期刊论文

中文

实用肿瘤杂志

1001-1692

2008

23

4

307-310

10.3969/j.issn.1001-1692.2008.04.007

湖南省卫生厅资助项目（B200695）；

本校为其他机构

目的 探讨分析PCR—SSCP检测非小细胞肺癌EGFR基因突变的敏感性。方法 应用PCR—SSCP检测36例非小细胞肺癌标本的突变情况，阳性结果再进行DNA测序。结果通过PCR—SSCP分析，11例存在突变，突变率为30．69，0（11／36）；PCR—SSCP分析阳性的结果通过DNA测序全为序列异常。结论 PCR—SSCP检测肺癌EGFR基因突变具有较高的敏感性。

Objective To evaluate the sensitivity of PCR-SSCP in detection of EGFR gene mutations in non-small cell lung cancers. Methods EGFR mutations were detected by PCR-SSCP in 36 samples of non-small cell lung cancer,and then verified by DNA sequencing. Results In 11 out of 36 cases （30.6 %） EGFR mutations were detected by PCR-SSCP. All positive results were verified by DNA sequencing. Conclusion PCR-SSCP is a sensitive method to detec...