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Extracellular proteins enhance Cupriavidus pauculus nickel tolerance and cell aggregate formation

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成果类型:
期刊论文
作者:
Wang, Mingwei;Vollstedt, Christel;Siebels, Bente;Yu, Huang;Wu, Xueling;...
通讯作者:
Zeng, WM;Streit, WR
作者机构:
[Zeng, WM; Liu, Yuandong; Yu, Runlan; Shen, Li; Wang, Mingwei; Wu, Xueling; Li, Jiaokun; Zeng, Weimin] Cent South Univ, Sch Minerals Proc & Bioengn, Changsha 410083, Peoples R China.
[Streit, Wolfgang R.; Streit, WR; Vollstedt, Christel; Wang, Mingwei] Univ Hamburg, Dept Microbiol & Biotechnol, D-22609 Hamburg, Germany.
[Siebels, Bente] Univ Hamburg, Dept Clin Chem & Lab Med, D-20246 Hamburg, Germany.
[Liu, Yuandong; Yu, Runlan; Shen, Li; Wu, Xueling; Li, Jiaokun; Zeng, Weimin] Minist Educ, Key Lab Biomet, Changsha 410083, Peoples R China.
[Yu, Huang] Univ South China, Key Discipline Lab Natl Def Biotechnol Uranium Min, Hengyang 421001, Peoples R China.
通讯机构:
[Zeng, WM ] C
[Streit, WR ] U
Cent South Univ, Sch Minerals Proc & Bioengn, Changsha 410083, Peoples R China.
Univ Hamburg, Dept Microbiol & Biotechnol, D-22609 Hamburg, Germany.
语种:
英文
关键词:
Efflux pumps;Heavy metal resistance;Secretome
期刊:
Bioresource Technology
ISSN:
0960-8524
年:
2024
卷:
393
页码:
130133
基金类别:
National Key Research and Development Program of China Foundation for Innovative Research Groups of the National Natural Science Foundation of China#&#&#2019YFC1803604#&#&#51934009#&#&#52074353#&#&#52274289 Science and Technology Program of Hunan Province National Natural Science Foundation of China Hunan Provincial Natural Science Foundation#&#&#2021NK1040 China Scholarship Council#&#&#202006370228
机构署名:
本校为其他机构
院系归属:
核资源工程学院
摘要:
Heavy metal-resistant bacteria secrete extracellular proteins (e-PNs). However, the role of e-PNs in heavy metal resistance remains elusive. Here Fourier Transform Infrared Spectroscopy implied that N-H, C=O and NH(2)-R played a crucial role in the adsorption and resistance of Ni(2+) in the model organism Cuprividus pauculus 1490 (C. pauculus). Proteinase K treatment reduced Ni(2+) resistance of C. pauculus underlining the essential role of e-PNs. Further three-dimension excitation-emission matrix fluorescence spectroscopy analysis demonstrated that tryptophan proteins as part of the e-PNs inc...

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