摘要：
China is experiencing an increasingly serious aging population. Cognitive function is an important factor and guarantee for the quality of life of older people. Therefore, to achieve healthy aging, this study aimed to examine the sequential multiple mediating effects of indoor ventilation frequency and cognitive function on anxiety and self-rated health in the Chinese older people population. Using the 2018 China Longitudinal Health and Longevity Survey (CLHLS) dataset, we finally selected 10,372 Chinese seniors over the age of 65. First, we describe the basic socio-demographic information of the sample population. Second, Spearman correlation analysis was used to determine whether there was a correlation between indoor ventilation frequency, anxiety, self-rated health, and cognitive function among Chinese older people. Finally, the SPSS macro process program was used to complete the sequence multiple mediation analysis. Indoor ventilation frequency, anxiety, self-rated health and cognitive function were significantly correlated (p < 0.01). Indoor ventilation frequency not only has a direct positive impact on the cognitive function of older people (effect = 0.1427; Standard error = 0.0201; 95%CI: LL = 0.1034, UL = 0.1821), but also indirectly affected cognitive function through three pathways: independent mediation of anxiety (effect = 0.0078; Standard error = 0.0021; 95%CI: LL = 0.0041, UL = 0.0121), independent mediating effect of self-rated health (effect = 0.0154; Standard error = 0.0030; 95%CI: LL = 0.0098, UL = 0.0215), and the chain mediating effect between anxiety and self-rated health (effect = 0.0046; Standard error = 0.0009; 95%CI: LL = 0.0029, UL = 0.0065). All projects are self-reported and some results may be biased. In the future, it may be more inspiring to explore more detailed and specific effects of indoor air quality on cognitive function in older people. Studies have shown that indoor ventilation frequency can improve cognitive function by reducing anxiety and improving self-rated health in older people Chinese. Encouraging older adults to increase the frequency of indoor ventilation will benefit their mental health and cognitive function. This study provides empirical evidence for the association between indoor ventilation frequency and cognitive function in older people Chinese adults. We used nationally representative data to investigate the relationship between indoor ventilation frequency and cognitive function and further explored the mediating role of anxiety and self-rated health in Chinese older adults. Indoor ventilation frequency can not only directly affect cognitive function in older people, but also indirectly affect cognitive function through anxiety and self-rated health. Anxiety and self-rated health have a series of mediating effects between indoor ventilation frequency and cognitive function.
作者：
Liang, Cong;Huang, Xinlin;Pu, Yucui;Zhang, Pei;Wang, Rong
期刊：
JOURNAL OF STROKE & CEREBROVASCULAR DISEASES,2025年34(1):108104 ISSN：1052-3057
通讯作者：
Rong Wang
作者机构：
[Zhang, Pei; Liang, Cong] School of Nursing, University of South China, Heng Yang, 421001, China;[Liang, Cong] Yellow River Centre Hospital of the Yellow River Conservancy Commission, Zheng Zhou, 450000, China. Electronic address: 983849974@qq.com;[Huang, Xinlin] The Hong Kong Polytechnic University, Hong Kong, China;[Pu, Yucui] Chongqing Preschool Education College, Chong Qing, China;[Wang, Rong] School of Nursing, University of South China, Heng Yang, 421001, China. Electronic address: wwrr77@163.com
通讯机构：
[Rong Wang] S;School of Nursing, University of South China, Heng Yang, 421001, China
关键词：
Genome-wide association study;Mendelian randomization;Stroke;Walking pace
摘要：
Background Walking pace (WP), a simple physiological indicator, has been found to be strongly associated with a variety of health outcomes in recent years. Among them, the relationship between walking pace and stroke is of particular interest. Given the high morbidity, disability and mortality associated with stroke, identifying modifiable indicators of health, such as walking pace, could help in stroke prevention strategies. However, the causal relationship between WP and stroke risk remains unclear. This study aims to determine the causal relationship between walking pace and risk of stroke using a two-sample Mendelian randomization approach in a European-ancestry population.
Walking pace (WP), a simple physiological indicator, has been found to be strongly associated with a variety of health outcomes in recent years. Among them, the relationship between walking pace and stroke is of particular interest. Given the high morbidity, disability and mortality associated with stroke, identifying modifiable indicators of health, such as walking pace, could help in stroke prevention strategies. However, the causal relationship between WP and stroke risk remains unclear. This study aims to determine the causal relationship between walking pace and risk of stroke using a two-sample Mendelian randomization approach in a European-ancestry population.
Methods In order to evaluate the potential for a causal relationship between WP and stroke in people of European heritage, a two-sample Mendelian randomization (MR) study was carried out. Statistics about the association of single nucleotide polymorphisms (SNPs) with stroke were taken from FinnGen (R8) (n = 284,040), while the UK Biobank genome-wide association studies (GWAS) provided the summary data on the association of SNPs with WP (n = 459,915). The inverse-variance weighted (IVW) method was utilised as the primary strategy to examine the causal connection between WP and stroke. Additionally, complementary analyses were conducted using the MR-Egger and weighted median. In order to identify the potential directional pleiotropy and heterogeneity, the MR-Egger intercept test, the MR-PRESSO test, and Cochran's Q statistic were all carried out. This connection was evaluated using OR with 95% confidence intervals (CIs).
In order to evaluate the potential for a causal relationship between WP and stroke in people of European heritage, a two-sample Mendelian randomization (MR) study was carried out. Statistics about the association of single nucleotide polymorphisms (SNPs) with stroke were taken from FinnGen (R8) (n = 284,040), while the UK Biobank genome-wide association studies (GWAS) provided the summary data on the association of SNPs with WP (n = 459,915). The inverse-variance weighted (IVW) method was utilised as the primary strategy to examine the causal connection between WP and stroke. Additionally, complementary analyses were conducted using the MR-Egger and weighted median. In order to identify the potential directional pleiotropy and heterogeneity, the MR-Egger intercept test, the MR-PRESSO test, and Cochran's Q statistic were all carried out. This connection was evaluated using OR with 95% confidence intervals (CIs).
