作者机构：
[Gong, Junyi; Hou, Jie] Univ South China, Sch Resource Environm & Safety Engn, Hengyang 421001, Peoples R China.
通讯机构：
[Hou, J ] U;Univ South China, Sch Resource Environm & Safety Engn, Hengyang 421001, Peoples R China.
摘要：
Proton-conducting solid oxide fuel cells (H-SOFCs) are expected to be an ideal energy conversion device operating below 600 °C. However, the lack of robust electrodes with high catalytic activity remains a major obstacle to commercialization. Herein, B-site high-entropy modification is first performed on K2NiF4-type oxide to obtain a novel cathode material, La1.2Sr0.8Mn0.2Fe0.2Co0.2Ni0.2Cu0.2O4+δ (LSMFCNC). By harnessing the unique properties of multiple elements, the B-site transition metals in LSMFCNC evolve into various compound states, resulting in crystal structure expansion and a decrease in the bond strength between cations and oxygen ions, thereby promoting the formation of oxygen vacancies and achieving high proton/oxygen diffusion rates with excellent oxygen reduction reaction (ORR) activity. Ultimately, H-SOFCs using the high-entropy LSMFCNC cathode demonstrate an excellent cell performance with power outputs of 1759 mW/cm2 at 700 °C and 1126 mW/cm2 at 600 °C. This performance is superior to La1.2Sr0.8NiO4 (LSNO) and other K2NiF4-type cathodes reported in the literature. The outstanding electrochemical performance and fine operational stability suggest that LSMFCNC could be a potential cathode alternative for low-temperature H-SOFC operation. This study provides a new approach to developing highly active and durable cathodes for H-SOFCs.
摘要：
The effect of 2,2',4,4'-Tetrabromodiphenyl ether (BDE-47), a persistent environmental pollutant commonly used as a flame retardant in various consumer products, on pancreatitis has not been clearly elucidated, although it has been reported to be toxic to the liver, nervous system, and reproductive system. Acute pancreatitis (AP) and chronic pancreatitis (CP) models were induced in this study by intraperitoneal injection of caerulein. The aim was to investigate the impact of BDE-47 on pancreatitis by exposing the animals to acute (1 week) or chronic (8 weeks) doses of BDE-47 (30 mg/kg in the low-concentration group and 100 mg/kg in the high-concentration group). Additionally, BDE-47 was utilized to stimulate mouse bone marrow derived macrophages, pancreatic primary stellate cells, and acinar cells in order to investigate the impact of BDE-47 on pancreatitis. In vivo experiments conducted on mice revealed that chronic exposure to BDE-47, rather than acute exposure, exacerbated the histopathological damage of AP and CP, leading to elevated fibrosis in pancreatic tissue and increased infiltration of inflammatory cells in the pancreas. In vitro experiments showed that BDE-47 can promote the expression of the inflammatory cytokines Tnf-α and Il-6 in M1 macrophages, as well as promote acinar cell apoptosis through the activation of the PERK and JNK pathways via endoplasmic reticulum stress. The findings of this study imply chronic exposure to BDE-47 may exacerbate the progression of both AP and CP by inducing acinar cell apoptosis and dysregulating inflammatory responses.
作者机构：
[Chen, Xun] Univ South China, Sch Nucl Sci & Technol, Hengyang 421001, Peoples R China.;[Huang, Mei; Huang, M] Univ Chinese Acad Sci, Sch Nucl Sci & Technol, Beijing 100049, Peoples R China.
通讯机构：
[Huang, M ] U;Univ Chinese Acad Sci, Sch Nucl Sci & Technol, Beijing 100049, Peoples R China.
摘要：
Based on lattice QCD results of equation of state and baryon number susceptibility at zero baryon chemical potential, and supplemented by machine learning techniques, we construct the analytic form of the holographic black hole metric in the Einstein-Maxwell-Dilaton framework for pure gluon, 2-flavor, and (2 + 1)-flavor systems, respectively. The dilaton potentials solved from Einstein equations are in good agreement with the extended nonconformal DeWolfe-Gubser-Rosen type dilaton potentials fixed by lattice QCD equation of state, which indicates the robustness of the Einstein-Maxwell-Dilaton framework. The predicted critical end point in the (2 + 1)-flavor system is located at (Tc = 0.094 GeV, mu cB = 0.74 GeV), which is close to the results from the realistic Polyakov-Nambu-Jona-Lasinio model, the functional renormalization group, and the holographic model with extended DeWolfe-Gubser-Rosen dilaton potential.
