作者机构：
[Ouyang, Xinping; Li, Jin-Feng; He, Pingping; Li, Yuanhui] Univ South China, Inst Cardiovasc Dis, Coll Med, Dept Physiol, Hengyang, Peoples R China.;[Wang, Bo; Liao, Huiying; Fu, Wan] Univ South China, First Affiliated Hosp, Hengyang, Peoples R China.;[Zhang, Tao] Univ South China, Second Affiliated Hosp, Hengyang, Peoples R China.;[He, Pingping] Univ South China, Sch Nursing, Hengyang, Peoples R China.
会议名称：
International Conference on Biological Engineering and Biomedical (BEAB)
会议时间：
JAN 10-12, 2014
会议地点：
Yichang, PEOPLES R CHINA
会议主办单位：
[Ouyang, Xinping;Li, Jin-Feng;Li, Yuanhui;He, Pingping] Univ South China, Inst Cardiovasc Dis, Coll Med, Dept Physiol, Hengyang, Peoples R China.^[Wang, Bo;Liao, Huiying;Fu, Wan] Univ South China, First Affiliated Hosp, Hengyang, Peoples R China.^[Zhang, Tao] Univ South China, Second Affiliated Hosp, Hengyang, Peoples R China.^[He, Pingping] Univ South China, Sch Nursing, Hengyang, Peoples R China.
摘要：
Objective: To test the effects of capsaicin on learning and memory ability in Alzheimer's disease (AD) mice. Results: Capsaicin (CAP) has no effect on spontaneous activity in APP/PS1 transgenic mice. CAP reduces the average escape latency and average swimming distance of Morris water maze in APP/PS1 transgenic mice. CAP adds academic record of Y maze in APP/PS1 transgenic mice. Conclusion: Capsaicin improves the function of learning and memory in AD mice.
作者机构：
[Wang Xiao-qing; Wang Li-li; Qu Peng; Wang Hong-yan; He Dan; Peng Kuang] Dalian Med Univ, Affiliated Hosp 2, Dept Cardiol, Dalian 116023, Liaoning, Peoples R China.;[Peng Kuang; Wang Gang] Univ South China, Affiliated Hosp 1, Vasculocardiol Dept, Henyang 421001, Hunan, Peoples R China.;[Wei Dang-heng; Liu Lu-shan] Univ South China, Inst Cardiovasc Dis, Henyang 421001, Hunan, Peoples R China.
会议名称：
25th Great Wall International Congress of Cardiology (GW-ICC) / Asia Pacific Heart Congress (APHC) / International Congress of Cardiovascular Prevention and Rehabilitation (ICCPR)
作者机构：
[Liu, Jun; Zhang, Tao] Univ South China, Affiliated Hosp 2, Dept Urol, Hengyang, Peoples R China.;[He, Pingping] Univ South China, Inst Cardiovasc Dis, Coll Med, Dept Physiol, Hengyang, Peoples R China.;[Chen, Xin; Li, E.] Hunan Tradit Chinese Med Coll, Zhuzhou, Peoples R China.;[Peng, Zhe; Wang, Bo; Liao, Huiying; Fu, Wan] Univ South China, First Affiliated Hosp, Hengyang, Peoples R China.;[He, Pingping] Univ S China, Sch Nursing, Hengyang, Peoples R China.
会议名称：
International Conference on Biological Engineering and Biomedical (BEAB)
会议时间：
JAN 10-12, 2014
会议地点：
Yichang, PEOPLES R CHINA
会议主办单位：
[He, Pingping] Univ South China, Inst Cardiovasc Dis, Coll Med, Dept Physiol, Hengyang, Peoples R China.^[Zhang, Tao;Liu, Jun] Univ South China, Affiliated Hosp 2, Dept Urol, Hengyang, Peoples R China.^[Chen, Xin;Li, E.] Hunan Tradit Chinese Med Coll, Zhuzhou, Peoples R China.^[Wang, Bo;Liao, Huiying;Fu, Wan;Peng, Zhe] Univ South China, First Affiliated Hosp, Hengyang, Peoples R China.^[He, Pingping] Univ S China, Sch Nursing, Hengyang, Peoples R China.
