National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [21177052]; Science and Technology Program of Hunan Province in China [2010SK3039]; Key Discipline (Public Health and Preventive Medicine) in Hunan Province
机构署名：
本校为第一且通讯机构
院系归属：
公共卫生学院
摘要：
A sensitive, lable-free and low cost fluorescence aptasensor was developed for detecting 8-hydroxy-2'-deoxyguanosine (8-0HdG) by using 8-0HdG aptamer (Apt) as a recognition probe and N-methyl mesoporphyrin IX (NMM) as a reporter. The method is based on the conformational switching of a ICE-stabilized G-quadruplex to a 8-OHdG-stabilized one. NMM can selectively bind to K+-stabilized G-quadruplex instead of 8-OHdG-stabilized one. The addition of 8-0HdG in the solution of Apt - K+ ions leads to a sharp change in fluorescence intensity, which showed a good linear response toward 8-0HdG concentrati...
