期刊论文

Liu Lu-Shan

中文

生物化学与生物物理进展

1000-3282

2008

35

5

540-547

10.3321/j.issn:1000-3282.2008.05.009

国家自然科学基金资助项目（30700325）； 湖南省教育厅资助项目（07C618）~~；

本校为第一且通讯机构

为研究低密度脂蛋白(LDL)、氧化修饰的低密度脂蛋白(oxLDL)对THP-1源性巨噬细胞中PCSK9、 LDLR表达的影响及两者之间的关系.分别用0mg/L、10mg/L、20mg/L、30mg/L的LDL和0mg/L、10mg/L、20mg/L、30mg/L的oxLDL处理THP-1巨噬细胞,油红O染色检测细胞荷脂情况,免疫荧光检测THP-1巨噬细胞上PCSK9蛋白表达及分布情况,RT-PCR、 Western blot检测THP-1巨噬细胞上PCSK9、 LDLR mRNA、蛋白质的表达.结果发现,不同浓度LDL处理THP-1巨噬细胞后,随着LDL浓度的增大,细胞内脂滴数目略有增多.免疫荧光染色发现,PCSK9在THP-1巨噬细胞上的表达随LDL浓度的增加而增多,胞浆内定位于某一特定细胞器中;RT-PCR、 Weste...

In order to study the effects of LDL and oxLDL on expression of PCSK9 and LDLR in THP-1 macrophages and find the relationship between them. THP-1 cells were induced to differentiate into macrophages by PMA treatment. Cells were then co-incubated with LDL or oxLDL with a concentration of 0 mg/L, 10 mg/L, 20 mg/L, 30 mg/L for 24h respectively. Cellular lipid was visualized by oil red 0 staining. The localization and semiquantitation of PCSK9 was confirmed by immunofluorescence assay, the expression of PCSK9 and LDLR was analyzed by RT-PCR and Western blotting. Oil red 0 staining showed that a nu...