity. Levels of IgG, IgG1, and IgG2a antibodies were elevated in the mice serum immunized by all 3 MAPs. The major antibody isotype was IgG2a. Importantly, mice immunized with a mixture of the 3 MAPs produced significantly more antibodies than those immunized with a single MAP (p < 0.05). Moreover, these MAPs could stimulate the proliferation of spleen lymphocytes of immunized mice and induce the production of IFN-γ and IL-4. The IFN-γ and IL-4 levels stimulated by the mixed MAPs were significantly higher than those stimulated by a single MAP (p < 0.01). The 3 different MAPs could induce strong cellular and humoral immune responses. The immunoreactivity of the mixed MAPs was stronger than that of the single MAP.
期刊：
CANADIAN JOURNAL OF MICROBIOLOGY,2012年58(5):644-652 ISSN：0008-4166
通讯作者：
Wang, Shiping
作者机构：
[Wang, Shiping; Zhu, Cuiming] Cent S Univ, Xiangya Sch Med, Changsha 410078, Hunan, Peoples R China.;[Wu, Yimou; Xiao, Jinhong; Yu, Minjun; You, Xiaoxing; Zeng, Yanhua; Chen, Sufang] Univ S China, Dept Microbiol & Immunol, Hengyang 421001, Peoples R China.
通讯机构：
[Wang, Shiping] C;Cent S Univ, Xiangya Sch Med, Changsha 410078, Hunan, Peoples R China.
关键词：
Mycoplasma pneumoniae;P1 adhesin protein carboxy terminal region;DNA vaccine;immune response;Mycoplasma pneumoniae;région carboxy terminale de l’adhésine P1;vaccin à ADN;réponse immune
摘要：
Mycoplasma pneumoniae is an important causative agent of atypical pneumonia. This study was to determine the ability of a DNA expression vector, which encodes the carboxy terminal region of the M. pneumoniae P1 protein (P1C), to induce humoral and cellular immune responses and to protect against M. pneumoniae infection in BALB/c mice. Mice were immunized with pcDNA3.1/P1C by either intramuscular injection (i.m.) or intranasal inoculation (i.n.). Our results showed that p1c DNA immunization generates detectable antibodies specific to M. pneumoniae, and elicits high levels of IgG1, IgG2a, and IgG2b isotypes (P < 0.01). The levels of IFN-γ and IL-4 in spleen cells of the immunized mice were significantly elevated by immunization via both the i.m. and i.n. methods. Moreover, p1c DNA-immunized mice exhibited detectable protection against M. pneumoniae infection. The lung tissue inflammation was relieved and the histopathologic score (HPS) of pcDNA3.1/P1C-immunized mice was significantly decreased than those in phosphate-buffed saline (PBS) or vaccine-vector-immunized mice (P < 0.01), whereas there were no significant differences in HPS between i.m. and i.n. vaccination (P > 0.05). Our results suggest that pcDNA3.1/P1C could be useful for developing a vaccine against M. pneumoniae infection.
通讯机构：
[Wu, Yimou] U;Univ S China, Pathogen Biol Inst, Dept Microbiol & Immunol, Hengyang 421001, Peoples R China.
关键词：
Mycoplasma pneumoniae;P1 adhesin protein carboxy terminal region;the B subunit of Escherichia coliheat-labile enterotoxin;DNA vaccine;Mycoplasma pneumoniae;région carboxyterminale de l’adhésine P1;sous-unité B de l’entérotoxine thermolabile d’Escherichia coli;vaccin à ADN
摘要：
In the present study, we investigated the immunomodulatory responses of a DNA vaccine constructed by fusing Mycoplasma pneumoniae P1 protein carboxy terminal region (P1C) with the Escherichia coli heat-labile toxin B subunit (LTB). BALB/c mice were immunized by intranasal inoculation with control DNAs, the P1C DNA vaccine or the LTB-P1C fusion DNA vaccine. Levels of the anti-M. pneumoniae antibodies and levels of interferon-gamma and IL-4 in mice were increased significantly upon inoculation of the LTB-P1C fusion DNA vaccine when compared with the inoculation with P1C DNA vaccine. The LTB-P1C fusion DNA vaccine efficiently enhanced the M. pneumoniae-specific IgA and IgG levels. The IgG2a/IgG1 ratio was significantly higher in bronchoalveolar lavages fluid and sera from mice fusion with LTB and P1C than mice receiving P1C alone. When the mice were challenged intranasally with 10(7) CFU M. pneumoniae strain (M129), the LTB-P1C fusion DNA vaccine conferred significantly better protection than P1C DNA vaccine (P < 0.05), as suggested by the results, such as less inflammation, lower histopathological score values, lower detectable number of M. pneumoniae strain, and lower mortality of challenging from 5 x 10(8) CFU M. pneumoniae. These results indicated that the LTB-P1C fusion DNA vaccine efficiently improved protective efficacy against M. pneumoniae infection and effectively attenuated development of M. pneumoniae in mice.
期刊：
CANADIAN JOURNAL OF MICROBIOLOGY,2012年58(7):898-908 ISSN：0008-4166
通讯作者：
Wu, Yimou
作者机构：
[Liu, Yan; Wu, Yimou; Liu, Liangzhuan; Zhu, Cuiming; You, Xiaoxing; Zeng, Yanhua] Univ S China, Inst Pathogen Biol, Hengyang 421001, Peoples R China.;[He, Jun] Univ S China, Affiliated Nanhua Hosp, Hengyang 421000, Peoples R China.
