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Effect of the short hairpin (shRNA)-SR-PSOX on smooth muscle cells migration

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成果类型:
期刊论文
作者:
Xu, Yang-Yan*;Wu, Duan-Sheng;Yang, Hui-Ling;Chen, Wei-Qiong;Li ZiShan;...
通讯作者:
Xu, Yang-Yan
作者机构:
[Li ZiShan; Yang, Hui-Ling; Xu, Yang-Yan; Quan, Zhi-Hua; Peng, Hui-Bing; Wu, Duan-Sheng] Univ South China, Affiliated Hosp 1, Inst Clin Med, Hengyang 421001, Hunan, Peoples R China.
[Yang, Hui-Ling; Xu, Yang-Yan; Wu, Duan-Sheng; Chen, Wei-Qiong] Univ South China, Inst Pharmacol & Pharmacy, Hengyang 421001, Hunan, Peoples R China.
通讯机构:
[Xu, Yang-Yan] U
Univ South China, Affiliated Hosp 1, Inst Clin Med, Hengyang 421001, Hunan, Peoples R China.
语种:
英文
关键词:
Scavenger receptor binds phosphatidylserine and oxidized lipoprotein (SR-PSOX);short hairpin RNA(shRNA);lentiviral expression system;atherosclerosis
期刊:
Proceedings of the 2009 2nd International Conference on Biomedical Engineering and Informatics, BMEI 2009
ISSN:
1948-2914
年:
2009
页码:
801-+
机构署名:
本校为第一且通讯机构
院系归属:
医学院
药学与生物科学学院
摘要:
To investigate the effect of shRNA-SR-PSOX (Scavenger receptor binds phosphatidylserine and oxidized lipoprotein) on smooth muscle cells(SMC) migration, we designed and synthesised the shRNA oligonucleotide targeted human SR-PSOX gene, and constructed the shRNA lentiviral expression system in vitro to infect HepG2 cell line, and the silencing efficiency was assayed by real-time reverse transcription-polymerase chain reaction, immunofluorescence staining, Western blotting and Transwell chambers. The reverse transcription-polymerase chain reaction (RT-PCR) results showed that the potent inhibiti...

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