Inducible nitric oxide synthase and cyclooxgenase-2 mediate protection of hydrogen peroxide preconditioning against apoptosis induced by oxidative stress in PC12 cells
作者：
Tang, Xiao-qing;Yu, Hui-min;Zhi, Jun-li;Cui, Yu;Tang, Er-hu;...
期刊：
Life Sciences ,2006年79(9):870-876 ISSN：0024-3205
通讯作者：
Feng, Jian-qiang
作者机构：
[Feng, Jian-qiang] Sun Yat Sen Univ, Zhongshan Med Coll, Dept Physiol, Guangzhou 510080, Peoples R China.;Nahua Univ, Coll Med, Dept Physiol, Hengyang City 421001, Hunan, Peoples R China.
通讯机构：
[Feng, Jian-qiang] S;Sun Yat Sen Univ, Zhongshan Med Coll, Dept Physiol, Guangzhou 510080, Peoples R China.
关键词：
Inducible nitric oxide synthase;Cyclooxygenase;Hydrogen superoxide;Apoptosis;Preconditioning
摘要：
The induction of inducible nitric oxide synthase (iNOS) in response to different stress is associated with simultaneous induction of cyclooxygenase-2 (COX-2) in various cell types. Both iNOS and COX-2 have been reported to mediate the late phase of cardioprotection induced by different preconditioning. However, whether both iNOS and COX-2 are mediators in the neuroprotection induced by preconditioning with hydrogen peroxide (H(2)O(2)) at low concentration is unknown. In this study, using the neurosecretory cell line-PC12 cells to set up the model of neuroprotection of preconditioning with H(2)O(2) against apoptosis, we first investigate what changes in expression of iNOS and COX-2 appear during H(2)O(2) preconditioning, then determine if both iNOS inhibitor and COX-2 inhibitor interfere with the neuroprotection elicited by preconditioning with H(2)O(2). We found that preconditioning with H(2)O(2) at 10 microM significantly protected PC12 cells against apoptosis induced by lethal H(2)O(2) (50 microM) and increased the expression of iNOS and COX-2 and that selective iNOS inhibitor, aminoguanidine (AG) and COX-2 inhibitor, NS-398 obviously blocked the protective effects induced by preconditioning with 10 microM H(2)O(2). The results of this study suggest that both iNOS and COX-2 are mediators of the neuroprotection induced by preconditioning with oxidative stress (H(2)O(2) at low concentration) in PC12 cells.
语种：
英文
展开
小型猪动脉粥样硬化斑块稳定性模型研究
作者：
Liu, LS;Yang, YZ* ;Feng, DM;Sun, WQ;Tang, CK;...
期刊：
生物化学与生物物理进展 ,2006年33(2):196-200 ISSN：1000-3282
通讯作者：
Yang, YZ
作者机构：
[Wan, ZY; Wang, Z; Sun, WQ; Ren, Z; Tang, CK; Liu, LS; Feng, DM; Tang, YL; Yang, YZ; Yang, BT; Yi, GH; Wang, GX] Nanhua Univ, Inst Cardiovasc Dis, Hengyang 421001, Peoples R China.
通讯机构：
[Yang, YZ] N;Nanhua Univ, Inst Cardiovasc Dis, Hengyang 421001, Peoples R China.
关键词：
动脉粥样硬化;斑块破裂;猪;动物模型
摘要：
目前已有的动物模型在研究动脉粥样硬化斑块破裂、破裂的可控性及量化研究方面均不能满足研究的需要．为了建立类似于人类动脉粥样硬化病变的斑块模型，体外研究斑块稳定性，应用传统的高脂高胆固醇膳食诱导建立了小型猪动脉粥样硬化模型，并从血脂水平和斑块病理形态学特征方面加以了证实．该模型中斑块与人类成熟斑块的高度相似性使其成为研究斑块稳定性和斑块破裂的较好模型．从量化比较这一出发点着手，建立了一个体外可控可量化诱导斑块破裂模型，方法简单易行，是一个较好的量化研究斑块破裂和破裂相关因素间关系的实验模型。
语种：
中文
展开
Aggravation of atherosclerosis by mast cells in the New Zealand white rabbit with stegnotic carotid artery
作者：
Sun, W. Q.;Yang, Y. Z.;Ye, X.;Tang, Y. L.;Peng, K.;...
