Aggregation-induced photoluminescence enhancement of protamine-templated gold nanoclusters for 1-hydroxypyrene detection using 9-hydroxyphenanthrene as a sensitizer
作者：
Xue, Jin-Hua;Xiao, Ke-Ping;Wang, Yong-Sheng* ;Liu, Ling;Li, Jia-Qian;...
期刊：
Colloids and Surfaces B: Biointerfaces ,2020年189:110873 ISSN：0927-7765
通讯作者：
Wang, Yong-Sheng
作者机构：
[Li, Jia-Qian; Li, Mei; Qu, Yue-Ning; Liu, Ling; Xue, Jin-Hua; Xiao, Ke-Ping; Wang, Yong-Sheng] Univ South China, Coll Publ Hlth, Hengyang 421001, Peoples R China.;[Xiao, Xi-Lin] Univ South China, Coll Chem & Chem Engn, Hengyang 421001, Peoples R China.
通讯机构：
[Wang, Yong-Sheng] U;Univ South China, Coll Publ Hlth, Hengyang 421001, Peoples R China.
关键词：
1-Hydroxypyrene;9-Hydroxyphenanthreneene;Aggregation-induced photoluminescence enhancement;Human urinary samples;Hydrogen bonding;Protamine-templated gold nanoclusters
摘要：
In this study, we confirmed that protamine-templated gold nanoclusters (PRT-AuNCs) exhibit aggregation-induced emission properties (AIE-PRT-AuNCs). 1-Hydroxypyrene (1−OHPy) further induced the aggregation of AIE-PRT-AuNCs via hydrogen bonding and electrostatic and hydrophobic interactions, resulting in the aggregation-induced photoluminescence enhancement of AIE-PRT-AuNCs. 9-Hydroxyphenanthrene was able to decrease the background signal, thus increasing the sensitivity of the method. Based on these findings, a cost-effective, highly sensitive and selective strategy was proposed for the quantitative detection of 1−OHPy. This method displayed a wide linear range of 0.924 – 74.1 nmol/L with a low detection limit of 0.277 nmol/L, showing great potential for the monitoring of 1−OHPy in human urine. This strategy may provide a theoretical basis for future studies of the AIE properties of metal nanoclusters and their applications in the field of chemical and biological sensing. © 2020 Elsevier B.V.
语种：
英文
展开
A label-free ultrasensitive and selective strategy for Pb(ii) assay by a multifunctional DNA probe-mediated rolling-circle amplified synthesis of the G-quadruplexes
作者：
Wang, Xiao-Feng;Wang, Yong-Sheng* ;Xiao, Xi-Lin;Lan, Wen-Bo;Zhou, Bin;...
期刊：
ANALYTICAL METHODS ,2018年10(25):3081-3088 ISSN：1759-9660
通讯作者：
Wang, Yong-Sheng
作者机构：
[Zhou, Bin; Chen, Si-Han; Xue, Jin-Hua; Wang, Xiao-Feng; Wang, Yong-Sheng] Univ South China, Coll Publ Hlth, Hengyang 421001, Peoples R China.;[Lan, Wen-Bo; Wang, Xiao-Feng] Xiang Nan Univ, Coll Publ Hlth, Chenzhou 423000, Peoples R China.;[Xiao, Xi-Lin] Univ South China, Coll Chem & Chem Engn, Hengyang 421001, Peoples R China.
通讯机构：
[Wang, Yong-Sheng] U;Univ South China, Coll Publ Hlth, Hengyang 421001, Peoples R China.
摘要：
We report a label-free ultrasensitive and selective strategy for Pb(ii) assay by a multifunctional DNA probe (MDP)-mediated rolling-circle amplified synthesis of the G-quadruplexes. The MDP acted as a target recognition probe, a catalytic DNAzyme and a primer of rolling-circle amplification (RCA). The presence of Pb(ii) can induce the conformational switching of the MDP, resulting in the cleavage of the S-DNA in the MDP to release an E-DNA. The released E-DNA then initiated a RCA reaction with a reasonably devised padlock DNA template to produce an accumulated amount of repeated sequences. The RCA product in the present work was designed as a G-rich sequence, which could fold into thousands of G-quadruplex units. The G-quadruplex formed by RCA can specifically bind to NMM to result in an amplified fluorescence signal. Through these cascade amplifications, Pb(ii) ions can be detected at as low as 94.29 pM, which is much lower than those reported in related literature. We expect that this amplification strategy might be helpful in the design of a highly sensitive analytical platform for wide application in environmental and biomedical fields. © The Royal Society of Chemistry.
语种：
英文
展开
Protamine-gold nanoclusters as peroxidase mimics and the selective enhancement of their activity by mercury ions for highly sensitive colorimetric assay of Hg(II)
作者：
Huang, Yan-Qin;Fu, Sha;Wang, Yong-Sheng* ;Xue, Jin-Hua;Xiao, Xi-Lin;...
期刊：
Analytical and Bioanalytical Chemistry ,2018年410(28):7385-7394 ISSN：1618-2642
通讯作者：
Wang, Yong-Sheng
作者机构：
[Zhou, Bin; Chen, Si-Han; Xue, Jin-Hua; Huang, Yan-Qin; Fu, Sha; Wang, Yong-Sheng] Univ South China, Coll Publ Hlth, West Changsheng Rd 28, Hengyang 421001, Hunan, Peoples R China.;[Xiao, Xi-Lin] Univ South China, Coll Chem & Chem Engn, Hengyang 421001, Hunan, Peoples R China.
