摘要：
Methicillin-resistant Staphylococcus aureus (MRSA) is a widespread pathogen causing clinical infections and is multi-resistant to many antibiotics, making it urgent need to develop novel antibacterials to combat MRSA. Here, a series of novel isoxanthohumol-amine conjugates were synthesized as antibacterials. After bioactivity evaluation, a compound E2 was obtained, which showed excellent antibacterial activity against S. aureus and clinical MRSA isolates (MICs=0.25-1μg/mL), superior to vancomycin, and with negligible hemolysis and good membrane selectivity. Additionally, E2 exhibited fast bacterial killing, less susceptible to resistance, relatively low cytotoxicity, and good plasma stability. Mechanism investigation revealed that E2 can disrupt bacterial membranes by specifically binding to phosphatidylglycerol on the bacterial membrane, thus causing elevated intracellular ROS and leakage of DNA and proteins, and ultimately killing bacteria. Noticeably, E2 displayed a good in vivo safety profile and better in vivo therapeutic efficacy than the same dose of vancomycin, allowing it to be a potential antibacterial to conquer MRSA infections.
期刊：
JOURNAL OF ENDOCRINOLOGICAL INVESTIGATION,2024年:1-16 ISSN：0391-4097
通讯作者：
Zhou, J
作者机构：
[Li, S.; Xu, B.; Kang, B.; Zhou, J.; Fan, S.] Univ South China, Affiliated Hosp 1, Hunan Prov Clin Med Res Ctr Drug Evaluat Major Chr, Hengyang Med Sch, Hengyang 421001, Hunan, Peoples R China.;[Li, S.; Xu, B.; Kang, B.; Zhou, J.; Fan, S.] Univ South China, Affiliated Hosp 1, Hengyang Clin Pharmacol Res Ctr, Hengyang Med Sch, Hengyang 421001, Hunan, Peoples R China.;[Li, S.; Xu, B.; Kang, B.; Zhou, J.; Fan, S.] Univ South China, Affiliated Hosp 1, Hengyang Med Sch, Hengyang Key Lab Clin Pharmacol, Hengyang 421001, Hunan, Peoples R China.;[Li, S.; Xu, B.; Kang, B.; Zhou, J.; Fan, S.] Univ South China, Affiliated Hosp 1, Hengyang Med Sch, Pharm Dept, Hengyang 421001, Hunan, Peoples R China.;[Xu, B.; Zhou, J.] Univ South China, Sch Pharmaceut Sci, Hengyang Med Sch, Hengyang 421001, Hunan, Peoples R China.
通讯机构：
[Zhou, J ] U;Univ South China, Affiliated Hosp 1, Hunan Prov Clin Med Res Ctr Drug Evaluat Major Chr, Hengyang Med Sch, Hengyang 421001, Hunan, Peoples R China.;Univ South China, Affiliated Hosp 1, Hengyang Clin Pharmacol Res Ctr, Hengyang Med Sch, Hengyang 421001, Hunan, Peoples R China.;Univ South China, Affiliated Hosp 1, Hengyang Med Sch, Hengyang Key Lab Clin Pharmacol, Hengyang 421001, Hunan, Peoples R China.;Univ South China, Affiliated Hosp 1, Hengyang Med Sch, Pharm Dept, Hengyang 421001, Hunan, Peoples R China.
