期刊论文

Determination of uranyl based on uranyl specific DNAzyme by a resonance light scattering technique

中文

分析试验室

1000-0720

2016

35

1

12-16

10.13595/j.cnki.issn1000-0720.2016.0003

21177052:国家自然科学基金 2015JJ2122:湖南省自然科学基金 :南华大学大学生研究性学习与创新实验计划项目

本校为第一机构

建立了基于铀特异性DNA酶-AuNPs检测UO_2~(2+)的共振光散射新方法。在pH 4.5的10 mmol/L MES缓冲溶液中,铀特异性DNA酶在铀作为辅助因子的条件下发生自身断链反应,释放出ssDNA,吸附在AuNPs表面,阻止AuNPs在高浓度盐下聚集,使体系的共振光散射强度降低。在2.08 × 10~(-9) ~ 2.00 × 10~(-8) mol/L范围内,散射光强度的改变值与UO_2~(2+)浓度呈良好线性关系,回归方程为ΔI_ = 59.45 + 14761 c (μmol/L),r = 0.9852,检出限为6.23 × 10~(-10) mol/L,加标回收率为95.0%~106.5%,相对标准偏差为(RSD)1.9%~9.1%,方法成功用于实际样品检测。

A novel resonance light scattering (RLS) method has been developed based on a uranyl (UO_2~(2+)) specific DNAzyme and gold nanoparticles (AuNPs). In this strategy, the cleavage of substrate by DNAzyme in the presence of UO_2~(2+) resulted in releasing a single stranded DNA (ssDNA) that could be adsorbed on AuNPs and protected them from aggregation, leading to the change of RLS intensity. The ΔI signal linearly correlated with the concentration of UO_2~(2+) over the range of 2.08 × 10~(-9) ~ 2.00 × 10~(-8) mol/L with a limit of detection of 6...