通讯机构：
[Wang, Yong-Sheng] U;Univ South China, Coll Publ Hlth, West Changsheng Rd 28, Hengyang 421001, Hunan, Peoples R China.
关键词：
Colorimetric sensing;Hg2+-Au0/Au+ interaction;Mercury;Partly oxidized Au species;Peroxidase-like activity;Protamine-gold nanoclusters
摘要：
We certify that protamine-gold nanoclusters (PRT-AuNCs) synthesized by one-pot method exhibit peroxidase-like activity. The catalytic activity of PRT-AuNCs followed typical Michaelis-Menten kinetics and exhibited higher affinity to 3,3',5,5'-tetramethylbenzidine (TMB) as the substrate compared to that of natural horseradish peroxidase. Meanwhile, we found that Hg(II) could dramatically and selectively enhance the peroxidase-like activity of PRT-AuNCs, and the enhanced mechanism by Hg(II) was demonstrated to be generation of the cationic Au species and the partly oxidized Au species (Au(delta+)) by Hg(2+)-Au(0)/Au(+) interaction. Based on this finding, quantitative determinations of Hg(II) via visual observation and absorption spectra were achieved. The proposed strategy displays high selectivity that arises from the strong aurophilic interaction of mercury towards gold. Moreover, the developed method is highly sensitive with a wide linear range and low detection limit of 1.16 nM. This strategy is not only helpful to develop effective nanomaterials-based artificial enzyme mimics but also irradiative to discover new applications of artificial mimic enzymes in bio-detection, medical diagnostics, and biotechnology. Graphical abstract Protamine-gold nanoclusters (PRT-AuNCs) synthesized by one-pot method exhibit peroxidase-like activity. Hg(II) can stimulate the peroxidase-like activity of PRT-AuNCs selectively, enhancing their ability to catalyze the chromogenic reaction of TMB by H2O2.
语种：
英文
展开
Streptavidin and gold nanoparticles-based dual signal amplification for sensitive magnetoelastic sensing of mercury using a specific aptamer probe
作者：
Huang, Yan-Qin;Yin, Ji-Cheng;Wang, Yong-Sheng* ;Xiao, Xi-Lin;Zhou, Bin;...
期刊：
Sensors and Actuators B-Chemical ,2016年235:507-514 ISSN：0925-4005
通讯作者：
Wang, Yong-Sheng
作者机构：
[Zhou, Bin; Yin, Ji-Cheng; Chen, Si-Han; Tang, Xian; Xue, Jin-Hua; Zhu, Yu-Feng; Huang, Yan-Qin; Wang, Yong-Sheng; Wang, Xiao-Feng] Univ South China, Coll Publ Hlth, Hengyang 421001, Peoples R China.;[Yin, Ji-Cheng] Ctr Dis Control & Prevent Zibo City Shandong Prov, Zibo 255000, Peoples R China.;[Xiao, Xi-Lin] Univ South China, Coll Chem & Chem Engn, Hengyang 421001, Peoples R China.
通讯机构：
[Wang, Yong-Sheng] U;Univ South China, Coll Publ Hlth, Hengyang 421001, Peoples R China.
关键词：
Wireless magnetoelastic sensor;Mercury(II) specific aptamer probe;Graphene oxide;Streptavidin;Gold nanoparticles
摘要：
A novel wireless magnetoelastic sensor (WMS) strategy for Hg2+ assay was proposed using a specific aptamer probe and graphene oxide (GO) as an adsorbent. The introduction of Hg2+ results in the release of CDNA-biotin. This one can be adsorbed to the surface of GO that was immobilized on the WMS along with a decrease of the resonance frequency of the sensor. A biotin-streptavidin (SA)-gold nanoparticles( AuNPs) system designed in this work was used for amplifying response signal of the sensor. The signal intensity linearly correlated with Hg2+ concentration ranging from 2.80 to 88.9 nM with a detection limit of 0.885 nM. This WMS has additional advantages of being less time-consuming, low cost and remote query, and can be employed for the real-time and in situ detection of Hg2+ in real samples. The mechanism for structure switching and signal amplification was also studied by transmission electron microscopy and UV-vis spectra. (C) 2016 Elsevier B.V. All rights reserved.
语种：
英文
展开
Determination of metallothioneins by fluorescence and resonance light scattering strategies based on ciprofloxacin–Cu(II) system
作者：
Liu, Lu;Wang, Yong-Sheng;Xue, Jin-Hua;Yang, Hui-Xian;Li, Qiu;...
期刊：
Journal of Luminescence ,2013年138:251-257 ISSN：0022-2313
通讯作者：
Wang, Yong-Song
作者机构：
[Zhou, Bin; Wang, Yong-Song; Yang, Hui-Xian; Yin, Ji-Cheng; Xue, Jin-Hua; Liu, Lu; Wang, Yong-Sheng; Li, Qiu; Wang, Jia-Cheng] Univ South China, Coll Publ Hlth, Hengyang 421001, Peoples R China.;[Xiao, Xi-Lin] Univ South China, Coll Chem & Chem Engn, Hengyang 421001, Peoples R China.
通讯机构：
[Wang, Yong-Song] U;Univ South China, Coll Publ Hlth, Hengyang 421001, Peoples R China.