Results A total of 48 SNPs were identified as valid instrumental variables in our two-sample MR analysis. The result showed that a slower walking pace is associated with a higher risk of stroke (OR = 0.573; 95% CI, 0.383-0.858, P = 0.007). The “leave-one-out” analysis demonstrated that the absence of a single SNP did not affect the robustness of our results. The MR-Egger intercept test indicated that genetic pleiotropy did not introduce bias into the results [intercept = −2.9E−03, SE = 0.008, P = 0.719] and Cochran's Q test revealed no heterogeneity. Therefore, the sensitivity analyses yielded comparable results. Consequently, the results of the sensitivity analyses were consistent.
A total of 48 SNPs were identified as valid instrumental variables in our two-sample MR analysis. The result showed that a slower walking pace is associated with a higher risk of stroke (OR = 0.573; 95% CI, 0.383-0.858, P = 0.007). The “leave-one-out” analysis demonstrated that the absence of a single SNP did not affect the robustness of our results. The MR-Egger intercept test indicated that genetic pleiotropy did not introduce bias into the results [intercept = −2.9E−03, SE = 0.008, P = 0.719] and Cochran's Q test revealed no heterogeneity. Therefore, the sensitivity analyses yielded comparable results. Consequently, the results of the sensitivity analyses were consistent.
Conclusion Our MR study revealed that WP is inversely associated with risk of stroke. These results provided evidence that slower WP causally increased the risk of stroke, recommending that patients with lower WP should have a prompt physical examination and targeted interventions to reduce their risk of stroke and enhance their quality of life.
Our MR study revealed that WP is inversely associated with risk of stroke. These results provided evidence that slower WP causally increased the risk of stroke, recommending that patients with lower WP should have a prompt physical examination and targeted interventions to reduce their risk of stroke and enhance their quality of life.
摘要：
Aim To explore and explain the mechanisms that influence surface acting in nursing students with different characteristics.
To explore and explain the mechanisms that influence surface acting in nursing students with different characteristics.
Background Nurses are now expected to deliver patient-centered care which necessitates the emotional labor. Surface acting, a form of emotional labor, can lead to negative outcomes. Given that nursing students are the backbone of the future nursing profession, there is an urgent need to investigate their surface acting tendencies and identify potential factors for early intervention.
Nurses are now expected to deliver patient-centered care which necessitates the emotional labor. Surface acting, a form of emotional labor, can lead to negative outcomes. Given that nursing students are the backbone of the future nursing profession, there is an urgent need to investigate their surface acting tendencies and identify potential factors for early intervention.
Design A cross-sectional study.
A cross-sectional study.
Methods This study was surveyed in a vocational college in Gansu, China. Participants completed the general information questionnaire, Bem Sex Role Inventory, Professional Identity Questionnaire of Nursing Students and Surface Acting Scale. K-means cluster analysis was performed, followed by random forest algorithm and SHapley Additive exPlanations based on Python program.
This study was surveyed in a vocational college in Gansu, China. Participants completed the general information questionnaire, Bem Sex Role Inventory, Professional Identity Questionnaire of Nursing Students and Surface Acting Scale. K-means cluster analysis was performed, followed by random forest algorithm and SHapley Additive exPlanations based on Python program.
Results A total of 1241 nursing students from vocational college were investigated and were clustered into 4 groups. The five dimensions of professional identity had higher feature importance in all four groups, with professional self-image having the highest feature importance in Cluster 3. Professional self-image and understanding retention benefits and turnover risks were negative predictors of surface acting in all four groups. Social comparison and self-reflection, independence of career choice and social modeling regarding nursing profession were positively correlated with surface acting in specific groups. In Cluster 1, there exists a positive correlation between professional self-image and the constructs of social comparison and self-reflection; as well as a negative correlation between maternal education and understanding of retention benefits and turnover risks.
A total of 1241 nursing students from vocational college were investigated and were clustered into 4 groups. The five dimensions of professional identity had higher feature importance in all four groups, with professional self-image having the highest feature importance in Cluster 3. Professional self-image and understanding retention benefits and turnover risks were negative predictors of surface acting in all four groups. Social comparison and self-reflection, independence of career choice and social modeling regarding nursing profession were positively correlated with surface acting in specific groups. In Cluster 1, there exists a positive correlation between professional self-image and the constructs of social comparison and self-reflection; as well as a negative correlation between maternal education and understanding of retention benefits and turnover risks.
Conclusions Professional identity significantly influences surface acting behaviors among nursing students, with professional self-image serving as a key negative predictor. Positive family conditions, access to educational resources, parental literacy, masculine or feminine gender roles and first-year nursing students, these traits have implications when dimensions of professional identity are used to predict surface acting behaviors.
Professional identity significantly influences surface acting behaviors among nursing students, with professional self-image serving as a key negative predictor. Positive family conditions, access to educational resources, parental literacy, masculine or feminine gender roles and first-year nursing students, these traits have implications when dimensions of professional identity are used to predict surface acting behaviors.
摘要：
BACKGROUND: To investigate the association between indoor ventilation frequency and symptoms of depression and anxiety in older persons. METHODS: A binary logistic regression model was used to analyze the effects of indoor ventilation frequency on depression and anxiety by using data from the 2018 Chinese longitudinal healthy longevity survey (CLHLS). RESULTS: A total of 9,690 older persons with an average age of (83.20 ± 11.27) years were included, including 4,458 males (46.0%) and 5,232 females (54.0%). The average score of indoor ventilation frequency was (6.06 ± 1.98) points, including 770 people (7.9%) with low frequency, 3,066 people (31.6%) with medium frequency, and 5,854 people (60.4%) with high frequency. 842 (8.7%) had symptoms of depression and 204 (2.1%) had symptoms of anxiety. Compared with the older persons with low indoor ventilation frequency, the older persons with higher ventilation frequency had a lower incidence of depression (OR(95%CI) = 1.92 (1.50 ∼ 2.46), 1.51 (1.27 ∼ 1.79); P < 0.001). However indoor ventilation frequency was not associated with anxiety symptoms. CONCLUSION: Indoor ventilation frequency is related to depressive symptoms in older persons. Communities and families should pay more attention to indoor ventilation and intervene in time to promote and improve the mental health of older persons.
