摘要：
Theoretical investigations are conducted to examine the impact of toroidal rotation and ion collisional viscosity on low-frequency zonal flow (LFZF) in a tokamak plasma with the fluid model. The results indicate that while the frequency of LFZF remains unchanged during an isothermal process with
$$\gamma { = }1$$
, its damping rate is influenced by both toroidal rotation and ion collisional viscosity. Specifically, as the ion collisional viscosity increases, so does the damping rate of LFZF; conversely, as the toroidal rotation As its Mach number rises, its damping rate decreases. For a non-isothermal process with
$$\gamma \, > 1$$
(such as
$$\gamma = 5/3$$
), the damping rate and frequency of LFZF are influenced by both toroidal rotation and ion collisional viscosity. It has been demonstrated that when the toroidal rotation Mach number surpasses a certain threshold, the frequency transitions from zero to a finite value, while the damping rate decreases as the toroidal rotation Mach number increases for LFZF. Furthermore, it has been observed that the damping rate of LFZF increases while the frequency decreases with an increase in ion collisional viscosity. Moreover, when the ion collisional viscosity surpasses a certain threshold, the frequency of LFZF drops to zero.
摘要：
Land-based uranium resources are becoming scarce because of the widespread development and use of nuclear energy. Therefore, to make up for the shortage of uranium resources, a new chitosan/carboxymethyl-β-cyclodextrin/quaternary ammonium salt-functionalized amidoxime carbon adsorbent (CSAOCF) was designed and synthesized for extracting uranium from seawater. Experimental studies show that the adsorption of uranium by CSAOCF is a spontaneous endothermic reaction and chemical adsorption. The theoretical maximum adsorption capacity of uranium can reach 726mg/g at 308K and pH=6. Moreover, the adsorption efficiency and selectivity of CSAOCF for uranium were significantly improved after the introduction of the carboxymethyl group, and the selection and partition coefficient of CSAOCF for uranium and vanadium increased from 16-fold to 30-fold under the same conditions. This indicates that there is a synergistic effect between carboxyl and amidoxime groups, which can promote the adsorption of uranium by CSAOCF. Furthermore, CSAOCF exhibits good oil resistance and can be reused more than five times. Therefore, CSAOCF containing carboxymethyl and amidoxime functional groups can considerably improve the selective adsorption of uranium and has great potential in the extraction of uranium from seawater.
关键词：
Nonradical oxidation;Ni-N4;Peroxymonosulfate;Charge transfer process;High-valent Ni species
摘要：
In this work, we revealed a dual -pathway nonradical oxidation (NRO) system catalyzed by Ni single atom catalyst (NiN4) accommodated in the carbon nitride substrate. The monatomic Ni coordinated with four N atoms (Ni-N4) is determined as the dynamic catalytic center for peroxymonosulfate (PMS) activation, showing an exceptional specific rate constant of 3.34 min -1 g-2 L-2 (29.7 times that of CN/PMS system) in oxidizing bisphenol A and other refractory organics. In situ Raman, X-ray absorption, and electrochemical analyses as well as theoretical simulations demonstrate that the Ni-N4 sites exhibit strong interaction with PMS benefited from the modulated electronic structures, resulting in nonradical surface -bonding active complexes (NiN4-PMS*) and deuterogenic high-valent Ni(IV)-Oxo species. The dual -pathway NRO process will spontaneously coordinate both electron transfer process via CN matrix and direct electron transfer by Ni(IV)-Oxo for selective organic oxidation with excellent anti -interference ability and adaptability.
