Objective To study the effect of Gadd genes expression in surveillance of DNA damage. Methods The human liver tumor cells were treated with alkylating agent（nitrogen mustard） and total RNA was extracted, the expression levels of Gadd genes were determined by RT-PCR. Results Treated with 1 ng/μl nitrogen mustard for 3 h, 24 h, 48 h respectively and with 1 ng/μl 5 ng/μl nitrogen mustard for 24 h respectively, the expression levels of Gadd genes increased at first （P〈0.05） and then showed a tendency of decrease, but both of them were highe...