期刊：
Naunyn-Schmiedeberg's Archives of Pharmacology,2024年397(1):627-627 ISSN：0028-1298
通讯作者：
Xiao, Junhui;Li, R
作者机构：
[Shen, Lixian; Tang, Lijing; Li, Rong; Li, R; Xiao, Junhui; Wang, Mei; Xiao, JH; Liu, Meng] Univ South China, Affiliated Hosp 2, Hengyang Med Sch, Dept Pharm, Hengyang 421001, Hunan, Peoples R China.;[Wang, Mei; Liu, Meng] Univ South China, Sch Pharmaceut Sci, Hengyang Med Sch, Dept Pharm & Pharmacol, Hengyang 421000, Hunan, Peoples R China.
通讯机构：
[Li, R ; Xiao, JH] U;Univ South China, Affiliated Hosp 2, Hengyang Med Sch, Dept Pharm, Hengyang 421001, Hunan, Peoples R China.
关键词：
Doxorubicin;Ferroptosis;Liquiritin;Solute carrier family 7 member;Glutathione peroxidase 4
摘要：
Solute carrier family 7 member (SLC7A11) and glutathione peroxidase 4 (GPX4) mediated ferroptosis in doxorubicin-induced cardiotoxicity. Based on the bioinformatics analysis, liquiritin, a flavonoid isolated from the rhizome part of Glycyrrhiza glabra with activities of anti-inflammatory and anti-oxidant, is forecasted to synchronously with ferroptosis-relevant protein. This study aims to investigate the effect of liquiritin on doxorubicin-induced cardiotoxicity and the underlying mechanisms. The C57BL/6 J mice heart or cardiomyocytes were subjected to doxorubicin in vivo or in vitro, which were treated with liquiritin at different dosages. The heart or H9c2 cell cardiotoxicity, relevant protein levels, and ferroptosis were measured by methods of biochemistry, flow cytometry, or Western blot. The mice treated with doxorubicin showed evident cardiotoxicity, concomitant with the downregulation of SLC7A11 and GPX4, and accelerate ferroptosis. Administration of liquiritin could relieve the heart injury, accompanied by restoration of the levels of SLC7A11 and GPX4, and inhibit ferroptosis. And liquiritin ameliorated similar effects in doxorubicin-treated H9c2 cells. Based on these findings, we conclude that liquiritin can protect the doxorubicin-induce mice's cardiotoxicity, and its beneficial effect is related to the reduction of ferroptosis through a mechanism involving the regulation of the SLC7A11/GPX4 pathway.
摘要：
Tantalum is widely used in electronic devices and is crucial for modern society; however, research on the tantalum flow in China was very limited and the scientific evidence for formulating targeted policies was insufficient. Stock and flow of tantalum in China were analyzed here, with its entire life cycle from 2011 to 2019, to reveal the key sectors of tantalum use and its recycling potential. Tantalum production in China was around 1000 t, rising rapidly from 2015 to 2019. Although China was one of the main tantalum producers, its tantalum supply was still heavily relied on other countries, 90% of tantalum concentrate was imported. China consumed 740 t tantalum in 2019, and automobile (224 t/a) and electronics (216 t/a) were the top-two sectors of tantalum use. Generation of post-use tantalum was 520.56 t in 2019, accounting for 42% of the primary production, indicating a great urban mining potential in China. The end-of-life recycling rate was only 10%, which was far below other strategic rare metals in China, due to inadequate recycling system. To promote tantalum recycling, it calls for establishing the source-separating system and "reserve bank" of post-use tantalum, as well as an innovative product design for better recycling tantalum resource.
通讯机构：
[Mao, F ] U;Univ South China, Sch Nucl Sci & Technol, Hengyang 421001, Peoples R China.