关键词：
Metallothioneins;Ciprofloxacin-copper chelate;Fluorescence;Resonance light scattering
摘要：
Based on ciprofloxacin (CIP)-Cu(II) system, the novel methods for the detection of metallothioneins (MTs) have been developed by fluorescence (FL) and resonance light scattering (RLS) strategies. The FL strategy avoids the label and derivatization steps in common methods, while the RLS strategy can be applied for determining bio-macromolecules and small molecules without native fluorescence. The response signals linearly correlated with the concentration of MTs over the ranges of 1.03×10<sup>-8</sup>-1.23×10<sup>-6</sup> mol L<sup>-1</sup> for FL, and of 2.56×10<sup>-7</sup>-1.54×10 <sup>-6</sup> mol L<sup>-1</sup> for RLS. The limits of detection (LOD) are 3.1×10<sup>-9</sup> mol L<sup>-1</sup> for FL and 7.68×10 <sup>-8</sup> mol L<sup>-1</sup> for RLS. This study represents the comparison of these two methods using the same CIP-Cu<sup>2+</sup>-MTs system. They not only allow practical application for MTs detection but also serve as a potential choice for the operators according to their concrete needs. In addition, the mechanisms for FL and RLS enhancement of the system were also discussed. ©2013 Elsevier B.V.
语种：
英文
展开
A wireless magnetoelastic sensor for uranyl using DNAzyme–graphene oxide and gold nanoparticles-based amplification
作者：
Yin, Ji-Cheng;Wang, Yong-Sheng;Zhou, Bin;Xiao, Xi-Lin;Xue, Jin-Hua;...
期刊：
Sensors and Actuators B-Chemical ,2013年188:147-155 ISSN：0925-4005
通讯作者：
Wang, Yong-Song
作者机构：
[Zhou, Bin; Wang, Yong-Song; Yin, Ji-Cheng; Qian, Qiu-Mei; Xue, Jin-Hua; Wang, Yong-Sheng; Wang, Jia-Cheng] Univ South China, Coll Publ Hlth, Hengyang 421001, Peoples R China.;[Xiao, Xi-Lin] Univ South China, Coll Chem & Chem Engn, Hengyang 421001, Peoples R China.
通讯机构：
[Wang, Yong-Song] U;Univ South China, Coll Publ Hlth, Hengyang 421001, Peoples R China.
关键词：
DNAzyme;Gold nanoparticles;Graphene oxide;Uranyl;Wireless magnetoelastic sensor
摘要：
A wireless magnetoelastic sensor (WMS) for uranyl detection has been developed using DNAzyme-graphene oxide (GO) and gold nanoparticles (AuNPs)-based amplification. The WMS is fabricated by immobilizing a layer of GO film on a magnetoelastic ribbon-like sensor. When the substrate strand of DNAzyme was cleaved in the presence of UO<inf>2</inf><sup>2+</sup>, the single-stranded DNA (ssDNA) was adsorbed onto the GO film of the WMS, resulting in a decrease of resonance frequency of the WMS. HAuCl<inf>4</inf> can be reduced by ascorbic acid (AA) to atom Au coated on AuNPs in cetyltrimethyl ammonium bromide (CTMAB) solution, leading to the signal amplification of the system. When the molar ratio of AA and HAuCl<inf>4</inf> was 3:2, the response signals linearly correlated with the concentration of UO<inf>2</inf><sup>2+</sup> over the range of 9.57 ×10<sup>-9</sup> to 1.20 ×10<sup>-7</sup> M in the MES buffer solution of pH 5.0 with a detection limit of 2.87 ×10<sup>-9</sup> M. The proposed WMS has additional advantages of being less time-consuming, low cost and remote query, and avoids the interference of some metals such as Cu<sup>2+</sup>, Fe<sup>2+</sup>, Fe<sup>3+</sup>, Hg<sup>2+</sup>, and Tb <sup>3+</sup>. It has been successfully applied for detecting UO <inf>2</inf><sup>2+</sup> in real samples with a satisfactory result. A polyacrylamide gel electrophoresis (PAGE) assay, scanning electron microscopy (SEM) and UV-vis spectra were used to confirm the mechanism for structure switching and signal amplification. ©2013 Elsevier B.V.
语种：
英文
展开
GC-MS和HELP法分析药对陈皮-青皮与其单味药挥发油成分
作者：
徐小娜;蒋军辉;王永生;杨慧仙
期刊：
分析测试学报 ,2012年31(10):1277-1281 ISSN：1004-4957
作者机构：
[徐小娜; 王永生; 杨慧仙] 南华大学公共卫生学院;[蒋军辉] 南华大学化学化工学院
关键词：
药对;陈皮-青皮;气相色谱-质谱;直观推导式演进特征投影法;挥发油;程序升温保留指数
摘要：
采用水蒸气蒸馏法提取药对陈皮-青皮与其单味药的挥发油成分,利用气相色谱-质谱法(GC-MS)和化学计量学多元分辨方法——直观推导式演进特征投影法(HELP),同时结合程序升温保留指数(PTRI)进行挥发油成分定性定量分析。分别在陈皮、青皮和药对陈皮-青皮的挥发油中鉴定出27、27和23个成分,定性组分含量分别占三者挥发油总含量的93.64%/96.45%和85.28%,共有化合物19种;主要化学成分为D-柠檬烯、y-萜品烯、芳樟醇和а-法呢烯。
语种：
中文
展开
Spectroscopic Study on the Interaction of Pyronine Y with Nucleic Acids and Its Analytical Application
作者：
Xiao, Xilin;Huang, Mingyuan;Wang, Yongsheng* ;Gan, Lou;Zhu, Guizhi;...
