DNAzyme在金电极上自组装电化学生物传感器的制备
作者：
唐琼;袁亚莉;胡建邦;马丹丹;高阳阳
期刊：
南华大学学报(自然科学版) ,2013年27(2):63-66 ISSN：1673-0062
作者机构：
南华大学化学化工学院湖南衡阳;[唐琼] 湖南环境生物职业技术学院;[高阳阳; 胡建邦; 袁亚莉; 马丹丹] 南华大学
关键词：
自组装;二茂铁(Fc);循环伏安法
摘要：
利用巯基与金表面Au-S键的自组装技术,可以将巯基修饰的DNAzyme固定在金电极表面形成电化学生物传感器,并以含有[Fe（CN）6]3-/4-的磷酸缓冲液（PBS）作为电化学检测底液,利用循环伏安法初步研究该修饰电极的电化学行为和修饰过程的电化学表征.DNAzymes自组装膜的存在明显抑制了二茂铁与[Fe（CN）6]3-/4-之间的电子转移.结果表明,基于巯基修饰的DNAzyme在金电极表面能够发生自组装作用,在金电极表面形成较为稳固的自组装DNAzyme膜.
语种：
中文
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立足学科特色 改革和创新化学实验教学
作者：
袁亚莉;周昕;刘慧君;聂长明
期刊：
实验室研究与探索 ,2013年32(8):161-163,173 ISSN：1006-7167
作者机构：
南华大学化学化工学院,湖南衡阳,421001;[聂长明; 刘慧君; 袁亚莉; 周昕] 南华大学
关键词：
实验教学;教学体系;开放性实验;教学管理
摘要：
化学实验教学改革一直是高校化学教学工作者关注的重点和热门话题。南华大学化学实验中心以建设省级示范实验室为动力，从改革实验中心运行机制入手，建立相对独立的《大学化学实验》课程体系；创新化学实验教学，大力推动开放性化学实验教学；在建设高水平的实验教师队伍、优化实验教学管理等方面均进行了一系列有益的探索，取得了良好的效果。使实验中心成为能独立、有效地进行实验教学和科学研究的基地。
语种：
中文
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DNAzyme based electrochemical sensors for trace uranium
作者：
Tang, Qiong;Yuan, Yali* ;Xiao, Xilin;Guo, Ping;Hu, Jianbang;...
期刊：
Microchimica Acta ,2013年180(11-12):1059-1064 ISSN：0026-3672
通讯作者：
Yuan, Yali
作者机构：
[Gao, Yangyang; Yuan, Yali; Hu, Jianbang; Tang, Qiong; Xiao, Xilin; Ma, Dandan] Univ South China, Sch Chem & Chem Engn, Hengyang 421001, Hunan, Peoples R China.;[Guo, Ping] Univ South China, Sch Math & Phys, Hengyang 421001, Hunan, Peoples R China.
通讯机构：
[Yuan, Yali] U;Univ South China, Sch Chem & Chem Engn, Hengyang 421001, Hunan, Peoples R China.
关键词：
DNAzyme;Electrochemical;Ferrocene;UO 2 2+
摘要：
We have developed a uranyl-specific DNAzyme that was immobilized on the surface of a gold electrode to give a highly sensitive and selective biosensor for uranyl ion. The typical DNAzyme system consisted of the RNA (rA) as the substrate (ADNA), and the other strand is the enzyme (TDNA) with a ferrocene (Fc). The presence of uranyl ion induces the cleavage of the DNA substrate strand at the rA position to form two fragments. The Fc unit thereby is released from the surface of the electrode, and this results in a decreased peak current. This electrochemical biosensor has a dynamic range from 2 nM to 14 nM of uranyl ion, with a detection limit at 1 nM. It exhibits high sensitivity and excellent selectivity over other metal ions, and thus represents a promising technique for simple, fast, on-site, and real-time electrochemical sensing of UO2(II) ion. It also serves as a guide in choosing different methods for designing electrochemical sensors for other metal ions. Figure We have developed a uranyl-specific DNAzyme that was immobilized on the surface of a gold electrode to give a highly sensitive and selective biosensor for uranyl ion. The typical DNAzyme system consisted of the RNA as the substrate and the other strand is the enzyme with a ferrocene (Fc). This electrochemical biosensor exhibits high sensitivity and excellent selectivity, and represents a promising technique for simple, fast, on-site, and real-time electrochemical sensing of UO2(II) ion.
