摘要：
The electrocatalytic nitrogen reduction reaction (eNRR) under ambient conditions is a promising alternative to the Haber-Bosch process, but one of the primary pending issues for the eNRR is the development of efficient and stable electrocatalysts. Herein, we propose to prepare a ZIF-67-derived nitrogen-doped porous carbon-supported Co9S8 nanocomposite achieving the maximum average of 9.80 mu g h(-1) mg(cat)(-1) NH3 yield and the highest Faradaic efficiency (FE) of 9.89% in 0.1 M Na2SO4. Moreover, Co9S8/NC shows excellent electrocatalytic stability and durability for the eNRR.
通讯机构：
[Jin, YP; Chen, G ; Wang, Y ; Xia, JL] W;[Xia, JL ] F;Wenzhou Med Univ, Affiliated Hosp 1, Dept Hepatobiliary Surg, Wenzhou 325035, Zhejiang, Peoples R China.;Wenzhou Med Univ, Affiliated Hosp 1, Key Lab Diag & Treatment Severe Hepatopancreat Dis, Wenzhou 325035, Zhejiang, Peoples R China.;Wenzhou Med Univ, Sch Publ Hlth & Management, Dept Epidemiol & Biostat, Wenzhou, Zhejiang, Peoples R China.
摘要：
Metastasis causes most cancer-related deaths, and the role and mechanism of periostin (POSTN) in the metastasis of hepatocellular carcinoma (HCC) remain undiscovered. In this study, DEN and HTVi HCC models were performed in hepatic-specific Postn ablation and Postn knock-in mouse to reveal the role of POSTN in HCC metastasis. Furthermore, POSTN was positively correlated with circulating EPCs level and promoted EPC mobilization and tumour infiltration. POSTN also mediated the crosstalk between HCC and EPCs, which promoted metastasis ability and upregulated CD36 expression in HCC through indirect crosstalk. Chemokine arrays further revealed that hepatic-derived POSTN induced elevated CCL2 expression and secretion in EPCs, and CCL2 promoted prometastatic traits in HCC. Mechanistic studies showed that POSTN upregulated CCL2 expression in EPCs via the αvβ3/ILK/NF-κB pathway. CCL2 further induced CD36 expression via the CCR2/STAT3 pathway by directly binding to the promoter region of CD36. Finally, CD36 was verified to have a prometastatic role in vitro and to be correlated with POSTN expression, metastasis and recurrence in HCC in clinical samples. Our findings revealed that crosstalk between HCC and EPCs is mediated by periostin/CCL2/CD36 signalling which promotes HCC metastasis and emphasizes a potential therapeutic strategy for preventing HCC metastasis.
摘要：
In this paper, we presented a novel technique called extended modified auxiliary equation mapping technique, which we used to analyze the combined Chen-Lee-Liu derivative nonlinear Schrodinger equation (MCLL-NLSE) analytically. With the help of three parameters, we were able to use our proposed method to produce newer, broader sets of exact solutions, including bright, periodic, semi half bright, dark, combined, semi half dark, doubly periodic, doubly bright, half bright, and half dark. This is the main distinction between our method and others currently in use. To develop the theoretical fluid dynamics, nonlinear fiber optics, electromagnetism, mathematical physics, bio-mathematics, soliton dynamics, plasma physics, industrial studies, quantum mechanics, nuclear physics and many other natural and physical sciences has been greatly impacted by recently discovered solutions. We have presented the newly discovered solutions in graphs in various dimensions using Mathematica 10.4 to provide a better clear picture of the dynamic properties of the solutions. Additionally, we conducted stability tests on the obtained solutions and presented them as a table.
作者机构：
[Li, Chun-Quan; Tang, Hui-Fang; Qian, Feng-Cui] Univ South China, Hengyang Med Sch, Affiliated Hosp 1, Hengyang 421001, Hunan, Peoples R China.;[Li, Chun-Quan; Tang, Hui-Fang; Qian, Feng-Cui] Univ South China, Hengyang Med Sch, Hunan Prov Key Lab Multiom & Artificial Intelligen, Hengyang 421001, Hunan, Peoples R China.;[Li, Chun-Quan] Univ South China, Hengyang Med Sch, Hunan Prov Maternal & Child Hlth Care Hosp, Natl Hlth Commiss Key Lab Birth Defect Res & Preve, Hengyang 421001, Hunan, Peoples R China.;[Li, Chun-Quan; Qian, Feng-Cui] Univ South China, Sch Basic Med Sci, Dept Biochem & Mol Biol, Hengyang 421001, Hunan, Peoples R China.;[Li, Chun-Quan; Qian, Feng-Cui] Univ South China, Hengyang Med Sch, MOE Key Lab Rare Pediat Dis, Hengyang 421001, Hunan, Peoples R China.