期刊：
Spectroscopy Letters ,2012年45(8):569-574 ISSN：0038-7010
通讯作者：
Wang, Yongsheng
作者机构：
[Wang, Yongsheng; Xiao, Xilin; Xue, Jinhua] Univ S China, Coll Publ Hlth, Hengyang 421001, Hunan, Peoples R China.;[Wang, Yongsheng; Xiao, Xilin; Xue, Jinhua] Univ S China, Coll Chem & Chem Engn, Hengyang 421001, Hunan, Peoples R China.;[Huang, Mingyuan] Guangdong Med Coll, Sch Publ Hlth, Dongguan, Guangdong, Peoples R China.;[Gan, Lou] So Med Univ, Sch Publ Hlth & Trop Med, Guangzhou, Guangdong, Peoples R China.;[Zhu, Guizhi] Univ Florida, Dept Physiol, Shands Canc Ctr, UF Genet Inst, Gainesville, FL USA.
通讯机构：
[Wang, Yongsheng] U;Univ S China, Coll Publ Hlth, Hengyang 421001, Hunan, Peoples R China.
关键词：
nucleic acids;pyronine Y;resonance Rayleigh scattering
摘要：
The interaction of pyronine Y (PY) with nucleic acids was studied by resonance Rayleigh scattering (RRS) for nucleic acid detection. The enhanced RRS intensity of nucleic acids reacted with PY was proportional to the concentration of nucleic acids in the ranges of 27.0-625ngml -1 for fish sperm DNA, 39.0-500ngml - for calf thymus DNA, and 59.0-375ngml -1 for yeast RNA. The limits of determination were 0.2ngml -1 for fish sperm DNA, 0.6ngml -1 for calf thymus DNA, and 0.7ngml 1 for yeast RNA. The method had been successfully applied to the quick determination of nucleic acids in synthetic and natural samples. © 2012 Copyright Taylor and Francis Group, LLC.
语种：
英文
展开
A square wave voltammetric method for the detection of microorganism populations using a MWNT-modified glassy carbon electrode
作者：
Xiao, Xilin* ;Zhu, Guizhi;Liao, Lifu;Liu, Bo;Yuan, Yali;...
期刊：
Electrochimica Acta ,2012年74:105-110 ISSN：0013-4686
通讯作者：
Xiao, Xilin
作者机构：
[Wu, Yimou; Yuan, Yali; Wang, Yongsheng; He, Jun; Xiao, Xilin; Liao, Lifu; He, Bo] Univ S China, Coll Chem & Chem Engn, Hengyang 421001, Hunan, Peoples R China.;[Wu, Yimou; Yuan, Yali; Wang, Yongsheng; He, Jun; Xiao, Xilin; Liao, Lifu; He, Bo] Univ S China, Coll Publ Hlth, Pathogen Biol Inst, Hengyang 421001, Hunan, Peoples R China.;[Zhu, Guizhi; Liu, Bo; Xiao, Xilin] Univ Florida, Ctr Res Bio Nano Interface, Dept Chem & Physiol, Gainesville, FL 32611 USA.;[Zhu, Guizhi; Liu, Bo; Xiao, Xilin] Univ Florida, Dept Funct Genom, Strands Canc Ctr, UF Genet Inst, Gainesville, FL 32611 USA.;[Zhu, Guizhi; Liu, Bo; Xiao, Xilin] Univ Florida, McKnight Brain Inst, Gainesville, FL 32611 USA.
通讯机构：
[Xiao, Xilin] U;Univ S China, Coll Chem & Chem Engn, Hengyang 421001, Hunan, Peoples R China.
关键词：
Square wave voltammetry (SWV);Cyclic voltammetry (CV);Multiwalled carbon nanotube (MWNT);Glassy carbon electrode;Escherichia coli O157:H7
摘要：
A novel method for determination of trace amounts of microorganism populations in solution was developed by using a multiwalled carbon nanotube (MWNT) modified glassy carbon electrode and square wave voltammetry (SWV). The simultaneous combination of MWNT and SWV allowed the electrochemical signal of electro-active materials in Escherichia coli O157:H7 (E. coli) to be dramatically amplified. Compared with a bare glassy carbon electrode, the MWNT-modified glassy carbon electrode showed catalytic properties in the oxidation of electro-active materials on cell surfaces. Moreover, SWV was proved to be more sensitive than cyclic voltammetry (CV) for investigation of the electrochemical behavior of cells. In this paper, a linear relationship was obtained between the SWV peak current and the cell concentration in the range 2 × 102–2 × 108 cell mL−1 with a detection limit of 2 × 102 cell mL−1. The effect of antibiotic drug Gentamycin Sulfate injection (GSI) on the growth of E. coli was also investigated.
语种：
英文
展开
A gold nanoparticles-modified aptamer beacon for urinary adenosine detection based on structure-switching/fluorescence-“turning on” mechanism
作者：
Zhang, Jin-Quan;Wang, Yong-Sheng* ;Xue, Jin-Hua;He, Yan;Yang, Hui-Xian;...