摘要：
To investigate the strengthening effects and mechanisms of bioaugmentation on the microbial remediation of uranium-contaminated groundwater via bioreduction coupled to biomineralization, two exogenous microbial consortia with reducing and phosphate-solubilizing functions were screened and added to uranium-contaminated groundwater as the experimental groups (group B, reducing consortium added; group C, phosphate-solubilizing consortium added). β-glycerophosphate (GP) was selected to stimulate the microbial community as the sole electron donor and phosphorus source. The results showed that bioaugmentation accelerated the consumption of GP and the proliferation of key functional microbes in groups B and C. In group B, Dysgonomonas, Clostridium_sensu_stricto_11 and Clostridium_sensu_stricto_13 were the main reducing bacteria, and Paenibacillus was the main phosphate-solubilizing bacteria. In group C, the microorganisms that solubilized phosphate were mainly unclassified_f_Enterobacteriaceae. Additionally, bioaugmentation promoted the formation of unattached precipitates and alleviated the inhibitory effect of cell surface precipitation on microbial metabolism. As a result, the formation rate of U-phosphate precipitates and the removal rates of aqueous U(VI) in both groups B and C were elevated significantly after bioaugmentation. The U(VI) removal rate was poor in the control group (group A, with only an indigenous consortium). Propionispora, Sporomusa and Clostridium_sensu_stricto_11 may have played an important role in the removal of uranium in group A. Furthermore, the addition of a reducing consortium promoted the reduction of U(VI) to U(IV), and immobilized uranium existed in the form of U(IV)-phosphate and U(VI)-phosphate precipitates in group B. In contrast, U was present mainly as U(VI)-phosphate precipitates in groups A and C. Overall, bioaugmentation with an exogenous consortium resulted in the rapid removal of uranium from groundwater and the formation of U-phosphate minerals and served as an effective strategy for improving the treatment of uranium-contaminated groundwater in situ.
期刊：
Journal of Hazardous Materials,2024年461:132664 ISSN：0304-3894
通讯作者：
Yin, XB;Wang, XP
作者机构：
[Fujita, Toyohisa; Wang, Xinpeng; Lin, Zheyang; Chen, Yuliang] Guangxi Univ, Sch Resources Environm & Mat, State Key Lab Featured Met Mat & Life Cycle Safety, 100 Daxue East Rd, Nanning 530004, Peoples R China.;[Yin, Xiangbiao; Ning, Shunyan; Zheng, Ningchao] Univ South China, Sch Nucl Sci & Technol, 28 Changsheng West Rd, Hengyang 421001, Peoples R China.;[Chen, Yanliang] East China Inst Technol, Minist Educ, Engn Res Ctr Nucl Technol Applicat, Nanchang 330013, Peoples R China.;[Wang, Xinpeng; Wang, XP] Guangxi Univ, Sch Resources Environm & Mat, 100 Daxue East Rd, Nanning 530004, Peoples R China.
通讯机构：
[Wang, XP ] G;[Yin, XB ] U;Univ South China, Sch Nucl Sci & Technol, 28 Changsheng West Rd, Hengyang 421001, Peoples R China.;Guangxi Univ, Sch Resources Environm & Mat, 100 Daxue East Rd, Nanning 530004, Peoples R China.
关键词：
Electro adsorption;NASICON;Seawater;Uranium
摘要：
As an eco-friendly technique with the superior adsorption performance, electroadsorption has shown great potential for application in uranium (U(VI)) recovery in recent years. However, the electrodes used in the electrosorption generally suffer the adsorbent to be loaded on the conductors, which greatly limited the adsorption performance of the electrodes for uranyl ions. In present study, a flexible self-supporting Na(3)MnTi(PO(4))(3)@C fibers (NMTP@C fibers) electrode material was rationally designed and prepared by electrostatic spinning method and annealing technique, and its ability to capture U(VI) efficiently was preliminarily demonstrated by batch adsorption and electro sorption. The plentiful phosphate groups provide sufficient active sites for adsorption, while the axially continuous electron conduction and radially short-range ion transport give NMTP@C fibers fast charge/ion transport capability. The NMTP@C fiber can remove 99% of 5ppmU(VI) in seawater by electro absorption within 1h. After several cycles of adsorption under seawater conditions, the adsorbent can still maintain a stable adsorption capacity. The adsorption mechanism of NMTP@C nanofibers for U(VI) was investigated by XPS, FT-IR, Raman, SEM-EDS, and XRD, which was electrostatic interactions and surface complexation. These results suggest that NMTP@C fibers are promising high-capacity adsorbents for efficient and selective capture of U(VI) from seawater.
