作者机构：
[Hao, Xinrui; Ou, Xiang; Tang, Yaling; Hu, Yanwei; Li, Xiaoxu; Tang, Chaoke; Cao, Dongli; Dai, Xiaoyan] Univ S China, Key Lab Atherosclerol Human Prov, Cardiovasc Res Inst, Hengyang 421001, Peoples R China.
通讯机构：
[Tang, Chaoke] U;Univ S China, Key Lab Atherosclerol Human Prov, Cardiovasc Res Inst, Hengyang 421001, Peoples R China.
关键词：
semicarbazide-sensitive amine oxidase;nuclear receptor;liver X receptor;T0901317;atherosclerosis
摘要：
Semicarbazide-sensitive amine oxidase (SSAO) catalyzes oxidative deamination of primary aromatic and aliphatic amines. Increased SSAO activity has been found in atherosclerosis and diabetes mellitus. We hypothesize that the anti-atherogenic effect of liver X receptors (LXRs) might be related to the inhibition of SSAO gene expression and its activity. In this study, we investigated the effect of LXRagonist T0901317 on SSAO gene expression and its activity in apolipoprotein E knockout (apoE−/−) mice. Male apoE−/− mice (8 weeks old) were randomly divided into four groups: basal control group; vehicle group; prevention group; and treatment group. SSAO gene expression was analyzed by real-time quantitative polymerase chain reaction and its activity was determined. The activity of superoxide dismutase and content of malondialdehyde in the aorta and liver were also determined. In T0901317-treated mice, SSAO gene expression was significantly decreased in the aorta, liver, small intestine, and brain. SSAO activities in serum and in these tissues were also inhibited. The amount of superoxide dismutase in the aorta and liver of the prevention group and treatment group was significantly higher compared with the vehicle group (P < 0.05). Malondialdehyde in the tissues of these two groups was significantly lower compared with the vehicle group (P < 0.05). Our results showed that T0901317 inhibits SSAO gene expression and its activity in atherogenic apoE−/− mice. The atheroprotective effect of LXR agonist T0901317 is related to the inhibition of SSAO gene expression and its activity.
摘要：
CD40L and B lymphocyte-activating factor (BAFF), members of the TNF superfamily, play critical roles in B cell survival and activation, and in the regulation of humoral immunity. We previously reported that the adaptor molecule Act1 functions as a negative regulator of CD40- and BAFF-mediated B cell survival. Here we demonstrated that mice deficient in Act1 developed systemic autoimmune disease with histological and serological features of human Sjogren's syndrome (SS), in association with systemic lupus erythematosus-like nephritis. Analyses of Act1(-/-)CD40(-/-) and Act1(-/-)BAFF(-/-) double-deficient mice revealed that Act1 regulates different stages of the disease development through its impact on both CD40- and BAFF-mediated pathways. We found that Act1 modulates the survival of autoreactive B cells mainly through its negative regulatory role in BAFF-mediated cell survival, while the effect of Act1 on autoantibody production is likely through modulation of CD40-mediated T cell-dependent antibody response. The impact of Act1 on both BAFF and CD40 pathways establishes Act1-deficient mice as a unique model to study distinct steps of autoimmunity and regulation of self tolerance.