语种：
英文
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氨基化改性Fe_3O_4/SiO_2复合磁性材料的制备以及对铀(VI)的吸附研究
作者：
胡建邦;袁亚莉;唐琼;黄芬;陈杭;...
期刊：
应用化工 ,2012年41(12):2067-2070,2074 ISSN：1671-3206
作者机构：
[胡建邦; 袁亚莉; 唐琼; 黄芬; 陈杭; 马丹丹; 高阳阳; 李乐; 梁俊] 南华大学化学化工学院
关键词：
铀(VI);吸附;氨基化
摘要：
利用化学共沉淀法制备四氧化三铁磁性纳米粒子,依次用正硅酸乙酯(TEOS)和3-氨丙基三乙氧基硅烷(APTES)进行表面修饰,制备了表面氨基化改性的Fe_3O_4/SiO_2复合粒子;采用红外(IR)、扫描电镜(TEM)、振动样品磁强计(VSM)、热综合分析仪(TGA)、X射线粉末衍射分析(XRD)对其结构进行表征。结果表明,氨基化改性Fe_3O_4/SiO_2复合磁性粒子的粒径在500 nm,饱和磁化强度为26.54 emu/g。对水溶液中铀(VI)的最佳吸附条件为:吸附温度35 ℃,吸附时间30 min, pH为4.5,复合材料用量为0.1 g,在此条件下,对铀酰离子的平衡吸附率为98%。方法简单,吸附后的复合磁性材料可方便地进行富集和回收。
语种：
中文
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18-冠-6/LA/Fe_3O_4复合磁性纳米粒子的制备及其吸附性能研究
作者：
黄芬;袁亚莉;梁俊;王雄斌;聂超;...
期刊：
原子能科学技术 ,2012年46(7):786-790 ISSN：1000-6931
通讯作者：
Yuan, Y.-L.(yuanyali6439@126.com)
作者机构：
[袁亚莉; 聂超; 王雄斌; 韩玉波; 梁俊; 胡建邦; 唐琼; 黄芬] School of Chemistry and Chemical Engineering, University of South China, Hengyang 421001, China
通讯机构：
School of Chemistry and Chemical Engineering, University of South China, China
关键词：
冠醚;复合磁纳米粒子;吸附铀酰离子
摘要：
Fe3O4磁性纳米粒子（MNPs）比表面积大、吸附能力强,且在外磁场作用下易于液固分离,冠醚具有对铀酰离子选择性配位的能力。本研究制备了兼具两者特点和优势的18-冠-6/LA/Fe3O4复合磁性纳米粒子。其结构经红外（IR）、扫描电镜-X射线能谱（SEX/EDX）、振动样品磁强计（VSM）、热综合分析仪（TGA）进行表征,表明18-冠-6/LA/Fe3O4复合磁性纳米粒子的粒径为12～23nm,饱和磁化强度为56.34emu/g。用制得的复合磁性纳米粒子对溶液中铀酰离子进行初步吸附实验,在选定的条件下,平衡吸附率达95%,操作方法快速简便,吸附后的磁粒子可方便地进行富集和回收。
语种：
中文
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A novel mesoporous material for uranium extraction, dihydroimidazole functionalized SBA-15
作者：
Yuan, Li-Yong;Liu, Ya-Lan;Shi, Wei-Qun* ;Li, Zi-jie;Lan, Jian-Hui;...
期刊：
JOURNAL OF MATERIALS CHEMISTRY B ,2012年22(33):17019-17026 ISSN：2050-750X
通讯作者：
Shi, Wei-Qun
作者机构：
[Yuan, Li-Yong; Li, Zi-jie; Chai, Zhi-Fang; Shi, Wei-Qun; Lan, Jian-Hui; Feng, Yi-Xiao] Chinese Acad Sci, Inst High Energy Phys, Key Lab Nucl Analyt Tech, Beijing 100049, Peoples R China.;[Liu, Ya-Lan; Yuan, Ya-Li] Univ S China, Sch Chem & Chem Engn, Dept Analyt Chem, Hengyang 421001, Peoples R China.;[Zhao, Yu-Liang] Chinese Acad Sci, Inst High Energy Phys, Key Lab Biomed Effects Nanomat & Nanosafety, Beijing 100049, Peoples R China.