通讯机构：
[Li, CQ ; Tang, HF ; Tang, HF] U;Univ South China, Hengyang Med Sch, Affiliated Hosp 1, Hengyang 421001, Hunan, Peoples R China.;Univ South China, Hengyang Med Sch, Hunan Prov Key Lab Multiom & Artificial Intelligen, Hengyang 421001, Hunan, Peoples R China.;Univ South China, Hengyang Med Sch, Hunan Prov Maternal & Child Hlth Care Hosp, Natl Hlth Commiss Key Lab Birth Defect Res & Preve, Hengyang 421001, Hunan, Peoples R China.;Univ South China, Sch Basic Med Sci, Dept Biochem & Mol Biol, Hengyang 421001, Hunan, Peoples R China.
关键词：
chromatin;genes;software;epigenetics
摘要：
Chromatin accessibility profiles at single cell resolution can reveal cell type-specific regulatory programs, help dissect highly specialized cell functions and trace cell origin and evolution. Accurate cell type assignment is critical for effectively gaining biological and pathological insights, but is difficult in scATAC-seq. Hence, by extensively reviewing the literature, we designed scATAC-Ref (https://bio.liclab.net/scATAC-Ref/), a manually curated scATAC-seq database aimed at providing a comprehensive, high-quality source of chromatin accessibility profiles with known cell labels across broad cell types. Currently, scATAC-Ref comprises 1 694 372 cells with known cell labels, across various biological conditions, >400 cell/tissue types and five species. We used uniform system environment and software parameters to perform comprehensive downstream analysis on these chromatin accessibility profiles with known labels, including gene activity score, TF enrichment score, differential chromatin accessibility regions, pathway/GO term enrichment analysis and co-accessibility interactions. The scATAC-Ref also provided a user-friendly interface to query, browse and visualize cell types of interest, thereby providing a valuable resource for exploring epigenetic regulation in different tissues and cell types.
作者机构：
[Li, Jiangwei; Chen, Yongsheng; Xia, Zhennan] Department of Neurosurgery, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou Medical University, Guangzhou, China;[Liu, Xuanbei; Xie, Jiayu; Ding, Boyun; Lv, Hongzhu; Yang, Bo] Guangdong Provincial Key Laboratory on Brain Function Repair and Regeneration, Department of Neurosurgery, Zhujiang Hospital, The National Key Clinical Specialty, The Neurosurgery Institute of Guangdong Province, The Engineering Technology Research Center of Education Ministry of China, Southern Medical University, Guangzhou, China;[Hong, Enhui] Department of Neurosurgery, Jiu Jiang No.1 People’s Hospital, Jiu Jiang, China;[Jiang, Weiping] Department of Neurosurgery, The First Affiliated Hospital of University of South China, Hengyang, China;[Chen, Yizhao] Department of Neurosurgery, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou Medical University, Guangzhou, China<&wdkj&>Guangdong Provincial Key Laboratory on Brain Function Repair and Regeneration, Department of Neurosurgery, Zhujiang Hospital, The National Key Clinical Specialty, The Neurosurgery Institute of Guangdong Province, The Engineering Technology Research Center of Education Ministry of China, Southern Medical University, Guangzhou, China
通讯机构：
[Yizhao Chen] D;Department of Neurosurgery, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou Medical University, Guangzhou, China<&wdkj&>Guangdong Provincial Key Laboratory on Brain Function Repair and Regeneration, Department of Neurosurgery, Zhujiang Hospital, The National Key Clinical Specialty, The Neurosurgery Institute of Guangdong Province, The Engineering Technology Research Center of Education Ministry of China, Southern Medical University, Guangzhou, China
摘要：
Thioredoxin-reductase 2 (Txnrd2) belongs to the thioredoxin-reductase family of selenoproteins and is a key antioxidant enzyme in mammalian cells to regulate redox homeostasis. Here, we reported that Txnrd2 exerted a major influence in brain damage caused by Intracerebral hemorrhage (ICH) by suppressing endoplasmic reticulum (ER) stress oxidative stress and via Trx2/Prx3 pathway. Furthermore, we demonstrated that pharmacological selenium (Se) rescued the brain damage after ICH by enhancing Txnrd2 expression. Primarily, expression and localization of Txnrd2, Trx2 and Prx3 were determined in collagenase IV-induced ICH model. Txnrd2 was then knocked down using siRNA interference in rats which were found to develop more severe encephaledema and neurological deficits. Mechanistically, we observed that loss of Txnrd2 leads to increased lipid peroxidation levels and ER stress protein expression in neurons and astrocytes. Additionally, it was revealed that Se effectively restored the expression of Txnrd2 in brain and inhibited both the activity of ER stress protein activity and the generation of reactive oxygen species (ROS) by promoting Trx2/Prx3 kilter when administrating sodium selenite in lateral ventricle. This study shed light on the effect of Txnrd2 in regulating oxidative stress and ER stress via Trx2/Prx3 pathway upon ICH and its promising potential as an ICH therapeutic target.
