作者机构：
[Liu, Jun; Zhang, Tao] Univ South China, Affiliated Hosp 2, Dept Urol, Hengyang, Peoples R China.;[He, Pingping] Univ South China, Inst Cardiovasc Dis, Coll Med, Dept Physiol, Hengyang, Peoples R China.;[Chen, Xin; Li, E.] Hunan Tradit Chinese Med Coll, Zhuzhou, Peoples R China.;[Peng, Zhe; Wang, Bo; Liao, Huiying; Fu, Wan] Univ South China, First Affiliated Hosp, Hengyang, Peoples R China.;[He, Pingping] Univ S China, Sch Nursing, Hengyang, Peoples R China.
会议名称：
International Conference on Biological Engineering and Biomedical (BEAB)
会议时间：
JAN 10-12, 2014
会议地点：
Yichang, PEOPLES R CHINA
会议主办单位：
[He, Pingping] Univ South China, Inst Cardiovasc Dis, Coll Med, Dept Physiol, Hengyang, Peoples R China.^[Zhang, Tao;Liu, Jun] Univ South China, Affiliated Hosp 2, Dept Urol, Hengyang, Peoples R China.^[Chen, Xin;Li, E.] Hunan Tradit Chinese Med Coll, Zhuzhou, Peoples R China.^[Wang, Bo;Liao, Huiying;Fu, Wan;Peng, Zhe] Univ South China, First Affiliated Hosp, Hengyang, Peoples R China.^[He, Pingping] Univ S China, Sch Nursing, Hengyang, Peoples R China.
摘要：
Object: To observe the influence of silencing the expression of YB-1 gene by RNA interference cell on proliferation and migration of prostate cancer. Methods: The YB-1 siRNA was transfected into PC-3 cells at logarithmic growth by liposome, expression levels of YB-1 mRNA and protein were detected by reverse transcription-polymerase chain reaction (RT-PCR) and Wesretn-blot, proliferation and invasion of PC-3 cell was detected by MTT assay and Transwell experiments. Results: After transfection for 48 h, compared with PC-3 cells non-transfected with plasmid, YB-1 mRNA levels and YB-1 protein levels in PC-3 cells transfected with recombinant plasmid pGenesil-1-YB-1-1 and recombinant plasmid pGenesil-1-YB-1-2 were significantly decreased (P< 0.05). YB-1 mRNA inhibition rates in PC-3 cells transfected with recombinant plasmid pGenesil-1-YB-1-1 and recombinant plasmid pGenesil-1-YB-1-2 were 36.23% and 39.42%, while YB-1 protein inhibition rates were 41.56% and55.33%. MTT results showed that the proliferation of PC-3 cells transfected with recombinant plasmid pGenesil-1YB- 1-1 and recombinant plasmid pGenesil-1-YB-1-2 was significantly decreased when compared with PC-3 cells non-transfected with plasmid (P< 0.05). Transwell migration experiments showed that the number of cells go through the membrane in PC-3 cells non-transfected, PC-3 cells transfected with pGenesil-1-YB-NC, PC-3 cells transfected with recombinant plasmid pGenesil-1-YB-1-1 and PC-3 cells transfected with recombinant plasmid pGenesil-1-YB-1-2, were(123.71+/-13.25),(135.73+/-14.18),(67.73+/-8.82) and (51.73+/-6.75) respectively (P<0.05). The migration of PC-3 cells was reduced by downregulating the expression of YB-1, also the invasiveness of PC-3 cells vitro was inhibited. Conclusions: cell proliferation and migration of prostate cancer was inhibited by RNA interference silencing the expression of YB-1 gene.
摘要：
Lipoprotein lipase (LPL) is a key enzyme in lipid metabolism and responsible for catalyzing lipolysis of triglycerides in lipoproteins. LPL is produced mainly in adipose tissue, skeletal and heart muscle, as well as in macrophage and other tissues. After synthesized, it is secreted and translocated to the vascular lumen. LPL expression and activity are regulated by a variety of factors, such as transcription factors, interactive proteins and nutritional state through complicated mechanisms. LPL with different distributions may exert distinct functions and have diverse roles in human health and disease with close association with atherosclerosis. It may pose a pro-atherogenic or an anti-atherogenic effect depending on its locations. In this review, we will discuss its gene, protein, synthesis, transportation and biological functions, and then focus on its regulation and relationship with atherosclerosis and potential underlying mechanisms. The goal of this review is to provide basic information and novel insight for further studies and therapeutic targets. (C) 2014 Elsevier Ireland Ltd. All rights reserved.
摘要：
ATP-binding cassette transporter A1 (ABCA1) is critical in exporting cholesterol from macrophages and plays a protective role in the development of atherosclerosis. The purpose of this study was to investigate the effects of betulinic acid (BA), a pentacyclic triterpenoid, on ABCA1 expression and cholesterol efflux, and to further determine the underlying mechanism. BA promoted ABCA1 expression and cholesterol efflux, decreased cellular cholesterol and cholesterol ester content in LPS-treated macrophages. Furthermore, we found that BA promoted ABCA1 expression via down-regulation of miR-33s. The inhibition of LPS-induced NF-kappa B activation further decreased miR-33s expression and enhanced ABCA1 expression and cholesterol efflux when compared with BA only treatment. In addition, BA suppressed I kappa B phosphorylation, p65 phosphorylation and nuclear translocation, and the transcription of NF-kappa B-dependent related gene. Moreover, BA reduced atherosclerotic lesion size, miR-33s levels and NF-kappa B activation, and promoted ABCA1 expression in apoE(-/-) mice. Taken together, these results reveal a novel mechanism for the BA-mediated ABCA1 expression, which may provide new insights for developing strategies for modulating vascular inflammation and atherosclerosis.