期刊：
Journal of Pharmaceutical and Biomedical Analysis ,2012年70:362-368 ISSN：0731-7085
通讯作者：
Wang, Yong-Sheng
作者机构：
[Yang, Hui-Xian; Shi, Lin-Fei; Zhang, Jin-Quan; Xue, Jin-Hua; Wang, Yong-Sheng] Univ S China, Coll Publ Hlth, Hengyang 421001, Peoples R China.;[He, Yan] Univ S China, Coll Life Sci & Technol, Hengyang 421001, Peoples R China.;[Xiao, Xi-Lin; Liang, Jun] Univ S China, Coll Chem & Chem Engn, Hengyang 421001, Peoples R China.
通讯机构：
[Wang, Yong-Sheng] U;Univ S China, Coll Publ Hlth, Hengyang 421001, Peoples R China.
关键词：
Adenosine;Aptamer beacon;Fluorescence-" turning on";Gold nanoparticles;Structure-switching
摘要：
A novel small molecule probe, aptamer beacon (AB), was introduced for adenosine (Ade) recognition and quantitative analysis. The Ade aptamer was engineered into an aptamer beacon by adding a gold nanoparticle-modified nucleotide sequence which is complementary to aptamer sequence (FDNA) at the 3'-end of FDNA. The fluorescence signal "turning on" was observed when AB was bound to Ade, which is attributed to a significant conformational change in AB from a FDNA/QDNA duplex to a FDNA-Ade complex. The Ade measurement was carried out in 20 mmol L-1 Tris-HCl buffer solution of pH 7.4, Delta F signal linearly correlated with the concentration of Ade over the range of 2.0 x 10(-8) to 1.8 x 10(-6) mol L-1. The limit of detection (LOD) for Ade is 6.0 x 10(-9) mol L-1 with relative standard deviations (R.S.D) of 3.64-5.36%, and the recoveries were 98.6%, 100%, 102% (n = 6), respectively. The present method has been successfully applied to determine Ade in human urine samples, and the obtained results were in good agreement with those obtained by the HPLC method. Our investigation shows that the unique properties of the AB could provide a promising potential for small molecules detection, and be benefit to extend the application of aptamer beacon technique. (C) 2012 Elsevier B.V. All rights reserved.
语种：
英文
展开
GC-MS联用技术结合HELP法分析香薷挥发油化学成分
作者：
徐小娜;何小珍;王永生;蒋军辉
期刊：
应用化工 ,2012年41(6):1085-1088,1091 ISSN：1671-3206
作者机构：
[徐小娜; 王永生] 南华大学公共卫生学院;[何小珍] 南华大学药学院;[蒋军辉] 南华大学化学化工学院
关键词：
香薷;挥发油;直观推导式演进特征投影法
摘要：
利用水蒸气蒸馏法提取挥发油,采用GC-MS联用技术结合直观推导式演进特征投影法（HELP）对湖北和江西产香薷的挥发油化学成分进行比较分析。共鉴定出37种化合物,湖北香薷35种,江西香薷19种,分别占总含量的95.16%和96.40%。挥发油主要成分为百里香酚、柠檬烯、β-聚伞花素、百里香酚乙酸酯、香荆芥酚和α-丁香烯。
语种：
中文
展开
Determination of trace formaldehyde in blood plasma by resonance fluorescence technology
作者：
Wang, Yong-Sheng* ;Tan, Xuan;Xue, Jin-Hua;Li, Gui-Rong;Shi, Lin-Fei;...
期刊：
Analytica Chimica Acta ,2011年690(2):234-239 ISSN：0003-2670
通讯作者：
Wang, Yong-Sheng
作者机构：
[Zhou, Bin; Shi, Lin-Fei; Tan, Xuan; Li, Gui-Rong; Xue, Jin-Hua; Yang, Hong-Mei; Wang, Yong-Sheng; Liu, Lu] Univ S China, Coll Publ Hlth, Hengyang 421001, Peoples R China.;[Xiao, Xi-Lin] Univ S China, Coll Chem & Chem Engn, Hengyang 421001, Peoples R China.
通讯机构：
[Wang, Yong-Sheng] U;Univ S China, Coll Publ Hlth, Hengyang 421001, Peoples R China.
关键词：
Blood plasma;Formaldehyde;Potassium bromate;Pyronine Y;Resonance fluorescence
摘要：
A novel method for the determination of trace formaldehyde in blood plasma has been established by using resonance fluorimetry technique. It was based on the fact that oxidation of pyronine Y by potassium bromate was catalyzed by formaldehyde in sulfuric acid. When the wavelength interval was at Δλ=0nm, it was found that the decreased intensity (ΔF) of resonance fluorescence at 574.6nm was proportional to the concentration of formaldehyde in the range of 1.27×10-2 to 2.28μgmL-1. The limit of detection and the average recovery for formaldehyde were 3.80ngmL-1 and 101.6% (n=6), respectively. The present method had been applied to the determination of trace formaldehyde in blood plasma, and the obtained results were in good agreement with those obtained by the resonance light scattering method. © 2011 Elsevier B.V.
语种：
英文
展开
A Wireless magnetoelastic sensor for urinary 2-naphthol detection based on the precipitation of 2-naphthol with diazonium salts
作者：
Yuan, Yu-Kun;Wang, Yong-Sheng* ;Xiao, Xi-Lin;Xue, Jin-Hua;Li, Gui-Rong;...