通讯机构：
[Shi, Wei-Qun] C;Chinese Acad Sci, Inst High Energy Phys, Key Lab Nucl Analyt Tech, Beijing 100049, Peoples R China.
摘要：
Due to the rapid development of the nuclear power industry, and consequently, the nuclear accident in Fukushima, much attention has been paid to novel materials for the efficient and rapid separation, removal and recovery of nuclear fuel associated radionuclides from aqueous solutions. Herein, a novel mesoporous material, dihydroimidazole functionalized SBA-15 (DIMS), was synthesized via a post-grafting method and used as an efficient sorbent for the extraction of U(VI) from aqueous solution. The synthesized material was found to possess highly ordered mesoporous structures with a large surface area and a uniform pore diameter. The sorption tests under various conditions demonstrated that the sorption of U(VI) by DIMS was fast, with an equilibrium time of less than 10 min. Additionally, the maximum sorption capacity reached 268 mg g−1 at pH 5.0 ± 0.1. Changes in the solid-to-liquid ratio (msorbent/Vsolution) did not have any remarkable effect on the U(VI) sorption. Besides, the sorbed U(VI) can be easily desorbed by 0.01 mol L−1 or more concentrated HNO3 solution, resulting in a U(VI) solution with a concentration factor of 300 at a solid–liquid ratio as low as 0.013 g L−1. The reclaimed sorbent can be reused with no obvious decrease in the sorption capacity. The selectivity of the DIMS sorbent for U(VI) ions was found to be fairly desirable by the sorption tests with the solutions containing a range of competing metal ions.
语种：
英文
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立足本校学科特色,创新《医学化学》课堂兴趣教学
作者：
廖力夫;肖锡林;聂长明;袁亚莉;邓健
期刊：
检验医学教育 ,2012年(01):26-28
作者机构：
南华大学化学化工学院
关键词：
医学化学;课堂教学;兴趣;创新
摘要：
兴趣是最好的老师,是学习的直接动力,决定着学生学习活动的方向和效率。该文以南华大学《医学化学》课程组多年课堂教学实践为基础,结合化学学科的特点,重点讨论了如何在课堂教学中,培养和激发学生学习医学化学的兴趣,努力为培养医学生的动手能力和创新能力打下良好的基础。
语种：
中文
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A high efficient sorption of U(VI) from aqueous solution using amino-functionalized SBA-15
作者：
Liu, Yalan;Yuan, Liyong;Yuan, Yali* ;Lan, Jianhui;Li, Zijie;...
期刊：
Journal of Radioanalytical and Nuclear Chemistry ,2012年292(2):803-810 ISSN：0236-5731
通讯作者：
Yuan, Yali
作者机构：
[Yuan, Yali; Liu, Yalan] Univ S China, Sch Chem & Chem Engn, Dept Analyt Chem, Hengyang 421001, Peoples R China.;[Lan, Jianhui; Feng, Yixiao; Yuan, Liyong; Chai, Zhifang; Li, Zijie; Shi, Weiqun] Chinese Acad Sci, Inst High Energy Phys, Key Lab Nucl Analyt Tech, Beijing 100049, Peoples R China.;[Yuan, Yali; Lan, Jianhui; Feng, Yixiao; Yuan, Liyong; Li, Zijie; Shi, Weiqun; Zhao, Yuliang] Chinese Acad Sci, Key Lab Biomed Effects Nanomat & Nanosafety, Inst High Energy Phys, Beijing 100049, Peoples R China.
通讯机构：
[Yuan, Yali] U;Univ S China, Sch Chem & Chem Engn, Dept Analyt Chem, Hengyang 421001, Peoples R China.