摘要：
Background: Scavenger receptor that binds phosphatidylserine and oxidized lipoprotein/CXC chemokine ligand 16 (SR-PSOX/CXCL 16) promotes foam cell formation through the tumor necrosis factor (TNF)-α mediated mechanism. Because chemokine CXCL16 could be expressed in atherosclerotic lesions and induce smooth muscle, cell (SMC) proliferation, we presume that the monocyte SR-PSOX/CXCL16 detection in the patients peripheral blood will be important for early diagnosis and prognosis of atherosclerosis (AS). Methods: Enrolled in this study were 40 patients with acute coronary syndrome (ACS), including 20 patients with acute myocardial infarction (AMI) and 20 patients with unstable angina pectoris (UAP), and 20 normal controls. Monocytes in the peripheral blood were isolated, and the changes of expression of CXCL16/SR-PSOX mRNA were compared using reverse transcription-polymerase chain reaction (RT-PCR), with β-actin as internal control. We compared the expression of CXCL16/SR-PSOX in the ACS subgroups, using Western-blot to analyze protein expression levels. Tissue sections were made from biopsy specimens taken from patients with infective endocarditis, liver cirrhosis, and lung cancer as well as normal controls. And the expression of CXCL16/SR- PSOX was analyzed with a confocal microscope. Results: The expression of CXCL16/SR-PSOX mRNA and protein in the monocytes of peripheral blood was significantly higher in ACS patients than in normal controls (P<0.05); however, there was no significant difference in CXCL16/SR-PSOX expression between UAP group and AMI group (P>0.05). Immunofluorescence showed that there were low expression of SR-PSOX in normal vascular endothelial cells and enhanced expression in every layer of the infected vessels, while spreading from endothelial cells to surrounding tissues as infection worsens. Confocal microscopy showed that the expression of SR-PSOX was enhanced in the infiltrated lymphocytes in liver cirrhosis, and that the expression level was proportionate to the degree of inflammation in the portal hepatis and folia. Conclusions: The expression of CXCL16/SR-PSOX in the monocytes of peripheral blood was significantly higher in ACS patients than in the controls. CXCL16/SR-PSOX-mediated inflammation may contribute to the pathogenesis of ACS, and CXCL16 may play an important role in the pathogenesis and development of AS in humans.
期刊：
Journal of Neuroscience Methods,2008年170(1):111-116 ISSN：0165-0270
通讯作者：
Hu, Xin-Tian
作者机构：
[Fraser, Wilson A. W.; Meng, Zhi-Qiang; Wang, Jian-Hong; Hu, Xin-Tian; Zhang, Bo; Sun, Ning-Lei] Chinese Acad Sci, Kunming Inst Zool, Lab Sensory Motor Integrat Res, Kunming 650223, Yunnan, Peoples R China.;[Fraser, Wilson A. W.; Meng, Zhi-Qiang; Wang, Jian-Hong; Hu, Xin-Tian; Zhang, Bo; Ma, Yuan-Ye; Sun, Ning-Lei] Chinese Acad Sci, Kunming Inst Zool, Key Lab Anim Models & Human Dis Mech, Kunming 650223, Yunnan, Peoples R China.;[Fraser, Wilson A. W.; Meng, Zhi-Qiang; Wang, Jian-Hong; Zhang, Bo; Ma, Yuan-Ye; Sun, Ning-Lei] Chinese Acad Sci, Kunming Inst Zool, Sect Primate Neurosci Res Lab, Kunming 650223, Yunnan, Peoples R China.;[Tan, Hua] Nanjing Univ Aeronaut & Astronaut, Acad Frontier Sci, Inst Bioinspired Struct & Surface Engn, Nanjing 210016, Peoples R China.;[Hu, Xin-Tian] Genom E China Normal Univ, Shanghai Inst Brain Funct, Shanghai 200062, Peoples R China.
通讯机构：
[Hu, Xin-Tian] C;Chinese Acad Sci, Kunming Inst Zool, Lab Sensory Motor Integrat Res, Kunming 650223, Yunnan, Peoples R China.
关键词：
Freely moving monkeys;Spatial learning and memory;Three-dimensional maze
摘要：
Many types of mazes have been used in cognitive brain research and data obtained from those experiments, especially those from rodents' studies, support the idea that the hippocampus is related to spatial learning and memory. But the results from non-human primates researches regarding the role of the hippocampus in spatial learning and memory are controversial and inconsistent with those obtained in rodents. This might be due to the differences of the methods used in non-human primates and rodents. Several kinds of maze models including two-dimensional computerized visual maze models and three-dimensional maze models have been developed for non-human primates, but they all have some defects. Therefore, development of a maze model for non-human primates that is comparable with those used in rodents is necessary to solve the controversy. This paper describes a large-scale, three-dimensional outdoor maze model for non-human primates which can be used to study spatial learning and memory. Monkeys learn to use the maze quickly compared with two-dimensional computerized visual mazes. It has many advantages which could make up the limits of the existing three-dimensional mazes in non-human primates, and can be comparable with radial arm mazes used in rodents. Based on the results, we believe that the new maze model will be valuable in many research areas, especially in studies involving spatial learning and memory in freely moving monkeys. (c) 2008 Elsevier B.V. All rights reserved.
