期刊论文

Zhu, C.

中文

细胞与分子免疫学杂志

1007-8738

2013

29

6

585-588,592

10.13423/j.cnki.cjcmi.006798

国家自然科学基金（81072418/H1005） 湖南省教育厅重点实验室资助项目（2012年）

本校为第一机构

目的研究肺炎支原体(Mp)P1C-IL-2融合基因疫苗经鼻饲免疫小鼠后的免疫应答水平和免疫保护作用,了解IL-2对P1C核酸疫苗的免疫佐剂效应。方法将构建的P1C-IL-2核酸疫苗鼻饲免疫BALB/c鼠,ELISA检测免疫小鼠血清IgG滴度、IgG亚类和支气管肺泡灌洗液中IgA及IFN-γ、IL-4的水平;建立小鼠Mp感染模型,观察Mp攻击后小鼠肺组织炎症情况和支气管肺泡灌洗液中Mp菌落数的变化。结果P1C-IL-2双基因疫苗组小鼠血清中的总IgG、IgGl、IgG2a亚类和支气管肺泡灌洗液中IFN-γ和IL-4水平均较P1C疫苗组小鼠显著增高(P0.05)。用Mp滴鼻感染免疫小鼠,第1、3、6天P1C-IL-2双基因融合疫苗组小鼠肺组织炎症病理评分显著高于P...

OBJECTIVE: To investigate the immune responses and immune protections of a DNA vaccine constructed by fusing Mycoplasma pneumoniae (M. pneumoniae) p1 gene car boxy terminal region (p1c gene) with interleukin-2 (IL-2) gene. METHODS: BALB/c mice were immunized by intranasal inoculation of P1C-IL-2 fusion DNA vaccine. Levels of serum IgG, IgG isotypes, BAL fluids IgA, IFN-gamma and IL-4 were detected by ELISA. We established the mouse models infected with M. pneumoniae, and then observed the histopathological changes in lungs and counted colonies in bronchoalveolar lavage fluid (BALF) after chall...