摘要：
Gram-negative Bartonella species are facultative intracellular bacteria that can survive in the harsh intracellular milieu of host cells. They have evolved strategies to evade detection and degradation by the host immune system, which ensures their proliferation in the host. Following infection, Bartonella alters the initial immunogenic surface-exposed proteins to evade immune recognition via antigen or phase variation. The diverse lipopolysaccharide structures of certain Bartonella species allow them to escape recognition by the host pattern recognition receptors. Additionally, the survival of mature erythrocytes and their resistance to lysosomal fusion further complicate the immune clearance of this species. Certain Bartonella species also evade immune attacks by producing biofilms and anti-inflammatory cytokines and decreasing endothelial cell apoptosis. Overall, these factors create a challenging landscape for the host immune system to rapidly and effectively eradicate the Bartonella species, thereby facilitating the persistence of Bartonella infections and creating a substantial obstacle for therapeutic interventions. This review focuses on the effects of three human-specific Bartonella species, particularly their mechanisms of host invasion and immune escape, to gain new perspectives in the development of effective diagnostic tools, prophylactic measures, and treatment options for Bartonella infections.
期刊：
JOURNAL OF SUPERCRITICAL FLUIDS,2024年205:106145 ISSN：0896-8446
通讯作者：
Ge, FH;Wang, WG
作者机构：
[Fang, Ting; Ge, Fahuan; Ge, FH; Zhou, Dan; Duan, Minghui; Zhou, Xue] Sun Yat Sen Univ, Sch Pharmaceut Sci, Guangzhou 510006, Peoples R China.;[Wang, Weiguo; Duan, Minghui] Univ South China, Hengyang 421000, Hunan, Peoples R China.;[Fang, Ting; Ge, Fahuan; Ge, FH; Zhou, Dan] Guangdong Engn Res Ctr Supercrit Fluid Extract Tra, Guangzhou 510006, Peoples R China.;[Shi, Qinglong] Natl Engn Res Ctr Pharmaceut Proc Technol Tradit C, Guangzhou, Peoples R China.
通讯机构：
[Ge, FH ] S;[Wang, WG ] U;Sun Yat Sen Univ, Sch Pharmaceut Sci, Guangzhou 510006, Peoples R China.;Univ South China, Hengyang 421000, Hunan, Peoples R China.;Guangdong Engn Res Ctr Supercrit Fluid Extract Tra, Guangzhou 510006, Peoples R China.
关键词：
High-pressure supercritical CO 2 extraction;Astaxanthin;Haematococcus pluvialis;Kinetics
摘要：
Astaxanthin derived from Hematococcus pluvialis (H. pluvialis) exhibits potent capacity in scavenging free radicals and quenching active oxygen. However, the conventional extraction of Astaxanthin from H. pluvialis involves the use of a substantial amount of organic reagents, leading to environmental pollution and low extraction efficiency. In this work, a high-pressure supercritical CO2 extraction (H-SFE) was applied to extract astaxanthin from H. pluvialis. Influential parameters of H-SFE were optimized by single factor, orthogonal test and kinetics experiments. Extraction pressure is a critical component influencing the extraction efficiency of SFE. Increased pressure results in increased supercritical CO2 density and thus increased solubility of substances and enhanced component extraction. The extraction yield of astaxanthin at 65 MPa was 1.25 times and 2.32 times higher than that at 45 MPa and 25 MPa, respectively. The H-SFE extraction yield of astaxanthin was 20%−70% higher than that of ultrasonic-assisted extraction (UAE) and organic solvent extraction (OSE).
