This paper reports the electrochemical biosensor using DNAzyme with thiolated- modified through Au-S self-assembly technology, which is bound on the surface of the gold electrode, and utilize phosphate buffer solution (PBS) with [ Fe(CN) 6 ] 3 -/4- as the solu- tion for the electrochemical detection. Then, we study the electrochemical characterization and modification process of the modified electrode by cyclic vohammetry, the electrochemi- cal transfer significantly decreased between [ Fe(CN) 6 ] 3-/4- and Fc on the gold electrode modifi...