摘要：
BACKGROUND: Opportunistic infections in the central nervous system (CNS) can be a serious threat to people living with HIV. Early aetiological diagnosis and targeted treatment are crucial but difficult. Metagenomic next-generation sequencing (mNGS) has significant advantages over traditional detection methods. However, differences in the cerebrospinal fluid (CSF) microbiome profiles of patients living with and without HIV with suspected CNS infections using mNGS and conventional testing methods have not yet been adequately evaluated. METHODS: We conducted a retrospective cohort study in the first hospital of Changsha between January 2019 and June 2022 to investigate the microbiomes detected using mNGS of the CSF of patients living with and without HIV with suspected CNS infections. The pathogens causing CNS infections were concurrently identified using both mNGS and traditional detection methods. The spectrum of pathogens identified was compared between the two groups. RESULTS: Overall, 173 patients (140 with and 33 without HIV) with suspected CNS infection were enrolled in our study. In total, 106 (75.7%) patients with and 16 (48.5%) patients without HIV tested positive with mNGS (p = 0.002). Among the enrolled patients, 71 (50.7%) with HIV and five (15.2%) without HIV tested positive for two or more pathogens (p < 0.001). Patients with HIV had significantly higher proportions of fungus (20.7% vs. 3.0%, p = 0.016) and DNA virus (59.3% vs. 21.2%, p < 0.001) than those without HIV. Epstein-Barr virus (33.6%) was the most commonly identified potential pathogen in the CSF of patients living with HIV using mNGS, followed by cytomegalovirus (20.7%) and torque teno virus (13.8%). The top three causative pathogens identified in patients without HIV were Streptococcus (18.2%), Epstein-Barr virus (12.1%), and Mycobacterium tuberculosis (9.1%). In total, 113 patients living with HIV were diagnosed as having CNS infections. The rate of pathogen detection in people living with HIV with a CNS infection was significantly higher with mNGS than with conventional methods (93.8% vs. 15.0%, p < 0.001). CONCLUSION: CSF microbiome profiles differ between patients living with and without HIV with suspected CNS infection. mNGS is a powerful tool for the diagnosis of CNS infection among people living with HIV, especially in those with mixed infections.
摘要：
This study proposes and develops a novel ionic concentration gradient electric generator (i‐CGEG) based on the evaporation rate difference of electrolytes. The i‐CGEG with PVA–Na electrolyte achieves a thermovoltage greater than 200 mV and an energy density of 77.94 J m−2 at a temperature of 323 K. Wearable devices and their corresponding cell modules are also developed to recycle body heat. Abstract Constructing concentration differences between anions and cations at the ends of an ionic conductor is an effective strategy in electricity generation for powering wearable devices. Temperature gradient or salinity gradient is the driving force behind such devices. But their corresponding power generation devices are greatly limited in actual application due to their complex structure and harsh application conditions. In this study, a novel ionic concentration gradient electric generator based on the evaporation difference of the electrolyte is proposed. The device can be constructed without the need for semipermeable membranes, and operation does not need to build a temperature difference. As a demonstration, a PVA–Na ionic hydrogel is prepared as an electrolyte for the device and achieved a thermovoltage of more than 200 mV and an energy density of 77.94 J m−2 at 323 K. Besides, the device exhibits the capability to sustain a continuous voltage output for a duration exceeding 1500 min, as well as enabling charging and discharging cycles for 100 iterations. For practical applications, a module comprising 16 sub‐cells is constructed and successfully utilized to directly power a light‐emitting diode. Wearable devices and their corresponding cell modules are also developed to recycle body heat.