关键词：
Uranium;Sorption;Mesoporous silica;Functionalization
摘要：
Uranium is one of the most hazardous heavy metal due to its long half-life radioactivity, high toxicity and mobility as aqueous uranyl ion (UO 2+ 2 ) under ordinary environmental conditions. Herein, amino functionalized SBA-15 (APSS) was developed as a rapid and efficient sorbent for removal of U(VI) from the environment. The APSS sample was synthesized by grafting method and was characterized by SEM, NMR, SAXS, and N 2 sorption/desorption isothermal experiments. The sorption of U(VI) by APSS was investigated under different conditions of pH, contact time, initial U(VI) concentration, ionic strength and solid-liquid ratio. The results show that the sorption of U(VI) by APSS is strongly dependent on pH but independent of ionic strength and solid-liquid ratios (m/V). The sorption is ultrafast with an equilibrium time of less than 30 min, and the sorption capacity is as large as 409 mg/g at pH 5.3 ± 0.1. Besides, the U(VI) sorption by APSS from extremely diluted solution and the desorption of U(VI) from APSS were also studied. It is found that 100 mg of APSS can almost completely remove the U(VI) ions from 4 L aqueous solution with the U(VI) concentration as low as 4.2 ppb and the sorbed U(VI) can be completely desorbed by 0.1 mol/L nitric acid. The results strongly reveal the high performance of the APSS material in the removal and preconcentration of U(VI) from the aqueous solution. © 2012 Akadémiai Kiadó, Budapest, Hungary.
语种：
英文
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A square wave voltammetric method for the detection of microorganism populations using a MWNT-modified glassy carbon electrode
作者：
Xiao, Xilin* ;Zhu, Guizhi;Liao, Lifu;Liu, Bo;Yuan, Yali;...
期刊：
Electrochimica Acta ,2012年74:105-110 ISSN：0013-4686
通讯作者：
Xiao, Xilin
作者机构：
[Wu, Yimou; Yuan, Yali; Wang, Yongsheng; He, Jun; Xiao, Xilin; Liao, Lifu; He, Bo] Univ S China, Coll Chem & Chem Engn, Hengyang 421001, Hunan, Peoples R China.;[Wu, Yimou; Yuan, Yali; Wang, Yongsheng; He, Jun; Xiao, Xilin; Liao, Lifu; He, Bo] Univ S China, Coll Publ Hlth, Pathogen Biol Inst, Hengyang 421001, Hunan, Peoples R China.;[Zhu, Guizhi; Liu, Bo; Xiao, Xilin] Univ Florida, Ctr Res Bio Nano Interface, Dept Chem & Physiol, Gainesville, FL 32611 USA.;[Zhu, Guizhi; Liu, Bo; Xiao, Xilin] Univ Florida, Dept Funct Genom, Strands Canc Ctr, UF Genet Inst, Gainesville, FL 32611 USA.;[Zhu, Guizhi; Liu, Bo; Xiao, Xilin] Univ Florida, McKnight Brain Inst, Gainesville, FL 32611 USA.
通讯机构：
[Xiao, Xilin] U;Univ S China, Coll Chem & Chem Engn, Hengyang 421001, Hunan, Peoples R China.
关键词：
Square wave voltammetry (SWV);Cyclic voltammetry (CV);Multiwalled carbon nanotube (MWNT);Glassy carbon electrode;Escherichia coli O157:H7
摘要：
A novel method for determination of trace amounts of microorganism populations in solution was developed by using a multiwalled carbon nanotube (MWNT) modified glassy carbon electrode and square wave voltammetry (SWV). The simultaneous combination of MWNT and SWV allowed the electrochemical signal of electro-active materials in Escherichia coli O157:H7 (E. coli) to be dramatically amplified. Compared with a bare glassy carbon electrode, the MWNT-modified glassy carbon electrode showed catalytic properties in the oxidation of electro-active materials on cell surfaces. Moreover, SWV was proved to be more sensitive than cyclic voltammetry (CV) for investigation of the electrochemical behavior of cells. In this paper, a linear relationship was obtained between the SWV peak current and the cell concentration in the range 2 × 102–2 × 108 cell mL−1 with a detection limit of 2 × 102 cell mL−1. The effect of antibiotic drug Gentamycin Sulfate injection (GSI) on the growth of E. coli was also investigated.
语种：
英文
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AE-HG-AFS测定长期汞暴露人群补硒后尿中硒的形态
作者：
黄笑寒;李玉锋;林婧;李柏;瞿丽雅;...
