Development of Ti-Al intermetallic compounds porous material with reactively synthesizing and its biological application
作者：
Xiao, Xilin* ;Liao, Lifu;Nie, Changming;Zhou, Xin;He, Bo;...
作者机构：
[Deng, Chang'ai; Xiao, Xilin; Liao, Lifu; Zhou, Xin; Nie, Changming; He, Bo] Univ South China, Coll Chem & Chem Engn, Hengyang 421001, Peoples R China.
会议名称：
International Conference on Materials, Environmental and Biological Engineering
会议时间：
2015-03-28
会议地点：
Guilin
会议主办单位：
[Xiao, Xilin;Liao, Lifu;Nie, Changming;Zhou, Xin;He, Bo;Deng, Chang'ai] Univ South China, Coll Chem & Chem Engn, Hengyang 421001, Peoples R China.
会议论文集名称：
International Conference on Materials, Environmental and Biological Engineering, Part 1: Guilin(CN).28-30 March 2015
关键词：
Staphylococcus aureus ssp. anaerobius (S. aureus);Penicillin G;Targeted drug delivery;Bone;Ti-Al intermetallic compounds porous material
摘要：
Bacterial infection is one of the most common problems after orthopedic implant surgery. If not prevented, bacterial infection can result in serious and life threatening conditions such as osteomyelitis. Thus, in order to reduce chances of such serious complication, patients are often subjected to antibiotic drug therapy for 6-8 weeks after initial surgery. The antibiotics are systemically delivered either intravenously, intramuscularly or topically. Systemic antibiotic delivery entails certain drawbacks such as systemic toxicity and limited bioavailability. Further, in order for the drug to be effective at the site of implantation, high doses are required, which can result in undesired side effects in patients. Thus, local antibiotic therapy is the preferred way of administering drugs. To that end, we have developed Ti-Al intermetallic compounds porous material for local delivery of antibiotics off-implant at the site of implantation. The Ti-Al intermetallic compounds porous materials were fabricated with reactively synthesizing. The fabrication strategies allow us to precisely control the dimension of aperture, thus enabling us to load different amounts of drugs and control the release rates. In this work we have fabricated Ti-Al intermetallic compounds porous material with 40 similar to 200 nm and 2 similar to 5 mu m apertures. We have loaded these tubes with 180, 360 and 540 mu g of penicillin G. The penicillin G release kinetics from the apertures and its effect on Staphylococcus aureus ssp. anaerobius (S. aureus) adhesion were investigated. Further, an osteosarcoma cell line called MG-63 was cultured on penicillin G-loaded aperture to evaluate the effect of Ti-Al intermetallic compounds porous material on cell functionality. Our results indicate that we can effectively fill the apertures with the drug and the drug eluting apertures significantly reduced bacterial adhesion on the surface. Also, there is enhanced MG-63 differentiation on apertures filled with penicillin G.
语种：
英文
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逆向案例教学法在分析化学教学中的应用
作者：
肖锡林;廖力夫;林英武;聂长明;梁俊;...
期刊：
化工高等教育 ,2015年32(4):74-78 ISSN：1000-6168
作者机构：
南华大学化学化工学院,湖南衡阳,421001;[刘文娟; 聂长明; 梁俊; 林英武; 肖锡林; 王平; 廖力夫] 南华大学
关键词：
逆向案例;分析化学;教学方法
摘要：
该文采用便利取样法,选取2013级教学一班81名同学作为试验组,教学二班85名同学作为对照组。试验组采用逆向案例教学法施教,对照组采用传统教学法施教。教学方法的优劣通过比较测试成绩、调查学生对逆向案例教学方法的感受来评价。结果表明,应用逆向案例教学法学生的期中、期末成绩高于传统教学法的学生,并且学生认为逆向案例教学法可以提高学习兴趣和主动参与教学过程的积极性。在此基础上,该文结合逆向案例教学方法在分析化学课程教学中的应用及效果,提出了几点思考,认为逆向案例教学法教学效果较为理想,可以在更多的课程教学中进行推广。
语种：
中文
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Resonance light scattering detection of fructose bisphosphates using uranyl-salophen complex-modified gold nanoparticles as optical probe
作者：
Li, Shijun;Liao, Lifu* ;Wu, Rurong;Yang, Yanyan;Xu, Li;...
期刊：
Analytical and Bioanalytical Chemistry ,2015年407(29):8911-8918 ISSN：1618-2642
通讯作者：
Liao, Lifu
作者机构：
[Wu, Rurong; Xu, Li; Yang, Yanyan; Li, Shijun; Xiao, Xilin; Liao, Lifu; Nie, Changming] Univ South China, Coll Chem & Chem Engn, Hengyang 421001, Hunan, Peoples R China.
通讯机构：
[Liao, Lifu] U;Univ South China, Coll Chem & Chem Engn, Hengyang 421001, Hunan, Peoples R China.