期刊：
Atherosclerosis Supplements ,2006年7(3):251-251 ISSN：1567-5688
作者机构：
[Peng, K.; Sun, Y. H.; Sun, W. Q.; Dong, B. H.; Jin, H. Y.; Tang, Y. L.; Yang, Y. Z.; Ye, X.] Nanhua Univ, Inst Cardiovasc Dis, Hengyang, Peoples R China.
语种：
英文
展开
Th-P15:209 Mast cells change cellular cholesterol contents and inhibit cholesterol efflux from THP-1 macrophage-derived foam cells
作者：
Zhao, Z. Z.;Yang, Y. Z.;Wang, S.;Dong, B. H.;Wang, Z.;...
期刊：
Atherosclerosis Supplements ,2006年7(3):539-539 ISSN：1567-5688
作者机构：
[Wei, D. H.; Wang, Z.; Dong, B. H.; Zhao, Z. Z.; Liu, L. S.; Yang, Y. Z.; Wang, S.] Nanhua Univ, Inst Cardiovasc Dis, Hengyang, Peoples R China.
语种：
英文
展开
Effect of liver X receptor agonist on Niemann-Pick C-1 expression and intracellular cholesterol transportation in lipid-loaded THP-1 macrophages
作者：
Ou, X.;Tang, C. K.;Dai, X. Y.;Yang, J. H.;Hao, X. R.;...
期刊：
Atherosclerosis Supplements ,2006年7(3):492-492 ISSN：1567-5688
作者机构：
[Ou, X.; Wang, Z.; Hao, X. R.; Cao, D. L.; Tang, C. K.; Dai, X. Y.; Yang, J. H.; Wang, Z. Q.; Jiang, Z. S.; Yi, G. H.] Nanhua Univ, Inst Cardiovasc Dis, Hengyang, Peoples R China.
语种：
英文
展开
Mo-P6:445 Polymorphism of-191C/G of ABCA1 gene in patients with coronary artery disease in hans population
作者：
Shi, W. Y.;Yang, Y. Z.;Quan, Z. H.;Tang, C. K.;Liu, C. H.;...
期刊：
Atherosclerosis Supplements ,2006年7(3):144-144 ISSN：1567-5688
作者机构：
[Ouyang, G.; Shi, W. Y.; Quan, Z. H.; Li, Y. Q.; Tang, C. K.; Li, H. B.; Liu, C. H.; Yang, Y. Z.; Yang, X.] Nanhua Univ, Inst Cardiovasc Dis, Hengyang, Peoples R China.
语种：
英文
展开
Vascular Basement Membrane-derived Multifunctional Peptide, a Novel Inhibitor of Angiogenesis and Tumor Growth
作者：
Cao, Jian-Guo;Peng, Shu-Ping;Sun, Li;Li, Hui;Wang, Li;...
期刊：
生物化学与生物物理学报 ,2006年38(7):514-521 ISSN：1672-9145
通讯作者：
Deng, Han-Wu
作者机构：
[Deng, Han-Wu] Cent S Univ, Sch Pharmaceut Sci, Dept Pharmacol, Changsha 410078, Peoples R China.;Nanhua Univ, Canc Res Inst, Hengyang 421001, Peoples R China.
通讯机构：
[Deng, Han-Wu] C;Cent S Univ, Sch Pharmaceut Sci, Dept Pharmacol, Changsha 410078, Peoples R China.
关键词：
recombinant vascular basement membrane-derived multifunctional peptide;angiogenesis inhibitor;therapeutic action
摘要：
Vascular basement membrane-derived multifunctional peptide (VBMDMP) gene (fusion gene of the human immunoglobulin G3 upper hinge region and two tumstatin-derived fragments) obtained by chemical synthesis was cloned into vector pUC19, and introduced into the expression vector pGEX-4T-1 to construct a prokaryotic expression vector pGEX-4T-1-VBMDMP. Recombinant VBMDMP produced in Escherichia coli has been shown to have significant activity of antitumor growth and antimetastasis in Lewis lung carcinoma transplanted into mouse C57Bl/6. In the present study, we have studied the ability of rVBMDMP to inhibit endothelial cell tube formation and proliferation, to induce apoptosis in vitro, and to suppress tumor growth in vivo. The experimental results showed that rVBMDMP potently inhibited proliferation of human endothelial (HUVEC-12) cells and human colon cancer (SW480) cells in vitro, with no inhibition of proliferation in Chinese hamster ovary (CHO-K1) cells. rVBMDMP also significantly inhibited human endothelial cell tube formation and suppressed tumor growth of SW480 cells in a mouse xenograft model. These results suggest that rVBMDMP is a powerful therapeutic agent for suppressing angiogenesis and tumor growth. ©Institute of Biochemistry and Cell Biology, SIBS, CAS.
