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Effect of the short hairpin (shRNA)-SR-PSOX on smooth muscle cells migration

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成果类型:
会议论文
作者:
Xu, Yang-Yan*;Wu, Duan-Sheng;Yang, Hui-Ling;Chen, Wei-Qiong;Li ZiShan;Peng, Hui-Bing;Quan, Zhi-Hua
通讯作者:
Xu, Yang-Yan
作者机构:
[Li ZiShan; Yang, Hui-Ling; Xu, Yang-Yan; Quan, Zhi-Hua; Peng, Hui-Bing; Wu, Duan-Sheng] Univ South China, Affiliated Hosp 1, Inst Clin Med, Hengyang 421001, Hunan, Peoples R China.
[Yang, Hui-Ling; Xu, Yang-Yan; Wu, Duan-Sheng; Chen, Wei-Qiong] Univ South China, Inst Pharmacol & Pharmacy, Hengyang 421001, Hunan, Peoples R China.
通讯机构:
[Xu, Yang-Yan] Univ South China, Affiliated Hosp 1, Inst Clin Med, Hengyang 421001, Hunan, Peoples R China.
语种:
英文
关键词:
Scavenger receptor binds phosphatidylserine and oxidized lipoprotein (SR-PSOX);short hairpin RNA(shRNA);lentiviral expression system;atherosclerosis
期刊:
Proceedings of the 2009 2nd International Conference on Biomedical Engineering and Informatics, BMEI 2009
ISSN:
1948-2914
年:
2009
页码:
801-+
会议名称:
2009 2nd International Conference on Biomedical Engineering and Informatics, BMEI 2009
会议论文集名称:
International Conference on Biomedical Engineering and Informatics
会议时间:
October 17, 2009 - October 19, 2009
会议地点:
Tianjin, China
会议主办单位:
(1) Institute of Clinical Medicine, University of South China, Hengyang, 421001, Hunan Province, China; (2) Institute of Pharmacology and Pharmacy, University of South China, Western Changsheng Road 28, Hengyang 421001, Hunan Province, China
会议赞助商:
IEEE Engineering in Medicine and Biology Society
主编:
Shi, R Fu, WJ Wang, YQ Wang, HB
出版地:
345 E 47TH ST, NEW YORK, NY 10017 USA
出版者:
IEEE Computer Society, 445 Hoes Lane - P.O.Box 1331, Piscataway, NJ 08855-1331, United States
ISBN:
978-1-4244-4133-4
机构署名:
本校为第一且通讯机构
院系归属:
医学院
药学与生物科学学院
摘要:
To investigate the effect of shRNA-SR-PSOX (Scavenger receptor binds phosphatidylserine and oxidized lipoprotein) on smooth muscle cells(SMC) migration, we designed and synthesised the shRNA oligonucleotide targeted human SR-PSOX gene, and constructed the shRNA lentiviral expression system in vitro to infect HepG2 cell line, and the silencing efficiency was assayed by real-time reverse transcription-polymerase chain reaction, immunofluorescence staining, Western blotting and Transwell chambers. The reverse transcription-polymerase chain reaction (RT-PCR) results showed that the potent inhibition of SR-PSOX expression could reach 80%, and it was specific to the SR-PSOX -derived shRNA, not the Caveolin-1-derived shRNA (negative shRNA control). Indirect immunofluorescence and Western blot results showed the SR-PSOX expression decreased obviously in SR-PSOX-shRNA transfected group compared with control group. In addition, transwell chambers results indicated that these SR-PSOX over-expressing cells showed ˜3 fold increased migration of SMCs compared with normal SMC cells or siRNA cells. Red oil staining found that SR-PSOX over expressed SMCs had more lipid droplets, while lipid droplets in siRNA-SR-PSOX vector groups decreased significantly. All these data indicated that blockage and downregulation SR-PSOX expression is a potential therapeutic target to prevent inflammatory AS damage. ©2009 IEEE.

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