作者机构：
[余敏君; 朱翠明; 谭立志; 吴移谋] Department of Microbiology, Faculty of Medicine, Nanhua University, Hengyang 421001, China;[廖端芳] Department of Pharmacology, Faculty of Medicine, Nanhua University, Hengyang 421001, China;Nanhua University, Hengyang, China;[万艳平] Department of Microbiology, Faculty of Medicine, Nanhua University, Hengyang 421001, China, Nanhua University, Hengyang, China
通讯机构：
[Duan-fang Liao] D;Department of Pharmacology, Faculty of Medicine, Nanhua University, Hengyang
关键词：
Human Daxx;Ad12 E1B 55kD;Binding;Interaction
摘要：
Objective: To study the disruption of co- localization of human Daxx(hDaxx) with promyelocytic leukemia protein(PML) at the PML oncogenic domains (PODs) by the interaction of hDaxx with adenovirus(Ad) 12 E1B 55 Kilodalton Oncoprotein (Ad12 E1B 55kD). Methods: The direct binding reaction of hDaxx and Ad12 E1B 55kD was analyzed by coimmunoprecipitation and Western blotting in vivo or in vitro. The interaction of hDaxx with Ad12 E1B 55kD was studied using yeast two-hybrid assay. Results: hDaxx bounded directly to Ad12 E1B 55kD in vivo and in vitro. hDaxx interacted with full length Ad12 E1B 55kD. Conclusion: Transcriptional regulator hDaxx directly binds to and interacts with Ad12 E1B 55kD.
关键词：
active efflux system;ciprofloxacin;fluorescence
摘要：
Objective: To investigate the possible presence of an active efflux system in resistance to fluoroqninolones in Mycoplasma hominis. Methods: The resistant strains of M. hominis were selected from one hundred and three clinical strains of M. homlnls by broth microdilution method. The ac-cumulation of ciprofloxacin in M. hominis and the in-fluence of carbonyl cyanide m-chlorophenyl- hydrazone (CCCP) and reserpine were measured by a fluores-cence method. Results: Two resistant strains and two susceptible strains of M. hominis were selected in vitro. The accu-mulation of ciprofloxacin for resistant strains is lower than that of susceptible strains. CCCP and reserpine had different influence on clinical strains of M.hominis. Reserpine could dramatically increase the accumulation of ciprofloxacin, however CCCP had a little effect on it. Conclusion: These results suggest that the pres-ence of an active efflux system implicated in the fluoroouinolones-resistant in M. hominis.
