MiRNA-21 reverses high glucose and high insulin induced insulin resistance in 3T3-L1 adipocytes through targeting phosphatase and tensin homologue
作者:
Ling, H. -y.;Hu, B.;Hu, X. -b.;Zhong, J.;Feng, S. -d.;...
期刊:
EXPERIMENTAL AND CLINICAL ENDOCRINOLOGY & DIABETES ,2012年120(9):553-559 ISSN:0947-7349
通讯作者:
Liao, D. -f.
作者机构:
[Ling, H. -y.; Hu, B.] Univ S China, Sch Med, Dept Physiol, Hengyang, Peoples R China.;[Liao, D. -f.] Hunan Univ Chinese Med, State Key Lab Chinese Med Powder & Med Innovat Hu, Div Stem Cell Regulat & Applicat, Changsha 410208, Hunan, Peoples R China.;[Ling, H. -y.] Univ S China, Ctr Basic Med Postdoctoral Studies, Hengyang, Peoples R China.;[Hu, X. -b.] Univ S China, Sch Life Sci & Technol, Dept Biochem & Mol Biol, Hengyang, Peoples R China.;[Wen, G. -b.; Zhong, J.] Univ S China, Affiliated Hosp 1, Inst Clin Res, Hengyang, Peoples R China.
通讯机构:
[Liao, D. -f.] H;Hunan Univ Chinese Med, State Key Lab Chinese Med Powder & Med Innovat Hu, Div Stem Cell Regulat & Applicat, Changsha 410208, Hunan, Peoples R China.
关键词:
miR-21;insulin resistance;PTEN;AKT
摘要:
Aims/hypothesis: Our previous study showed there was a change of microRNA (miRNA) expression profile, and miR-21 was significantly down regulated in insulin-resistant adipocytes (IR-adipocytes). Phosphatase and tensin homologs deleted on chromosome 10 (PTEN), a negative regulator of the phosphatidylinositol 3-kinase (PI3K)/AKT pathway, was identified to be a target gene of miR-21, which suggested miR-21 might be associated with insulin resistance (IR) or diabetes. However, it is not known whether miR-21 play any role in the development of IR in 3T3-L1 adipocytes. Methods: Normal adipocytes and adipocytes transfected with pre-miR-21(pmiR-21) or negative control (pNeg) were treated with high glucose and high insulin for 24h, insulin-stimulated glucose uptake was determined by 2-Deoxyglucose transport assay, miR-21 expression level was measured by using quantitative real-time RT-PCR (qRT-PCR). The protein expression levels of PTEN, Akt, phospho-Akt (Ser473), IRβ, GSK3β, phospho-GSK3β (Ser9) and GLUT4 were detected by western blotting assay. Results: We further confirmed that miR-21 was down regulated in IR-adipocytes by qRT-PCR. Over-expression of miR-21 significantly increased insulin-induced glucose uptake and decreased PTEN protein expression, while it had no significant effect on PTEN mRNA expression in IR-adipocytes. Moreover, over-expressing miR-21 significantly increased insulin-induced phosphorylation of AKT (Ser473), GSK3β (Ser9) and the translocation of glucose transporter 4 (GLUT4) in IR-adipocytes. Conclusions: In this study, our data demonstrate that miR-21 reverses high glucose and high insulin induced IR in 3T3-L1 adipocytes, possibly through modulating the PTEN-AKT pathway, and miR-21 may be a new therapeutic target for metabolic diseases such as T2DM and obesity. © Georg Thieme Verlag KG Stuttgart · New York.
语种:
英文
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MicroRNA-375 promotes 3T3-L1 adipocyte differentiation through modulation of extracellular signal-regulated kinase signalling
作者:
Ling, Hong-Yan;Wen, Ge-Bo;Feng, Shui-Dong;Tuo, Qin-Hui;Ou, He-Sheng;...
期刊:
Clinical and Experimental Pharmacology and Physiology ,2011年38(4):239-246 ISSN:0305-1870
通讯作者:
Zhang, Liang
作者机构:
[Ling, Hong-Yan] Univ S China, Sch Med, Dept Physiol, Hengyang, Peoples R China.;[Wen, Ge-Bo; Ling, Hong-Yan] Univ S China, Affiliated Hosp 1, Inst Clin Res, Hengyang, Peoples R China.;[Feng, Shui-Dong] Univ S China, Sch Publ Hlth, Dept Epidemiol, Hengyang, Peoples R China.;[Gao, Zhi-Ping; Zhu, Bing-Yang; Ou, He-Sheng; Tuo, Qin-Hui; Liao, Duan-Fang] Univ S China, Inst Pharm & Pharmacol, Key Lab Pharmacoprote Hunan Prov, Hengyang, Peoples R China.;[Liao, Duan-Fang] Hunan Univ Chinese Med, Sch Pharm, Dept Tradit Chinese Diagnost, Changsha, Hunan, Peoples R China.