关键词：
Sodium-glucose cotransporter 2 inhibitor;Cancer;Dapagliflozin;Bladder cancer;Breast cancer;Renal cancer
摘要：
BACKGROUND: The effect of sodium-glucose cotransporter 2 (SGLT2) inhibitors on cancer has yet to be fully elucidated. OBJECTIVE: This systematic review and meta-analysis investigated the effects of SGLT2 inhibitors on cancer. METHODS: We searched the PubMed and ClinicalTrials.gov databases up to July 15, 2023, to identify eligible randomized, double-blind, placebo-controlled trials that lasted at least ≥24weeks. The primary outcome was the overall cancer incidence, and the secondary outcomes were the incidences of various types of cancer. We used the Mantel-Haenszel method, fixed effects model, risk ratio (RR) and 95% confidence interval (CI) to analyze dichotomous variables. Subgroup analysis was performed based on the SGLT2 inhibitor type, baseline conditions, and follow-up duration. All meta-analyses were performed using RevMan5.4.1 and Stata MP 16.0. RESULTS: A total of 58 publications (59 trials) were included, comprising 113,909 participants with type 2 diabetes mellitus and/or chronic kidney disease and/or high cardiovascular risk and/or heart failure (SGLT2 inhibitor group, 63864; placebo group, 50045). Compared to the placebo SGLT2 inhibitors did not significantly increase the overall incidence of cancer (RR 1.01; 95% CI 0.94-1.08; p = 0.82). However, ertugliflozin did significantly increase the overall incidence of cancer (RR 1.29; 95% CI 1.01-1.64; p = 0.04). SGLT2 inhibitors did not increase the risks of bladder or breast cancer. However, dapagliflozin did significantly reduce the risk of bladder cancer by 47% (RR 0.53; 95% CI 0.35-0.81; p = 0.003). SGLT2 inhibitors had no significant effect on the risks of gastrointestinal, thyroid, skin, respiratory, prostate, uterine/endometrial, hepatic and pancreatic cancers. Dapagliflozin reduced the risk of respiratory cancer by 26% (RR 0.74; 95% CI 0.55-1.00; p = 0.05). SGLT2 inhibitors (particularly mediated by dapagliflozin and ertugliflozin but not statistically significant) were associated with a greater risk of renal cancer than the placebo (RR 1.39; 95% CI 1.04-1.87; p = 0.03). CONCLUSION: SGLT2 inhibitors did not significantly increase the overall risk of cancer or the risks of bladder and breast cancers. However, the higher risk of renal cancer associated with SGLT2 inhibitors warrants concern.
期刊：
Journal of Environmental Radioactivity,2024年272:107331 ISSN：0265-931X
通讯作者：
Hong, CS
作者机构：
[Wen, Jiale; Xie, Bingbing; Hong, CS; Hong, Changshou; Wang, Hong; Wang, Yuhang] Univ South China, Sch Resources Environm & Safety Engn, Hengyang 421001, Peoples R China.;[Wang, Hong] Univ South China, Sch Nucl Sci & Technol, Hengyang 421001, Peoples R China.;[Wen, Jiale; Xie, Bingbing; Hong, Changshou; Wang, Hong; Wang, Yuhang] Univ South China, Hunan Prov Engn Technol Res Ctr Uranium Tailings T, Hengyang 421001, Peoples R China.
通讯机构：
[Hong, CS ] U;Univ South China, Sch Resources Environm & Safety Engn, Hengyang 421001, Peoples R China.
关键词：
Numerical simulation;Radon concentration field;Ramp;Temperature field
摘要：
By introducing the parameters of radon exhalation rate and radon diffusion coefficient, the distribution of radon concentration field on ramp under the condition of superposition of temperature field and flow field is simulated. The simulation results show that the distribution of radon concentration in the ramp under the condition of low-speed ventilation is greatly affected by the temperature field and flow field, and the change of radon exhalation caused by temperature is the main factor leading to the change of radon concentration in the ramp. The change of temperature will cause the overall increase of radon concentration in the ramp. Under the condition of constant flow field, the radon concentration in the chamber is more than two times higher than the average radon concentration in the ramp. Some areas severely exceeded the limit.
摘要：
Background: SN-38, recognized as the primary active derivative of the pivotal chemotherapeutic agent CPT-11, demonstrates substantially enhanced efficacy in colorectal cancer (CRC) management compared to CPT-11. Nonetheless, challenges such as low stability, inadequate aqueous solubility, limited bioavailability, and nonspecific targeting to cancer cells hinder its clinical adoption. In the present research, we synthesized SN-38loaded liposomes cloaked with macrophage membranes (SN-38@MM-LPs) to assess their therapeutic potential and safety profile in addressing CRC. Methods: SN-38@MM-LPs were synthesized using an incubation extrusion technique, combining a macrophage membrane with liposomes (LPs). It was characterized by size, zeta potential, transmission electron microscopy observations, polydispersity index and coomassie bright blue staining. CCK-8, EdU, and flow cytometry assays were performed to evaluate the viability and apoptosis rates of HCT116 and HCT8 cells after treatment with SN38@MM-LPs. A cellular uptake assay was conducted to evaluate the internalization of SN-38@MM-LPs in vitro. Moreover, the biodistribution, therapeutic efficacy, and safety of SN-38@MM-LPs were further assessed in orthotopic HCT116 xenograft model mice. Results: Characterization results revealed that SN-38@MM-LPs possess a spherical morphology with a consistent size distribution (129 nm) and a drug loading efficiency of 5.54 +/- 0.73%. SN-38 curtailed the growth and promoted apoptosis in both HCT8 and HCT116 cells. The impact of SN-38 was accentuated when delivered via SN-38@LPs and SN-38@MM-LPs. Notably, in the orthotopic xenograft model, SN-38@MM-LPs manifested superior tumor-targeting capabilities and therapeutic outcomes. Additionally, SN-38@MM-LPs presented negligible hepatic toxicity. Conclusions: SN-38@MM-LPs showcased potent and targeted antitumor actions in CRC. Consequently, SN38@MM-LPs emerge as a potential nanoparticle formulation that could amplify the antitumor efficacy of SN38, simultaneously mitigating liver toxicity concerns.
