期刊论文

Yu, X.-P.

中文

中华肿瘤防治杂志

1673-5269

2010

17

21

1722-1726

10.16073/j.cnki.cjcpt.2010.21.005

(C2005044):湖南省卫生厅科研基金

本校为第一且通讯机构

目的:观察livin基因的siRNA对人胃癌细胞株BGC823生长与功能的影响。方法:构建针对livin基因siR-NA真核表达质粒pGPU6/GFP/Neo-livin,通过脂质体介导转染至BGC823细胞,半定量RT-PCR、蛋白质印迹法分别检测livin基因mRNA和蛋白表达,激酶活性检测试剂盒检测胱冬肽酶-3（Caspase-3）的活性,MTT法分别检测PBMC和BGC823细胞增殖活性,流式细胞仪检测细胞凋亡率,ELISA法检测混合细胞培养上清中TNF-α分泌量的变化。结果:转染48 h实验组细胞livin mRNA和蛋白表达水平明显受到抑制,表达抑制率分别为64.63%和62.80%,P

OBJECTIVE: To observe the effect of siRNA targeting livin gene on the growth and function of gastric cancer cell line BGC823. METHODS:siRNA expression vector pGPU6/GFP/Neo-livin was constructed and transfected into gastric cancer cell line BGC823. The expressions of livin mRNA and protein were detected by semi-quantitative and Western blot. The changes of Caspase-3 activity were assessed by a kinase activity test. Cell apoptosis was examined by flow cytometry. PBMC and BGC823 cell proliferation activities were examined by MTT. The co-culture se...