通讯机构:
[Zhang, Liang] P;Palmer Coll Chiropract Florida, Palmer Ctr Chiropract Res, Lab Cell & Mol Biol, 4705 S Clyde Morris Blvd, Port Orange, FL 32129 USA.
关键词:
3T3-L1 adipocytes;Differentiation;Extracellular signal-regulated kinases 1/2;MicroRNA-375;Obesity
摘要:
1. Adipocyte hypertrophy and hyperplasia are important processes in the development of obesity. To understand obesity and its associated diseases, it is important to elucidate the molecular mechanisms governing adipogenesis. MicroRNA-375 has been shown to inhibit differentiation of neurites, and participate in the regulation of insulin secretion and blood homeostasis. However, it is unknown whether miR-375 plays a role in adipocyte differentiation. 2. To investigate the role of miR-375 in adipocyte differentiation, we compared the miR-375 expression level between 3T3-L1 pre-adipocytes and adipocytes using miRNA microarray and quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR) analysis. Furthermore, we evaluated the effects of overexpression or inhibition of miR-375 on 3T3-L1 adipocyte differentiation. 3. In the present study, we found that miR-375 expression was increased after induction of adipogenic differentiation. Overexpression of miR-375 enhanced 3T3-L1 adipocyte differentiation, as evidenced by its ability to increase mRNA levels of both CCAAT/enhancer binding protein-α (C/EBPα) and peroxisome proliferator-activated receptor-γ (PPARγ2), and induction of adipocyte fatty acid-binding protein (aP2) and triglyceride (TG) accumulation. Furthermore, we found overexpression of miR-375 suppressed phosphorylation levels of extracellular signal-regulated kinases 1/2 (ERK1/2). In contrast, anti-miR-375 increased ERK1/2 phosphorylation levels and inhibited mRNA expression of C/EBPα, PPARγ2 and aP2 in 3T3-L1 adipocyte, accompanied by decreased adipocyte differentiation. 4. Taken together, these data suggest that miR-375 promotes 3T3-L1 adipocyte differentiation, possibly through modulating the ERK-PPARγ2-aP2 pathway. © 2011 The Authors. Clinical and Experimental Pharmacology and Physiology © 2011 Blackwell Publishing Asia Pty Ltd.
语种:
英文
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衡阳市某大学学生结核病防治知识信念和行为调查
作者:
奉水东;杨绍群;凌宏艳
期刊:
中国校医 ,2011年25(11):815-817 ISSN:1001-7062
作者机构:
南华大学公共卫生学院 湖南 衡阳 421001;南华大学公共卫生学院2005级预防专业;南华大学医学院生理学教研室;[凌宏艳; 奉水东; 杨绍群] 南华大学
关键词:
成年人;学生;结核;健康知识;态度;实践
摘要:
目的 了解衡阳市某大学学生结核病防治知识、信念和行为的状况. 方法 采用分层随机整群抽样的方法对某大学大一和大三的559名学生进行匿名调查. 结果 学生对结核病防治知识核心信息的总知晓率为43.45%;两条核心信息(结核病的传染性、结核病人应到专门机构检查治疗)的单一知晓率超过50%;学生获取结核病相关知识的途径主要为“听别人说的”(40.8%),其次为电视(25.4%)和报纸(14.8%);学生对结核病的态度和行为正确持有率较低,大三年级主动了解过结核知识的学生高于大一年级,正确持有率为31.3%;有56.7%的学生曾经拿过结核病防治的宣传资料. 结论大学生对结核病防治知识的知晓率较低,对结核病信念和行为的正确持有率较低,有必要在大学生中广泛开展结核病防治相关的健康教育.
语种:
中文
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Detecting overexpression level of HER2 gene in NSCLC by real-time quantitative PCR and the 2[-delta delta C(T)] method
作者:
奉水东;谭红专;凌宏艳;袁秀琴
期刊:
中国肺癌杂志 ,2011年14(12):938-942 ISSN:1009-3419
通讯作者:
Feng, S.