期刊：
NEW JOURNAL OF CHEMISTRY,2024年48(6):2855-2865 ISSN：1144-0546
通讯作者：
Sun, YK;Sun, Yunkai;Yang, PF
作者机构：
[Ding, Yi; Sun, Yunkai] Changzhou Inst Technol, Sch Chem Engn & Mat, Changzhou 213032, Peoples R China.;[Yang, Pengfei; Liu, Cheng; Sun, Yunkai; Yin, Na] Univ South China, Sch Chem & Chem Engn, Hengyang 421001, Peoples R China.;[Ding, Yi; Sun, Yunkai] Changzhou Inst Technol, Ind Coll Carbon Fiber & New Mat, Changzhou 213032, Peoples R China.
通讯机构：
[Sun, YK ] C;[Sun, YK; Yang, PF ] U;Changzhou Inst Technol, Sch Chem Engn & Mat, Changzhou 213032, Peoples R China.;Univ South China, Sch Chem & Chem Engn, Hengyang 421001, Peoples R China.;Changzhou Inst Technol, Ind Coll Carbon Fiber & New Mat, Changzhou 213032, Peoples R China.
摘要：
Nanoscale zero-valent iron (nZVI) was cured by vulcanization and biochar functionalization to overcome the tendency of oxidation and aggregation, and the sulfurized nano-zero-valent iron functional material supported by amino biochar (SnZVI-BC-NH2) was obtained, which was used to remove U(VI) from wastewater in this study. A variety of measurements (SEM, TEM, XRD, BET, VSM, FT-IR, and XPS) were used to characterize the morphology, structure, magnetic properties, characteristic functional groups and chemical bonds of SnZVI-BC-NH2. Analysis results confirm that the aminated biochar can support sulfurized nZVI well. The good kinetics, thermodynamics, and large removal capacity data (at pH 6, T = 298 K, Q(m) = 158.1 mg g(-1)) indicate that SnZVI-BC-NH2 has excellent removal performance for U(VI), and the experimental data are in good agreement with the pseudo-second-order kinetic model. Cycling and anti-oxidation tests demonstrate that the FeSx shell plays a crucial role in enhancing the removal of SnZVI-BC-NH2 on U(VI). XPS and FT-IR analysis results show that SnZVI-BC-NH2 removes U(VI) through the synergistic effect of adsorption and reduction.
作者：
Goh, MeeiChyn;Du, Meng;Peng, Wang Rui;Saw, Phei Er;Chen, Zhiyi
期刊：
Drug Delivery,2024年31(1):2300945 ISSN：1071-7544
通讯作者：
Chen, ZY;Saw, PE
作者机构：
[Peng, Wang Rui; Du, Meng; Chen, Zhiyi; Goh, MeeiChyn] Univ South China, Inst Med Imaging, Hengyang Med Sch, Hengyang, Peoples R China.;[Peng, Wang Rui; Chen, Zhiyi] Univ South China, Affiliated Hosp 7, Hunan Vet Adm Hosp, Hengyang Med Sch, Changsha, Peoples R China.;[Saw, Phei Er] Sun Yat Sen Univ, Med Res Ctr,Sun Yat Sen Mem Hosp, Guangdong Prov Key Lab Malignant Tumor Epigenet &, Guangdong Hong Kong Joint Lab RNA Med, Guangzhou, Peoples R China.;[Saw, Phei Er; Saw, PE] Sun Yat Sen Mem Hosp, Nanhai Translat Innovat Ctr Precis Immunol, Foshan, Peoples R China.;[Chen, Zhiyi] Univ South China, Affiliated Changsha Cent Hosp, Hengyang Med Sch, Changsha, Peoples R China.