期刊：
分析试验室 ,2012年31(1):11-15 ISSN：1000-0720
作者机构：
[黄笑寒; 李玉锋; 林婧; 李柏; 高愈希; 陈春英] 中国科学院高能物理研究所多学科研究中心;[袁亚莉] 南华大学化学化工学院;福州大学食品安全分析与检测教育部重点实验室;[瞿丽雅; 董泽琴] 贵州环境科学研究设计院;国家纳米科学中心中国科学院纳米生物效应与安全性重点实验室
关键词：
阴离子交换色谱;氢化物发生原子荧光光谱;硒;形态;尿样
摘要：
建立了一种利用阴离子交换高效液相色谱与氢化物发生原子荧光光谱联用同时测定四、六价硒及硒代半胱氨酸（SeCys）形态的方法。优化了六价硒的还原条件及仪器检测参数,以不同浓度的柠檬酸铵作为流动相,在10 min内同时分离了四、六价硒及硒代半胱氨酸（SeCys）。采用加标法定量,加标回收率在90%～119%之间,相对标准偏差为1.6%～3.1%（100μg/L）,四、六价硒及硒代半胱氨酸（SeCys）的检出限分别为0.32μg/L、0.47μg/L和0.44μg/L（进样量为100μL）。应用该法对长期汞暴露人群补硒后尿中的小分子硒的形态进行了分析,仅检测到硒代半胱氨酸（SeCys）。
语种：
中文
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Electrochemical sensor based on a poly(para-aminobenzoic acid) film modified glassy carbon electrode for the determination of melamine in milk
作者：
Liu, Ya Ting;Deng, Jian* ;Xiao, Xi Lin;Ding, Li;Yuan, Ya Li;...
期刊：
Electrochimica Acta ,2011年56(12):4595-4602 ISSN：0013-4686
通讯作者：
Deng, Jian
作者机构：
[Wang, Li Li; Li, Xiu Ting; Liu, Ya Ting; Yan, Xiao Na; Li, Hui; Deng, Jian; Yuan, Ya Li; Xiao, Xi Lin] Univ S China, Sch Chem & Chem Engn, Hengyang 421001, Hunan, Peoples R China.;[Ding, Li] Hunan Prov Ctr Dis Prevent & Control, Changsha 410005, Hunan, Peoples R China.;[Deng, Jian] Univ S China, Sch Chem & Chem Engn, 28 Changsheng W Rd, Hengyang 421001, Hunan, Peoples R China.
通讯机构：
[Deng, Jian] U;Univ S China, Sch Chem & Chem Engn, 28 Changsheng W Rd, Hengyang 421001, Hunan, Peoples R China.
关键词：
Molecularly imprinted polymer;Poly(para-aminobenzoic acid);Electropolymerization;Melamine;Milk
摘要：
A novel and simple sensor is developed in this paper for melamine detection, which is based on an electropolymerized molecularly imprinted polymer (MIP) of para-aminobenzoic acid (pABA). The poly(para-aminobenzoic acid) (P-pABA) film was deposited in a pABA solution by potentiodynamic cycling of potential with and without the template (melamine) on a glassy carbon electrode. The surface feature of the modified electrode was characterized by electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV). The molecular imprinted sensor was tested by differential pulse voltammetry (DPV) to verify the changes in redox peak currents of hexacyanoferrate. Several important parameters controlling the performance of the P-pABA were investigated and optimized. In the optimal conditions, the relative redox peak currents of hexacyanoferrate were linear. The concentration of melamine ranged from 4.0 μM to 0.45 mM, with a linear correlation coefficient of 0.9992. The detection limit was 0.36 μM (S/N = 3). The MIP sensor was successfully applied to the determination of melamine in milk products and showed high selectivity, sensitivity, and reproducibility. The results of this research demonstrate that it is feasible to use the molecular imprinting methodology when preparing sensing devices for analytes that are electrochemically inactive. © 2011 Elsevier Ltd. All rights reserved.
语种：
英文
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Carbon paste electrode modified with a binuclear manganese complex as a sensitive voltammetric sensor for tryptophan
作者：
Xu, Jinsheng* ;Yuan, Yali;Li, Wei;Deng, Peihong;Deng, Jian
期刊：
Microchimica Acta ,2011年174(3-4):239-245 ISSN：0026-3672
通讯作者：
Xu, Jinsheng
作者机构：
[Deng, Peihong; Xu, Jinsheng; Li, Wei] Hengyang Normal Univ, Dept Chem & Mat Sci, Hengyang 421008, Peoples R China.;[Yuan, Yali; Deng, Jian] Nanhua Univ, Coll Chem & Chem Engn, Hengyang 421001, Peoples R China.