作者机构:
[袁秀琴; 奉水东] Department of social medicine and health services management, School of public health, University of south china, Hengyang 421001, China;[谭红专] Department of epidemiology and health statistics, School of public health, Central south university, Changsha 410087, China;[凌宏艳] Department of physiology, School of medicine, University of south china, Hengyang 421001, China
通讯机构:
[Feng, S.] D;Department of social medicine and health services management, School of public health, University of south china, China
关键词:
实时定量PCR;HER2基因;肺肿瘤;表达
摘要:
背景与目的正确评价HER2基因表达的状况对于肿瘤的施治具有重要的指导意义。以往的评价大多基于免疫组织化学法,但结果变异性大。本研究旨在探讨实时定量PCR 2~(-△△Ct)法检测非小细胞肺癌(non-small celllung cancer,NSCLC)HER2基因表达的可行性。方法采用实时定量PCR 2~(-△△Ct)法同时检测212例肺癌组织和与其匹配的非肿瘤组织HER2基因的表达,评价过表达水平。结果肺癌组织中HER2基因的表达水平高于非肿瘤组织(T=67,P〈0.05),HER2基因的过表达率为34.0%。结论实时定量PCR2~(-△△Ct)法用于检测NSCLCHER2基因的表达是可行的
语种:
中文
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可疑环境暴露与非小细胞肺癌EGFR基因突变的流行病学研究
作者:
奉水东;谭红专;凌宏艳;陈梦施
作者机构:
[奉水东; 谭红专; 凌宏艳; 陈梦施] 南华大学公共卫生学院流行病与卫生统计学教研室;[奉水东; 谭红专; 凌宏艳; 陈梦施] 中南大学公共卫生学院流行病与卫生统计学教研室;[奉水东; 谭红专; 凌宏艳; 陈梦施] 南华大学医学院生理学教研室
会议名称:
2011年环境污染与大众健康学术会议
会议时间:
2011-09-16
会议地点:
中国湖北武汉
关键词:
非小细胞肺癌;EGFR基因;突变;环境因素;流行病学
摘要:
目的:探讨可疑环境暴露与非小细胞肺癌(non-small cell lung cancer,NSCLC)EGFR基因突变有无关联。方法:选择2006年6月至2007年6月在湖南省肿瘤医院、中南大学湘雅医院和中南大学湘雅二医院三所大型医院收治住院并通过病理组织学诊断为NSCLC的212例手术病人作为研究对象,应用流行病学现况研究的方法,通过查阅病历、现场询问、收集肿瘤组织和DNA测序等手段获得有关环
语种:
中文
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大学生抑郁症与ABO血型的关系
作者:
奉水东;王冠琳;凌宏艳;周雪
期刊:
中国学校卫生 ,2011年32(12):1517 ISSN:1000-9817
作者机构:
南华大学公共卫生学院社会医学与卫生事业管理教研室;南华大学公共卫生学院2006级预防医学01班;南华大学医学院生理学教研室;[凌宏艳; 奉水东; 周雪; 王冠琳] 南华大学
关键词:
抑郁;ABO血型系统;对比研究;学生
摘要:
抑郁症是广义的精神疾病,主要表现为情绪低落、兴趣减低、悲观、不适感等相关症状,严重者可产生自杀意念和行为[1].在校大学生属于特殊的群体,生理和心理虽已高度发展但不完善,具有较为敏感、脆弱的特点,因而极易受到侵扰,是抑郁症的高发人群,应该引起学校和家庭的注意.有研究显示,9.1%的大学生有自杀意念,而其最大的危险因素是抑郁症[2-3].研究表明,抑郁症和人格、遗传等因素有关[4].本研究旨在探讨大学生抑郁症和ABO血型之间的关系,为高校开展心理卫生保健工作提供参考依据.
语种:
中文
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3T3-L1脂肪细胞与胰岛素抵抗脂肪细胞miRNAs表达谱的差异性分析
作者:
凌宏艳;胡弼;庹勤慧;刘刚;奉水东;...
期刊:
中国现代医学杂志 ,2011年21(31):3856-3860 ISSN:1005-8982
作者机构:
[凌宏艳; 胡弼] 南华大学医学院生理学教研室;[庹勤慧; 刘刚; 朱炳阳] 南华大学药物药理研究所药理学教研室;[奉水东] 南华大学公共卫生学院流行病教研室;[廖端芳] 湖南中医药科大学药学院
关键词:
miRNAs表达谱;3T3-L1脂肪细胞;胰岛素抵抗;差异性分析
摘要:
目的 探讨正常3T3-L1脂肪细胞和胰岛素抵抗(IR)脂肪细胞微小RNA(miRNAs)的差异性表达,并进行初步分析.方法 采用高糖(25 mmol/L葡萄糖)/高胰岛素液(1μmol/L)处理3T3-L1脂肪细胞24h,通过葡萄糖摄取实验判断IR脂肪细胞模型的建立;接着通过miRNA芯片技术,检测脂肪细胞和IR脂肪细胞中miRNAs的表达,并对其分析;随后借助生物信息学方法,对显著变化的2个miRNAs (miR-320和miR-21)寻找并筛检其可能调控的靶基因.结果 共获得79个IR相关miRNAs(29个低表达,50个高表达,其中miR-320显著上调,miR-21显著下调),且筛选出与IR或糖尿病相关的靶基因共13个.结论 获得了3T3-L1脂肪细胞和IR脂肪细胞miRNAs的差异性表达谱,为进一步研究这些miRNAs在IR和糖尿病的发病机制中的作用奠定了基础.