通讯机构：
[Saw, PE ] S;[Chen, ZY ] U;Univ South China, Inst Med Imaging, Hengyang Med Sch, Hengyang, Peoples R China.;Sun Yat Sen Mem Hosp, Nanhai Translat Innovat Ctr Precis Immunol, Foshan, Peoples R China.
关键词：
Hydrogel;transdermal drug delivery;therapeutic carrier;burn wound;tissue regeneration
摘要：
Burn injuries are prevalent and life-threatening forms that contribute significantly to mortality rates due to associated wound infections. The management of burn wounds presents substantial challenges. Hydrogel exhibits tremendous potential as an ideal alternative to traditional wound dressings such as gauze. This is primarily attributed to its three-dimensional (3D) crosslinked polymer network, which possesses a high water content, fostering a moist environment that supports effective burn wound healing. Additionally, hydrogel facilitates the penetration of loaded therapeutic agents throughout the wound surface, combating burn wound pathogens through the hydration effect and thereby enhancing the healing process. However, the presence of eschar formation on burn wounds obstructs the passive diffusion of therapeutics, impairing the efficacy of hydrogel as a wound dressing, particularly in cases of severe burns involving deeper tissue damage. This review focuses on exploring the potential of hydrogel as a carrier for transdermal drug delivery in burn wound treatment. Furthermore, strategies aimed at enhancing the transdermal delivery of therapeutic agents from hydrogel to optimize burn wound healing are also discussed.
摘要：
This review provides an overview of the recent synthesis methods developed and made available for thiophene products preparation. Thiophene being an important sulfur‐containing heterocyclic motif finds use in pharmaceuticals, functional materials, organic chemistry and natural products, its efficient synthesis is equally important. Abstract Thiophene, as an important sulfur‐containing heterocyclic, is widely used in pharmaceuticals, functional materials, and natural products. Furthermore, thiophene is of great interest to synthetic chemists due to its diverse reactivities. Over the past few decades, many synthetic strategies are developed for functionalized thiophenes. Several reviews have been reported. However, there is no review on thiophene synthesis summarized based on the sulfur sources. The current review envisioned to summary the synthesis methods for thiophene with the respect of sulfur sources and give support for choose the appropriate tools to obtain thiophene substrates.
期刊：
JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY,2024年38(1):e23608- ISSN：1095-6670
通讯作者：
Zhenjie Wang<&wdkj&>Zhenjie Wang Zhenjie Wang Zhenjie Wang
作者机构：
Department of Emergency Medicine, The First Affiliated Hospital of Bengbu Medical College, Bengbu, Anhui, China;Institute of Emergency and Critical Care Medicine, The First Affiliated Hospital of Bengbu Medical College, Bengbu, Anhui, China;[Shugen Xu; Shugen Xu Shugen Xu Shugen Xu] Department of Emergency Medicine, The Affiliated Changsha Central Hospital, Hengyang Medical School, University of South China, Changsha, Hunan, China;[Zhenjie Wang; Zhaolei Qiu; Chuanming Zheng; Feng Cheng; Lei Li; Zhipeng Xu; Qi Song; Fulong Zhang; Zhenjie Wang Zhenjie Wang Zhenjie Wang; Zhaolei Qiu Zhaolei Qiu Zhaolei Qiu; Chuanming Zheng Chuanming Zheng Chuanming Zheng; Feng Cheng Feng Cheng Feng Cheng; Lei Li Lei Li Lei Li; Zhipeng Xu Zhipeng Xu Zhipeng Xu; Qi Song Qi Song Qi Song; Fulong Zhang Fulong Zhang Fulong Zhang] Department of Emergency Medicine, The First Affiliated Hospital of Bengbu Medical College, Bengbu, Anhui, China<&wdkj&>Institute of Emergency and Critical Care Medicine, The First Affiliated Hospital of Bengbu Medical College, Bengbu, Anhui, China