语种：
英文
展开
Tu-P7:175 Effects of high level expression of NADPH oxidase 4 on apoptosis of endothelial cells
作者：
Qu, S. L.;Sun, W. Q.;Wang, S.;Guo, F.;Yang, X. D.;...
期刊：
Atherosclerosis Supplements ,2006年7(3):223-223 ISSN：1567-5688
作者机构：
Minist Publ Hlth, Inst Geriatr Med, Beijing, Peoples R China.;Nanhua Univ, Dept Med Sch, Hunan, Peoples R China.
语种：
英文
展开
Apolipoprotein A-1 activates ABCA1 gene transcription and protein synthesis
作者：
Yang, J. H.;Tang, C. K.;Dai, X. Y.;Ou, X.;Wang, Z. Q.;...
期刊：
Atherosclerosis Supplements ,2006年7(3):543-543 ISSN：1567-5688
作者机构：
[Ou, X.; Wang, Z.; Hao, X. R.; Cao, D. L.; Tang, C. K.; Dai, X. Y.; Yang, J. H.; Wang, Z. Q.; Jiang, Z. S.; Yi, G. H.] Nanhua Univ, Inst Cardiovasc Dis, Hengyang, Peoples R China.
语种：
英文
展开
Th-P15:11 The role of PPARs in cellular cholesterol efflux mediated by high density lipoprotein. A possible involvement of ABCA1, LXRALPHA, and ERK1/2
作者：
Yi, G. -H.;Yin, X. -B.;Tan, Y. L.;Zeng, Y.;Feng, J. -T.;...
期刊：
Atherosclerosis Supplements ,2006年7(3):494-494 ISSN：1567-5688
作者机构：
[Wang, J.; Ren, Z.; Yin, X. -B.; Zeng, Y.; Zeng, D. -X.; Sun, L.; Feng, J. -T.; Tan, Y. L.; Yi, G. -H.; Xia, J. -H.] Nanhua Univ, Inst Cardiovasc Dis, Hengyang, Peoples R China.
语种：
英文
展开
Small interfering RNA targeting the Bcl-2 gene induced apoptosis of HL-60 cell
作者：
Yan, Chun-yan;Lei, Xiao-yong;Zhong, Miao;Feng, Lan-fang;Tu, Yu-lin;...
期刊：
中国药理学报 ,2006年27:411-411 ISSN：1671-4083
作者机构：
Nanhua Univ, Inst Pharm & Pharmacol, Hengyang 421001, Peoples R China.;Nanhua Univ, Inst Cardiovasc Dis, Hengyang 421001, Peoples R China.
关键词：
small interfering RNA;Bcl-2;apoptosis;HL-60
语种：
英文
展开
Effects of rosiglitazone on collagen I/III and secretion of TGF beta 1 of vascular smooth muscle cell induced by high glucose
作者：
Wang, Bing-Xiang;Ling, Hong-Yan;Zhang, Yu-Chang;Feng, Shui-Dong;Hu, Bi
期刊：
中国药理学报 ,2006年27:162-162 ISSN：1671-4083
作者机构：
Nanhua Univ, Dept Physiol, Hengyang 421001, Peoples R China.;Nanhua Univ, Dept Epidemiol, Hengyang 421001, Peoples R China.
关键词：
rosiglitazone;high glucose;VSMC;collagen I / III;TGF beta 1
语种：
英文
展开
Diallyl disulfide suppresses growth of HL-60 cell through increasing histone acetylation and p21WAF1 expression in vivo and in vitro
作者：
Zhao, Jie;Huang, Wei-guo;He, Jie;Tan, Hui;Liao, Qian-jin;...
期刊：
中国药理学报 ,2006年27(11):1459-1466 ISSN：1671-4083
通讯作者：
Su, Qi
作者机构：
[Su, Qi] Nanhua Univ, Inst Canc Res, Hengyang 421001, Peoples R China.;Mawangdui Hosp, Dept Pathol, Changsha 410016, Peoples R China.
通讯机构：
[Su, Qi] N;Nanhua Univ, Inst Canc Res, Hengyang 421001, Peoples R China.
