A label-free ultrasensitive and selective strategy for Pb(ii) assay by a multifunctional DNA probe-mediated rolling-circle amplified synthesis of the G-quadruplexes
National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [21177052, 81502850]; Natural Science Foundation of Hunan Province in ChinaNatural Science Foundation of Hunan Province [2015JJ2122]; Science and Technology Program of Hunan Province in China [2010SK3039]
机构署名：
本校为第一且通讯机构
院系归属：
化学化工学院
公共卫生学院
摘要：
We report a label-free ultrasensitive and selective strategy for Pb(ii) assay by a multifunctional DNA probe (MDP)-mediated rolling-circle amplified synthesis of the G-quadruplexes. The MDP acted as a target recognition probe, a catalytic DNAzyme and a primer of rolling-circle amplification (RCA). The presence of Pb(ii) can induce the conformational switching of the MDP, resulting in the cleavage of the S-DNA in the MDP to release an E-DNA. The released E-DNA then initiated a RCA reaction with a reasonably devised padlock DNA template to produc...