期刊：
Journal of Psychosomatic Research,2025年191:112065 ISSN：0022-3999
通讯作者：
Zeng, Ying
作者机构：
[Huang, Mao-Ting; Yi, Wen-Ting; Yan, Jia-Hui; Wang, Yi-Fei; Feng, Ge-Hui; Yue, Qian-Qian; Tang, Tian; Yan, Hui-Ling; Chen, Yun-Shan; Zhao, Ke-Hao; Liu, Min] Department of International and Humanistic Nursing, Hunan Science Popularization Education Base, School of Nursing, Hengyang Medical School, University of South China, Hengyang, China;[Zeng, Ying] Department of International and Humanistic Nursing, Hunan Science Popularization Education Base, School of Nursing, Hengyang Medical School, University of South China, Hengyang, China.;[Zeng, Ying] Hunan Engineering Research Center for Early Diagnosis and Treatment of Liver Cancer, Cancer Research Institute, Hengyang Medical School, University of South China;[Zeng, Ying] Hunan Province Key Laboratory of Tumor Cellular & Molecular Pathology, Cancer Research Institute, Hengyang Medical School, University of South China, Hengyang, China.. Electronic address: zengying2003@126.com
通讯机构：
[Ying Zeng] D;Department of International and Humanistic Nursing, Hunan Science Popularization Education Base, School of Nursing, Hengyang Medical School, University of South China, Hengyang, China.<&wdkj&>Hunan Engineering Research Center for Early Diagnosis and Treatment of Liver Cancer, Cancer Research Institute, Hengyang Medical School, University of South China<&wdkj&>Hunan Province Key Laboratory of Tumor Cellular & Molecular Pathology, Cancer Research Institute, Hengyang Medical School, University of South China, Hengyang, China.
摘要：
Background Anxiety is a common and significant problem in patients who need to undergo colonoscopy. However, the question of which non-pharmacological intervention is the best strategy to reduce anxiety in patients undergoing colonoscopy remains unanswered.
Anxiety is a common and significant problem in patients who need to undergo colonoscopy. However, the question of which non-pharmacological intervention is the best strategy to reduce anxiety in patients undergoing colonoscopy remains unanswered.
Objectives To evaluate and rank the effectiveness of various non-pharmacological interventions for reducing anxiety in patients undergoing colonoscopy in order to identify the most effective strategies.
To evaluate and rank the effectiveness of various non-pharmacological interventions for reducing anxiety in patients undergoing colonoscopy in order to identify the most effective strategies.
Methods We searched PubMed, Cochrane Library, Embase, Web of Science, and Medline for randomized controlled trials published from the database construction to March 2024. The primary outcome was the difference between pre- and post-intervention anxiety means. A network meta-analysis was conducted utilizing the “gemtc” package based on R4.3.0.
We searched PubMed, Cochrane Library, Embase, Web of Science, and Medline for randomized controlled trials published from the database construction to March 2024. The primary outcome was the difference between pre- and post-intervention anxiety means. A network meta-analysis was conducted utilizing the “gemtc” package based on R4.3.0.
Results The analysis encompassed 24 randomized controlled trials, incorporating 2525 participants and evaluating 9 non-pharmacological interventions. All non-pharmacological interventions reduced anxiety in patients undergoing colonoscopy compared to standard care, with music intervention (SMD = -0.52, 95 %CI (−0.84,-0.20)), audiovisual distraction (SMD = -0.54, 95 %CI(−0.96,-0.12)), video information (SMD = -1.47, 95 %CI(−2.03,-0.90)), individual education (SMD = -1.72, 95 %CI(−2.76,-0.70)), and electroacupuncture (SMD = -1.12, 95 %CI(−2.10,-0.13)) having statistically significant effects. SUCRA ranking identified the priority of individual education (SUCRA: 92.5 %) and video information (SUCRA: 87.7 %). Meta-regression and sensitivity analysis further demonstrated the stability of the evidence. The certainty of the evidence was mostly rated as medium to low.
The analysis encompassed 24 randomized controlled trials, incorporating 2525 participants and evaluating 9 non-pharmacological interventions. All non-pharmacological interventions reduced anxiety in patients undergoing colonoscopy compared to standard care, with music intervention (SMD = -0.52, 95 %CI (−0.84,-0.20)), audiovisual distraction (SMD = -0.54, 95 %CI(−0.96,-0.12)), video information (SMD = -1.47, 95 %CI(−2.03,-0.90)), individual education (SMD = -1.72, 95 %CI(−2.76,-0.70)), and electroacupuncture (SMD = -1.12, 95 %CI(−2.10,-0.13)) having statistically significant effects. SUCRA ranking identified the priority of individual education (SUCRA: 92.5 %) and video information (SUCRA: 87.7 %). Meta-regression and sensitivity analysis further demonstrated the stability of the evidence. The certainty of the evidence was mostly rated as medium to low.
Conclusion This review highlights the superior effects of individual education and video information in reducing anxiety in patients undergoing colonoscopy. The findings of our review could provide clinical decision-makers and healthcare practitioners, such as doctors and nurses, with evidence-based practices for selecting interventions to reduce anxiety in patients undergoing colonoscopy.
This review highlights the superior effects of individual education and video information in reducing anxiety in patients undergoing colonoscopy. The findings of our review could provide clinical decision-makers and healthcare practitioners, such as doctors and nurses, with evidence-based practices for selecting interventions to reduce anxiety in patients undergoing colonoscopy.