关键词：
diallyl disulfide;leukemia;growth inhibition;histone acetylation;p21WAF1
摘要：
Aim: To examine the differentiation induction and growth inhibition of HL-60 cells by diallyl disulfide (DADS), and its relationship with the alterations of histone acetylation and p21WAF1 expression in vitro and in vivo. Methods: Differentiation was studied by nitroblue tetrazolium (NBT) reduction of HL-60 cell in vitro. HL-60 cells 5×106 were injected into the right side of the peritoneal cavity of severe combined immunodeficiency (SCID) mice. When the peritoneal neoplasms were detected, the SCID mice were randomly divided into 3 groups and received an ip injection of vehicle alone (NS), DADS or sodium butyrate (SB). The growth inhibition of peritoneal neoplasms induced by DADS was observed by a growth curve. The cycle distribution of HL-60 cells in SCID mice was monitored by flow cytometry. The expression of acetylated histone H3, H4 and p21WAF1 were measured by Western blot. Results: After treatment with DADS for 0-72 h, the NBT reduction ability of HL-60 cells increased in a time-dependent manner, compared with no treatment of HL-60 cells. In the HL-60 cells treated with DADS for 24 h, the expression of acetylated histone H3, H4, and p21WAF1 increased obviously. After treatment with DADS, tumor growth was markedly suppressed. HL-60 cells from mice treated with DADS were blocked in the G1 phase, from 25.4% to 63.4%. The tumors from the mice treated with DADS showed an increase of acetylated histone H3, H4, and p21WAF1. Conclusion: DADS could induce differentiation and inhibit the growth of HL-60 cells through increasing the expression of acetylated histone H3, H4, and p21WAF1 in vitro and in vivo. © 2006 CPS and SIMM.
语种：
英文
展开
Effects of NO-1886 on inflammation-associated cytokines in atherosclerosis associated diabetes mellitus miniature pigs
作者：
Cai, M. B.;Li, J. J.;Yin, W. D.;Liao, D. F.;Li, Q. K.;...
期刊：
Atherosclerosis Supplements ,2006年7(3):195-195 ISSN：1567-5688
作者机构：
Nanhua Univ, Sch Life Sci & Technol, Dept Biochem & Mol Biol, Hengyang, Hunan, Peoples R China.;Nanhua Univ, Affiliated Hosp 2, Dept Renal Transplantat, Hengyang, Hunan, Peoples R China.;Nanhua Univ, Inst Cardiovasc Res, Hengyang, Hunan, Peoples R China.;Nanhua Univ, Sch Life Sci & Technol, Dept Lab Anim Sci, Hengyang, Hunan, Peoples R China.
语种：
英文
展开
Tu-P7:199 NO-1886 (IBROLIPIM) reduced plasma triglycerides and free fatty acids in fat-loaded Bama minipigs
作者：
Hou, H. J.;Yin, W. D.;Li, Q. K.;Cai, M. B.;Zhang, C.;...
期刊：
Atherosclerosis Supplements ,2006年7(3):228-228 ISSN：1567-5688
作者机构：
Nanhua Univ, Sch Life Sci & Technol, Hengyang, Hunan, Peoples R China.;Nanhua Univ, Inst Cardiovasc Dis, Hengyang, Hunan, Peoples R China.;Nanhua Univ, Inst Tumor Researcher, Hengyang, Hunan, Peoples R China.
语种：
英文
展开
Tu-P7:219 Oxidized low density lipoprotein up-regulate PCSK9 expression in rat vascular smooth muscle cells in vitro
作者：
Liu, L. S.;Cheng, Y. L.;Xie, M.;Tang, C. K.;Wang, Z.;...
期刊：
Atherosclerosis Supplements ,2006年7(3):233-233 ISSN：1567-5688
作者机构：
Nanhua Univ, Inst Cardiovasc Dis, Heng Yang, Hunan, Peoples R China.;Chongqing Univ, Bioengn Coll, Chongqing 630044, Peoples R China.
语种：
英文
展开
The centrally cardiovascular regulative effect of adrenomedullin in the medulla oblongata on stress-induced hypertensive rats
作者：
Xia, Li;Liang, Li;Yi, Wang;Jin, Wang;Lin, Shen Lin;...
期刊：
JOURNAL OF HYPERTENSION ,2006年24:308-308 ISSN：0263-6352
作者机构：
ESPCI, CNRS, UMR 7637, Lad Neurobiol, F-75005 Paris, France.;Fudan Univ, Shanghai Med Coll, Dept Physiol & Phatholphysiol, Shanghai 200433, Peoples R China.;Nanhua Univ, Nanhua Med Coll, Dept Physiol, Hunan, Peoples R China.;Fudan Univ, Shanghai Med Coll, Dept Phatholphysiol, Shanghai 200433, Peoples R China.