摘要：
The electronic and magnetic properties of d0 dilute magnetic semiconductors formed by rock-salt structured magnesium oxide (MgO) doped with C are systematically studied based on first-principles calculations and the Ising model. It is shown that the electronic holes of p states are generated due to the impurity carbon replacing oxygen in MgO, and the magnetic moment of 2 mu B is introduced by each C impurity. The polarization energy and formation energy of C-doped MgO are calculated, and the magnetization energy of C-doped MgO is also calculated which is used to obtain the exchange constant between C impurities. By means of the Ising model, we simulated the magnetization and the susceptibility of the doped system with increasing temperature and obtained the Curie temperature of C-doped MgO. The combination of first-principles and Ising model paves a new way for studying the Curie temperature of dilute magnetic semiconductors.
摘要：
Radioactive strontium ((90)Sr) is considered as one of the most dangerous radionuclides due to its high biochemical toxicity. For the efficient and selective separation of Sr from acidic environments, a novel functional adsorbent CEPA@SBA-15-APTES was prepared in this work through the phosphorylation of amino-modified mesoporous silica with organic content of approximately 20wt%. CEPA@SBA-15-APTES was characterized by TEM, SEM, EDS, TG-DSC, BET, FTIR, and XPS techniques, revealing its characteristics of an ordered hexagonal lattice-like structure and rich functional groups. The experimental results demonstrated that the adsorbent exhibited good adsorption capacity for Sr over a wide acidity range (i.e., from 10(-10) M to 4M HNO(3)). The adsorption equilibriums of Sr by CEPA@SBA-15-APTES in 10(-6) M and 3M HNO(3) solutions were reached within 30 and 5min, respectively, and the adsorption capacities at 318K were 112.6 and 71.8mg/g, respectively. Furthermore, by combining the experimental and characterization results, we found that the adsorption mechanism consisted of ion exchange between Sr(II) and H(+) (in P-OH) in the 10(-6) M HNO(3) solution and coordination between the Sr(II) and oxygen-containing (CO and P=O) functional groups in the 3M HNO(3) solution.
作者机构：
[Wang, YB; Wang, Youbin; Li, Yongkun; Zhou, Jiashun; Zhou, Bingtao] Guangxi Univ, Sch Resources Environm & Mat, State Key Lab Featured Met Mat & Life Cycle Safety, Nanning 530004, Peoples R China.;[Wang, YB; Wang, Youbin; He, Kezhun] Guangxi Alnan Inst Aerosp Transit Aluminum Alloy &, Nanning 530031, Peoples R China.;[Wei, Yuezhou] Univ South China, Sch Nucl Sci & Technol, Hengyang 421001, Peoples R China.
通讯机构：
[Wang, YB ] G;Guangxi Univ, Sch Resources Environm & Mat, State Key Lab Featured Met Mat & Life Cycle Safety, Nanning 530004, Peoples R China.;Guangxi Alnan Inst Aerosp Transit Aluminum Alloy &, Nanning 530031, Peoples R China.
摘要：
In order to enhance the corrosion resistance of 6061 Al, a simple and efficient electrodeposition method for one-step preparation of ZnAlCe layered double hydroxides (LDH) protective film was proposed. The microstructure and corrosion resistance of LDH film were characterized. The results indicated that the presence of Ce promoted the growth of LDH, resulting in denser, thicker ZnAlCe-LDH film, which effectively slowed down the corrosion rate of 6061Al. Particularly, the electrodeposition behavior was investigated in situ using electrochemical quartz crystal microbalance. The electrodeposition of LDH films was divided into rapid deposition of hydroxide and nucleation growth of LDH, and these two processes were described in detail. This work provided novel ideas for the preparation of LDH films on Al alloys.