通讯机构：
[Xu, Jinsheng] H;Hengyang Normal Univ, Dept Chem & Mat Sci, Hengyang 421008, Peoples R China.
关键词：
Binuclear manganese(II) complex;Carbon paste electrode;Tryptophan
摘要：
We report on a carbon paste electrode that was modified with a binuclear manganese(II) complex by the drop-coating method. A study on the mechanism of the electro-oxidation of tryptophan (Trp) at this electrode indicated that it enables Trp to be determined with good sensitivity and selectivity. Second-order derivative linear sweep voltammetry at pH 4. 1 revealed that a sensitive anodic peak appears at 812 mV (vs. SCE) whose current is proportional to the concentration of Trp in the concentration range from 0. 1 to 1. 0 μmol L -1 and 1. 0 to 80 μmol L -1 , with a detection limit (S/N = 3) of 0. 08 μmol L -1 (60 s of accumulation). The method was applied to the determination of Trp in amino acid injection solutions with satisfactory results. © 2011 Springer-Verlag.
语种：
英文
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RP-HG-AFS联用研究长期汞暴露人群补硒前后血清中汞的形态
作者：
黄笑寒;李玉锋;董泽琴;李柏;高愈希;...
期刊：
分析试验室 ,2011年30(7):14-17 ISSN：1000-0720
作者机构：
[黄笑寒; 袁亚莉; 陈春英] 南华大学化学化工学院;[李玉锋; 李柏; 高愈希] 中国科学院高能物理研究所多学科研究中心;[董泽琴; 瞿丽雅] 贵州省环境科学研究设计院;国家纳米科学中心
关键词：
反相色谱;氢化物发生原子荧光光谱;无机汞;甲基汞
摘要：
建立了反相色谱-氢化物发生原子荧光光谱联用测定血清中汞形态(无机汞和甲基汞)的方法,优化了载流液,剂以及2-巯基乙醇的浓度,以正辛醇作为消泡剂,成功地解决了测定血清样品时原子荧光仪器内气液分离器中产生大量泡沫的难题。采用反相色谱柱对无机汞和甲基汞形态进行了分离,回收率在95%～110%之间,无机汞和甲基汞的精密度RSD(n=7)分别为3.3%和4.0%,二者的检出限分别为0.28μg/L和0.11μg/L。当无机汞和甲基汞形态浓度范围为2～30μg/L时,两种形态均可得到良好的线性关系,线性相关系数均>0.9990。通过测定标准物质人发(GBW 07601)和鱼样(IAEA MA-B-3/TM)评价方法的可靠性。方法用于分析长期汞暴露地区人群口服补硒剂后血清中汞的化学形态并定量,结果发现硒能促进人体内汞的排出。
语种：
中文
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18-冠-6/Fe3O4复合磁性纳米粒子的制备及性能研究
作者：
黄芬;袁亚莉;梁俊;王雄斌;聂超;...
作者机构：
[黄芬; 袁亚莉; 梁俊; 王雄斌; 聂超; 韩玉波; 胡建邦; 唐琼] 南华大学化学化工学院
会议名称：
中国核学会2011年年会
会议时间：
2011-10-11
会议地点：
贵阳
会议论文集名称：
中国核学会2011年年会论文集
关键词：
冠醚;复合磁纳米粒子;油酸
摘要：
Fe3O4磁性纳米粒子（MNPs）表面积大、吸附率高、且在外磁场作用下易于磁液分离,冠醚具有对铀、钍等离子选择性配位的能力。为结合两者的特点和优势,本研究制备了高吸附性能和易于磁液分离的18-冠-6/LA/Fe3O4复合磁纳米粒子。其结构经IR、SEM、VSM、DGA等进行表征,表明18-冠-6/Fe3O4复合纳米粒子的粒径在12-23 nm,饱和磁化强度为56.338 emu/g,具有超顺磁性和较强的抗氧化性能。用制得的复合磁性纳米粒子对溶液中铀、钍等离子进行吸附实验,具有吸附量大、快速高效、易于磁液分离等优点,可方便进行富集和定位,利于回收和重复利用。
语种：
中文
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Fe3O4磁流体对铀（VI）的吸附研究
作者：
贾继云;袁亚莉;黄芬;徐丽华;刘雅兰;...