作者机构：
[Jia Zhou] The First Affiliated Hospital, Hengyang Medical School, University of South China, Hengyang 421001, China;Authors to whom correspondence should be addressed.;These authors contributed equally to this work.;Department of Urology and Guangdong Key Laboratory of Urology, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou 510230, China;[Yueqi Huang] The First Affiliated Hospital, Hengyang Medical School, University of South China, Hengyang 421001, China<&wdkj&>These authors contributed equally to this work.
通讯机构：
[Guohua Zeng; Qiulin Huang] T;The First Affiliated Hospital, Hengyang Medical School, University of South China, Hengyang 421001, China<&wdkj&>Authors to whom correspondence should be addressed.<&wdkj&>The First Affiliated Hospital, Hengyang Medical School, University of South China, Hengyang 421001, China<&wdkj&>Authors to whom correspondence should be addressed.<&wdkj&>Department of Urology and Guangdong Key Laboratory of Urology, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou 510230, China
摘要：
Primary hyperoxalurias (PHs) are inherited metabolic disorders marked by enzymatic cascade disruption, leading to excessive oxalate production that is subsequently excreted in the urine. Calcium oxalate deposition in the renal tubules and interstitium triggers renal injury, precipitating systemic oxalate build-up and subsequent secondary organ impairment. Recent explorations of novel therapeutic strategies have challenged and necessitated the reassessment of established management frameworks. The execution of diverse clinical trials across various medication classes has provided new insights and knowledge. With the evolution of PH treatments reaching a new milestone, prompt and accurate diagnosis is increasingly critical. Developing early, effective management and treatment plans is essential to improve the long-term quality of life for PH patients.
摘要：
Tuberculosis (TB) caused by Mycobacterium tuberculosis (Mtb) persistently kills nearly 1.5 million lives per year in the world, whereas the only licensed TB vaccine BCG exhibits unsatisfactory efficacy in adults. Taking BCG as a vehicle to express Mtb antigens is a promising way to enhance its efficacy against Mtb infection. In this study, the immune efficacy of recombination BCG (rBCG-ECD003) expressing specific antigens ESAT-6, CFP-10, and nDnaK was evaluated at different time points after immunizing BALB/c mice. The results revealed that rBCG-ECD003 induced multiple Th1 cytokine secretion including IFN-γ, TNF-α, IL-2, and IL-12 when compared to the parental BCG. Under the action of PPD or ECD003, rBCG-ECD003 immunization resulted in a significant increase in the proportion of IL-2(+) and IFN-γ(+)IL-2(+) CD4(+)T cells. Importantly, rBCG-ECD003 induced a stronger long-term humoral immune response without compromising the safety of the parental BCG vaccine. By means of the protective efficacy assay in vitro, rBCG-ECD003 showed a greater capacity to inhibit Mtb growth in the long term. Collectively, these features of rBCG-ECD003 indicate long-term protection and the promising effect of controlling Mtb infection.
摘要：
Cardiovascular disease is the leading cause of death worldwide, and it's of great importance to understand its underlying mechanisms and find new treatments. Sphingosine 1-phosphate (S1P) is an active lipid that exerts its effects through S1P receptors on the cell surface or intracellular signal, and regulates many cellular processes such as cell growth, cell proliferation, cell migration, cell survival, and so on. S1PR modulators are a class of modulators that can interact with S1PR subtypes to activate receptors or block their activity, exerting either agonist or functional antagonist effects. Many studies have shown that S1P plays a protective role in the cardiovascular system and regulates cardiac physiological functions mainly through interaction with cell surface S1P receptors (S1PRs). Therefore, S1PR modulators may play a therapeutic role in cardiovascular diseases. Here, we review five S1PRs and their functions and the progress of S1PR modulators. In addition, we focus on the effects of S1PR modulators on atherosclerosis, myocardial infarction, myocardial ischaemia/reperfusion injury, diabetic cardiovascular diseases, and myocarditis, which may provide valuable insights into potential therapeutic strategies for cardiovascular disease.