摘要：
A huge solid cystic mass located in the mediastinum was found in a 15‐year‐old youngster suffering shortness of breath. The synovial sarcoma was diagnosed by tissue of emergency surgery. One month later, the mediastinum lesion of patient relapsed. The next‐generation sequencing was performed and revealed BRCA2 c.968dupT mutation. Eventually, the pazopanib plus nivolumab was administered with progression‐free survival of 14 months. Abstract Pericardial synovial sarcomas (PSS) have a low incidence rate and are highly invasive with a dismal prognosis. Standard treatment includes surgery, radiotherapy and chemotherapy but with limited response. Here, we report the case of a 15‐year‐old nonsmoking youngster diagnosed with PSS who developed disease relapsed from surgery after 1 month. Next‐generation sequencing (NGS) using baseline tissue was performed, and BRCA2 c.968dupT was detected. Then pazopanib (a multitargeted inhibitor) plus nivolumab (an immune checkpoint inhibitor) was administered, with a partial response and progression‐free survival of 14 months. BRCA2 c.968dupT has not previously been reported in PSS and its response to targeted combination immunotherapy are not well characterized. Here, we report the efficacy of pazopanib combined with nivolumab in a PSS patient harboring BRCA2 c.968dupT and also provide the clinical evidence of the utility of NGS in exploring actionable mutations for solid tumor. Combination therapy based on immunotherapy may be a potential treatment choice for PSS harboring BRCA2 mutation.
摘要：
Antibiotics and antibiotic resistance genetic pollution have become a global environmental and health concern recently, with frequent detection in various environmental media. Therefore, finding ways to control antibiotics and antibiotic resistance genes (ARGs) is urgently needed. Nano zero-valent iron (nZVI) has shown a positive effect on antibiotics degradation and restraining ARGs, making it a promising solution for controlling antibiotics and ARGs. However, given the current increasingly fragmented research focus and results, a comprehensive review is still lacking. In this work, we first introduce the origin and transmission of antibiotics and ARGs in various environmental media, and then discuss the affecting factors during the degradation of antibiotics and the control of ARGs by nZVI and modified nZVI, including pH, nZVI dose, and oxidant concentration, etc. Then, the mechanisms of antibiotic and ARGs removal promoted by nZVI are also summarized. In general, the mechanism of antibiotic degradation by nZVI mainly includes adsorption and reduction, while promoting the biodegradation of antibiotics by affecting the microbial community. nZVI can also be combined with persulfates to degrade antibiotics through advanced oxidation processes. For the control of ARGs, nZVI not only changes the microbial community structure, but also affects the proliferation of ARGs through affecting the fate of mobile genetic elements (MGEs). Finally, some new ideas on the application of nZVI in the treatment of antibiotic resistance are proposed. This paper provides a reference for research and application in this field.
摘要：
Tumor cell metastasis is the key cause of death in patients with nasopharyngeal carcinoma (NPC). MiR-2110 was cloned and identified in Epstein-Barr virus (EBV)-positive NPC, but its role is unclear in NPC. In this study, we investigated the effect of miR-2110 on NPC metastasis and its related molecular basis. In addition, we also explored whether miR-2110 can be regulated by cinobufotalin (CB) and participate in the inhibition of CB on NPC metastasis. Bioinformatics, RT-PCR, and in situ hybridization were used to observe the expression of miR-2110 in NPC tissues and cells. Scratch, Boyden, and tail vein metastasis model of nude mouse were used to detect the effect of miR-2110 on NPC metastasis. Western blot, Co-IP, luciferase activity, colocalization of micro confocal and ubiquitination assays were used to identify the molecular mechanism of miR-2110 affecting NPC metastasis. Finally, miR-2110 induced by CB participates in CB-stimulated inhibition of NPC metastasis was explored. The data showed that increased miR-2110 significantly suppresses NPC cell migration, invasion, and metastasis. Suppressing miR-2110 markedly restored NPC cell migration and invasion. Mechanistically, miR-2110 directly targeted FGFR1 and reduced its protein expression. Decreased FGFR1 attenuated its recruitment of NEDD4, which downregulated NEDD4-induced phosphatase and tensin homolog (PTEN) ubiquitination and degradation and further increased PTEN protein stability, thereby inactivating PI3K/AKT-stimulated epithelial-mesenchymal transition signaling and ultimately suppressing NPC metastasis. Interestingly, CB, a potential new inhibitory drug for NPC metastasis, significantly induced miR-2110 expression by suppressing PI3K/AKT/c-Jun-mediated transcription inhibition. Suppression of miR-2110 significantly restored cell migration and invasion in CB-treated NPC cells. Finally, a clinical sample assay indicated that reduced miR-2110 was negatively correlated with NPC lymph node metastasis and positively related to NPC patient survival prognosis. In summary, miR-2110 is a metastatic suppressor involving in CB-induced suppression of NPC metastasis.