通讯机构：
[Zhenjie Wang; Zhenjie Wang Zhenjie Wang Zhenjie Wang] D;Department of Emergency Medicine, The First Affiliated Hospital of Bengbu Medical College, Bengbu, Anhui, China<&wdkj&>Institute of Emergency and Critical Care Medicine, The First Affiliated Hospital of Bengbu Medical College, Bengbu, Anhui, China
摘要：
This study aimed to explore the impact of different pH values of resuscitation fluid on traumatic hemorrhagic shock (THS),focusing on their effects on glycocalyx and inflammation. A rat model of THS was induced by hemorrhage from a left femur fracture, while an oxygen-glucose deprivation/reoxygenation (OGD/R)-induced HULEC-5acell model was considered as an in vitro THSmodel. The lung tissue pathology and glycocalyx structure were assessed through hematoxylin-eosin (H&E) staining and transmission electron microscope examination. The levels of glycocalyx-related factors and inflammation-related factors were determined by enzyme-linked immunosorbent assay (ELISA).The expression of glycocalyx-related proteins, cell junction-related proteins, and proteins involved in the PI3K/Akt/NF-κB signaling pathway was analyzed by western blot. The results showed that both sodium bicarbonate Ringer's solution (BRS)and lactate Ringer's solution (LRS)were effective in restoring mean arterial pressure and heart rate in THS rats. However, LRS has a stronger impact on promoting inflammation and damaging the glycocalyx compared withBRS. In OGD/R-induced HULEC-5a cells, a pH of 7.4 and 6.5 increased inflammation and disrupted the glycocalyx, while a pH of 8.1 had no significant effect on inflammation or glycocalyx. Furthermore, the PI3K/Akt/NF-κBsignaling pathway was activated by fluid resuscitation and different pH values. However, the activating effect of BRS and pH 8.1 on the PI3K/Akt/NF-κBsignaling pathway was milder compared withLRS and pH6.5. In conclusion, an alkaline recovery environment was more beneficial for the treatment of THS.
作者：
Ablikim, M.;Achasov, M. N.;Adlarson, P.;Ai, X. C.;Aliberti, R.;...
期刊：
PHYSICAL REVIEW D,2024年109(1) ISSN：2470-0010
通讯作者：
Ablikim, M
作者机构：
[Yu, B. X.; Yu, G.; Li, Xiaoyu; Hu, T.; Ning, Z.; Zhang, P.; Wen, S. P.; Shi, X.; Sun, G. X.; Ma, M. M.; Zhao, Ling; Chen, T.; Zhu, Z. A.; Xu, G. F.; Wang, K.; Zhou, L. P.; Heng, Y. K.; Ji, Q.; Zhang, J. W.; Rong, G.; Guan, C. Y.; Qi, F. Z.; Wang, Y. F.; Ouyang, Q.; Zhang, A. Q.; Ji, X. B.; Qian, S.; Dong, M. Y.; Zhang, Jiawei; Fang, S. S.; Chang, J. F.; Liu, Z. A.; Yan, X. Q.; Wu, J. F.; Wang, Yaqian; Sun, S. S.; Ding, B.; Fu, C. D.; Lou, X. C.; Lin, T.; Zhang, B. X.; Xing, T. Y.; Cao, G. F.; Wu, L. H.; Zhao, Y. B.; Fu, Y. W.; Zheng, J. P.; Jiang, X. S.; Kiuchi, R.; Gu, M. H.; Lu, Y. P.; Dong, L. Y.; Zhang, H. Q.; Zhao, G.; Wu, L. J.; Li, L. J.; Yang, Yifan; Yuan, Y.; Lu, X. L.; Wu, Z.; Fang, Y.; Luo, X. L.; Ji, X. L.; Zhang, X. M.; Li, L. K.; Li, H. B.; Liang, H