期刊：
Journal of Molecular Liquids,2020年303:112604 ISSN：0167-7322
通讯作者：
Li, Le
作者机构：
[Lu, Wei; Zhang, Peng; Liu, Ling; Chen, Tuo; Li, Le; Chen, Lei; Zhu, Bingyu; Li, Huiqin; Liu, Jinquan; Wang, Shengping; Sun, Lin; Yang, Huixian] Univ South China, Sch Publ Hlth, Hengyang 421001, Peoples R China.;[Lu, Wei; Li, Le; Dai, Zhongran] Univ South China, Hunan Prov Key Lab Green Dev Technol Extremely Lo, Hengyang 421001, Peoples R China.;[Lu, Wei; Liu, Ling; Chen, Tuo; Li, Le; Chen, Lei; Zhu, Bingyu; Liu, Jinquan; Sun, Lin] Univ South China, Key Lab Hlth Hazard Factors Inspect & Quarantine, Hengyang 421001, Peoples R China.;[Li, Le; Liu, Jinquan] Univ South China, Hunan Prov Key Lab Typ Environm Pollut & Hlth Haz, Hengyang 421001, Peoples R China.;[Cao, Chen] Univ South China, Sch Chem & Chem Engn, Hengyang 421001, Peoples R China.
通讯机构：
[Li, Le] U;Univ South China, Sch Publ Hlth, Hengyang 421001, Peoples R China.
关键词：
Hydrogel;Uranium;Adsorption
摘要：
In this work, a novel composite hydrogel (PCG) was constructed by mixing amidoxime polyacrylonitrile, chitosan and graphene oxide. The structure characterization of PCG was successfully investigated by FTIR, SEM. The effects of the pH, initial concentration of metal ion and contact time were conducted by a batch of experiments. PCG showed higher adsorption selectivity for U(VI) and its maximum adsorption capacity can reach up 247 mg/g. PCG can be completely separate from the aqueous solution and displayed excellent regeneration ability which would not cause secondary pollution to water bodies. The sorption kinetics of PCG for U(VI) followed pseudo-second order model well and Langmuir model were used to describe the equilibrium data well, which indicated that a monolayer adsorption occurred on PCG and the chemisorption was the main rate-limiting step. This work proved that PCG could be considered as the effective and promising material for removal of U(VI) from aqueous solution. (C) 2020 Elsevier B.V. All rights reserved.
摘要：
Type 2 diabetes mellitus (T2DM) is a chronic degenerative endocrine and metabolic disease with high mortality and morbidity, yet lacks effective therapeutics. We recently generated a novel fusion peptide INSR-IgG4Fc, Yiminsu (YMS), to facilitate the high-affinity binding and transportation of insulin. Thus, the aim of the present study was to determine whether the novel recombinant peptide, YMS, could contribute to restoring insulin sensitivity and glycaemic control in insulin resistance models and revealing its underlying mechanism. Palmitic acid (PA)-treated LO2 cells and high fat diet (HFD)-fed mice were treated with YMS. Therapeutic effects of YMS were measured using Western blotting, ELISA, qPCR, Histology and transmission electron microscopy. We observed that YMS treatment effectively improved insulin signaling in PA-treated LO2 cells and HFD-fed mice. Notably, YMS could significantly reduce serum levels of glucose, triglycerides, fatty acids and cholesterol without affecting the serum insulin levels. Moreover, our data demonstrated that YMS could restore glucose and lipid homeostasis via facilitating insulin transportation and reactivating PI3K/Akt signaling in both PA-treated cells and liver, gastrocnemius and brown fat of HFD-fed mice. Additionally, we noticed that the therapeutic effects of YMS was similar as rosiglitazone, a well-recognized insulin sensitizer. Our findings suggested that YMS is a potentially candidate for pharmacotherapy for metabolic disorders associated with insulin resistance, particularly in T2DM.
作者机构：
[让蔚清; 付由户; 刘洋; 翦耀文; 杨慧仙] Department of Center for Disease Control and Evaluation, University of South China, Hengyang, 421001, China;[熊文婧] Traditional Chinese Medicine Hospital of Hengyang, Hengyang, 421001, China
通讯机构：
[Rang, W.] D;Department of Center for Disease Control and Evaluation, University of South China, Hengyang, China
关键词：
乳腺癌;月经;病例-对照研究;女性;Meta 分析
摘要：
目的系统评价女性月经状况与乳腺癌发生的相关性。方法计算机检索 The Cochrane Library(2016年4期)、PubMed、EMbase、CNKI、WanFang Data、VIP和 ScienceDirect数据库,搜集月经状况与乳腺癌相关的病例-对照研究,检索时限均为建库至2016年6月1日。由2位研究者独立筛选文献、提取资料和评价纳入研究的偏倚风险后,采用 Stata 12.0软件进行 Meta分析。结果共纳入83个研究,包括48 811例患者和57 268例对照。Meta分析结果显示:乳腺癌与初潮年龄≤13岁[OR=1.17,95%CI(1.12,1.24)]和月经不规律[OR=1.98,95%CI(1.62,2.41)]具有相关性,但其与痛经史、月经周期≥30天、月经经期>7天的相关性无统计学意义。按照样本量进行亚组分析结果显示,样本量大小是痛经史的主要异质性来源,去除小样本研究后,相关性具有统计学意义[OR=1.47,95%CI(1.36,1.59)]。按照对照来源进行的亚组分析结果显示,乳腺癌与初潮年龄≤13岁、月经不规律与乳腺癌的相关性在对照来源是社区的研究中相关性具有统计学意义,但在对照来源是医院的研究中相关性无统计学意义。按照研究人群进行亚组分析结果显示,乳腺癌与初潮年龄≤13岁、月经不规律的相关性在中国人和外国人之间均有统计学意义。结论女性初潮年龄≤13岁、月经不规律、有痛经史可能是乳腺癌发病的危险因素。受纳入研究的数量和质量的限制,上述结论尚需要更多研究予以证实。
通讯机构：
[Wang, Yong-Sheng] U;Univ South China, Coll Publ Hlth, Hengyang 421001, Peoples R China.