摘要：
In this paper, we construct three classes of permutation quadrinomials with Niho exponents of the form
$$f(x)=\alpha _0x^r+\alpha _1x^{s_1(p^m-1)+r}+\alpha _2x^{s_2(p^m-1)+r}+\alpha _3x^{s_3(p^m-1)+r}\in \mathbb {F}_{p^{n}}[x]$$
, where p is an odd prime,
$$n=2m $$
is a positive even integer, and
$$(r,s_1,s_2,s_3)=(1,\frac{-1}{p^k-2},1,\frac{p^k-1}{p^k-2})$$
,
$$(1,\frac{p^k+1}{p^k+2},1,\frac{1}{p^k+2})$$
and (3, 1, 2, 3), respectively. The exponents of the first two classes are considered for the first time, and the third class covers all the permutation polynomials proposed by Gupta (Designs Codes and Cryptography 88, 1–17, 2020).
作者机构：
[Wang, Jingyu; Bai, Qinqin; Liang, H; Zheng, Yi; Zhao, Fengxia; Liang, Hao; Yan, Hangli; Wu, Linghao; Niu, Xiangheng] Univ South China, Sch Publ Hlth, Hengyang Med Sch, Dept Publ Hlth Lab Sci, Hengyang 421001, Peoples R China.;[Hu, Hongmei] Hengyang Ctr Dis Control & Prevent, Hengyang 421001, Peoples R China.
通讯机构：
[Liang, H ] U;Univ South China, Sch Publ Hlth, Hengyang Med Sch, Dept Publ Hlth Lab Sci, Hengyang 421001, Peoples R China.
摘要：
The detection of foodborne pathogens is crucial for food hygiene regulation and disease diagnosis. Colorimetry has become one of the main analytical methods in studying foodborne pathogens due to its advantages of visualization, low cost, simple operation, and no complex instrument. However, the low sensitivity limits its applications in early identification and on-site detection for trace analytes. In order to overcome such a limitation, herein we propose a joint strategy featuring dual signal amplification based on the hybridization chain reaction (HCR) and DNA-enhanced peroxidase-like activity of gold nanoparticles (AuNPs) for the sensitive visual detection of Escherichia coli. Target bacteria bound specifically to the aptamer domain in the capture hairpin probe, exposing the trigger domain for HCR and forming the extended double-stranded DNA (dsDNA) structures. The peroxidase-like catalytic capacity of AuNPs can be enhanced significantly by dsDNAs with the sticky ends of dsDNAs being adsorbed on AuNPs and the rigidity of dsDNAs causing the spatial regulation of AuNP concentration. The intensity of the enhancement was linearly related to the number of target bacteria. With the above strategy, the detection limit of our colorimetric method for Escherichia coli was down to 28 CFU mL(-1) within a short analytical time (50 min). This study provides a new perspective for the sensitive and visual detection of early bacterial contamination in foods.
摘要：
The accurate monitors of the electron beams and g -ray fluxes are very important for laser -driven photonuclear studies. The flux -weighted average cross sections and average cross sections per equivalent quantum of 27Al(g, x; x = 2p, p, 3)24Na reactions were measured above the giant dipole resonance region through activation methods. Laser -driven ultra -intense bremsstrahlung grays, generated by laser wakefield accelerated quasi-monoenergetic electrons, were used in the experiment at the 200 TW laser facility of the Compact Laser Plasma Accelerator Laboratory, Peking University. The results demonstrated good agreement with previous works and were compared with theoretical values given by TALYS 1.9 calculations. The experimental cross sections were confirmed to be 2.4 times higher than TALYS 1.9 with default options. This work proved the feasibility of using the 27Al(g, x)24Na reactions as monitors for laser wakefield accelerated electrons and their bremsstrahlung g -ray fluxes.
摘要：
The appearance of recalcitrant organic pollutants such as antibiotics in water bodies has gained a lot of attention owing to their adverse effects on organisms and humans. The current study aims to develop a novel approach to eliminate antibiotic tetracycline (TC) from a synthetic aqueous solution based on the advanced oxidation process triggered by MnSO4-catalyzed NaIO4. Single-factor experiment was performed to observe the impact of pH, NaIO4 concentration, and MnSO4 dosage on TC decomposition, and a three-factor, three-level response surface experiment with TC removal rate as the dependent variable was designed based on the range of factors determined from the single-factor experiment. The single-factor experiment revealed that the ranges of pH, NaIO4 concentration, and MnSO4 dosage need to be further optimized. ANOVA (analysis of variance) results showed that the data from the response surface experiment were consistent with the quadratic model with high R2 (0.9909), and the predicted values were very close to the actual values. After optimization by response surface methodology, the optimal condition obtained was pH = 6.7, [NaIO4] = 0.39 mM, and [MnSO4] = 0.12 mM, corresponding to a TC removal of 96.56%. This optimization condition was fully considered to save the dosage of the high-priced chemical NaIO4. HIGHLIGHTS MnSO4/NaIO4 is a potential advanced oxidative process against antibiotics. The impact of pH, NaIO4 concentration, and MnSO4 dosage on TC decomposition is investigated. Box-Behnken model is adopted to determine the scheme of the experiment. The best experimental condition is optimized using response surface methodology.