期刊：
Analytical Sciences ,2011年27(5):517-521 ISSN：0910-6340
通讯作者：
Wang, Yong-Sheng
作者机构：
[Yuan, Yu-Kun; Shi, Lin-Fei; Kang, Rong-Hui; Li, Gui-Rong; Zhang, Jin-Quan; Xue, Jin-Hua; Wang, Yong-Sheng] Univ S China, Coll Publ Hlth, Hengyang 421001, Peoples R China.;[Yuan, Yu-Kun] Chenzhou Fourth Peoples Hosp, Clin Lab, Chenzhou 423000, Peoples R China.;[Xiao, Xi-Lin] Univ S China, Coll Chem & Chem Engn, Hengyang 421001, Peoples R China.
通讯机构：
[Wang, Yong-Sheng] U;Univ S China, Coll Publ Hlth, Hengyang 421001, Peoples R China.
摘要：
A wireless magnetoelastic sensor has been developed for the determination of 2-naphthol (2-NAP) in human urine. This method is based on the precipitation of 2-NAP with diazonium salts produced by the diazo-reaction of sulfamethoxazole (SMZ) with nitrite under a weak alkaline condition, resulting in a descending of the resonance frequency of a wireless magnetoelastic sensor. The frequence shift values (ΔF) of the sensor were directly proportional to the concentration in the range of 1.13 - 139 μmol L -1 for 2-NAP with a correlation coefficient of 0.997 and a detection limit of 0.340 μmol L -1 . The relative standard deviations were 2.38, 2.40 and 2.44%, and the average recovery was 107% (n = 6). The proposed method has additional advantages of being less time-consuming, low cost and remote query, and can be applied for realtime and in situ monitoring of 2-NAP in human urine. It would be a benefit to extend the scope of applications of magnetoelastic sensing techniques. 2011 © The Japan Society for Analytical Chemistry.
语种：
英文
展开
Simultaneous determination of a-naphthol, b-naphthol and 1-hydroxypyrene in urine by synchronous fluorescence spectrometry using b-cyclodextrin as a sensitiser
作者：
Wang, Ying;Wang, Yong-Sheng* ;Yang, Hong-Mei;Xue, Jin-Hua;Tan, Xuan;...
期刊：
International Journal of Environmental Analytical Chemistry ,2011年91(1):87-96 ISSN：0306-7319
通讯作者：
Wang, Yong-Sheng
作者机构：
[Guo, Yu; Wang, Ying; Tan, Xuan; Yang, Hong-Mei; Xue, Jin-Hua; Wang, Yong-Sheng] Univ S China, Coll Publ Hlth, Hengyang 421001, Peoples R China.;[Xiao, Xi-Lin] Univ S China, Coll Chem & Chem Engn, Hengyang 421001, Peoples R China.;[Wang, Ying] Hengyand Blood Ctr, Hengyang 421001, Peoples R China.
通讯机构：
[Wang, Yong-Sheng] U;Univ S China, Coll Publ Hlth, Hengyang 421001, Peoples R China.
关键词：
alpha-naphthol;beta-naphthol;1-hydroxypyrene;synchronous fluorescence;beta-cyclodextrin;double scans;urine
摘要：
A novel method for the simultaneous determination of alpha-naphthol (alpha-NAP), beta-naphthol (beta-NAP) and 1-hydroxypyrene (1-OHP) in human urine has been established by using synchronous fluorescence spectrometry. The measurement was carried out in sodium acetate-boroborax buffer solution (pH = 5.0) with beta-cyclodextrin (beta-CD) enhancing fluorescence. At D Delta lambda = 23 nm, 1-OHP and beta-NAP exhibit maximum signal with minimum interferences from alpha-NAP. At Delta lambda = 175 nm, the signal of alpha-NAP is not influenced by the presence of 1-OHP and beta-NAP. The signals detected at these three wavelengths, 360.2 nm, 330.6nm and 296.4nm, vary linearly when the concentration of 1-OHP, beta-NAP and alpha-NAP is in the range of 0.65-218.3 ng mL(-1), 2.8-1441.0 ng mL(-1) and 3.6-1586.0 ng mL(-1), respectively. The limits of detection (LOD) for alpha-NAP, beta-NAP and 1-OHP were 1.53 ng mL(-1), 0.78 ng mL(-1) and 0.020 ng mL(-1) with relative standard deviations (RSD) of 2.3%, 2.4% and 1.8%, respectively. The proposed method was successfully applied for the simultaneous determination of alpha-NAP, beta-NAP and 1-OHP in human urine samples, and the obtained results were in good agreement with those obtained by the method of high-performance liquid chromatography (HPLC).
语种：
英文
展开
Quartz crystal microbalance with β-cyclodextrin/TiO2 composite films coupled with chemometrics for the simultaneous determination of urinary 1- and 2-naphthol
作者：
Yuan, Yu-Kun;Xiao, Xi-Lin;Wang, Yong-Sheng* ;Xue, Jin-Hua;Li, Gui-Rong;...
期刊：
Sensors and Actuators B-Chemical ,2010年145(1):348-354 ISSN：0925-4005
通讯作者：
Wang, Yong-Sheng
作者机构：
[Yuan, Yu-Kun; Shi, Lin-Fei; Kang, Rong-Hui; Li, Gui-Rong; Zhang, Jin-Quan; Xue, Jin-Hua; Wang, Yong-Sheng] Univ S China, Coll Publ Hlth, Hengyang 421001, Peoples R China.;[Xiao, Xi-Lin] Univ S China, Coll Chem & Chem Engn, Hengyang 421001, Peoples R China.;[Wang, Yong-Sheng] Univ S China, Coll Publ Hlth, W Changsheng Rd 28, Hengyang 421001, Peoples R China.
