National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [30970721]; Ministry of Education of ChinaMinistry of Education, China [104158]; National "111 plan" [B06023]
机构署名:
本校为其他机构
院系归属:
医学院
摘要:
In this study, we investigated the effect of low density lipoprotein (LDL) on endothelial cell adhesion in shear stress. The endothelial cells were incubated with four different concentrations of low density lipoprotein for 24h. Thereafter, the content of cholesterol ester in endothelial cells was assayed with High Performance Liquid Chromatography (HPLC). The lipid within endothelial cells was detected with oil red O. F-actin was examined with fluorescence staining of rhodamine-phalloidin. A micropipette aspiration technique was employed to in...