期刊：
南华大学学报(自然科学版) ,2011年25(2):66-69 ISSN：1673-0062
作者机构：
南华大学化学化工学院,湖南衡阳,421001;[袁亚莉; 刘雅兰; 徐丽华; 黄笑寒; 黄芬; 贾继云] 南华大学
关键词：
纳米粒子;四氧化三铁;铀(VI);动力学模型
摘要：
为降低铀对环境的影响，研究纳米磁流体对铀（VI）的吸附行为．用沉淀法制备了磁粒径10～20nm的Fe2O2磁流体，考察了该磁磁流体对铀（VI）吸附的各种影响因素，结果表明：在纳米磁流体浓度为2．5∥L、温度35~C、pH=3、吸附时间t=30min的条件下，对3×1014mol／L铀的吸附萃取率可达到84．15％．该吸附过程符合假设的二级动力学模型．
语种：
中文
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TiO2 Nanoparticles Co-Doped with Silver and Nitrogen for Antibacterial Application
作者：
Yuan, Yali* ;Ding, Jianqiang;Xu, Jinsheng;Deng, Jian;Guo, Jianbo
期刊：
JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY ,2010年10(8):4868-4874 ISSN：1533-4880
通讯作者：
Yuan, Yali
作者机构：
[Yuan, Yali; Deng, Jian] Univ S China, Sch Chem & Chem Engn, Hengyang 421001, Peoples R China.;[Ding, Jianqiang] Zhenhai Ctr Dis Control & Prevent, Ningbo 315200, Peoples R China.;[Xu, Jinsheng] Hengyang Normal Univ, Dept Chem & Mat Sci, Hengyang 421008, Peoples R China.;[Guo, Jianbo] Univ S China, Sch Publ Hlth, Hengyang 421001, Peoples R China.
通讯机构：
[Yuan, Yali] U;Univ S China, Sch Chem & Chem Engn, Hengyang 421001, Peoples R China.
关键词：
Antibacterial property;Co-doped;Nanoparticle;TiO 2
摘要：
We have prepared a series of TiO<inf>2</inf> nanoparticles for antibacterial applications. These TiO<inf>2</inf> nanoparticles were prepared by the hydrolysis precipitation method with Ti(OBu)<inf>4</inf>, silver nitrate and ammonia. Crystal structure, particle size, interfacial structure and UV-visible light response of the prepared nanoparticles were characterized by X-ray diffraction measurements (XRD), Transmission electron microscopy (TEM), Fourier-transform infrared spectroscopy (FTIR) and UV-Vis diffuse reflectance spectroscopy (UV-Vis-DRs). The XRD spectra showed that all samples were anatase structure calcined at 450 °C for 3 hours. The Ag doping made the peak of diffraction wider. The results of TEM showed that the nanoparticles of TiO <inf>2</inf>, N-TiO<inf>2</inf> and 1% Ag-N-TiO<inf>2</inf> were all spherical in shape and well distributed with a mean size of 19.8 nm, 39.2 nm and 20.7 nm, respectively. N doping caused the nanoparticle size to increase, while, when the doped amount of Ag<sup>+</sup> increased, the TiO<inf>2</inf> particle size decreased. The FTIR revealed that Ag and N doping of TiO<inf>2</inf> appeared to have strong absorption by -OH group and showed the characteristic absorption band of NH<sup>+</sup><inf>4</inf> and Ag. The UV-Vis-DRs indicated that the absorption band of Ag-N co-doped TiO<inf>2</inf> had red shift and that the optical absorption response (between 400 nm and 700 nm) had obvious enhancement. The antibacterial properties of nanoparticles were investigated by agar diffusion method toward Escherichia coli and Bacillus subtilis. The results indicated that both Ag-and N-doped TiO<inf>2</inf> could increase the antibacterial properties of TiO<inf>2</inf> nanoparticles under fluorescent light irradiation. A 1% Ag-N-TiO<inf>2</inf> had the highest antibacterial activity with a clear antibacterial circle of 33.0 mm toward Escherichia coli and 22.8 mm toward Bacillus subtilis after cultivation for 24 hours. Copyright ©2010 American Scientific Publishers.