作者机构：
[Min, Junxia; Yu, Yingying; Wang, Fudi; Liu, Yutong; Zhou, Jiahui; Yue, Wuyang; Su, Yunxing; Yang, Sisi; Li, Xiaopeng; Min, JX; Sun, Shumin] Zhejiang Univ, Affiliated Hosp 1, Affiliated Hosp 2, Inst Translat Med,Sch Med, Hangzhou 310058, Peoples R China.;[Yu, Yingying; Wang, Fudi; Lin, Zhiting] Univ South China, Affiliated Hosp 1, Sch Publ Hlth, Hengyang Med Sch, Hengyang 421001, Peoples R China.;[Shah, YM; Shah, Yatrik M.; Das, Nupur K.] Univ Michigan, Div Gastroenterol, Internal Med, Ann Arbor, MI 48109 USA.;[Shah, YM; Shah, Yatrik M.; Das, Nupur K.] Univ Michigan, Dept Mol & Integrat Physiol, Ann Arbor, MI 48109 USA.;[Wu, Qian] Zhejiang Univ, Int Inst Med, Sch Med, Yiwu, Zhejiang, Peoples R China.
通讯机构：
[Wang, FD ; Min, JX] Z;[Shah, YM ] U;Zhejiang Univ, Affiliated Hosp 1, Affiliated Hosp 2, Inst Translat Med,Sch Med, Hangzhou 310058, Peoples R China.;Univ South China, Affiliated Hosp 1, Sch Publ Hlth, Hengyang Med Sch, Hengyang 421001, Peoples R China.;Univ Michigan, Div Gastroenterol, Internal Med, Ann Arbor, MI 48109 USA.
关键词：
anemia of inflammation;chemotherapy-induced anemia;FG-4592;HIF;hypoxia;iron-refractory iron deficiency anemia
摘要：
In clinics, hepcidin levels are elevated in various anemia-related conditions, particularly in iron-refractory anemia and in high inflammatory states that suppress iron absorption, which remains an urgent unmet medical need. To identify effective treatment options for various types of iron-refractory anemia, the potential effect of hypoxia and pharmacologically-mimetic drug FG-4592 (Roxadustat) are evaluated, a hypoxia-inducible factor (HIF)-prolyl hydroxylase (PHD) inhibitor, on mouse models of iron-refractory iron-deficiency anemia (IRIDA), anemia of inflammation and 5-fluorouracil-induced chemotherapy-related anemia. The potent protective effects of both hypoxia and FG-4592 on IRIDA as well as other 2 tested mouse cohorts are found. Mechanistically, it is demonstrated that hypoxia or FG-4592 could stabilize duodenal Hif2 alpha, leading to the activation of Fpn transcription regardless of hepcidin levels, which in turn results in increased intestinal iron absorption and the amelioration of hepcidin-activated anemias. Moreover, duodenal Hif2 alpha overexpression fully rescues phenotypes of Tmprss6 knockout mice, and Hif2 alpha knockout in the gut significantly delays the recovery from 5-fluorouracil-induced anemia, which can not be rescued by FG-4592 treatment. Taken together, the findings of this study provide compelling evidence that targeting intestinal hypoxia-related pathways can serve as a potential therapeutic strategy for treating a broad spectrum of anemia, especially iron refractory anemia. In this article, it is demonstrated that targeting the duodenal Hif2 alpha-Fpn axis as a novel strategy to improve refractory hepcidin-activated anemias, including iron-refractory iron-deficiency anemia (IRIDA), inflammatory anemia and chemotherapy-induced anemia, in mice, which provides compelling evidence for further clinical translation.image
通讯机构：
[Wang, H ] U;Univ South China, Sch Nucl Sci & Technol, Hengyang 421001, Peoples R China.;Univ South China, R&D & Modelling Ctr Treatment & Disposal Radioact, Hengyang 421001, Peoples R China.
关键词：
Muscovite;Diffusion;pH;Accessible porosity
摘要：
The migration behavior of Se(IV) and Re(VII) in muscovite was studied by capillary diffusion method and diffusion cell method. The results show that diffusion accelerates when the density decreases and the ionic strength increases. At the same time, pH also significantly affects their migration. The apparent diffusion coefficient ranges from 10–11 to 10–9 m/s2. This work can provide potential applications in deep geological nuclear waste repositories.