期刊：
Journal of Orthopaedic Surgery and Research,2025年20(1):1-8 ISSN：1749-799X
通讯作者：
Zhou, YH;Huang, HY
作者机构：
[He, Yulian; Liu, Bo; Tang, Wen; Yuan, Runzhi] Univ South China, Affiliated Hosp 1, Dept Orthoped Ctr, Hengyang Med Sch, Hengyang City 421001, Hunan Prov, Peoples R China.;[Wang, Yiqi] Univ South China, Sch Nursing, Hengyang City 421001, Hunan Prov, Peoples R China.;[Zhou, Yanhui] Univ South China, Affiliated Hosp 1, Hengyang Med Sch, Nursing Dept, 69 Chuanshan Rd, Hengyang City 421001, Hunan Prov, Peoples R China.;[Huang, Huayong; Huang, HY] Univ South China, Affiliated Hosp 1, Dept Emergency Med Ctr, Hengyang Med Sch, 69 Chuanshan Rd, Hengyang City 421001, Hunan Prov, Peoples R China.
通讯机构：
[Huang, HY ; Zhou, YH ] U;Univ South China, Affiliated Hosp 1, Hengyang Med Sch, Nursing Dept, 69 Chuanshan Rd, Hengyang City 421001, Hunan Prov, Peoples R China.;Univ South China, Affiliated Hosp 1, Dept Emergency Med Ctr, Hengyang Med Sch, 69 Chuanshan Rd, Hengyang City 421001, Hunan Prov, Peoples R China.
关键词：
Early rehabilitation;Hospital stay;Postoperative complications;Elderly patients;Hip fracture;A prospective cohort study
摘要：
Hip fractures in the elderly are a major global public health concern, with incidence projected to rise as populations age. Rehabilitation is critical to recovery after hip fracture surgery, but the ideal timing for initiation remains uncertain. While early rehabilitation, within 48 h post-surgery, is associated with better outcomes, its specific impact on hospital stay duration and postoperative complications is not yet conclusively established. This study aims to evaluate the effects of initiating rehabilitation within 48 h after hip fracture surgery on hospital length of stay and postoperative complications, compared to rehabilitation started one-week post-surgery in elderly patients. It is hypothesized that early rehabilitation will significantly reduce hospital stays and decrease the rate of postoperative complications. In this prospective cohort study, patients aged 65 and older are divided into early rehabilitation (within 48 h) and delayed rehabilitation (after one week) groups. Data will be collected using electronic medical records (EMR), standardized clinical tools (Barthel Index, Timed Up and Go), and patient-reported outcome measures (SF-36, EQ-5D). Statistical analyses will include t-tests and chi-square tests for outcome comparison, with multiple regression adjusting for potential confounders such as age, gender, and comorbidities. This study addresses a gap in current research by comparing early versus delayed rehabilitation for elderly hip fracture patients. The findings will contribute to the development of evidence-based rehabilitation protocols aimed at optimizing recovery, reducing complications, and improving the efficient use of medical resources.
摘要：
Background and Objectives To reach the goal of a 95% reduction in Tuberculosis (TB) mortality by 2035 compared to 2015, it may be necessary for different regions to create specific intervention strategies tailored to their respective TB mortality components. This study aimed to investigate the causes of death among TB patients in China's developed cities.
To reach the goal of a 95% reduction in Tuberculosis (TB) mortality by 2035 compared to 2015, it may be necessary for different regions to create specific intervention strategies tailored to their respective TB mortality components. This study aimed to investigate the causes of death among TB patients in China's developed cities.
Methods This retrospective cohort study included 8,851 TB patients registered at Shenzhen's largest TB-designated hospital between 2017 and 2022. The primary causes of death were categorized, and multivariable logistic regression was used to identify independent risk factors for TB mortality.
This retrospective cohort study included 8,851 TB patients registered at Shenzhen's largest TB-designated hospital between 2017 and 2022. The primary causes of death were categorized, and multivariable logistic regression was used to identify independent risk factors for TB mortality.
Results The study revealed a TB mortality rate of 1.6% during the treatment period. Independent risk factors for TB mortality included male gender, older age, lower body mass index (BMI), anemia, and the presence of tumors. Notably, poor treatment adherence was significant contributors to mortality, even in this well-resourced urban environment. Additionally, the progression of the chronic disease emerged as a critical factor in TB-related deaths, particularly among patients with severe underlying conditions.
The study revealed a TB mortality rate of 1.6% during the treatment period. Independent risk factors for TB mortality included male gender, older age, lower body mass index (BMI), anemia, and the presence of tumors. Notably, poor treatment adherence was significant contributors to mortality, even in this well-resourced urban environment. Additionally, the progression of the chronic disease emerged as a critical factor in TB-related deaths, particularly among patients with severe underlying conditions.
Conclusion This study underscores the importance of addressing treatment adherence and healthcare accessibility to reduce TB mortality, even in urban settings with robust healthcare infrastructure. The findings also highlight the need for integrated management of TB and chronic diseases, as well as early identification and management of underlying disease to improve patient outcomes. These insights are crucial for developing targeted interventions that can enhance TB care and reduce mortality in similar urban populations.
This study underscores the importance of addressing treatment adherence and healthcare accessibility to reduce TB mortality, even in urban settings with robust healthcare infrastructure. The findings also highlight the need for integrated management of TB and chronic diseases, as well as early identification and management of underlying disease to improve patient outcomes. These insights are crucial for developing targeted interventions that can enhance TB care and reduce mortality in similar urban populations.