通讯机构：
[Wang, Yong-Sheng] U;Univ S China, Coll Publ Hlth, W Changsheng Rd 28, Hengyang 421001, Peoples R China.
关键词：
Naphthol;beta-Cyclodextrin;TiO2;Quartz crystal microbalance;Chemometrics
摘要：
A novel sensitive method was presented for the simultaneous determination of 1-naphthol (1-NAP) and 2-naphthol (2-NAP) in human urine samples. This method is based on the combination of the high sensitivity of quartz crystal microbalance (QCM) and excellent predictive ability of chemometrics. Nanocrystalline TiO<inf>2</inf> films were used as substrates for immobilizing β-cyclodextrin (β-CD) onto the QCM surface. The sensing layer consisting of β-CD and nanocrystalline TiO<inf>2</inf> particles was characterized by X-ray photoelectron spectroscopy (XPS), thermogravimetric analysis (TG) and IR spectroscopy. In the Britton-Robinson (B-R) buffer solution of pH 6.37, frequency shift (ΔF) of the QCM was directly proportional to the concentration of 1-NAP and 2-NAP as 1.0 ×10<sup>-6</sup> to 1.3 ×10<sup>-4</sup> mol L<sup>-1</sup>, and 1.0 ×10<sup>-6</sup> to 1.2 ×10<sup>-4</sup> mol L<sup>-1</sup>, respectively. The correlation coefficients were 0.993 for 1-NAP, and 0.990 for 2-NAP. The limits of detection (LOD) were found to be 8.362 ×10<sup>-7</sup> mol L<sup>-1</sup> for 1-NAP and 2.146 ×10<sup>-7</sup> mol L<sup>-1</sup> for 2-NAP. The proposed method has been successfully applied for detecting 1-NAP and 2-NAP in human urine with a satisfactory result. The QCM with β-CD/TiO<inf>2</inf> composite films coupled with chemometrics can lead to significant improvements in the sensor performance, and would be benefit to extend the scope of application of QCM and chemometrics. ©2009 Elsevier B.V. All rights reserved.
语种：
英文
展开
Rapid simultaneous analysis of 1-hydroxypyrene, 2-hydroxyfluorene, 9-hydroxyphenanthrene, 1- and 2-naphthol in urine by first derivative synchronous fluorescence spectrometry using Tween-20 as a sensitizer
作者：
Kang, Rong-Hui;Wang, Yong-Sheng* ;Yang, Hong-Mei;Li, Gui-Rong;Tan, Man;...
期刊：
Analytica Chimica Acta ,2010年658(2):180-186 ISSN：0003-2670
通讯作者：
Wang, Yong-Sheng
作者机构：
[Yuan, Yu-Kun; Tan, Man; Shi, Lin-Fei; Kang, Rong-Hui; Li, Gui-Rong; Zhang, Jin-Quan; Yang, Hong-Mei; Xue, Jin-Hua; Wang, Yong-Sheng] Univ S China, Coll Publ Hlth, Hengyang 421001, Peoples R China.;[Xiao, Xi-Lin] Univ S China, Coll Chem & Chem Engn, Hengyang 421001, Peoples R China.
通讯机构：
[Wang, Yong-Sheng] U;Univ S China, Coll Publ Hlth, Hengyang 421001, Peoples R China.
关键词：
Derivative synchronous fluorescence;Polycyclic aromatic hydrocarbons;Tween-20;Urine
摘要：
A novel method of first derivative synchronous fluorescence was developed for the rapid simultaneous analysis of trace 1-hydroxypyrene (1-OHP), 1-naphthol (1-NAP), 2-naphthol (2-NAP), 9-hydroxyphenanthrene (9-OHPe) and 2-hydroxyfluorene (2-OHFlu) in human urine. Only one single scan was needed for quantitative determination of five compounds simultaneously when Δλ = 10 nm was chosen. In the optimal experimental conditions, there was a linear relationship between the fluorescence intensity and the concentration of 1-OHP, 1-NAP, 2-NAP, 9-OHPe and 2-OHFlu in the range of 1.75 × 10-9 to 4.50 × 10-6 mol L-1, 3.64 × 10-8 to 2.20 × 10-4 mol L-1, 8.18 × 10-9 to 1.20 × 10-4 mol L-1, 3.26 × 10-9 to 8.50 × 10-5 mol L-1 and 4.88 × 10-9 to 5.50 × 10-6 mol L-1, respectively. The limits of detection (LOD) were found to be 5.25 × 10-10 mol L-1 for 1-OHP, 1.10 × 10-8 mol L-1 for 1-NAP, 2.46 × 10-9 mol L-1 for 2-NAP, 9.77 × 10-10 mol L-1 for 9-OHPe and 1.46 × 10-9 mol L-1 for 2-OHFlu. The proposed method is reliable, selective and sensitive, and has been used successfully in the determination of traces of 1-OHP, 1-NAP, 2-NAP, 9-OHPe and 2-OHFlu in human urine samples, whose results were in good agreement with those gained by the HPLC method. © 2009 Elsevier B.V. All rights reserved.