语种:
中文
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Prediction of posttraumatic stress disorder among adults in flood district
作者:
Peng Huang;Tan, Hongzhuan* ;Liu, Aizhong;Feng, Shuidong;Chen, Mengshi
期刊:
BMC PUBLIC HEALTH ,2010年10(1):207 ISSN:1471-2458
通讯作者:
Tan, Hongzhuan
作者机构:
[Peng Huang; Tan, Hongzhuan; Liu, Aizhong; Chen, Mengshi] Cent S Univ, Sch Publ Hlth, Changsha 410078, Hunan, Peoples R China.;[Peng Huang] Nanchang Univ, Sch Publ Hlth, Nanchang 330006, Jiangxi, Peoples R China.;[Feng, Shuidong] Univ S China, Sch Publ Hlth, Hengyang 421001, Hunan, Peoples R China.;[Tan, Hongzhuan] Cent S Univ, Sch Publ Hlth, Xiangya Rd 110, Changsha 410078, Hunan, Peoples R China.
通讯机构:
[Tan, Hongzhuan] C;Cent S Univ, Sch Publ Hlth, Xiangya Rd 110, Changsha 410078, Hunan, Peoples R China.
关键词:
Positive Predictive Value;Risk Score;Negative Predictive Value;Posttraumatic Stress Disorder;Flash Flood
摘要:
Background. Flood is one of the most common and severe forms of natural disasters. Posttraumatic stress disorder (PTSD) is a common disorder among victims of various disasters including flood. Early prediction for PTSD could benefit the prevention and treatment of PTSD. This study aimed to establish a prediction model for the occurrence of PTSD among adults in flood districts. Methods. A cross-sectional survey was carried out in 2000 among individuals who were affected by the 1998 floods in Hunan, China. Multi-stage sampling was used to select subjects from the flood-affected areas. Data was collected through face-to-face interviews using a questionnaire. PTSD was diagnosed according to DSM-IV criteria. Study subjects were randomly divided into two groups: group 1 was used to establish the prediction model and group 2 was used to validate the model. We first used the logistic regression analysis to select predictive variables and then established a risk score predictive model. The validity of model was evaluated by using the model in group 2 and in all subjects. The area under the receiver operation characteristic (ROC) curve was calculated to evaluate the accuracy of the prediction model. Results. A total of 2336 (9.2%) subjects were diagnosed as probable PTSD-positive individuals among a total of 25,478 study subjects. Seven independent predictive factors (age, gender, education, type of flood, severity of flood, flood experience, and the mental status before flood) were identified as key variables in a risk score model. The area under the ROC curve for the model was 0.853 in the validation data. The sensitivity, specificity, positive predictive value (PPV) and negative predictive value (NPV) of this risk score model were 84.0%, 72.2%, 23.4%, and 97.8%, respectively, at a cut-off value of 67.5 in the validation data. Conclusions. A simple risk score model can be used to predict PTSD among victims of flood. © 2010 Huang et al; licensee BioMed Central Ltd.
语种:
英文
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衡阳市大学生性知识水平和性教育需求调查
作者:
奉水东;陈敏时;陈新;凌宏艳;龙理良;...
期刊:
中国校医 ,2009年23(1):49-50 ISSN:1001-7062
作者机构:
南华大学公共卫生学院流行病学教研室,湖南,衡阳,421001;南华大学生理学教研室;南华大学2000级预防班
关键词:
性教育;学生保健服务
摘要:
目的 了解大学生性知识水平和性教育需求. 方法 采用分层整群抽样方法对衡阳市南华大学的432名在校大学生进行匿名调查. 结果 大学生对性知识"了解很多"的为4.4%,"基本上了解"的为35.1%;大学生现有的性知识主要来源于书刊、报纸和杂志(41.9%);希望在大学受到系统性教育的大学生为86.3%(男生82.9%,女生91.4%);大学生认为较好的性教育方式是设立专门的课程(40.5%)和专题讲座(30.6%). 结论 大学生性知识比较缺乏,渴求通过多样化方式在大学受到系统的性健康教育.