作者机构：
[You, Jia-Chun; Xu, Chao-Yue; Li, Shu-Lin; He, Zhi-Qing] School of Nursing, University of South China Hengyang Medical School, Hengyang, Hunan, China;[Zou, Ping] School of Nursing, Nipissing University, North Bay, Ontario, Canada;[Chen, Xi] School of Nursing, The Hong Kong Polytechnic University School of Nursing, Hong Kong, China;[Huang, Yan-Jin] School of Nursing, University of South China Hengyang Medical School, Hengyang, Hunan, China 2019000050@usc.edu.cn
摘要：
INTRODUCTION: Stroke presents a considerable burden not only to patients but also to their families and society at large. In many instances, stroke patients opt for home rehabilitation, relying on family caregivers for daily assistance. This dynamic significantly influences the physiological, psychological and social well-being of these caregivers. Despite its importance, the phenomenon of self-regulatory fatigue (SRF) among family caregivers has received insufficient attention in the literature. Therefore, the objective of this study is to investigate the levels of SRF, the characteristics of associated symptoms and the trajectories of symptom change experienced by family caregivers of stroke patients. METHODS AND ANALYSIS: This research employs a mixed-methods approach, combining a cross-sectional study with a prospective longitudinal quantitative and qualitative design. The Chinese version of the SRF Scale and the Chinese version of Patient-Reported Outcomes Measurement Information System profile-29 are used to assess SRF, psychological and physiological symptoms, and related functional outcomes among family caregivers of stroke patients. Latent class growth analysis will be employed to model the heterogeneous developmental trajectories of SRF-related symptoms among family caregivers of stroke patients. Reflexive thematic analysis will be employed to analyse, organise and summarise qualitative data, to identify the experiences and management needs related to SRF among family caregivers during home care. Through this comprehensive mixed-methods approach, the study aims to: investigate the levels of SRF experienced by family caregivers of stroke patients, identify patterns and trajectories of related symptoms. The integration of cross-sectional and longitudinal data allows for a thorough examination of both immediate and long-term aspects of caregiver experiences, providing valuable insights into the complex dynamics of SRF in this population. ETHICS AND DISSEMINATION: The study protocol was approved by the Medical Ethics Committee of the University of South China (approval number 2023-NHHL-051). Data collection was authorised by the ethics committees of the First Affiliated Hospital, Second Affiliated Hospital and Nanhua Affiliated Hospital of the University of South China. The results of this study will be disseminated through publication in pertinent peer-reviewed journals, presentation at local and international conferences, and communication with all relevant stakeholders. TRIAL REGISTRATION NUMBER: ChiCTR2400082717.
摘要：
Chlamydia trachomatis Pgp3 protein-induced immunoprotection is effective but incomplete, which requires the suitable adjuvants to enhance its immune response. Within this context, Hepatitis B core virus-like particles (HBc-VLP) emerge as nanoscale protein particles capable of incorporating either endogenous or exogenous antigens or epitopes. In this study, HBc-Pgp3 chimeric protein was accomplished by integrating the identified dominant epitope of the Pgp3 protein into the major immunodominant region of a truncated HBc-VLP, which was realized in the pET28a (+) vector and expressed via the E. coli BL21 expression system. The efficacious expression and purification of the recombinant HBc-Pgp3 facilitated a tripartite immunization regimen in mice. The immunological assessment encompassed the measurement of IgG antibody and cytokine levels through ELISA, alongside flow cytometric analysis of the CD4+ Th1 cell-mediated immune responses within the murine spleen. Comparative analysis revealed that the HBc-Pgp3-vaccinated mice demonstrated superior IgG antibody titers and subtypes relative to the controls. Moreover, the HBc-Pgp3 formulation was instrumental in augmenting IFN-γ production, enhancing the efficiency of Chlamydia muridarum clearance post-challenge, and severity of hydrosalpinx within the lower genital tract. Collectively, these findings illuminate the potential of the novel HBc-Pgp3 chimeric construct as an innovative vaccine candidate, offering augmented immunoprotection against chlamydial infection.
Chlamydia trachomatis Pgp3 protein-induced immunoprotection is effective but incomplete, which requires the suitable adjuvants to enhance its immune response. Within this context, Hepatitis B core virus-like particles (HBc-VLP) emerge as nanoscale protein particles capable of incorporating either endogenous or exogenous antigens or epitopes. In this study, HBc-Pgp3 chimeric protein was accomplished by integrating the identified dominant epitope of the Pgp3 protein into the major immunodominant region of a truncated HBc-VLP, which was realized in the pET28a (+) vector and expressed via the E. coli BL21 expression system. The efficacious expression and purification of the recombinant HBc-Pgp3 facilitated a tripartite immunization regimen in mice. The immunological assessment encompassed the measurement of IgG antibody and cytokine levels through ELISA, alongside flow cytometric analysis of the CD4+ Th1 cell-mediated immune responses within the murine spleen. Comparative analysis revealed that the HBc-Pgp3-vaccinated mice demonstrated superior IgG antibody titers and subtypes relative to the controls. Moreover, the HBc-Pgp3 formulation was instrumental in augmenting IFN-γ production, enhancing the efficiency of Chlamydia muridarum clearance post-challenge, and severity of hydrosalpinx within the lower genital tract. Collectively, these findings illuminate the potential of the novel HBc-Pgp3 chimeric construct as an innovative vaccine candidate, offering augmented immunoprotection against chlamydial infection.
摘要：
Objective This study examined the heterogeneity of illness perceptions in patients with lung cancer and evaluated the mediating role of self-transcendence in the relation between illness perception and demoralization.
This study examined the heterogeneity of illness perceptions in patients with lung cancer and evaluated the mediating role of self-transcendence in the relation between illness perception and demoralization.
Methods A convenience sample of 477 patients with lung cancer was selected from three tertiary hospitals in Wuhan, China, between January and June 2024. Participants completed the Brief Illness Perception Questionnaire, Self-Transcendence Scale, and Demoralization Scale. Data were analyzed using Mplus 8.3 and SPSS 25.0.
A convenience sample of 477 patients with lung cancer was selected from three tertiary hospitals in Wuhan, China, between January and June 2024. Participants completed the Brief Illness Perception Questionnaire, Self-Transcendence Scale, and Demoralization Scale. Data were analyzed using Mplus 8.3 and SPSS 25.0.
Results Three latent illness perception profiles were identified among patients with lung cancer: low (27.25%), moderate (40.04%), and high (32.71%). Mediation analyses revealed a partial mediation effect in the relation between illness perception and demoralization in the low versus moderate (SE = 1.56, 95% CI = 14.71, 20.86) and high versus low illness perception groups (SE = 1.71, 95% CI = 35.44, 42.71).