语种：
英文
展开
NO-1886 upregulates ATP binding cassette transporter A1 and inhibits diet-induced atherosclerosis in Chinese Bama minipigs
作者：
Zhang, Chi;Yin, Weidong* ;Liao, Duanfang;Huang, Liang;Tang, Chaoke;...
期刊：
Journal of Lipid Research ,2006年47(9):2055-2063 ISSN：0022-2275
通讯作者：
Yin, Weidong
作者机构：
[Yin, Weidong] Nanhua Univ, Sch Med, Inst Cardiovasc Res, Hengyang 421001, Hunan, Peoples R China.;Nanhua Univ, Sch Life Sci & Technol, Dept Biochem & Mol Biol, Hengyang 421001, Hunan, Peoples R China.;Nanhua Univ, Sch Med, Funct Lab Ctr, Hengyang 421001, Hunan, Peoples R China.;Nanhua Univ, Inst Pharm & Pharmacol, Hengyang 421001, Hunan, Peoples R China.;Nanhua Univ, Sch Med, Dept Operat Surg, Hengyang 421001, Hunan, Peoples R China.
通讯机构：
[Yin, Weidong] N;Nanhua Univ, Sch Med, Inst Cardiovasc Res, Hengyang 421001, Hunan, Peoples R China.
关键词：
liver X receptor alpha;scavenger receptor class B type I;[4-(4-bromo-2-cyano-phenylcarbamoyl)-benzyl] phosphonic;acid diethyl ester
摘要：
It is widely believed that high density lipoprotein-cholesterol (HDL-C) functions to transport cholesterol from peripheral cells to the liver by reverse cholesterol transport (RCT), a pathway that may protect against atherosclerosis by clearing excess cholesterol from arterial cells. A cellular ATP binding cassette transporter called ABCA1 mediates the first step of RCT. NO-1886 has been proven to be highly effective at increasing HDL-C and reducing atherosclerosis. However, the mechanism of atherosclerosis inhibition for NO-1886 is not fully understood. In this study, the effects of NO-1886 on ABCA1 were investigated in high-fat/high-sucrose/high-cholesterol-fed Chinese Bama minipigs. Administration of NO-1886 (0.1 g/kg body weight/day) in the diet for 5 months significantly reduced atherosclerosis lesions and significantly increased plasma HDL-C and apolipoprotein A-I levels. The mRNA and protein levels of ABCA1 in the liver, retroperitoneal adipose tissue, and aorta were increased by NO-1886 as well. Multivariate linear regression analysis showed that the levels of LPL in plasma and the levels of ABCA1 in aorta were independently associated with the atherosclerotic lesion area. In addition, NO-1886 upregulated liver X receptor α and affected the expression of scavenger receptor class B type I in the liver. These results demonstrate that the mechanism of atherosclerosis inhibition for NO-1886 is associated with its effect on ABCA1. Copyright ©2006 by the American Society for Biochemistry and Molecular Biology, Inc.
语种：
英文
展开
Effects of high concentration of glucose, low density lipoprotein and angiotensin medium on NADPH oxidase 4 and apoptosis of endothelial cells
作者：
Qu, S. L.;Wang, S.;Gu, F.;Yang, X. D.;Li, J.
期刊：
Atherosclerosis Supplements ,2006年7(3):293-293 ISSN：1567-5688
作者机构：
Minist Publ Hlth, Inst Geriatr Med, Beijing, Peoples R China.;Nanhua Univ, Dept Med Sch, Hengyang, Hunan, Peoples R China.
语种：
英文
展开
We-W38:7 NO-1886 suppressing atherosclerosis in high-fat/high-sucrose/high-cholesterol fed Bama minipigs is related to upregulating ABCA1
作者：
Zhang, C.;Yin, W. D.;Liao, D. F.;Huang, L.;Wang, Z. B.;...
期刊：
Atherosclerosis Supplements ,2006年7(3):322-322 ISSN：1567-5688
作者机构：
Nanhua Univ, Sch Med, Cardiovasc Res Inst, Hengyang, Hunan, Peoples R China.;Nanhua Univ, Sch Life Sci & Technol, Dept Biochem & Biotechnol, Hengyang, Hunan, Peoples R China.;Nanhua Univ, Sch Med, Funct Lab Ctr, Hengyang, Hunan, Peoples R China.;Otsuka Pharmaceut Factory Inc, Res & Dev, Tokushima, Japan.
语种：
英文
展开