语种:
中文
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Changes in microrna (mir) profile and effects of mir-320 in insulin-resistant 3t3-l1 adipocytes
作者:
Ling, Hong-Yan;Ou, He-Sheng;Feng, Shui-Dong;Zhang, Xiao-Ying;Tuo, Qin-Hui;...
期刊:
Clinical and Experimental Pharmacology and Physiology ,2009年36(9):e32-e39 ISSN:0305-1870
通讯作者:
Liao, Duan-Fang
作者机构:
[Ling, Hong-Yan; Liao, Duan-Fang] Cent S Univ, Dept Pharmacol, Sch Pharmaceut Sci, Changsha, Hunan, Peoples R China.;[Tang, Cao-Ke; Zhang, Liang; Yin, Wei-Dong; Liao, Duan-Fang] Univ S China, Res Ctr Life Sci, Key Lab Atherosclerol Hunan Prov, Hengyang 421001, Hunan, Peoples R China.;[Chen, Lin-Xi; Gao, Zhi-Ping; Zhang, Xiao-Ying; Zhu, Bing-Yang; Ou, He-Sheng; Ling, Hong-Yan; Tuo, Qin-Hui; Liao, Duan-Fang] Univ S China, Res Ctr Life Sci, Key Lab Pharmacoprote Hunan Prov, Inst Pharm & Pharmacol, Hengyang 421001, Hunan, Peoples R China.;[Ling, Hong-Yan] Univ S China, Res Ctr Life Sci, Dept Physiol, Sch Med, Hengyang 421001, Hunan, Peoples R China.;[Feng, Shui-Dong] Univ S China, Res Ctr Life Sci, Sch Publ Hlth, Dept Epidemiol, Hengyang 421001, Hunan, Peoples R China.
通讯机构:
[Liao, Duan-Fang] U;Univ S China, Res Ctr Life Sci, Key Lab Atherosclerol Hunan Prov, 28 W Chengsheng Rd, Hengyang 421001, Hunan, Peoples R China.
关键词:
3T3-L1 adipocytes;Expression profile;Insulin resistance;MicroRNA;MiR-320;Phosphatidylinositol 3-kinase
摘要:
MicroRNAs (miRNAs) play essential roles in many biological processes. It is known that aberrant miRNA expression contributes to some pathological conditions. However, it is not known whether miRNAs play any role in the development of insulin resistance in adipocytes, a key pathophysiological link between obesity and diabetes. To investigate the function of miRNAs in the development of insulin resistance, using miRNA microarray analysis we compared miRNA expression profiles between normal insulin-sensitive 3T3-L1 adipocytes and 3T3-L1 adipocytes rendered insulin resistant following treatment with high glucose (25 mmol/L) and high insulin (1 μmol/L). Furthermore, adipocytes were transfected with specific antisense oligonucleotides against miRNA-320 (anti-miR-320 oligo) and the effects on the development of insulin resistance were evaluated. We identified 50 upregulated and 29 downregulated miRNAs in insulin-resistant (IR) adipocytes, including a 50-fold increase in miRNA-320 (miR-320) expression. Using bioinformatic techniques, the p85 subunit of phosphatidylinositol 3-kinase (PI3-K) was found to be a potential target of miR-320. In experiments with anti-miR-320 oligo, insulin sensitivity was increased in IR adipocytes, as evidenced by increases in p85 expression, phosphorylation of Akt and the protein expression of the glucose transporter GLUT-4, as well as insulin-stimulated glucose uptake. These beneficial effects of anti-miR-320 oligo were observed only in IR adipocytes and not in normal adipocytes. In conclusion, the miRNA profile changes in IR adipocytes compared with normal 3T3-L1 adipocytes. Anti-miR-320 oligo was found to regulate insulin resistance in adipocytes by improving insulin-PI3-K signalling pathways. The findings provide information regarding a potentially new therapeutic strategy to control insulin resistance. © 2009 Blackwell Publishing Asia Pty Ltd.
语种:
英文
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Advances in detection methods for EGFR gene mutation of lung cancer
作者:
奉水东(综述);谭红专(审校)
期刊:
中国肺癌杂志 ,2008年11(3):462-464 ISSN:1009-3419
通讯作者:
Tan, H.
