期刊论文

Xu Xiao-na

中文

光谱学与光谱分析

1000-0593

2015

35

12

3471-3474

10.3964/j.issn.1000-0593(2015)12-3471-04

国家自然科学基金项目（11205085） 湖南省自然基金项目（14JJ3101）资助

本校为第一且通讯机构

根据目标物诱导核酸分子构象发生变化,导致体系共振光散射强度增大的原理建立了检测Hg~(2+)的新方法。当溶液中有Hg~(2+)存在时,基于"T-Hg~(2+)-T"特异性结合作用,促使Hg~(2+)特异核酸探针的构象发生变化,由单链状态变为刚性双链结构,使体系共振光散射强度增大。在波长566 nm处,当汞离子浓度在7.2 ×10~(-9)~9×10~(-8)mol? L~(-1)范围内时,体系的共振光散射增强程度△I与汞离子的浓度(c)具有良好的线性关系。其线性方程为△I=5.12c+3.55(r=0.999 5)。将该方法用于环境水样中汞离子的测定,相对标准偏差(RSD)小于1.9%;样品加标回收率为99.4%~104.3%。该方法以Hg~(2+)的高度特异性核酸探针作为识别...

A new label-free resonance light scattering method for the highly selective and sensitive detection of mercury ion was designed. This strategy makes use of the target-induced DNA conformational change to enhance the resonance light scattering intensity leading to an amplified optical signal. The Hg2+ ion, which possesses a unique property to bind specifically to two DNA thymine (T) bases, in the presence of Hg2+, the specific oligonucleotide probes form a conformational reorganization of the oligonucleotide probes from single-chain structure to...