.; Huang, Y. P.; Zhang, J. Y.; Yin, J. H.; Batozskaya, V.; Liu, Huanhuan; Song, W. M.; Chen, X. T.; Zhang, J. Z.; He, K. L.; Chen, G.; Ablikim, M.; Liu, C. X.; Chang, W. L.; Zhu, K.; Zhao, J. Z.; Yang, Tao; Mao, Z. P.; Xiao, S. Y.; Lu, J. G.; Liu, P. L.; Jing, M. Q.; Sun, H. K.; Chen, Y. B.; Zhang, Shuihan; Wang, Z.; Ablikim, M; Liu, Fang; K, X.; Zhao, J. Y.; Dong, J.; Liu, K.; Shi, J. Y.; Wang, H. P.; Yuan, C. Z.; Tang, G. Y.; Yuan, S. C.; Zhang, H. Y.; Chen, M. L.; Zhang, Z. H.; Hou, G. Y.; Shen, H. F.; Shao, L. G.; Hou, Z. L.; Sun, Y. Z.; Liu, B. J.; Li, Ke; Zhu, K. J.; Liu, H. M.; Xu, C. F.; Ma, H. L.; Sun, T.; Ye, M.; Xie, Y. G.; Chen, H. S.; Cao, N.; Deng, Z. Y.; Ma, Q. M.; Wang, Z. Y.; Cai, X.; Yuan, X. Q.; Zhang, Y. H.; Ma, R. Q.; Shi, R. S.; Zheng, W. J.; Zou, J. H.; Zhang, Yao; Liu, J. Y.; Li, W. G.; Wang, Y. Q.; Mo, X. H.; Fang, J.; Shen, X. Y.; Ma, X. Y.; Hu, H. M.; Gong, W. X.; Xu, W.; Hu, Y.; Wang, B.; Zhang, B. L.; Ma, J. L.; Wang, Meng; Yang, Y. X.; Miao, H.; Li, F.; Lu, Z. H.; H, X. T.; Yang, H. X.; Qin, Z. H.; Li, G.; Ping, R. G.; Qiu, J. F.; Dai, H. L.; Li, W. D.; Fang, W. X.; Zeng, Y. J.] Inst High Energy Phys, Beijing 100049, Peoples R China.;[Yuan, L.] Beihang Univ, Beijing 100191, Peoples R China.;[Li, Lei] Beijing Inst Petrochem Technol, Beijing 102617, Peoples R China.;[Jaeger, S.; Fritsch, M.; Li, M. H.; Wiedner, U. W.; Pelizaeus, M.; Kuessner, M. K.; Kopf, B.; Wollenberg, L.; Holtmann, T.; de Boer, R. E.; Feldbauer, F.; Heinsius, F. H. H.; Maldaner, S.; Coen, S. C.; Wenzel, C. W.] Bochum Ruhr Univ, D-44780 Bochum, Germany.;[Briere, R. A.] Carnegie Mellon Univ, Pittsburgh, PA 15213 USA.
通讯机构：
[Ablikim, M ] I;Inst High Energy Phys, Beijing 100049, Peoples R China.
摘要：
The Born cross sections and effective form factors of the process e(+) e(-) -> Lambda(Sigma) over bar (0) + c:c: are measured at 14 center-of -mass energy points from 2.3094 to 3.0800 GeV, based on data corresponding to an integrated luminosity of (478.5 +/- 4.8) pb(-1) collected with the BESIII detector. A nonzero Born cross section is observed at the center-of-mass energy of 2.3094 GeV with a statistical significance of more than five standard deviations, and the cross sections at other energies are obtained with improved precision compared to earlier measurements from the BABAR Collaboration. The Born cross-section line shape is described better by a shape considering the strong -interaction effects than by a pQCD motivated functional form.
作者：
Ye, Xu;Wang, Tao;Zhong, Liyuan;Farres, Jaume;Xia, Jiliang;...
期刊：
JOURNAL OF CANCER,2024年15(6):1657-1667 ISSN：1837-9664
通讯作者：
Zeng, Xi;Cao, DL
作者机构：
[Ye, Xu] Cent South Univ, Hunan Canc Hosp, Changsha 410031, Hunan, Peoples R China.;[Ye, Xu] Cent South Univ, Affiliated Canc Hosp, Xiangya Sch Med, Changsha 410031, Hunan, Peoples R China.;[Cao, Deliang; Zeng, Xi; Wang, Tao; Zhong, Liyuan; Xia, Jiliang] Univ South China, Canc Res Inst, Hunan Prov Key Lab Canc Cellular & Mol Pathol, Hengyang Med Coll, 28 W Changsheng Rd, Hengyang 421009, Hunan, Peoples R China.;[Farres, Jaume] Univ Autonoma Barcelona, Dept Biochem & Mol Biol, E-08193 Barcelona, Spain.
通讯机构：
[Cao, DL ; Zeng, X] U;Univ South China, Canc Res Inst, Hunan Prov Key Lab Canc Cellular & Mol Pathol, Hengyang Med Coll, 28 W Changsheng Rd, Hengyang 421009, Hunan, Peoples R China.