作者机构:
[谭红专(审校)] Department of Epidemiology and Statistic, School of Public Health, Central South University, Changsha, Hunan 410008, China;Department of Epidemiology, School of Public Health, University of South China, Hengyang, Hunan 421001, China;[奉水东(综述)] Department of Epidemiology and Statistic, School of Public Health, Central South University, Changsha, Hunan 410008, China, Department of Epidemiology, School of Public Health, University of South China, Hengyang, Hunan 421001, China
通讯机构:
[Tan, H.] D;Department of Epidemiology and Statistic, School of Public Health, Central South University, China
关键词:
EGFR
摘要:
表皮生长因子受体(epidermal growth factor receptor,EGFR)基因突变与肺癌病人对抗肿瘤药物-吉非替尼的敏感性有关[1,2].而肺癌的前期临床试验[3,4]表明,在含铂类药物联合化疗失败后,吉非替尼仍有12%-18%的客观有效率,疾病控制率在50%左右,中位生存期约为7个月左右,受试者的症状改善且安全性良好,所以EGFR基因突变检测对于临床医生进行肺癌的个体化治疗具有重要的参考价值.关于EGFR基因突变检测的方法,文献报道主要来源于国外,但当前仍然没有一种完全适用于临床的方法.现就肺癌EGFR基因突变检测方法的研究作一综述.
语种:
英文
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PCR—SSCP检测非小细胞肺癌EGFR基因突变的敏感性分析
作者:
奉水东;谭红专;胡国斌;郭慧
期刊:
实用肿瘤杂志 ,2008年23(4):307-310 ISSN:1001-1692
作者机构:
[奉水东; 谭红专] 中南大学公共卫生学院流行病学与卫生统计学系;南华大学公共卫生学院流行病学教研室;[胡国斌; 郭慧] 湖南省肿瘤医院病理科
关键词:
肺肿瘤/遗传学;肺肿瘤/病理学;基因,erbB-1;聚合酶链反应;多态现象,单链构象;突变;受体,表皮生长因子/遗传学
摘要:
目的 探讨分析PCR—SSCP检测非小细胞肺癌EGFR基因突变的敏感性。方法 应用PCR—SSCP检测36例非小细胞肺癌标本的突变情况,阳性结果再进行DNA测序。结果通过PCR—SSCP分析,11例存在突变,突变率为30.69,0(11/36);PCR—SSCP分析阳性的结果通过DNA测序全为序列异常。结论 PCR—SSCP检测肺癌EGFR基因突变具有较高的敏感性。
语种:
中文
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PBL教学法在流行病学教学中的应用
作者:
李凤华;陈新;赵英;龙理良;奉水东
期刊:
实用医技杂志 ,2008年(17):2285-2286 ISSN:1671-5098
作者机构:
南华大学公共卫生学院;南华大学公共卫生学院 湖南衡阳421001
关键词:
PBL教学法;流行病学;应用
摘要:
目的:在流行病学教学中,引入新的教学模式-PBL教学法进行初步实践和探索。方法:在实施此教学方法之前,通过教师集体备课方式尽可能使设置的问题更合理,课程结束后对学生进行问卷调查以评估教学效果。结果:经改革的教学方法,收到学生和教师的良好反馈。结论:该教学方法能有效提高学生的学习动力和兴趣,有助于学生掌握扎实的基础知识,能增强学生分析和解决实际问题的能力。
语种:
中文
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社会支持与网络成瘾关系的队列研究
作者:
陈新;李凤华;龙理良;赵英;奉水东;...
期刊:
中国心理卫生杂志 ,2007年21(4):240-243 ISSN:1000-6729
作者机构:
[陈新; 李凤华; 龙理良; 赵英; 奉水东; 李勇] 湖南南华大学公共卫生学院流行病学教研室;湖南南华大学公共卫生学院流行病学教研室 衡阳421001
关键词:
网络成瘾;社会支持;队列研究;大学生
摘要:
目的:探讨社会支持与大学生网络成瘾的关系.方法:在某大学2000级医科类学生中以整群随机抽样的方法随机抽取10个小班382名学生为研究对象,分别以其进校第一学期末的社会支持与领悟社会支持得分的四分位数作为分界点,将研究对象分为社会支持低、中、高组,比较社会支持得分不同者毕业时网络成瘾检出率.结果:不论是按社会支持总分、领悟社会支持总分的高低分组,还是按两量表的各维度分的高低分组,社会支持得分低者网络成瘾的发生率均较社会支持评分高者高(领悟社会支持总分0~60、61~73、≥73者网络成瘾检出率分别为15.7%、7.9%、2.4%,χ2=9.49,P=0.009)网络成瘾检出率与社会支持总分及各维度得分均呈剂量反应关系.结论:较少的社会支持是大学生网络成瘾的危险因素.