摘要：
Object: To observe the influence of silencing the expression of YB-1 gene by RNA interference cell on proliferation and migration of prostate cancer. Methods: The YB-1 siRNA was transfected into PC-3 cells at logarithmic growth by liposome, expression levels of YB-1 mRNA and protein were detected by reverse transcription-polymerase chain reaction (RT-PCR) and Wesretn-blot, proliferation and invasion of PC-3 cell was detected by MTT assay and Transwell experiments. Results: After transfection for 48 h, compared with PC-3 cells non-transfected with plasmid, YB-1 mRNA levels and YB-1 protein levels in PC-3 cells transfected with recombinant plasmid pGenesil-1-YB-1-1 and recombinant plasmid pGenesil-1-YB-1-2 were significantly decreased (P< 0.05). YB-1 mRNA inhibition rates in PC-3 cells transfected with recombinant plasmid pGenesil-1-YB-1-1 and recombinant plasmid pGenesil-1-YB-1-2 were 36.23% and 39.42%, while YB-1 protein inhibition rates were 41.56% and55.33%. MTT results showed that the proliferation of PC-3 cells transfected with recombinant plasmid pGenesil-1YB- 1-1 and recombinant plasmid pGenesil-1-YB-1-2 was significantly decreased when compared with PC-3 cells non-transfected with plasmid (P< 0.05). Transwell migration experiments showed that the number of cells go through the membrane in PC-3 cells non-transfected, PC-3 cells transfected with pGenesil-1-YB-NC, PC-3 cells transfected with recombinant plasmid pGenesil-1-YB-1-1 and PC-3 cells transfected with recombinant plasmid pGenesil-1-YB-1-2, were(123.71+/-13.25),(135.73+/-14.18),(67.73+/-8.82) and (51.73+/-6.75) respectively (P<0.05). The migration of PC-3 cells was reduced by downregulating the expression of YB-1, also the invasiveness of PC-3 cells vitro was inhibited. Conclusions: cell proliferation and migration of prostate cancer was inhibited by RNA interference silencing the expression of YB-1 gene.
作者机构：
[Huang He; Yuan Cong] Cent Hosp Xiangtan City, Dept Cardiol, Xiangtan 411100, Peoples R China.;[Liu Chang-hui; Wu Jie; Jie, W. U.] Univ South China, Affiliated Hosp, Dept Cardiol, Hengyang 421001, Peoples R China.;[Jiang Zhi-sheng] Univ South China, Inst Cardiovasc Dis, Hengyang 421001, Peoples R China.;[Jiang Zhi-sheng] Univ South China, Key Lab Arteriosclerol, Hengyang 421001, Peoples R China.;[Ouyang Ze-wei] Cent Hosp Shaoyang City, Dept Cardiol, Shaoyang 422000, Peoples R China.
会议名称：
25th Great Wall International Congress of Cardiology (GW-ICC) / Asia Pacific Heart Congress (APHC) / International Congress of Cardiovascular Prevention and Rehabilitation (ICCPR)
作者机构：
[Liu Chang-hui; Wu Jie; Yuan Cong; Zou Jin; Xu Ye-ping] Univ South China, Affiliated Hosp 1, Dept Cardiol, Hengyang, Peoples R China.;[Yuan Cong; Wen Ge-bo; Jiang Zhi-sheng] Univ South China, Inst Cardiovasc Dis, Hengyang, Peoples R China.;[Yuan Cong; Wen Ge-bo; Jiang Zhi-sheng] Univ South China, Key Lab Arteriosclerol, Hengyang, Peoples R China.
会议名称：
第二十四届长城国际心脏病学会议、亚太心脏大会暨国际心血管病预防与康复 会议(The 24th Great Wall International Congress of Cardiology & Asia Pacific Heart Congress & International Congress of Cardiovascular Prevention and Rehabilitation 2013)
会议时间：
2013-10-10
会议地点：
北京
会议主办单位：
中华医学会;长城国际心脏病学会议组委会
会议论文集名称：
第二十四届长城国际心脏病学会议、亚太心脏大会暨国际心血管病预防与康复 会议(The 24th Great Wall International Congress of Cardiology & Asia Pacific Heart Congress & International Congress of Cardiovascular Prevention and Rehabilitation 2
摘要：
The official journal of Sigma Theta Tau International, Honor Society of Nursing, Inc., Image is intended to provide a forum for the publication of superior nursing thought in the general areas of clinical scholarship and policy. Research reports, reviews of literature, and discursive pieces are regularly published.Coverage in the Journals&commat;Ovid database begins with the January 1995 issue.
作者：
Yang, S. H.;Brindley, P. J.;Zeng, Q. R.*;Zeng, T. B.;Li, Y. S.;...