作者：
Li, Guo Qing;Xia, Harry H. X.;Chen, Min Hu;Tsukamoto, Tetsuya;Tatematsu, Masae;...
期刊：
Helicobacter,2008年13(1):20-29 ISSN：1083-4389
通讯作者：
Wong, Benjamin C. Y.
作者机构：
[Xia, Harry H. X.; Wong, Benjamin C. Y.; Chan, Annie O. O.; Li, Guo Qing; Gu, Qing; Qiao, Liang; Yuen, M. F.] Univ Hong Kong, Queen Mary Hosp, Dept Med, Pokfulam, Hong Kong, Peoples R China.;[Hu, Pin Jin; Li, Guo Qing; Chen, Min Hu] Sun Yat Sen Univ, Affiliated Hosp 1, Dept Med, Guangzhou, Peoples R China.;[Li, Guo Qing] Nanhua Univ, Affiliated Hosp 2, Dept Med, Hengyang, Peoples R China.;[Tatematsu, Masae; Tsukamoto, Tetsuya] Aichi Canc Ctr, Res Inst, Div Oncol Pathol, Nagoya, Aichi 464, Japan.;[Cho, C. H.; So, Wallace H. L.] Univ Hong Kong, Dept Pharmacol, Pokfulam, Hong Kong, Peoples R China.
通讯机构：
[Wong, Benjamin C. Y.] U;Univ Hong Kong, Queen Mary Hosp, Dept Med, Pokfulam, Hong Kong, Peoples R China.
关键词：
H. pylori infection;aspirin;inflammation;heterotopic proliferative glands;apoptosis;cell proliferation
摘要：
Clinical observations have shown a link for the high comorbid rate between smoking and psychiatric disorders, including anxiety disorders. However, little is known about the neural mechanism underlying the progression from nicotine dependence to an anxiety disorder. A deficit in fear extinction in general is considered to contribute to anxiety disorders. The aim of the present study is to investigate the effects of chronic nicotine on fear extinction in rats. Rats were administrated s.c. nicotine twice per day for 14 days. Two weeks after the last injection rats received a cued or contextual fear conditioning session. Twenty-four hours and 48 h after conditioning, rats received an extinction training session and an extinction test session, respectively. Percent freezing was assessed during all phases of training. In the cued task, prior chronic nicotine did not affect the acquisition of fear response or the within-session fear extinction, but impaired the between-session fear extinction. In the contextual task, the same nicotine treatment schedule did not affect the acquisition of fear response or the within- and between-session fear extinction, but enhanced the retention of fear conditioning. This prior chronic nicotine-induced deficit in cued fear extinction and/or enhanced fear to context may be one of the critical components that contribute to the progression from nicotine dependence to an anxiety disorder.
期刊：
SURGICAL AND RADIOLOGIC ANATOMY,2008年30(1):77-81 ISSN：0930-1038
通讯作者：
Pei, Guo-xian
作者机构：
[Zhang, Yuan-zhi; Pei, Guo-xian; Li, Jian-wei] So Med Univ, Nanfang Hosp, Dept Orthopaed & Traumatol, Guangzhou, Peoples R China.;[Li, Yan-bing] Univ S China, Dept Anat, Hengyang, Peoples R China.;[Jiang, Yi-heng] So Med Univ, Inst Anat, Guangzhou, Peoples R China.;[Tang, Mao-lin] Wenzhou Med Coll, Dept Anat, Wenzhou, Peoples R China.
通讯机构：
[Pei, Guo-xian] S;So Med Univ, Nanfang Hosp, Dept Orthopaed & Traumatol, Guangzhou, Peoples R China.