Three latent illness perception profiles were identified among patients with lung cancer: low (27.25%), moderate (40.04%), and high (32.71%). Mediation analyses revealed a partial mediation effect in the relation between illness perception and demoralization in the low versus moderate (SE = 1.56, 95% CI = 14.71, 20.86) and high versus low illness perception groups (SE = 1.71, 95% CI = 35.44, 42.71).
Conclusions Patients with lung cancer exhibited heterogeneous illness perceptions, and self-transcendence partially mediated the relation between illness perception and demoralization. Promoting self-transcendence may help mitigate the negative impact of illness perceptions on demoralization. Clinical interventions aimed at reducing negative illness perceptions and enhancing self-transcendence should be prioritized in the care of patients with lung cancer.
Patients with lung cancer exhibited heterogeneous illness perceptions, and self-transcendence partially mediated the relation between illness perception and demoralization. Promoting self-transcendence may help mitigate the negative impact of illness perceptions on demoralization. Clinical interventions aimed at reducing negative illness perceptions and enhancing self-transcendence should be prioritized in the care of patients with lung cancer.
摘要：
Purpose The study aimed to identify the profiles of psychological flexibility in patients with cervical cancer and how self-perceived burden networked to different psychological flexibility profiles.
The study aimed to identify the profiles of psychological flexibility in patients with cervical cancer and how self-perceived burden networked to different psychological flexibility profiles.
Methods The Personalized Psychological Flexibility Index and the Self-Perceived Burden for Cancer Patients were used to measure psychological flexibility and self-perceived burden in patients from the “Be Resilient to Cancer” project. Latent profile analysis was used to identify profiles and computer-simulated network analysis was conducted to determine if self-perceived burden networked to any of the psychological flexibility profiles.
The Personalized Psychological Flexibility Index and the Self-Perceived Burden for Cancer Patients were used to measure psychological flexibility and self-perceived burden in patients from the “Be Resilient to Cancer” project. Latent profile analysis was used to identify profiles and computer-simulated network analysis was conducted to determine if self-perceived burden networked to any of the psychological flexibility profiles.
Results A total of 325 patients with cervical cancer were recruited in this study. Mean age was 42.26 years, the majority were married (71%) and 75.4% had early stage disease (I or II). Three profiles of psychological flexibility were identified: moderate avoidance-low acceptance/harnessing (34.8%), high avoidance-moderate acceptance/harnessing (36.9%), low avoidance and high acceptance/harnessing (28.3%). Furthermore, “worrying about damaging family relationships” and “feeling guilty about affecting family's life plans” were the most bridge symptoms as well as the targeted intervention symptoms for alleviating and aggravating the self-perceived burden respectively.
A total of 325 patients with cervical cancer were recruited in this study. Mean age was 42.26 years, the majority were married (71%) and 75.4% had early stage disease (I or II). Three profiles of psychological flexibility were identified: moderate avoidance-low acceptance/harnessing (34.8%), high avoidance-moderate acceptance/harnessing (36.9%), low avoidance and high acceptance/harnessing (28.3%). Furthermore, “worrying about damaging family relationships” and “feeling guilty about affecting family's life plans” were the most bridge symptoms as well as the targeted intervention symptoms for alleviating and aggravating the self-perceived burden respectively.
Conclusion Three patterns of psychological flexibility were recognized with two symptoms might be targeted to improve the self-perceived burden in women with cervical cancer.
Three patterns of psychological flexibility were recognized with two symptoms might be targeted to improve the self-perceived burden in women with cervical cancer.
作者机构：
[Peng, Tong; Huang, Li-Li; Yue, Qian-Qian; Feng, Ge-Hui; Tang, Tian; Zeng, Ying; Meng, Xin-Ru; Zhao, Ke-Hao; Huang, Hui-Lin; Sun, Ying-Xue] Univ South China, Sch Nursing, Hengyang Med Sch, Dept Int & Humanist Nursing,Hunan Sci Popularizat, Hengyang, Hunan, Peoples R China.;[Zeng, Ying] Univ South China, Hengyang Sch Med, Canc Res Inst, Hunan Prov Key Lab Tumor Cellular & Mol Pathol, Hengyang, Peoples R China.
通讯机构：
[Zeng, Y ] U;Univ South China, Sch Nursing, Hengyang Med Sch, Dept Int & Humanist Nursing,Hunan Sci Popularizat, Hengyang, Hunan, Peoples R China.;Univ South China, Hengyang Sch Med, Canc Res Inst, Hunan Prov Key Lab Tumor Cellular & Mol Pathol, Hengyang, Peoples R China.
关键词：
Colonoscopy;Anxiety;Influencing factors;Mental health
摘要：
Background Anxiety in patients undergoing colonoscopy may also result in adverse effects, including altered vital signs such as elevated heart rate and blood pressure, exacerbation of symptoms like bloating and gastrointestinal discomfort, a decline in cooperation and satisfaction, and even colonoscopy failure. However, limited studies have explored the level of anxiety, factors that influence it, and its specific causes. Methods A cross-sectional study was conducted, recruiting 825 patients undergoing colonoscopy in Hunan Province between January and July 2023 using stratified sampling. The Spielberger State-Trait Anxiety Inventory (STAI), a self-designed demographic characteristics questionnaire, and a colonoscopy patient anxiety influencing factor questionnaire were used. The data were analyzed in SPSS (version 26) using Mann-Whitney U, Kruskal-Wallis, and multiple regression analysis tests. Results The final study included 825 participants, of whom 19.8% exhibited mild anxiety, 37.0% exhibited moderate anxiety, and 43.2% exhibited severe anxiety. The results indicated that insomnia (beta=-0.080, p = 0.013), no comorbidities (beta=-0.147, p < 0.001), not smoking or drinking (beta=-0.158, p < 0.001), and poor health (moderate: beta=-0.183, p < 0.001; poor: beta=-0.164, p < 0.001) were negatively associated with anxiety levels. In contrast, marital status (beta = 0.177, p < 0.001), education level (beta = 0.204, p < 0.001), age (beta = 0.114, p = 0.007), medical insurance (Basic Medical Insurance for Urban Residents beta = 0.204, p < 0.001; Commercial medical insurance: beta = 0.112, p < 0.001), care provided by relatives (beta = 0.102, p = 0.002), diarrhoea (beta = 0.089, p = 0.005), occupation (farmers: beta = 0.099, p = 0.009; self-employed: beta = 0.082, p = 0.014), and paternal upbringing (beta = 0.067, p = 0.034) were positively correlated with anxiety. Several factors had a greater impact on the anxiety level of the patients: education level (beta = 0.204), health status (moderate: beta=-0.183; not good: beta=-0.164), and marital status (beta = 0.177). It probably because higher education levels may increase awareness of potential risks associated with colonoscopy, contributing to greater anxiety. The five common reasons for anxiety included the presence of bloody faeces, enemas, need for further treatment, lack of timely feedback from the physician, lack of an accurate diagnosis. Conclusion The level of anxiety experienced by patients during the colonoscopy phase was more severe and should be alleviated with targeted interventions based on the cause of anxiety, such as pre-procedural counseling, patient education materials, and enhanced communication with healthcare providers.