期刊：
Molecular and Cellular Biochemistry,2024年:1-17 ISSN：0300-8177
通讯作者：
Zhisheng Jiang
作者机构：
[Yanxia Wang; Zhaoyue Wang; Zhong Ren; Kun Zhou; Shiming Tan; Huiting Liu; Bo Yu; Qian Xu; Yuanqin Zhao] Key Lab for Arteriosclerology of Hunan Province, International Joint Laboratory for Arteriosclerotic Disease Research of Hunan Province, Institute of Cardiovascular Disease, University of South China, Hengyang, 421001, China;[Zhisheng Jiang] Key Lab for Arteriosclerology of Hunan Province, International Joint Laboratory for Arteriosclerotic Disease Research of Hunan Province, Institute of Cardiovascular Disease, University of South China, Hengyang, 421001, China. zsjiang2017@163.com
通讯机构：
[Jiang, Zhisheng] K;Key Lab for Arteriosclerology of Hunan Province, International Joint Laboratory for Arteriosclerotic Disease Research of Hunan Province, Institute of Cardiovascular Disease, University of South China, Hengyang, 421001, China.
关键词：
Ang-1;Angiogenesis;TEK;TSP-1;VEGF
摘要：
Angiopoietin-1 (Ang-1) and Vascular Endothelial Growth Factor (VEGF) are central regulators of angiogenesis and are often inactivated in various cardiovascular diseases. VEGF forms complexes with ETS transcription factor family and exerts its action by downregulating multiple genes. Among the target genes of the VEGF-ETS complex, there are a significant number encoding key angiogenic regulators. Phosphorylation of the VEGF-ETS complex releases transcriptional repression on these angiogenic regulators, thereby promoting their expression. Ang-1 interacts with TEK, and this phosphorylation release can be modulated by the Ang-1-TEK signaling pathway. The Ang-1-TEK pathway participates in the transcriptional activation of VEGF genes. In summary, these elements constitute the Ang-1-TEK-VEGF signaling pathway. Additionally, Ang-1 is activated under hypoxic and inflammatory conditions, leading to an upregulation in the expression of TEK. Elevated TEK levels result in the formation of the VEGF-ETS complex, which, in turn, downregulates the expression of numerous angiogenic genes. Hence, the Ang-1-dependent transcriptional repression is indirect. Reduced expression of many target genes can lead to aberrant angiogenesis. A significant overlap exists between the target genes regulated by Ang-1-TEK-VEGF and those under the control of the Ang-1-TEK-TSP-1 signaling pathway. Mechanistically, this can be explained by the replacement of the VEGF-ETS complex with the TSP-1 transcriptional repression complex at the ETS sites on target gene promoters. Furthermore, VEGF possesses non-classical functions unrelated to ETS and DNA binding. Its supportive role in TSP-1 formation may be exerted through the VEGF-CRL5-VHL-HIF-1α-VH032-TGF-β-TSP-1 axis. This review assesses the regulatory mechanisms of the Ang-1-TEK-VEGF signaling pathway and explores its significant overlap with the Ang-1-TEK-TSP-1 signaling pathway.
期刊：
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY,2024年14:1351540 ISSN：2235-2988
通讯作者：
Gao, H
作者机构：
[Gao, H; Gao, Hong; Tan, Lingling] Univ South China, Affiliated Hosp 2, Hengyang Med Sch, Nursing Dept, Hengyang, Peoples R China.;[Gao, H; Gao, Hong] Ottawa Hosp, Res Inst, Ottawa, ON, Canada.;[Chen, Yahui; Liu, Qiao] Univ South China, Sch Nursing, Hengyang, Peoples R China.;[Li, Genlin; Wang, Xiaolan] Univ South China, Affiliated Hosp 1, Ctr Combinat Obstet & Gynecol & Reprod Med, Hengyang Med Sch, Hengyang, Peoples R China.;[Li, Ting] Univ South China, Affiliated Hosp 2, Hengyang Med Sch, Dept Obstet, Hengyang, Peoples R China.
通讯机构：
[Gao, H ] U;Univ South China, Affiliated Hosp 2, Hengyang Med Sch, Nursing Dept, Hengyang, Peoples R China.;Ottawa Hosp, Res Inst, Ottawa, ON, Canada.