摘要：
Bladder carcinoma represents a prevalent malignancy, wherein the influence of sex extends across its incidence, biological attributes, and clinical outcomes. This scholarly exposition meticulously examines pertinent investigations, elucidating the nuanced impact of sex on bladder cancer, and posits cogent avenues for future research and intervention modalities. In the initial discourse, an exhaustive scrutiny is undertaken of the etiological underpinnings of bladder cancer, encompassing variables such as tobacco consumption, occupational exposures, and genetic aberrations. Subsequently, a comprehensive dissection unfolds, delving into the intricate biological disparities inherent in sex vis-à-vis the initiation and progression of bladder cancer. This analytical framework embraces multifaceted considerations, spanning sex hormones, sex chromosomal dynamics, metabolic enzymatic cascades, and the intricate interplay with the microbiome. Lastly, a synthesized exposition encapsulates the ramifications of gender differentials on the diagnostic and prognostic landscapes of bladder cancer, underscoring the imperative for intensified investigative endeavors directed towards elucidating gender-specific variances and the formulation of tailored therapeutic strategies.
摘要：
BACKGROUND: Potassium bismuth citrate is a gastric mucosal protector and a key drug for treating peptic ulcers. OBJECTIVE: To evaluate the pharmacokinetic characteristics and safety of 120-mg bismuth potassium citrate formulations administered orally under fasting conditions in healthy Chinese subjects. METHOD: A single-center open two-cycle trial was conducted on 12 healthy subjects who received a single oral dose of 120 mg of bismuth potassium citrate. The plasma concentration of bismuth was determined using a validated inductively coupled plasma mass spectrometry (ICP‒MS) method. The pharmacokinetic parameters, including maximum serum concentration (C(max)) and area under the curve concentration-time curve (AUC(0-t) and AUC(0-∞)), and safety were evaluated via noncompartment analysis. RESULTS: The ratios of the least square geometric mean ratio between the test (T) and reference (R) formulations for C(max), AUC(0-t), and AUC(0-∞) were 44.8%, 55.5%, and 64.4%, respectively; the bilateral 95% confidence intervals (Cis) for these parameters were 20.2-99.6%, 24.1-127.5%, and 23.7-175.0%, respectively, and the non-inferior limits for these parameters were 169.4%, 198.8%, and 200.5%, respectively. The upper limits of the one-sided 97.5% confidence interval for the least squares geometric mean ratio (T/R) were lower than the non-inferior limits. No serious adverse reactions or adverse reactions leading to detachment were observed among the subjects. CONCLUSION: The concentration of bismuth in the blood of healthy subjects in the T formulation was not greater than that in the R formulation. Similarly, the safety of oral administration of 120 mg of bismuth potassium citrate formulations to healthy subjects was good. The trial registration number (TRN) was [2018] 013, 6 December 2018.
作者机构：
[Wei, Hua; Cheng, Yao; Zhang, Haitao; Yu, Cui-Yun; Wei, H] Univ South China, Hunan Prov Cooperat Innovat Ctr Mol Target New Dru, 28 W Changsheng Rd, Hengyang 421001, Hunan, Peoples R China.;Univ South China, Sch Pharmaceut Sci, Hengyang Med Sch, 28 W Changsheng Rd, Hengyang 421001, Hunan, Peoples R China.
通讯机构：
[Zhang, HT; Yu, CY ; Wei, H] U;Univ South China, Hunan Prov Cooperat Innovat Ctr Mol Target New Dru, 28 W Changsheng Rd, Hengyang 421001, Hunan, Peoples R China.