作者机构：
[Li, Y. S.; Liu, Y.; Cai, L. T.; Liu, B. Y.; Zeng, T. B.; Lan, L. M.; Yang, S. H.; Zeng, Q. R.] Cent S Univ, Xiangya Sch Med, Ctr Cell & Mol Biol Expt, Changsha, Peoples R China.;[Wu, C. R.; Tan, Z. J.; Lu, F. G.; Yang, S. H.] Hunan Univ Chinese Med, Dept Pathogen Biol & Immunol, Changsha, Peoples R China.;[Brindley, P. J.] George Washington Univ, Med Ctr, Dept Microbiol Immunol & Trop Med, Washington, DC 20037 USA.;[Liu, Y.; Liu, B. Y.; Zeng, T. B.] Univ South China, Sch Med, Hengyang, Peoples R China.;[Li, Y. S.; McManus, D. P.] Queensland Inst Med Res, Mol Parasitol Lab, Brisbane, Qld, Australia.
会议名称：
12th International Congress of Parasitology (ICOPA)/6th Novel Approaches to the Control of Helminth Parasites of Liverstock Conference
会议时间：
AUG 15-20, 2010
会议地点：
Melbourne, AUSTRALIA
会议主办单位：
[Yang, S. H.;Zeng, Q. R.;Zeng, T. B.;Li, Y. S.;Liu, Y.;Liu, B. Y.;Cai, L. T.;Lan, L. M.] Cent S Univ, Xiangya Sch Med, Ctr Cell & Mol Biol Expt, Changsha, Peoples R China.^[Yang, S. H.;Wu, C. R.;Lu, F. G.;Tan, Z. J.] Hunan Univ Chinese Med, Dept Pathogen Biol & Immunol, Changsha, Peoples R China.^[Brindley, P. J.] George Washington Univ, Med Ctr, Dept Microbiol Immunol & Trop Med, Washington, DC 20037 USA.^[Zeng, T. B.;Liu, Y.;Liu, B. Y.] Univ South China, Sch Med, Hengyang, Peoples R China.^[Li, Y. S.;McManus, D. P.] Queensland Inst Med Res, Mol Parasitol Lab, Brisbane, Qld, Australia.^[Zhou, J.] Cent S Univ, Expt Ctr Xiangya Third Hosp, Changsha, Hunan, Peoples R China.
摘要：
Retroviral transduction of cultured schistosomes was explored in our study. The vesicular stomatitis virus glycoprotein (VSVG)-pseudotyped pantropic retroviral vector pBABE-puro was modified to incorporate the human telomerase reverse transcriptase gene (hTERT) as a reporter gene under the control of retroviral long terminal repeat (LTR). Pseudotyped virions were employed to transduce Schistosoma japonicum (Sj) to investigate the utility of retrovirus mediated Sj transgenesis and to investigate the activity as human telomerase as a reporter transgene in schistosomes. The presence of transgene hTERT was detected by PCR. Furthermore, analysis of RNAs from transduced parasites, immunohistochemistry of thin sections and immunoblot analysis revealed expression of hTERT transgene in the transduced worms. These findings indicated that Sj could be effectively transduced by VSVG pseudotyped retrovirus carrying the hTERT gene.
期刊：
Proceedings of the 2009 2nd International Conference on Biomedical Engineering and Informatics, BMEI 2009,2009年:1343-+ ISSN：1948-2914
通讯作者：
Wei Dang-heng
作者机构：
[Liu Lu-shang; Wang Zuo; Tang Chao-ke; Wei Dang-heng] Univ South China, Inst Cardiovasc Dis, Hengyang 421001, Peoples R China.;[Liu Lu-shang; Wang Zuo; Wang Gui-xue; Wei Dang-heng; Tang Chao-jun] Chongqing Univ, Bioengn Coll, Chongqing 400044, Peoples R China.;[Ruan Chang-geng; Wei Dang-heng] Suzhou Univ, Suzhou 215400, Peoples R China.
通讯机构：
[Wei Dang-heng] U;Univ South China, Inst Cardiovasc Dis, Hengyang 421001, Peoples R China.
关键词：
low density lipoprotein;concentration polarization;stenosis;endothelial cells;adhesion
摘要：
To test the hypothesis that concentration polarization of atherogenic lipids may occur in the arterial system and probe the effect of endothelial cells (ECs). A modifier with local stenosis was developed. The luminal surface LDL concentration at the distal of stenosis was analyzed with numerical stimulation and was measured by confocal microscopy. ECs were incubated with low density lipoprotein(LDL). Then, ECs were exposed to shear stress(15dyn/cm2) and F-actin was examined by fluorescence staining. Numerical stimulation showed that the luminal surface LDL concentration was higher than the bulk concentration. The relative luminal surface LDL concentration varied with the velocity and degree of stenosis. The experimental detection result was very good agreement with the numerical analysis. After treated with LDL, F-actin reorganization was observed. Under flow, the adhesion and retention of ECs were negatively relative to LDL concentration. Therefore provided concentration polarization of LDL occurs at the distal end of stenosis, and in turn effect the adhesion of ECs under flow.