语种:
中文
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Social Support and Posttraumatic Stress Disorder among Flood Victims in Hunan, China
作者:
Feng, Shuidong;Tan, Hongzhuan* ;Benjamin, Abuaku;Wen, Shiwu;Liu, Aizhong;...
期刊:
Annals of Epidemiology ,2007年17(10):827-833 ISSN:1047-2797
通讯作者:
Tan, Hongzhuan
作者机构:
Cent S Univ, Sch Publ Hlth, Changsha 410008, Hunan, Peoples R China.;Nanhua Univ, Dept Epidemiol, Sch Publ Hlth, Hengyang, Hunan, Peoples R China.;Univ Ottawa, OMNI Res Grp, Dept Obstet & Gynecol, Fac Med, Ottawa, ON, Canada.;Shanxi Med Univ, Dept Hlth Stat, Taiyuan, Shanxi, Peoples R China.;[Tan, Hongzhuan] Cent S Univ, Sch Publ Hlth, Xiangya Rd 110, Changsha 410008, Hunan, Peoples R China.
通讯机构:
[Tan, Hongzhuan] C;Cent S Univ, Sch Publ Hlth, Xiangya Rd 110, Changsha 410008, Hunan, Peoples R China.
关键词:
Epidemiology;Floods;Posttraumatic Stress Disorder;Social Support
摘要:
Purpose: To explore the relationship between social support and posttraumatic stress disorder (PTSD) among flood victims. Methods: A cross-sectional survey was carried out in 2000 among individuals who had suffered floods in 1998 in Hunan, China. Multistage sampling was used to select the subjects from the flood-affected areas. PTSD was diagnosed according to DSM-IV criteria, and social support was measured according to a social support rating scale. Data were collected through face-to-face interviews using a structured questionnaire. Multiple logistic regression analysis and confirmatory factor analysis was used to examine the relationship between social support and PTSD. Results: Out of a total of 25,478 subjects interviewed, 2336 (9.7%) were diagnosed as having PTSD. PTSD was significantly associated with total social support (odds ratio [OR] 0.80, 95% confidence interval [CI], 0.78-0.82), subjective support (OR 0.48, 95%CI, 0.44-0.52), and support utilization (OR 0.53, 95%CI, 0.49-0.57). Conclusion: PTSD in flood victims is significantly associated with social support; subjective support and support utilization may play more important roles in mitigating the impact of flood than objective support. © 2007 Elsevier Inc. All rights reserved.
语种:
英文
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网络成瘾大学生因特网使用的动机研究
作者:
陈新;李凤华;何爱桃;龙理良;赵英;...
期刊:
中华行为医学与脑科学杂志 ,2007年16(1):68-69 ISSN:1674-6554
作者机构:
[陈新; 李凤华; 何爱桃; 龙理良; 赵英; 奉水东; 李勇] 南华大学公共卫生学院流行病学教研室
关键词:
大学生;网络成瘾;因特网使用;动机
摘要:
目的 探讨网络成瘾大学生使用因特网的动机。方法 以整群随机抽样的方法随机抽取10个小班382名学生作为研究对象,以网络成瘾诊断量表、自编的因特网使用动机问卷对研究对象进行调查,比较网络成瘾者与未网络成瘾者使用因特网的动机。结果32名同学被诊断为网络成瘾,350名同学未网络成瘾。未网络成瘾大学生使用因特网的动机依次是交际上网、学习上网、从众上网、逃避现实上网、娱乐上网和交易上网,而网络成瘾大学生使用因特网的动机依次是逃避现实上网、从众上网、娱乐上网、交际上网、学习上网和交易上网。网络成瘾组逃避现实上网、从众上网、娱乐上网和交易上网的量化值[分别为(2.19±1.09)分,(1.99±1.07)分,(1.85±0.80)分,(0.78±1.11)分]较未网络成瘾组高,差异有非常显著性(P〈0.01)。网络成瘾大学生使用因特网的具体原因位于前五位的依次是“没头绪上网”、“同学拉我上网”、“玩网络游戏”、“心情不好上网”、“烦躁上网”。结论 网络成瘾大学生与未网络成瘾大学生使用因特网的动机不完全相同,逃避社会现实与从众是网络成瘾者使用因特网的主要动机。
语种:
中文
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社会支持对大学生上网动机影响的前瞻性研究
作者:
陈新;李凤华;何爱桃;赵英;龙理良;...