摘要：
Tumor therapy continues to be a prominent field within biomedical research. The development of various drug carriers has been propelled by concerns surrounding the side effects and targeting efficacy of various chemotherapeutic drugs and other therapeutic agents. These carriers strive to enhance drug concentration at tumor sites, minimize systemic side effects, and improve therapeutic outcomes. Among the reported delivery systems, injectable hydrogels have emerged as an emerging candidate for the in vivo delivery of chemotherapeutic drugs due to their minimal invasive drug delivery properties. This review systematically summarizes the composition and preparation methodologies of injectable hydrogels and further highlights the delivery mechanisms of diverse drugs using these hydrogels for tumor therapy, along with an in-depth discussion on the optimized therapeutic efficiency of drugs encapsulated within the hydrogels. The work concludes by providing a dynamic forward-looking perspective on the potential challenges and possible solutions of the in situ injectable hydrogels for non-surgical and real-time diagnostic applications. This review summarizes composition and preparation methodologies of injectable hydrogels and highlights the delivery mechanisms of drugs for tumor therapy, along with discussion on the optimal therapeutic efficiency of drugs and provides perspective on challenges and solutions in applications.
摘要：
BACKGROUND: High myopia (HM) refers to an eye refractive error exceeding -5.00 D, significantly elevating blindness risk. The underlying mechanism of HM remains elusive. Given the extensive genetic heterogeneity and vast genetic base opacity, it is imperative to identify more causative genes and explore their pathogenic roles in HM. METHODS: We employed exome sequencing to pinpoint the causal gene in an HM family. Sanger sequencing was used to confirm and analyse the gene mutations in this family and 200 sporadic HM cases. Single-cell RNA sequencing was conducted to evaluate the gene's expression patterns in developing human and mouse retinas. The CRISPR/Cas9 system facilitated the gene knockout cells, aiding in the exploration of the gene's function and its mutations. Immunofluorescent staining and immunoblot techniques were applied to monitor the functional shifts of the gene mutations at the cellular level. RESULTS: A suspected nonsense mutation (c.C172T, p.Q58X) in CCDC66 was found to be co-segregated with the HM phenotype in the family. Additionally, six other rare variants were identified among the 200 sporadic patients. CCDC66 was consistently expressed in the embryonic retinas of both humans and mice. Notably, in CCDC66-deficient HEK293 cells, there was a decline in cell proliferation, microtube polymerisation rate and ace-tubulin level. Furthermore, the mutated CCDC66 failed to synchronise with the tubulin system during Hela cell mitosis, unlike its wild type counterpart. CONCLUSIONS: Our research indicates that the CCDC66 variant c.C172T is associated with HM. A deficiency in CCDC66 might disrupt cell proliferation by influencing the mitotic process during retinal growth, leading to HM.
期刊：
Reliability Engineering & System Safety,2024年243:109827 ISSN：0951-8320
通讯作者：
Ohl, JP
作者机构：
[Jiadong, Qiu] Univ South China, Sch Resources Environm & Safety Engn, Hengyang 421001, Peoples R China.;[Ohl, Joy P.] NanoStructuraCivis Lab Adv Smart Mat Civil Engn, Tokyo, Japan.;[Tran, Trung-Tin] Swinburne Vietnam FPT Univ, Dept Informat Technol, Da Nang, Vietnam.
通讯机构：
[Ohl, JP ] N;NanoStructuraCivis Lab Adv Smart Mat Civil Engn, Tokyo, Japan.