通讯机构：
[Wu, Yimou] U;Univ S China, Inst Pathogen Biol, Hengyang 421001, Peoples R China.
关键词：
phage display peptide library;mimic epitope;Mycoplasma genitalium;adhesion protein;banque d’exposition de peptides sur phage;mimes fonctionnels d’épitopes;Mycoplasma genitalium;protéine d’adhésion
摘要：
Mycoplasma genitalium adhesion protein (MgPa) is the major adhesion protein of M. genitalium, and its Cterminal domain (amino acid 1075-1444) is the most immunogenic region. However, the exact epitopes of the adhesion protein of M. genitalium are still unclear. We used the purified polyclonal antibody against the recombinant adhesion protein to screen the mimic epitopes of MgPa using a random 12-peptide phage display library. Immunoscreening via the phage display peptide library revealed that 3 motifs (P-S-A-A/V-X-R-F/W-E/S-L-S-P, A-K-I/L-T/Q-X-T-L-X-L, and K-S-L-S-R-X-DX- I) may represent 3 different mimotopes of MgPa. Results of bioinformatics analysis by MIMOX demonstrated that the key consensus amino acid residues in the aligned mimotopes may be S, A, and F for cluster 1; A, K, I, T, and L for cluster 2; and K, S, L, R, D, and I for cluster 3. Three representative phages could recognize sera from M. genitalium-positive patients to varying degrees, whereas they could not recognize the sera from Mycoplasma pneumoniae-positive patients or the sera from healthy people. These findings will help to clarify the mimic epitopes of MgPa to facilitate diagnosis of the antigen and to understand the antigenic structure of MgPa.
作者：
Li Chun;Wu Yimou;Zhu Cuiming;Zhong Lili;Lü Jianhua
作者机构：
[Li Chun; Wu Yimou; Zhu Cuiming] Pathogenic Biology Institute, School of medicine,University of South China, Hengyang 421001, China;[Zhong Lili] Pediatrics, Hunan Provincial People's Hospital,Changsha 410000, China;[Lü Jianhua] Pediatrics, The First Hospital of Nanhua University,Hengyang 421001, China
会议名称：
中华医学会第七次全国中青年检验医学学术会议
会议时间：
2012-5-3
会议地点：
南京
会议主办单位：
中华医学会
会议论文集名称：
中华医学会第七次全国中青年检验医学学术会议论文集
摘要：
<正>Background & objectives Mycoplasma pneumoniae is known to be a major cause of respiratory tract infections and pneumonia in children.Macrolides are generally considered as the treatment of choice in children.A specific diagnosis is important to institute the appropriate treatment.The aim of this study was to detect mycoplasma pneumoniae from clinical samples
作者：
Yang Fan;SuFang Chen;Zhang Qin;CuiMing Zhu;Chen Jie;...
期刊：
World Health Digest,2012年(51):6-8
作者机构：
Department ofMicrobiology and Immunology,Xiang Nan college of Medicine Department of basic medicine,423000;Institute of Pathogenic Biology,Nanhua University,Hengyang,Hunan,421001
关键词：
human papillomavirus type 18(HPV18);E2;IL-12;vaccine;human papillomavirus type 18(HPV18);E2;IL-12;vaccine
摘要：
Objective:To study the effectiveness of human papillomavirus type 18 E2 gene (HPV18-E2) vaccine and interleukin-12(IL-12) on their immune effects. Methods:Thirty-five Balb/c female mice aged 6-8-week-old were randomly divided into five groups (each n =7).The mice were given instramuscular injection of DNA vaccines at 2-week four 4 times(100mg/time).The tails were cut to draw blood at the 0,2,4,6 weeks,and then the mice were executed by eyeball blood letting at 8 weeks.ELISA was used for the quantitative detection of the specific lgG antibody in the srea of Balb/c mice and the cytokine IFN-γ and IL-4 in mice MTT assay.Results: after 8 weeks immunization, antibody IgA in E2 vaccine group andE2 +IL-12 vaccine group was statistically increased as compared with that of control group, and the difference was statistically significant (P<0.01). IFN-γand IL-4 levels of mice spleen lymphocyte culture supernatant inE2 group and E2 + IL-12 were significantly higher than other control groups (P<0.01). the spleen lymphopoiesis activeness of E2 + IL-12 group was significant enhanced as compared with the E2 group, there were statistically significant differences (P<0.01) . Conclusions: E2 DNA vaccine combined with IL-12 immune mice can effectively induce cell-mediated immunity and humoral immune responses, their capabilities of inducing immune response might be more stronger than that of single one.
摘要：
As a highly conserved nuclear protein, Daxx plays an important role in transcriptional control, tumorigenesis, viral infection, and other processes. Daxx can be localized in promyelocytic leukemia nuclear bodies (PML-NBs), nucleoplasm, nucleoli, cytoplasm, and heterochromatln. The subcellular localization of Daxx can be changed by modification or by Interacting with other proteins. Under cellular stress, Daxx can interact with many kinds of molecules, and thus affect its downstream signaling pathway. The purpose of this review Is to discuss the subcellular localization of Daxx under different conditions and Its translocation between subcellular compartments.