作者机构：
[Guo, Yuan; Chen, Fengling; Chen, Zhu] Univ South China, Hengyang Med Sch, Hengyang, Hunan, Peoples R China.;[He, Ziyu; Guo, Yuan; Chen, Fengling; Wang, Chengming] Cent South Univ, Zhuzhou Hosp, Xiangya Sch Med, Dept Cardiovasc Med, Zhuzhou, Hunan, Peoples R China.;[Si, Jiajia; Guo, Yuan; Chen, Zhu] Hunan Univ Technol, Hunan Key Lab Biomed Nanomat & Devices, Zhuzhou, Peoples R China.;[Chen, Zhu; Guo, Yuan; Guo, Y] Univ South China, Hengyang Med Sch, 28 West Changsheng Rd, Hengyang 421001, Hunan, Peoples R China.
通讯机构：
[Chen, Z; Guo, Y ] U;Univ South China, Hengyang Med Sch, 28 West Changsheng Rd, Hengyang 421001, Hunan, Peoples R China.
摘要：
S100A9 is released by immune and tissue cells in response to stressors such as damage, inflammation, and oxidative stress. This leads to the amplification of inflammation, anti‐inflammatory effects, induction of endothelial dysfunction, promotion of vascular microcalcification, as well as stimulation of cell proliferation, autophagy, apoptosis, and death. S100A9 can serve as a biomarker for diagnosis, prediction, and evaluation of cardiovascular diseases, and it is also considered a potential therapeutic target. Abstract Cardiovascular disease (CVD) is a group of diseases that primarily affect the heart or blood vessels, with high disability and mortality rates, posing a serious threat to human health. The causative factors, pathogenesis, and characteristics of common CVD differ, but they all involve common pathological processes such as inflammation, oxidative stress, and fibrosis. S100A9 belongs to the S100 family of calcium‐binding proteins, which are mainly secreted by myeloid cells and bind to the Toll‐like receptor 4 and receptor for advanced glycation end products and is involved in regulating pathological processes such as inflammatory response, fibrosis, vascular calcification, and endothelial barrier function in CVD. The latest research has found that S100A9 is a key biomarker for diagnosing and predicting various CVD. Therefore, this article reviews the latest research progress on the diagnostic and predictive, and therapeutic value of S100A9 in inflammatory‐related CVD such as atherosclerosis, myocardial infarction, and arterial aneurysm and summarizes its molecular mechanisms in the progression of CVD, aiming to explore new predictive methods and to identify potential intervention targets for CVD in clinical practice.
摘要：
Mining can provide abundant resources, but it also causes heavy metal wastewater pollution, which poses a serious threat to aquatic ecosystems. In this study, Cypridopsis vidua was used as the test organism to investigate the single and combined acute toxicity of common heavy metals (such as chromium (Cr) and nickel (Ni)) in mining wastewater. Then, a random forest model (RF model) was used to predict the combined toxicity of chromium and nickel. Single acute toxicity experiments showed that the 96 h-LC(50 )values of the Cr and Ni for Cypridopsis vidua were 0.81 mg/L and 4.3 mg/L, respectively. aCr was more toxic than Ni. Furthermore, the combined acute toxicity experiments demonstrated that the toxicity of Cr-Ni was higher than Ni but lower than Cr. The predicted results of the RF model were highly consistent with the experimental results of the Cr-Ni combined acute toxicity. So, the model has accurate toxicity prediction ability.