摘要：
Relevant studies increasingly indicate that female reproductive health is confronted with substantial challenges. Emerging research has revealed that the microbiome interacts with the anatomy, histology, and immunity of the female reproductive tract, which are the cornerstone of maintaining female reproductive health and preventing adverse pregnancy outcomes. Currently, the precise mechanisms underlying their interaction and impact on physiological functions of the reproductive tract remain elusive, constituting a prominent area of investigation within the field of female reproductive tract microecology. From this new perspective, we explore the mechanisms of interactions between the microbiome and the anatomy, histology, and immunity of the female reproductive tract, factors that affect the composition of the microbiome in the female reproductive tract, as well as personalized medicine approaches in managing female reproductive tract health based on the microbiome. This study highlights the pivotal role of the female reproductive tract microbiome in maintaining reproductive health and influencing the occurrence of reproductive tract diseases. These findings support the exploration of innovative approaches for the prevention, monitoring and treatment of female reproductive tract diseases based on the microbiome.
作者：
Luo, Yan-Hua;Xie, Li;Li, Jiao-Yang;Xie, Yuan;Li, Man -Qin;...
期刊：
Diabetes, Metabolic Syndrome and Obesity: Targets and Therapy,2024年16:1013-1020 ISSN：1178-7007
通讯作者：
Li Zhou
作者机构：
[Luo, Yan-Hua; Zhou, Li; Li, Man -Qin] Univ South China, Affiliated Hosp 1, Hengyang Med Sch, Dept Metab & Endocrinol, Hengyang 421001, Hunan, Peoples R China.;[Xie, Li] Univ South China, Affiliated Hosp 1, Pediat Med Ctr, Hengyang Med Sch, Hengyang 421001, Hunan, Peoples R China.;[Li, Jiao-Yang] Wuhan Univ, Sch Publ Hlth, Dept Prevent Med, Wuhan 430071, Hubei, Peoples R China.;[Xie, Yuan] Univ South China, Affiliated Hosp 1, Hengyang Med Sch, Dept Radiol, Hengyang 421001, Hunan, Peoples R China.
通讯机构：
[Li Zhou] D;Department of Metabolism and Endocrinology, The First Affiliated Hospital, Hengyang Medical School, University of South China, Hengyang, Hunan, 421001, People’s Republic of China
关键词：
children;mitochondrial-derived peptides;MOTS-C;obese;vascular endothelial function
期刊：
Computers in biology and medicine,2024年173:108245 ISSN：0010-4825
通讯作者：
Cheng, Hao;Dai, Weiwei
作者机构：
[Hu, Min; Li, Maoyan; Tan, Qian; Cao, Danmin] Aier Institute of Digital Ophthalmology & Visual Science, Changsha Aier Eye Hospital, Changsha, China;[Cao, Danmin] Department of Ophthalmology, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, China;[Cao, Danmin] Aier Eye Hospital of Wuhan University, Wuhan, China;[Hu, Min] Institute of Computing Technology, Chinese Academy of Sciences, Beijing, China;[Zhi, Danlin] The First Affiliated Hospital of University of South China, Hengyang, China
通讯机构：
[Cheng, Hao] D;[Dai, Weiwei] A;Department of Ophthalmology, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, China. Electronic address:;Aier Institute of Digital Ophthalmology & Visual Science, Changsha Aier Eye Hospital, Changsha, China. Electronic address:
摘要：
PURPOSE: This study aimed to evaluate and optimize intraocular lens (IOL) power selection for cataract patients with high axial myopia receiving trifocal IOLs. DESIGN: A multi-center, retrospective observational case series was conducted. Patients having an axial length ≥26mm and undergoing cataract surgery with trifocal IOL implanted were studied. METHODS: Preoperative biometric and postoperative outcome data from 139 eyes were collected to train and test various machine learning (ML) models (support vector machine, linear regression, and stacking regressor) using five-fold cross-validation. The models' performance was further validated externally using data from 48 eyes enrolled from other hospitals. Performance of seven IOL calculation formulas (BUII, Kane, EVO, K6, DGS, Holladay I, and SRK/T) were examined with and without ML models. RESULTS: The results of cross-validation revealed improvements across all IOL calculation formulas, especially for K6 and Holladay I. The model increased the percentage of eyes with a prediction error (PE) within ±0.50 D from 71.94% to 79.14% for K6, and from 35.25% to 51.80% for Holladay I. In external validation involving 48 patients from other centers, six out of seven formulas demonstrated a reduction in the mean absolute error (MAE). K6's PE within ±0.50 D improved from 62.50% to 77.08%, and Holladay I from 16.67% to 58.33%. CONCLUSIONS: In this study, we conducted a comprehensive evaluation of seven IOL power calculation formulas in high axial myopia cases and explored the effectiveness of the Stacking Regressor model in augmenting their accuracy. Of these formulas, K6 and Holladay I exhibited the most significant improvements, suggesting that integrating ML may have varying levels of effectiveness across different formulas but holds substantial promise in improving the predictability of IOL power calculations in patients with long eyes.