期刊:
中国健康心理学杂志 ,2007年15(3):216-218 ISSN:2096-4811
作者机构:
中国,南华大学公共卫生学院流行病学教研室(衡阳),421001;[奉水东; 陈新; 何爱桃; 赵英; 蔡练功; 龙理良; 李凤华] 南华大学
关键词:
大学生;社会支持;因特网;动机;前瞻性研究
摘要:
目的 探讨社会支持对大学生上网动机的影响.方法 以整群随机抽样的方法随机在某大学医科类学生中抽取10个小班383名学生作为研究对象,用社会支持量表调查其进校第一学期末的社会支持情况;用自编问卷调查研究对象大学四年级时因特网的使用动机,以社会支持总分的下四分位数作为分界点,将研究对象分为社会支持得分较低组与社会支持得分较高组,比较两组大学生的上网动机.结果 两组大学生在学习上网、交易上网、从众上网等方面差异无显著性(P>0.05),逃避现实上网、娱乐上网的频率社会支持得分较低组的大学生较社会支持得分较高组高(P<0.05);而交际上网得分社会支持得分较低组大学生较社会支持得分较高组低(P<0.05);社会支持得分较低的大学生在网上与陌生人聊天的频率与社会支持得分较高的大学生相同,但在网上与熟人交流的频率社会支持得分较低者较社会支持得分较高者低(P<0.05).结论 社会支持不同的人上网的动机存在差异.
语种:
中文
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在新的公共卫生形势下流行病学课程教学改革刍议
作者:
奉水东;陈新;赵英;龙理良;李凤华
期刊:
医学教育研究与实践 ,2006年14(6):726-727 ISSN:2096-3181
作者机构:
南华大学,公共卫生学院流行病学教研室,湖南,衡阳,421001
关键词:
流行病学;教学改革;课程体系设置
摘要:
流行病学是预防医学的主导学科,它的发展状况影响着整个预防医学的发展与水平.为了更好地适应当前和今后疾病防制形势的需要,本文对流行病学学科的定位与流行病学课程教学体系的科学设置进行了深入的剖析,并提出了可行的建议,力争能提高流行病学教学的时效性和实效性.
语种:
中文
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胰岛素抵抗大鼠血和血管壁一氧化氮变化分析
作者:
奉水东;凌宏艳;陈新;赵英;龙理良;...
期刊:
中国公共卫生 ,2006年22(7):797 ISSN:1001-0580
作者机构:
南华大学公共卫生学院流行病学教研室,湖南,衡阳,421001;中南大学公共卫生学院流行病学教研室;南华大学医学院生理学教研室;[凌宏艳; 奉水东; 陈新; 赵英; 李凤华; 龙理良] 南华大学
关键词:
胰岛素抵抗综合征;一氧化氮;SD大鼠;血管壁;高胰岛素血症;心血管疾病;Ⅱ型糖尿病;研究发现;血管病变;功能改变
摘要:
研究发现,胰岛素抵抗(insulin resistance,IR)及其所伴随的高胰岛素血症在Ⅱ型糖尿病心血管疾病的发展中起重要作用[1],可增加胰岛素抵抗综合征患者发生血管病变的危险[2].本文采用高果糖饲养SD大鼠建立IR模型,观察血和血管壁一氧化氮(NO)变化及其与血管内皮功能改变的相关性.结果报告如下.
语种:
中文
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生长抑制和DNA损伤基因表达对氮芥所致DNA损伤的监视作用
作者:
奉水东;陈锋;凌宏艳
期刊:
环境与健康杂志 ,2005年22(5):345-347 ISSN:1001-5914
作者机构:
[奉水东] 南华大学公共卫生学院流行病学教研室;[陈锋] 南华大学公共卫生学院院长办公室;[凌宏艳] 南华大学基础医学院生理学教研室
关键词:
DNA损伤;氮芥;烷化剂;Gadd基因
摘要:
目的探讨生长抑制和DNA损伤(growth arrest and DNA damage,Gadd)基因表达对DNA损伤的监视作用.方法以1、5 ng/μl的烷化剂氮芥处理人肝癌HepG2细胞3、24、48 h后提取细胞总RNA,运用逆转录(RT)-PCR方法检测Gadd基因的表达水平.结果分别用1 ng/μl氮芥处理肝癌细胞3、24、48 h和分别用1、5 ng/μl氮芥处理肝癌细胞24 h,Gadd基因的表达都呈现由增强(P<0.05)转为抑制的趋势,但两者均高于阴性对照水平(P>0.05).结论Gadd基因表达能够监视氮芥所致的DNA损伤.
语种:
中文
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