摘要：
BackgroundExtra spindle pole bodies-like 1 (ESPL1) is known to play a crucial role in the segregation of sister chromatids during mitosis. Overexpression of ESPL1 is considered to have oncogenic effects in various human cancers. However, the specific biological function of ESPL1 in endometrial cancer (EC) remains unclear.MethodsThe TCGA and GEO databases were utilized to assess the expression of ESPL1 in EC. Immunohistochemistry was utilized to detect separase expression in EC samples. Kaplan-Meier survival analysis and Cox regression analysis were performed to evaluate the diagnostic and prognostic significance of ESPL1 in EC. Gene Set Enrichment Analysis (GSEA) was employed to explore the potential signaling pathway of ESPL1 in EC. Cell proliferation and colony formation ability were analyzed using CCK-8 and colony formation assay.ResultsOur analysis revealed that ESPL1 is significantly upregulated in EC, and its overexpression is associated with advanced clinical characteristics and unfavourable prognostic outcomes. Suppression of ESPL1 attenuated proliferation of EC cell line.ConclusionThe upregulation of ESPL1 is associated with advanced disease and poor prognosis in EC patients. These findings suggest that ESPL1 has the potential to serve as a diagnostic and prognostic biomarker in EC, highlighting its significance in the management of EC patients. The expression of ESPL1 was higher in EC tissue than normal endometrial tissue.ESPL1 could be a potential prognostic marker for EC.
摘要：
We present an atomistic simulation of thermoelectric properties in cove-edged graphene nanoribbons (CGNRs) via the nonequilibrium Green's function. Different from gapless zigzag graphene nanoribbons (ZGNRs), CGNRs exhibit a noticeable bandgap. Such a bandgap can be modulated by varying three structural parameters (namely, the width N, the distance between adjacent coves m, as well as the shortest offset n) of CGNRs, which can give rise to the transition from semiconducting to semi-metallic. Due to the less dispersive phonon bands and the decrease in the number of phonon channels of CGNRs, they are found to have the lower phonon thermal conductance than ZGNRs. Modulation of CGNRs can produce over tenfold improvement of the maximum of ZT compared to ZGNRs. This improvement is due to the promotion of the Seebeck coefficient together with the degradation of the phonon thermal conductance of CGNRs compared to ZGNRs.
关键词：
Efflux pumps;Heavy metal resistance;Secretome
摘要：
Heavy metal-resistant bacteria secrete extracellular proteins (e-PNs). However, the role of e-PNs in heavy metal resistance remains elusive. Here Fourier Transform Infrared Spectroscopy implied that N-H, C=O and NH(2)-R played a crucial role in the adsorption and resistance of Ni(2+) in the model organism Cuprividus pauculus 1490 (C. pauculus). Proteinase K treatment reduced Ni(2+) resistance of C. pauculus underlining the essential role of e-PNs. Further three-dimension excitation-emission matrix fluorescence spectroscopy analysis demonstrated that tryptophan proteins as part of the e-PNs increased significantly with Ni(2+) treatment. Proteomic and quantitative real-time polymerase chain reaction data indicated that major changes were induced in the metabolism of C. pauculus in response to Ni(2+). Among those lipopolysaccharide biosynthesis, general secretion pathways, Ni(2+)-affiliated transporters and multidrug efflux play an essential role in Ni(2+) resistance. Altogether the results provide a conceptual model for comprehending how e-PNs contribute to bacterial resistance and adsorption of Ni(2+).
作者机构：
[Xu, Xiaoding; Xu, Ya; Xu, Lei; Cao, Yuan] Sun Yat Sen Univ, Sun Yat Sen Mem Hosp, Med Res Ctr, Guangdong Prov Key Lab Malignant Tumor Epigenet &, Guangzhou 510120, Peoples R China.;[Xu, Xiaoding; Xu, Ya; Xu, Lei; Cao, Yuan] Sun Yat Sen Univ, Sun Yat Sen Mem Hosp, Guangzhou Key Lab Med Nanomat, Guangzhou 510120, Peoples R China.;[Xu, Xiaoding; Xu, Ya; Xu, Lei; Cao, Yuan] Sun Yat Sen Mem Hosp, Nanhai Translat Innovat Ctr Precis Immunol, Foshan 528200, Peoples R China.;[Li, Rong; Xu, Xiaoding] Univ South China, Affiliated Hosp 2, Hengyang Med Sch, Hengyang 421001, Peoples R China.