期刊：
International Journal of Medical Informatics,2025年195:105738 ISSN：1386-5056
通讯作者：
Hu, Li;Zeng, GQ
作者机构：
[Zeng, Mi; Wang, Manyi; Hu, Li; Zeng, Guqing; Li, Zhuolan; Wu, Jingjing; Zeng, Qingqing] Univ South China, Hengyang Med Sch, Sch Nursing, 28 Changsheng Rd West, Hengyang 421001, Hunan, Peoples R China.;[Zeng, Mi; Wang, Manyi; Zeng, Guqing; Li, Zhuolan; Wu, Jingjing; Hu, Li] Univ South China, Sch Enterprise Cooperat Innovat & Entrepreneurship, Hunan Lantern Med Technol Co Ltd, Hengyang, Peoples R China.;[Gui, Sijie] Univ South China, Affiliated Hosp 1, Dept Orthoped & Trauma, Hengyang, Peoples R China.;[Zeng, Qingqing] Army Med Univ, Affiliated Hosp 1, Hosp Chinese Peoples Liberat Army 958, Jiangbei Campus,29 Jianxindong St, Chongqing 400038, Peoples R China.;[Miao, Wanyu] Chongqing Univ Technol, Coll Comp Sci & Engn, Chongqing, Peoples R China.
通讯机构：
[Hu, L; Zeng, GQ ] U;Univ South China, Hengyang Med Sch, Sch Nursing, 28 Changsheng Rd West, Hengyang 421001, Hunan, Peoples R China.
关键词：
Hip fractures;Re-fracture;Automatic machine learning;Prediction models
摘要：
Objective The aim of study was to construct a postoperative re-fracture prediction model for elderly hip fracture patients using an automated machine learning algorithm to provide a basis for early identification of patients with high risk of re-fracture occurrence.
The aim of study was to construct a postoperative re-fracture prediction model for elderly hip fracture patients using an automated machine learning algorithm to provide a basis for early identification of patients with high risk of re-fracture occurrence.
Methods Clinical data were collected and subjected to univariate and multivariate analyses to determine the independent risk factors affecting postoperative re-fracture of hip fracture in the elderly. The collected data were divided into training and validation sets in a ratio of 7:3, AutoGluon was applied to construct LightGBMXT, LightGBM, RandomForestGini, RandomForestEntr, CatBoost, NeuralNetFastAI, XGBoost, NeuralNetTorch, LightGBMLarge and WeightedEnsemble_L2 prediction models, and the constructed models were evaluated using evaluation indicators. The models were externally validated and the model with the best prediction performance was selected.
Clinical data were collected and subjected to univariate and multivariate analyses to determine the independent risk factors affecting postoperative re-fracture of hip fracture in the elderly. The collected data were divided into training and validation sets in a ratio of 7:3, AutoGluon was applied to construct LightGBMXT, LightGBM, RandomForestGini, RandomForestEntr, CatBoost, NeuralNetFastAI, XGBoost, NeuralNetTorch, LightGBMLarge and WeightedEnsemble_L2 prediction models, and the constructed models were evaluated using evaluation indicators. The models were externally validated and the model with the best prediction performance was selected.
Results The incidence of postoperative re-fracture was about 11.7%, and age, comorbid diabetes mellitus, comorbid osteoporosis, rehabilitation exercise status, and preoperative total protein level were considered as independent risk factors. The top three models in terms of AUC values in the training set were WeightedEnsemble_L2 (0.9671), XGBoost (0.9636), and LightGBM (0.9626), the WeightedEnsemble_L2 (0.9759) was best in the external validation. Based on the AUC and other evaluation indicators, WeightedEnsemble_L2 was considered the model with the best prediction performance.
The incidence of postoperative re-fracture was about 11.7%, and age, comorbid diabetes mellitus, comorbid osteoporosis, rehabilitation exercise status, and preoperative total protein level were considered as independent risk factors. The top three models in terms of AUC values in the training set were WeightedEnsemble_L2 (0.9671), XGBoost (0.9636), and LightGBM (0.9626), the WeightedEnsemble_L2 (0.9759) was best in the external validation. Based on the AUC and other evaluation indicators, WeightedEnsemble_L2 was considered the model with the best prediction performance.
Conclusion The constructed model is highly generalizable and applicable, and can be used as an effective tool for healthcare professionals to assess and manage patients’ risk of re-fracture.
The constructed model is highly generalizable and applicable, and can be used as an effective tool for healthcare professionals to assess and manage patients’ risk of re-fracture.
