期刊论文

英文

中华性传播感染杂志：英文版

1608-6260

2003

3

1

58-61

Financially supported by a grant from the Education Committee of Hunan Province（No.00A009）；

本校为第一机构

Objective: To investigate the possible presence of an active efflux system in resistance to fluoroqninolones in Mycoplasma hominis. Methods: The resistant strains of M. hominis were selected from one hundred and three clinical strains of M. homlnls by broth microdilution method. The ac-cumulation of ciprofloxacin in M. hominis and the in-fluence of carbonyl cyanide m-chlorophenyl- hydrazone (CCCP) and reserpine were measured by a fluores-cence method. Results: Two resistant strains and two susceptible strains of M. hominis were selected in vitro. The accu-mulation of ciprofloxacin for resistan...

目的 探讨人型支原体对氟喹诺酮类药物的耐药性是否由于一种主动外排系统的存在所致.方法 采用肉汤微量稀释法从103株临床人型支原体中筛选出耐药菌株.用荧光法测定盐酸环丙沙星在人型支原体内的蓄积量及碳酰氰间氯苯腙(CCCP)和利血平产生的影响.结果 体外分离出两组人型支原体耐药菌株和两组敏感菌株.盐酸环丙沙星在耐药株中的蓄积量低于敏感株.CCCP和利血平对临床菌株产生的影响有差异.利血平能够显著增加盐酸环丙沙星的蓄积量,而CC C P在此方面作用不明显.结论 主动外排系统的存在可能是人型支原体对氟喹诺酮类药物耐药机制的一部分.