关键词：
Clay compressibility;Artificial neural network;Metaheuristic algorithms;Prediction accuracy;Practical engineering;Reliability systems
摘要：
This study aims at predicting clay compressibility (clay compressibility) for foundation design with unprecedented reliability and safety. To achieve this goal, we set forth several scientific objectives to enhance the ac-curacy of clay compressibility predictions and ensure the robustness of our models. Firstly, we explored an updated dataset comprising diverse clay types, focusing on the intricate relationship between clay compressibility and essential soil properties. By incorporating particle size distribution, the ratio between liquid limit (wLL) and plasticity index (PI), and the ratio between void ratio (eLL) and wLL, our objective was to gain a comprehensive understanding of clay behavior, facilitating more accurate predictions. Secondly, we employed Artificial Neural Networks (ANNs) as our primary modeling tool. ANNs, with their capacity to discern complex patterns within data, formed the foundation of our predictive model. Our scientific objective was to harness the power of ANNs to capture subtle relationships among input variables, thereby ensuring a robust foundation for clay compressibility predictions. To further refine our predictions, our third objective was to optimize the ANN model. We utilized five distinct metaheuristic algorithms-Bird Swarm Algorithm (BSA), Dragonfly Optimization (DO), Elephant Herding Optimization (EHO), Jaya Algorithm (JA), and Whale Optimization Algorithm (WOA). Our aim was to fine-tune the ANN model's parameters using these algorithms, capitalizing on their strengths to achieve superior predictive performance. Additionally, our study aimed to pioneer the development and eval-uation of hybrid models for forecasting clay compressibility. By integrating the ANN model with the meta-heuristic algorithms (BSA-ANN, DO-ANN, EHO-ANN, JA-ANN, and WOA-ANN), our objective was to create hybrid frameworks that synergistically combined the strengths of both approaches. We rigorously evaluated these hybrid models, ensuring their efficacy and reliability in practical engineering applications. Crucially, we validated our models through practical engineering scenarios. For this purpose, we prepared 30 new soil samples mirroring real-world conditions. Our objective was to apply the developed models to these samples, affirming their effectiveness and reliability in actual foundation design applications. In pursuit of identifying the most accurate and reliable predictive model, our final objective focused on the BSA-ANN model. Remarkably, this model demonstrated an exceptional accuracy of 99.8%. Our objective was to establish the BSA-ANN model as the preferred choice for practical engineering applications, ensuring high reliability and safety in foundation design.
摘要：
Commercially pure titanium (CP-Ti) took advantages of low stiffness, high corrosion resistance and biocompatibility, implying an ideal structural material in aerospace, military and medical industries. However, its insufficient mechanical properties have always been the long-standing obstacle for the industrial applications. In this work, we have achieved a superior strong and ductile CP-Ti via active atmosphere -assisted selective laser melting (SLM) and subsequent rolling treatment. By incorporating the active N2 atmosphere during SLM, mechanical properties of CP-Ti were substantially improved, microhardness of the lyaers with 10 at.% and 100 at.% was 63 % and 174.3 % higher than that of CP-Ti with 182.3 HV0.2, the compressive strength of 10 sample was 27.1 % higher than that of 10 L sample when the strain was 52.2 %, and ultimate tensile strength of 10 sample reached 928 MPa with an elongation of 25.6 %, and ultimate tensile strength and elongation of 10 L sample were enhanced simultaneously by 58.3 % and 13.6 % relative to CP-Ti sample. These were attributed to the interstitial strengthening induced by N atoms. After rolling treatment, the mechanical properties of N -doping layers could enhance furtherly. Macroscale cracking along the rolling direction never occur in the 10 L sample during the repeatedly rolling treatment, which could depend to high tolerability of sandwich layers for high -density dislocations. Meanwhile, rolling treatment played an important role for refining grain to enhance the strength further.
关键词：
cancer therapy;cellular metabolism;ferroptosis inducer;ferroptosis
摘要：
Cellular metabolism is the fundamental process by which cells maintain growth and self-renewal. It produces energy, furnishes raw materials, and intermediates for biomolecule synthesis, and modulates enzyme activity to sustain normal cellular functions. Cellular metabolism is the foundation of cellular life processes and plays a regulatory role in various biological functions, including programmed cell death. Ferroptosis is a recently discovered form of iron-dependent programmed cell death. The inhibition of ferroptosis plays a crucial role in tumorigenesis and tumor progression. However, the role of cellular metabolism, particularly glucose and amino acid metabolism, in cancer ferroptosis is not well understood. Here, we reviewed glucose, lipid, amino acid, iron and selenium metabolism involvement in cancer cell ferroptosis to elucidate the impact of different metabolic pathways on this process. Additionally, we provided a detailed overview of agents used to induce cancer ferroptosis. We explained that the metabolism of tumor cells plays a crucial role in maintaining intracellular redox homeostasis and that disrupting the normal metabolic processes in these cells renders them more susceptible to iron-induced cell death, resulting in enhanced tumor cell killing. The combination of ferroptosis inducers and cellular metabolism inhibitors may be a novel approach to future cancer therapy and an important strategy to advance the development of treatments.