作者机构：
[Su, Yinhua; Li, Zhongyu; Su, YH; Li, ZY; Guo, Yu; Yu, Huixi; Li, Hanbing; Shangguan, Fuliang; Liu, Hui] Hengyang Med Sch, Univ South China, Sch Nursing, Hengyang, Hunan, Peoples R China.;[Liu, Fen] Univ South China, Affliated Hosp 1, Hengyang, Hunan, Peoples R China.
通讯机构：
[Su, YH; Li, ZY ] H;Hengyang Med Sch, Univ South China, Sch Nursing, Hengyang, Hunan, Peoples R China.
摘要：
BACKGROUND: Time-restricted eating (TRE) manages weight effectively, but choosing how long and what time window remain debatable. Although an 8:00 a.m. to 16:00 p.m. time frame is reported to show positive results in most weight loss trial, its safety and efficacy in overweight and obese women with polycystic ovary syndrome (PCOS) is uncertain. This randomized controlled trial is conducted to evaluate the safety and efficacy of TRE in specific populations. OBJECTIVE: This study aims to assess the 6-month effects of TRE on weight change, metabolic improvement, reproductive recovery, and health-related quality of life in overweight and obese women with polycystic ovary syndrome (PCOS), compared to those who did not receive TRE. METHODS: This randomized controlled trial will enroll 96 overweight and obese women with polycystic ovary syndrome (PCOS), who will be randomly assigned to either a TRE group (with an eating window from 8:00 a.m. to 16:00 p.m.) or a control group (without eating time restrictions), with 49 participants in each group. Evaluators and data analysts will remain blinded to group allocation throughout the study. The primary outcomes, including changes in weight and body mass index (BMI), will be assessed weekly. Secondary outcomes, encompassing alterations in sex hormones, metabolic parameters, body composition, sleep quality, quality of life, anxiety, and depression, will be evaluated monthly. Compliance and safety will be continuously monitored throughout the study. Additionally, a 6-month follow-up will be conducted at the end of the trial to assess the long-term effects of TRE. Statistical analysis will include the Anderson-Darling test for normality, T-test/Wilcoxon test based on distribution, mixed-effects models for assessing time/group effects, Cox model for time-to-event analysis, repeated ANOVA for change analysis, and sensitivity analysis. All tests will be conducted using appropriate software, with a significance level set at P<0.05. Missing data will be imputed. DISCUSSION: The purpose of this study protocol is to further evaluate the effects of TRE in overweight and obese women with PCOS through a randomized controlled trial (RCT). Findings from this study are expected to provide new dietary intervention strategies for overweight and obese PCOS participants. ETHICS AND DISSEMINATION: This study has received ethics approval from the Medical Ethics Committee of the University of South China (Number: NHHL027). Participants are included after signing informed consent. Results will be submitted for publication in peer-reviewed journals. TRAIL REGISTRATION: Trail registration number: ChiCTR2400086815.
摘要：
目的综合分析国内外头颈癌患者张口困难相关评估工具,为头颈癌患者张口困难评估提供参考。方法系统检索PubMed、Web of Science、CINAHL、Medline、SinoMed、中国知网、万方及维普数据库,追溯参...展开更多 目的综合分析国内外头颈癌患者张口困难相关评估工具,为头颈癌患者张口困难评估提供参考。方法系统检索PubMed、Web of Science、CINAHL、Medline、SinoMed、中国知网、万方及维普数据库,追溯参考文献并补充检索灰色文献数据库OpenGrey。检索时限为建库至2024年5月18日。结果纳入18篇文献,汇总出11种头颈癌患者张口困难相关评估工具,其中5种已在中国进行本土化。评估角度分为客观角度、主观角度以及主客观相结合角度;评估目的分为张口困难严重程度、张口困难相关生活质量、康复锻炼。结论现有张口困难相关评估工具较多,特征各异,选择时应综合考虑。未来应对相关评估工具进一步验证与完善,也可应用人工智能技术优化或开发新的张口困难评估工具。收起
作者机构：
[Wang, Jiani] Univ South China, Sch Nursing, Hengyang 421001, Peoples R China.;[Rao, Qin] Univ South China, Changsha Cent Hosp, Dept Pulm TB, Changsha 410004, Peoples R China.;[Zhou, Lan] Univ South China, Dept Drug Resistant TB, Changsha Cent Hosp, Changsha 410004, Peoples R China.;[Xiang, Lingling] Univ South China, Dept Extrapulm TB, Changsha Cent Hosp, Changsha 410004, Peoples R China.;[Xi, Mingxia; Xi, MX] Univ South China, Dept Nursing, Changsha Cent Hosp, Changsha 410004, Peoples R China.
通讯机构：
[Xi, MX ] U;Univ South China, Dept Nursing, Changsha Cent Hosp, Changsha 410004, Peoples R China.
关键词：
Continuing care services;Cross-sectional study;Need survey;Pulmonary tuberculosis
摘要：
OBJECTIVE: To understand the relationship between the need for continuing care services and influencing factors, social support, readiness for discharge among discharged pulmonary tuberculosis (PTB) patients. METHODS: A cross-sectional study was conducted among 170 patients from a database of discharged patients with PTB from September 2023 to January 2024. A demographic and disease characteristics questionnaire, continuing care services basic modality questionnaire, continuing care services need questionnaire, the Social Support Rating Scale (SSRS), and the Readiness for Hospital Discharge Scale (RHDS) were used for this investigation. Univariate analysis and multiple linear regression analysis were used to analyze the associated factors. RESULTS: The mean total score for the need for continuing care services among patients with PTB discharged from the hospital was (121.61±22.98). The dimension with the highest score was health education guidance need. Compared to the the original hospital medical personnel, the primary source of care information after discharge was the local medical institutions was statistically significant and negatively correlated with continuing care service need (P=0.005). Social support was positively associated with need for continuing care services (P=0.042). CONCLUSION: Discharged PTB patients had a high degree of continuing care service need. Factors influencing the need for continuing care services are the primary source of care information after discharge was the local medical institutions, the social support. Medical staff need to provide targeted continuing care services based on relevant influencing factors to meet the discharge needs of patients.