关键词：
AKR1B10;Biomarker;Colorectal cancer;Tissue microarrays;and DNA damage
摘要：
Colorectal cancer (CRC) is the leading cause of cancer death, but little is known about its etiopathology. Aldo-keto reductase 1B10 (AKR1B10) protein is primarily expressed in intestinal epithelial cells, but lost in colorectal cancer tissues. This study revealed that AKR1B10 may not be a prognostic but an etiological factor in colorectal tumorigenesis. Using a tissue microarray, we investigated the expression of AKR1B10 in tumor tissues of 592 colorectal cancer patients with a mean follow-up of 25 years. Results exhibited that AKR1B10 protein was undetectable in 374 (63.13%), weakly positive in 146 (24.66%), and positive 72 (12.16%) of 592 tumor tissues. Kaplan-Meier analysis showed that AKR1B10 expression was not correlated with overall survival or disease-free survival. Similar results were obtained in various survival analyses stratified by clinicopathological parameters. AKR1B10 was not correlated with tumor T-pathology, N-pathology, TNM stages, cell differentiation and lymph node/regional/distant metastasis either. However, AKR1B10 silencing in culture cells enhanced carbonyl induced protein and DNA damage; and in ulcerative colitis tissues, AKR1B10 deficiency was associated acrolein-protein lesions. Together this study suggests that AKR1B10 downregulation may not be a prognostic but a carcinogenic factor of colorectal cancer.
摘要：
Tumor is one of the major diseases that endanger people's health. At present, the treatments used for tumor include surgery, chemotherapy, radiotherapy and so on. Nonetheless, the traditional treatments have some disadvantages, such as insufficient treatment effect, liable to cause multidrug resistance, toxicity and side effect. Further research and exploration of tumor treatment schemes are still necessary. As the energy converter of cells, mitochondria are currently considered to be one of the most important targets for the design of new drugs for tumor, cardiovascular and neurological diseases. Nano-drug delivery carriers have the characteristics of being easily modified with active targeting groups, and it can achieve accurate targeted drug delivery to cells and organelles. This paper reviews the application of mitochondrial targeted nanoparticles in tumor diagnosis and treatment from the aspects of inhibiting tumor cell proliferation, promoting tumor cell apoptosis, inhibiting tumor recurrence and metastasis, and inducing cell autophagy.
期刊：
Molecular Systems Design & Engineering,2024年 ISSN：2058-9689
通讯作者：
Shuang-Shuang Long<&wdkj&>Ying-Wu Lin
作者机构：
School of Chemistry and Chemical Engineering, University of South China, Hengyang 421001, China +86 743 8282375;[Lu Yu] High Magnetic Field Laboratory, Chinese Academy of Sciences, Hefei, Anhui 230031, China;[Shu-Qin Gao] Key Lab of Protein Structure and Function of Universities in Hunan Province, University of South China, Hengyang 421001, China;[Yan-Yan Li; Yu Feng; Shuang-Shuang Long] School of Chemistry and Chemical Engineering, University of South China, Hengyang 421001, China;[Ying-Wu Lin] School of Chemistry and Chemical Engineering, University of South China, Hengyang 421001, China<&wdkj&>Key Lab of Protein Structure and Function of Universities in Hunan Province, University of South China, Hengyang 421001, China
通讯机构：
[Shuang-Shuang Long; Ying-Wu Lin] S;School of Chemistry and Chemical Engineering, University of South China, Hengyang 421001, China<&wdkj&>Key Lab of Protein Structure and Function of Universities in Hunan Province, University of South China, Hengyang 421001, China<&wdkj&>School of Chemistry and Chemical Engineering, University of South China, Hengyang 421001, China
摘要：
Human cytochrome c (hCyt c) contains a covalently attached heme group with six-coordination (Met/His) and plays vital biological functions, including electron transfer and peroxidase activity by structural alterations, as well as other functions by interactions with partners such as neuroglobin (Ngb). In this study, we designed and engineered an artificial disulfide bond in hCyt c via double mutations (A51C/G77C) which bridges the Ω-loops C and D. The formation of the intramolecular disulfide bond (Cys51–Cys77) was confirmed by mass spectrometry. The molecular modeling study showed that the disulfide bond did not alter the overall structure, and the local structure where Cys51 was located was well folded into an α-helix. Spectroscopic studies were also performed to probe the effects of the disulfide bond on the protein structure, which revealed that the heme coordination of Met80 was likely weakened. Consequently, the rate of ligand binding and the peroxidase activity were enhanced. Meanwhile, the interaction between hCyt c and Ngb was weakened, as suggested by titration studies. These observations indicate that the dynamic properties of Ω-loops C and D may favor the heme coordination and protein–protein interactions by conformational change, which supports the native functions of hCyt c.