关键词：
8-Hydroxy-2'-deoxyguanosine;Conformational switching;Fluorescence aptasensor;K(+)-stabilized G-quadruplex;N-methyl mesoporphyrin IX
摘要：
A sensitive, lable-free and low cost fluorescence aptasensor was developed for detecting 8-hydroxy-2'-deoxyguanosine (8-0HdG) by using 8-0HdG aptamer (Apt) as a recognition probe and N-methyl mesoporphyrin IX (NMM) as a reporter. The method is based on the conformational switching of a ICE-stabilized G-quadruplex to a 8-OHdG-stabilized one. NMM can selectively bind to K+-stabilized G-quadruplex instead of 8-OHdG-stabilized one. The addition of 8-0HdG in the solution of Apt - K+ ions leads to a sharp change in fluorescence intensity, which showed a good linear response toward 8-0HdG concentration ranging from 3.96 nM to 211 nM with a detection limit of 1.19 nM. The relative standard deviation and the recovery were 1.23-3.26% (n = 11) and 94.8-106.7%, respectively. The proposed aptasensor consists of only an aptamer probe and a specific dye NMM, avoiding the complex and expensive labeling procedure. Thus it is much cheaper and more applicable. (c) 2015 Elsevier B.V. All rights reserved.
作者：
Wang, J.;Zou, T.;Yang, H. X.;Gong, Y. Z.;Xie, X. J.;...
期刊：
Genetics and Molecular Research,2015年14(3):8819-8828 ISSN：1676-5680
通讯作者：
Liu, H. Y.
作者机构：
[Yang, H. X.; Wang, J.] Univ South China, Key Lab Atherosclerol Hunan Prov, Inst Cardiovasc Res, Hengyang, Hunan, Peoples R China.;[Yang, H. X.; Wang, J.] Univ South China, Sch Publ Hlth, Hengyang, Hunan, Peoples R China.;[Zou, T.] Univ South China, Affiliated Hosp 1, Dept Cardiovasc Med, Hengyang, Hunan, Peoples R China.;[Gong, Y. Z.; Xie, X. J.; Liao, D. F.] Hunan Univ Chinese Med, State Key Lab Chinese Med Powder & Med Innovat Hu, Div Stem Cell Regulat & Applicat, Changsha, Hunan, Peoples R China.;[Liu, H. Y.] Metammune LLC, Morrisville, NC 27560 USA.
通讯机构：
[Liu, H. Y.] M;Metammune LLC, Morrisville, NC 27560 USA.
摘要：
目的：优化五味子抗氧化物的提取条件；分析湖北产和辽宁产五味子提取物的 DPPH清除能力。方法以 DPPH自由基的清除能力为参考指标，采用单因素实验的方法，考察溶剂种类、乙醇浓度、料液比、提取时间、提取次数和提取方法等因素对五味子提取物抗氧化作用的影响。结果五味子抗氧化物的最优提取条件为：以60％的乙醇为提取溶剂，超声法提取，料液比为1∶30（ g? mL －1），提取次数3次，每次提取20min，室温提取；湖北产和辽宁产五味子提取物清除DPPH自由基的IC50分别为3．79mg? mL －1和7．60mg? mL －1。结论五味子提取物具有较强的抗氧化作用，且湖北产五味子提取物的抗氧化作用比辽宁产五味子的强。
通讯机构：
[Wang, Yong-Sheng] U;Univ South China, Coll Publ Hlth, Hengyang 421001, Peoples R China.
关键词：
Nuclease-resistant DNA aptamer;Gold nanoparticles;Resonance light scattering;Conformation change;Uranyl
摘要：
Various methods have been developed in recent years for the determination of uranyl ion by making use of uranyl-specific DNAzymes. However, many of them suffer from hydrolysis by nucleases present in samples such as body fluids. We report here on an uranyl-specific nuclease-resistant DNA aptamer (UApt) as the recognition element, and how gold nanoparticles (AuNPs) can be used as signal reporters in the respective assay. The presence of uranyl ion leads to a conformational change of UApt, and this results in the dispersion of AuNPs and a decrease in the intensity of resonance light scattering (RLS) at around 573.0 nm. The conformational changes were also studied by polyacrylamide gel electrophoresis, circular dichroism, and UV–vis spectroscopy. The RLS signals are linearly related to the concentration of uranyl ion in the 22 to 550 nM range, with a detection limit of 6.7 nM. This method is more simple and robust than others owing to use of a UApt without a ribonucleotide adenosine. It has been successfully applied to the determination of uranyl ion in real samples. We presume that this method may be extended to the determination of other analytes by making use of the corresponding aptamer for the target. The presence of UO22+ leads to the conformation change of UApt, resulting in the dispersion of AuNPs and decrease of the RLS intensity of the system.