通讯机构：
[Zeng, WM ] C;Cent South Univ, Sch Minerals Proc & Bioengn, Changsha 410083, Peoples R China.
关键词：
vermiculite additive;co-composting;microbial community;co-network analysis;community function
摘要：
Vermiculite is a clay mineral with unique physical properties that plays a significant role in plant cultivation, soil remediation, and solid waste management. In this research, we first explored how vermiculite-to-microbe interactions evolved during sludge-waste mushroom residue co-composting. Vermiculite's addition had a substantial impact on the microbial α and β diversities, significantly changed the microbial community pattern, and strengthened the composting nutrient circulation through the formation of more specialist and generalist species. The microbial community characteristics exhibited common co-networks for resisting composting environment stresses. Vermiculite contributed to enhancing the keystone taxa Proteobacteria and Actinobacteriota and caused the ecological function network to diversify in the warming and maturation phases, with more complexity and tightness in the thermophilic phase (with super-generalist species existing). The enhanced microbial interactions induced by vermiculite possessed a greater capacity to facilitate the metabolisms of carbohydrates and amino acids and cellulolysis, thereby promoting composting humification, and nitrogen retention in the final compost and composting maturity. These findings are helpful for us to understand the biological process mechanisms of the effect of vermiculite additives on composting and contribute to the establishment of a theoretical framework for enhancing the microbial interactions in composting systems by adding vermiculite in practical applications.
作者：
Luo, Yi;He, Youzhi;Wang, Yongfu;Xu, Yuxia;Yang, Li
期刊：
Molecular and Cellular Biochemistry,2024年479(1):171-181 ISSN：0300-8177
通讯作者：
Yi Luo
作者机构：
[He, Youzhi; Yang, Li; Xu, Yuxia; Luo, Yi; Wang, Yongfu] Univ South China, Affiliated Changsha Cent Hosp, Hengyang Med Sch, Dept Spine Surg, 161 Shaoshan South Rd, Changsha 410007, Hunan, Peoples R China.
通讯机构：
[Yi Luo] T;The Affiliated Changsha Central Hospital, Department of Spine Surgery, Hengyang Medical School, University of South China, Changsha, Changsha, People’s Republic of China
摘要：
Intervertebral disc degeneration (IDD) causes pain in the back and neck. This study investigated the role of long non-coding RNA HLA complex group 18 (HCG18) in a cell model of IDD. An IDD model was established by stimulating nucleus pulposus (NP) cells with interleukin (IL)-1 beta. MTT assay was performed to evaluate NP cell viability. The apoptosis was detected by flow cytometry. The expressions of HCG18, microRNA (miR)-495-3p, and follistatin-like protein-1 (FSTL1) were measured by RT-qPCR. The interactions of miR-495-3p with HCG18 and FSTL1 were analyzed by luciferase reporter assay. IL-1 beta stimulation upregulated HCG18 and FSTL1, but downregulated miR-495-3p in NP cells. Silencing of HCG18 or FSTL1, as well as miR-495-3p overexpression in NP cells alleviated IL-1 beta-induced apoptosis and inflammation of NP cells. Both HCG18 and FSTL1 had binding sites for miR-495-3p. Overexpression of FSTL1 abolished the effects of HCG18 silencing on IL-1 beta-induced apoptosis and inflammation. The HCG18/miR-495-3p/FSTL1 axis is essential for IDD development. Therapeutic strategies targeting this axis may be used for IDD treatment.