摘要：
Radiation encephalopathy (RE) refers to radiation-induced brain necrosis and is a life-threatening complication in patients with nasopharyngeal carcinoma (NPC) after radiotherapy (RT), and radiation-induced pre-symptomatic glymphatic alterations have not yet been investigated. We used diffusion tensor image analysis along the perivascular space (DTI-ALPS) index to examine the pre-symptomatic glymphatic alterations in NPC patients following RT. A total of 109 patients with NPC consisted of Pre-RT (n = 35) and Post-RT (n = 74) cohorts were included. The post-RT NPC patients, with normal-appearing brain structure at the time of MRI, were further divided into Post-RT-RE- (n = 58) and Post-RT-RE+ (n = 16) subgroups based on the detection of RE in follow-up. We observed lower DTI-ALPS (left) index, DTI-ALPS (right) index and DTI-ALPS (whole brain) index in post-RT patients than that in pre-RT patients (p < 0.05). We further found that post-RT-RE+ patients demonstrated significantly lower DTI-ALPS (right) (p = 0.013), DTI-ALPS (whole brain) (p = 0.011) and marginally lower DTI-ALPS (left) (p = 0.07) than Post-RT (non-RE) patients. Significant negative correlations were observed between the maximum dosage of radiation-treatment (MDRT) and DTI-ALPS (left) index (p = 0.003) as well as DTI-ALPS (whole brain) index (p = 0.004). Receiver operating characteristic (ROC) curve analysis showed that DTI-ALPS (whole brain) index exhibited good performance (AUC = 0.706) in identifying patients more likely developing RE. We concluded that glympathic function was impaired in NPC patients following RT and DTI-ALPS index may serve as a novel imaging biomarker for diagnosis of RE.
作者机构：
[He, Bisha; Hu, Yibao; Cao, Qian; Li, Yue; Tang, Yun; Cao, Ting; Zhou, Xiangping] Department of Clinical Laboratory Medicine, Institution of microbiology and infectious diseases, The First Affiliated Hospital, Hengyang Medical School, University of South China,Hengyang,Hunan 421001,China;[Liu, Shuangquan] Department of Clinical Laboratory Medicine, Institution of microbiology and infectious diseases, The First Affiliated Hospital, Hengyang Medical School, University of South China,Hengyang,Hunan 421001,China. Electronic address: dantelliu@163.com
通讯机构：
[Shuangquan Liu] D;Department of Clinical Laboratory Medicine, Institution of microbiology and infectious diseases, The First Affiliated Hospital, Hengyang Medical School, University of South China, Hengyang, Hunan 421001, China
关键词：
Angiogenesis;Ferroptosis;Molecular mechanism;Unfolded protein response
摘要：
Angiogenesis is the growth of new blood vessels on preexisting ones. It is the outcome of a multifactorial effect involving several cells, which can be brought on by different stress reactions.The accumulation of unfolded proteins in the endoplasmic reticulum occurs when cells are stressed due to environmental changes, where physical or chemical stimuli induce endoplasmic reticulum stress, thereby activating the unfolded protein response (UPR), a homeostasis response designed to re-establish protein balance. Ferroptosis is a planned death of lipid peroxidation and anomalies in metabolism that is dependent on iron. Large concentrations of iron ions accumulate there, along with high concentrations of lipid peroxides and reactive oxygen species, all of which can contribute to the development of several diseases. Through the production of growth factors, adhesion factors, and inflammatory factors that trigger the start of angiogenesis, both UPR and Ferroptosis can be implicated in angiogenesis.To set the stage for further research on angiogenesis, this work concentrated on the effects of Ferroptosis and UPR on angiogenesis, respectively.