摘要：
The effects of reaction temperature, reaction time, NaOH concentration and dosage on the hydrolysis rate of TBP were investigated. With the optimized conditions, the hydrolysis rate of TBP reached more than 99.5%. The reaction temperature is the greatest impact factor of TBP hydrolysis. As the hydrolysis reaction proceeded, TBP was first hydrolyzed into NaDBP, and then further hydrolyzed into Na2MBP. Three phases were formed after alkaline hydrolysis of TBP/n-dodecane, the n-dodecane (approximate to 70% v%) was at the top phase that could be recycled or reused.
摘要：
An innovative MnO/Ti3C2 MXene heterostructure, consisting of three-dimensional (3D) MnO nanosheet array directly assembled on two-dimensional (2D) Ti3C2 MXene nanosheets, was synthesized via a simple hydrothermal-annealing strategy for uranium elimination. This unique 3D/2D heterostructure derived substantially high specific surface area (311.9 m2/g) and exposed considerable amount of surface oxygen groups, including surface lattice M-O and adsorbed -OH. Through a comprehensive exploration encompassing adsorbent dosage, pH, ionic strength, initial U(VI) concentration, and environmental conditions, the MnO/Ti3C2 MXene heterostructure demonstrated an extraordinary proficiency and specificity in the removal of U(VI). This composite exhibited an outstanding adsorption capacity of 862.1 mg/g, with a removal efficiency of 99.7 % and rapid kinetics (above 90 % within 20 s). The kinetic study indicated the adsorption process followed chemisorption dynamics and single -layer adsorption behavior. Comprehensive analyses validated the structural integrity and confirmed the successful uranium adsorption via strong interaction between UO22+ and surface unsaturated oxygen groups. Notably, the MnO/Ti3C2 MXene maintained excellent recyclability, highlighting its potential for large-scale applications. It emerges as a promising candidate for uranium removal, offering insights for optimizing composite materials in diverse environmental conditions and fostering advancements in water purification technologies.
摘要：
LaFe1-xMnxO3 (0 <= x <= 0.9) samples were synthesized through the sol-gel method. According to X-ray diffraction (XRD) results, with the increase of Mn doping, the crystal structure of the sample changes from orthogonal Pnma structure to rhombohedral R3c structure, and a two-phase coexistence phenomenon exists within the range of 0.4 <= x <= 0.7. Using Mossbauer spectroscopy, the coexistence range of the two phases and the iron content in each phase were identified. At x=0.4, the Pnma phase contribution (91.1%) dominates, with only a slight R3c phase contribution (8.9%). As the amount of Mn doping gradually increases, the R3c phase becomes dominant at x=0.6 (74.6%), and the Pnma phase completely disappears at x=0.8. This work will provide an important perspective for future researchers studying similar phase coexistence systems.
摘要：
In order to improve the utilization of spent fuel, molten salt electrolysis is used to separate lanthanides from spent fuel. The electrochemical reduction mechanism of Ce(III) ions and dynamic properties of Ce(III)/Ce(0) were provided in the LiCl-KCl molten salt by diversified electrochemical techniques. The diffusion coefficient of Ce(III) and the exchange current densities of Ce(III)/Ce(0) on the W electrode were calculated by cyclic vol-tammetry and linear polarization, and the reaction activation energy was calculated to be 30.8 kJ center dot mol-1. The electrochemical behaviors were measured in the LiCl-KCl-CeCl3-K2ZrF6 molten salt on the W electrode and LiCl-KCl-CeCl3 molten salt on the Zr electrode at 753 K. The redox mechanism of Ce(III) ions was studied on the Zr electrode. Compared the reaction at different electrodes, the reduction potential of cerium on the Zr electrode is more correct than that on the W electrode, the underpotential displacement of cerium and zirconium due to the formation of alloy is 0.26 V, which was detecteted using cyclic voltammetry, square wave voltammetry and chronopotentiometry. In addition, the feasibility of extracting Ce on the Zr electrode by potentiostatic elec-trolysis at-1.9 V and-2.2 V in the LiCl-KCl molten salt. The cathodic deposition output was characterized by XRD and SEM-EDS, Ce-Zr solid solution was derived under the different conditions. Simultaneously, the Ce metal was discovered when the deposition potential at-2.2 V. The ICP-OES results showed that the extraction ratio of was about 94.02 % for Ce(III) after potentiostatic electrolysis at-2.2 V for 5 h.