通讯机构：
[Xu, XD ] S;Sun Yat Sen Univ, Sun Yat Sen Mem Hosp, Med Res Ctr, Guangdong Prov Key Lab Malignant Tumor Epigenet &, Guangzhou 510120, Peoples R China.;Sun Yat Sen Univ, Sun Yat Sen Mem Hosp, Guangzhou Key Lab Med Nanomat, Guangzhou 510120, Peoples R China.;Sun Yat Sen Mem Hosp, Nanhai Translat Innovat Ctr Precis Immunol, Foshan 528200, Peoples R China.;Univ South China, Affiliated Hosp 2, Hengyang Med Sch, Hengyang 421001, Peoples R China.
关键词：
cancer therapy;delivery;nucleic acid;polymeric nanoparticles;redox responsive
摘要：
Redox‐responsive polymeric nanoparticles (NPs) are widely used for nucleic acid drug delivery and cancer therapy. Herein, the important signaling pathways regulating tumor redox status are summarized and the recent development of redox‐responsive NPs for nucleic acid delivery and cancer therapy is systemically reviewed. The future development of NPs‐mediated nucleic acid delivery and their challenges in clinical translation are also discussed. Abstract Cancer development and progression of cancer are closely associated with the activation of oncogenes and loss of tumor suppressor genes. Nucleic acid drugs (e.g., siRNA, mRNA, and DNA) are widely used for cancer therapy due to their specific ability to regulate the expression of any cancer‐associated genes. However, nucleic acid drugs are negatively charged biomacromolecules that are susceptible to serum nucleases and cannot cross cell membrane. Therefore, specific delivery tools are required to facilitate the intracellular delivery of nucleic acid drugs. In the past few decades, a variety of nanoparticles (NPs) are designed and developed for nucleic acid delivery and cancer therapy. In particular, the polymeric NPs in response to the abnormal redox status in cancer cells have garnered much more attention as their potential in redox‐triggered nanostructure dissociation and rapid intracellular release of nucleic acid drugs. In this review, the important genes or signaling pathways regulating the abnormal redox status in cancer cells are briefly introduced and the recent development of redox‐responsive NPs for nucleic acid delivery and cancer therapy is systemically summarized. The future development of NPs‐mediated nucleic acid delivery and their challenges in clinical translation are also discussed.
通讯机构：
[Yang, SY ] U;Univ South China, Sch Publ Hlth, Hengyang Med Sch, Dept Publ Hlth Lab Sci, Hengyang 421001, Hunan, Peoples R China.;Univ South China, Hunan Key Lab Typ Environm Pollut & Hlth Hazards, Hengyang 421001, Hunan, Peoples R China.
摘要：
Herein, we present a paper-based POCT sensor based on lactate dehydrogenase-mediated alginate gelation combined with visual distance reading and smartphone-assisted colorimetric dual-signal analysis to determine the concentration of (L)-lactate in yogurt samples. In this research, (L)-lactate was transformed into pyruvate by lactate dehydrogenase. Pyruvate then triggered the gelation of a sol mixture, increasing the viscosity (eta(s)) of the mixture, which was shown as a decrease in the diffusion diameter on the paper-based sensor. In addition, protons from pyruvate accelerated the degradation of Rhodamine B, causing color fading of the mixture, which was analyzed using RGB analysis application software. Under optimal experimental conditions, the linear ranges of visual distance reading and smartphone-assisted colorimetric analysis were 0.1-15 mu M and 0.3-15 mu M and the detection limits were 0.03 mu M and 0.07 mu M, respectively. As a proof-of-concept application, we exploited the paper-based sensor to determine the concentration of (L)-lactate in yogurt samples. The results from the dual-signal paper-based sensor were consistent with the ones from HPLC analysis. In short, this study developed a simple, convenient, cost-effective, and feasible method for the quantitative detection of (L)-lactate in real samples.