语种：
英文
展开
A catalytic resonance fluorometry method for determination of hydroquinone and its applications
作者：
Li, Guirong* ;Tan, Guanghui;Liu, Yunfu;Wang, Yongsheng;Liao, Lifu
期刊：
Analytical Sciences ,2009年25(4):493-497 ISSN：0910-6340
通讯作者：
Li, Guirong
作者机构：
[Wang, Yongsheng; Tan, Guanghui; Liu, Yunfu; Li, Guirong] Univ S China, Coll Publ Hlth, Hengyang 421001, Peoples R China.;[Liao, Lifu] Univ S China, Coll Chem & Chem Engn, Hengyang 421001, Peoples R China.
通讯机构：
[Li, Guirong] U;Univ S China, Coll Publ Hlth, Hengyang 421001, Peoples R China.
摘要：
Pyronin Y (PY) has a strong resonance fluorescence in sulfuric acid medium. The characteristics of the resonance fluorescence spectra and the factors affecting the spectra were studied. A catalytic resonance fluorometry method for the determination of hydroquinone was proposed based on the catalytic effect of hydroquinone on the oxidation of PY by potassium bromate. The oxidation of PY resulted in the decrease of the resonance fluorescence intensity. The influences of several variables on the sensitivity were studied. At the optimized conditions, the decrease of the resonance fluorescence intensity was in proportion to the concentration of hydroquinone in the range of 4.42 - 1.60 × 103 μg l-1, and the detection limit was 1.46 μg l-1. The proposed method was applied successfully for the determination of trace hydroquinone in environment samples. The relative standard deviation was less than 3.90% and the recoveries were in the range of 95.2 - 104.0%. 2009 © The Japan Society for Analytical Chemistry.
语种：
英文
展开
Pb-XO配合物显色光度法测定水样中微量铅
作者：
肖锡林;魏永卷;薛金花;袁玉坤;王永生
期刊：
应用化工 ,2009年38(2):296-299 ISSN：1671-3206
作者机构：
[肖锡林; 魏永卷; 薛金花; 袁玉坤; 王永生] 南华大学,公共卫生学院化学化工学院
关键词：
分光光度法;二甲酚橙;铅
摘要：
二甲酚橙是微量分析中一种很重要的光度显色剂,在pH 6.0的磷酸-醋酸-硼酸缓冲溶液中,二甲酚橙与铅形成稳定的1∶1红色配合物,导致吸光度的增强.研究了铅与三苯甲烷类染料二甲酚橙的显色反应体系,发现在波长580 nm处吸光度的增强值与铅的加入量有很好的线性关系,据此建立了测定微量铅的新方法.该方法的摩尔吸光数为3.312×10~4 L/(mol · cm),铅离子的浓度在0.075～1.2 mg/L范围内符合比耳定律.方法简便、灵敏、选择性好,可广泛应用.
语种：
中文
展开
茜素红催化光度法测定人尿中痕量萘酚
作者：
肖锡林;段艳红;杨红梅;袁玉坤;王永生
期刊：
分析仪器 ,2009年(3):29-32 ISSN：1001-232X
作者机构：
南华大学公共卫生学院,化学化工学院,衡阳,421001;[杨红梅; 肖锡林; 王永生; 袁玉坤; 段艳红] 南华大学
关键词：
催化;分光光度法;茜素红;萘酚
摘要：
根据在稀硫酸溶液中,萘酚能催化溴酸钾氧化茜素红褪色的反应,且萘酚浓度与ΔA呈线性关系,建立了催化褪色光度法测定萘酚的新方法.考察了体系ΔA的影响因素,优化了反应条件.最大吸收峰位于422.0 nm波长处,α-萘酚的浓度在2.93～23.0 μg.L-1范围内与ΔA呈良好的线性关系,方法检出限为0.88 μg.L-1.已用于人尿样品中测定,测定结果令人满意.
语种：
中文
展开
The molecular resonance fluorescence method for determination of arsenic in hair samples
作者：
Li, Guirong* ;Liu, Yunfu;Wang, Yongsheng;Liao, Lifu
期刊：
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY ,2009年72(4):811-815 ISSN：1386-1425
通讯作者：
Li, Guirong
作者机构：
[Wang, Yongsheng; Liu, Yunfu; Li, Guirong] Univ S China, Coll Publ Hlth, Hengyang 421001, Peoples R China.;[Liao, Lifu] Univ S China, Coll Chem & Chem Engn, Hengyang 421001, Peoples R China.
通讯机构：
[Li, Guirong] U;Univ S China, Coll Publ Hlth, Hengyang 421001, Peoples R China.
关键词：
Molecular resonance fluorescence;Arsenic;Rhodamine B;Hair samples
摘要：
A molecular resonance fluorescence quenching method for the determination of arsenic was proposed. The method is based on the quenching effect of As (V) on the molecular resonance fluorescence of Rhodamine B (RhB) in sulfuric acid medium and in the presence of molybdate and PVA. The influences of acidity, chemical and manifold variables on the sensitivity were studied. At the optimized conditions, the linear range for the determination of arsenic was 2.0 ×10<sup>-3</sup>-0.12 μg ml<sup>-1</sup> with the detection limit of 1.20 ×10<sup>-9</sup> g ml<sup>-1</sup>. The relative standard deviations for the eleven replicate determinations of 0.006 μg ml<sup>-1</sup> and 0.060 μg ml<sup>-1</sup> of arsenic were 1.12% and 1.61%, respectively. The proposed method was applied successfully for the determination of trace arsenic in hair samples with the recoveries of 94.8-104.6%. ©2008 Elsevier B.V. All rights reserved.
语种：
英文
展开