语种：
英文
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双环[2.2.1]-2-庚烯-5, 6-二甲酸镍配合物的合成、晶体结构及热稳定性
作者：
Xu Jin-Sheng* ;Yuan Ya-Li
期刊：
无机化学学报 ,2010年26(5):917-920 ISSN：1001-4861
通讯作者：
Xu Jin-Sheng
作者机构：
[Xu Jin-Sheng] Hengyang Normal Univ, Dept Chem & Mat Sci, Hengyang 421008, Hunan, Peoples R China.;[Yuan Ya-Li] Nanhua Univ, Coll Chem & Chem Engn, Hengyang 421001, Hunan, Peoples R China.
通讯机构：
[Xu Jin-Sheng] H;Hengyang Normal Univ, Dept Chem & Mat Sci, Hengyang 421008, Hunan, Peoples R China.
关键词：
镍(Ⅱ)配合物;晶体结构;热重分析
摘要：
镍是生物体中必须的痕量元素,常成为一些酶的活性中心,因而模拟镍酶并研究其结构,一直是生物无机化学等领域的重要研究课题[1-2],一近年来镍配合物在分子磁体,超氧歧化物,生物活性和催化等方面的研究已有比较多的报道[3-4],但主要是刚性芳香羧酸,而对于柔性环状羧酸配体体系研究不多,且文献报道较少[5-8].
语种：
中文
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医科类基础化学实验教学改革探讨
作者：
廖力夫;匡汀;肖锡林;袁亚莉;周昕;...
期刊：
山西医科大学学报 ,2010年12(5):501-504 ISSN：1007-6611
作者机构：
南华大学化学化工学院,衡阳,421001;[何博; 袁亚莉; 匡汀; 肖锡林; 肖静水; 廖力夫; 王晓娟; 周昕] 南华大学
关键词：
基础化学;教学方法;教学内容;成绩考核
摘要：
医科类基础化学是医学专业的重要基础课程。如何优化医科类基础化学实验教学,培养高素质的医学专业人才是医药院校化学教师努力追求的目标。为了激发学生的学习兴趣,提高教学质量,对医科类基础化学实验课程的教学内容、教学方法和成绩考核等方面进行了改革,并对结果进行了讨论分析。
语种：
中文
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新型制药工程专业人才培养的实践教学体系研究
作者：
谭倪;聂长明;袁亚莉;刘慧君;肖新荣;...
期刊：
化工高等教育 ,2010年27(4):63-66 ISSN：1000-6168
作者机构：
南华大学,化学化工学院,湖南,衡阳,421001;湘南学院,化学与生命科学系,湖南,郴州,423000;[谭倪; 聂长明; 刘慧君; 袁亚莉; 肖新荣; 何军] 南华大学;[陶李明] 湘南学院
关键词：
实践教学体系;制药工程;新型人才
摘要：
制药工程是一个多学科交叉、实践性很强的新兴专业。为培养符合时代要求的创新型、创业型和复合型的制药工程专业人才,作者提出了实践教学不仅应紧握产学研合作教育这一高等教育的指挥棒,而且还要紧扣综合性实验、开放式实验、毕业实习和以团队为单位开展毕业设计（论文）这四种基本形式。
语种：
中文
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“研究性学习”教育教学模式的探索与实践
作者：
聂长明;文松年;袁亚莉;邓健;廖力夫
期刊：
中国地质教育 ,2009年18(02):66-70 ISSN：1006-9372
作者机构：
南华大学,化学化工学院,湖南,衡阳,421001;[文松年; 聂长明; 袁亚莉; 邓健; 廖力夫] 南华大学
关键词：
研究性学习;教学模式;实践模式
摘要：
\"研究性学习\"教育是指在教师指导下,研究生从自然、社会和生活实际中选择和确定相关的专题进行研究,并在研究过程中主动获取知识、应用知识、解决问题的学习活动。其目标是:获得亲身参与研究的体验,培养发现问题和解决问题的能力,培养收集、分析和利用信息的能力,学会分享与合作,培养科学态度和科学道德,培养对社会的责任心和使命感。本文结合\"研究性学习\"的教学实践,从\"研究性学习\"教育的必要性、实践模式以及实践方法3个方面阐述了\"研究性学习\"教育教学模式。
语种：
中文
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