关键词：
Fructose bisphosphates;Resonance light scattering;Uranyl;Salophen;Gold nanoparticles
摘要：
In this paper, we report a resonance light scattering (RLS) method for the determination of fructose bisphosphates (FBPs) in water solution using fructose 1,6-bisphosphate (F-1,6-BP) as a model analyte without the procedure of extracting target analyte. The method used a type of modified gold nanoparticles (GNPs) as optical probe. The modified GNPs are uranyl-salophen-cysteamine-GNPs (U-Sal-Cy-GNPs) which are obtained through the acylation reaction of carboxylated salophen with cysteamine-capped GNPs (Cy-GNPs) to form Sal-Cy-GNPs and then the chelation reaction of uranyl with tetradentate ligand salophen in the Sal-Cy-GNPs. A FBP molecule is used easily to connect two U-Sal-Cy-GNPs to cause the aggregation of the GNPs by utilizing the specific affinity of uranyl-salophen complex to phosphate group, resulting in the production of strong RLS signal from the system. The amount of FBPs can be determined through detecting the RLS intensity change of the system. A linear range was found to be 2.5 to 75 nmol/L with a detection limit of 0.91 nmol/L under optimal conditions. The method has been successfully used to determine FBPs in real samples with the recoveries of 96.5-103.5 %. © 2015 Springer-Verlag Berlin Heidelberg.
语种：
英文
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Adsorption of U(Ⅵ) by amino-functionalized magnetic ordered mesoporous silica
作者：
Guo Wenlu;Yuan Liyong;Nie Changming;Wei-Qun Shi
作者机构：
[Guo Wenlu; Yuan Liyong; Nie Changming; Wei-Qun Shi] Institute of High Energy Physics,Chinese Academy of Sciences;[Guo Wenlu; Yuan Liyong; Nie Changming; Wei-Qun Shi] School of Chemistry and Chemical Engineering,University of South China
会议名称：
中国化学会第九届全国无机化学学术会议
会议时间：
2015-01-01
会议地点：
中国江西南昌
会议论文集名称：
中国化学会第九届全国无机化学学术会议论文集-L能源材料化学
摘要：
Uranium, as a predominant fuel ingredient for most nuclear reactors, is the key element in nuclear fuel cycle. Moreover, its chemical and physiological toxicity can be never negligible when it is open
语种：
英文
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Uranyl-cytochrome b_5 interaction regulated by surface mutations and cytochrome c
作者：
Sun Mei-Hui;Du Ke-Jie;Nie Chang-Ming;Wen Ge-bo;Lin Ying-Wu*
期刊：
核技术(英文版) ,2015年26(5):050303-1-050303-4 ISSN：1001-8042
通讯作者：
Lin Ying-Wu
作者机构：
[Lin Ying-Wu; Sun Mei-Hui; Nie Chang-Ming; Du Ke-Jie] Univ South China, Sch Chem & Chem Engn, Hengyang 421001, Peoples R China.;[Wen Ge-bo; Lin Ying-Wu] Univ South China, Lab Prot Struct & Funct, Hengyang 421001, Peoples R China.
通讯机构：
[Lin Ying-Wu] U;Univ South China, Sch Chem & Chem Engn, Hengyang 421001, Peoples R China.
关键词：
Uranium;Cytochrome;Heme protein;Protein-protein interaction;Fluorescence
摘要：
Understanding uranium-protein interaction is important for revealing the mechanism of uranyl ion (UO_2~(2+)) toxicity. In this study, we investigated the interaction between UO_2~(2+) and a quadruple mutant of cytochrome b5 (E44/48/56A/D60A cyt b_5, namely 4A cyt b_5) by spectroscopic approaches. The four mutated negatively-charged surface residues of cyt b_5 have been considered to be the interactive sites with cytochrome c (cyt c). Also, we studied the interaction between UO_1~(2+) and the protein-protein complex of 4A cyt b5-cyt c. The results were compared to the interaction between UO_2~(2+) and cyt b5, and the interaction between cyt c and cyt b_5-cyt c complex, from previous studies. It was found that the interaction of UO_2~(2+)-cyt b_5, i.e., uranyl ion binding to cyt b_5 surface at Glu37 and Glu43 as previously proposed by molecular modeling, is regulated by both surface mutations of cyt b_5 and its interacting protein partner cyt c. These provide valuable information on metal-protein-protein interactions and clues for understanding the mechanism of uranyl toxicity.
语种：
英文
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基于信息技术与化学制药学科有效整合的教师三维能力结构模型思考
作者：
聂长明;廖力夫;林英武;肖新荣;谭倪;...
期刊：
药学教育 ,2015年31(4):28-31 ISSN：1007-3531
作者机构：
南华大学化学化工学院 衡阳 421001;[谭倪; 聂长明; 肖新荣; 肖锡林; 林英武; 廖力夫] 南华大学
关键词：
信息技术;化学制药;有效整合;三维能力结构
摘要：
信息技术给现代高等教育赋予了全新定义和空间. 从化学制药学科教师的信息素养与能力(X轴向)、教学设计能力(Y轴向)、教学实施能力(Z轴向)三维结构分析和建立实施信息技术与化学制药学科有效整合对教师的"三维能力结构"模型要求. 整合效果( IE)主要由教师的X、Y、Z"三维能力结构"协同决定,即IE=ILA+TDA+TIA,为信息技术与其他学科整合过程中的教师能力结构建设提供参考.
语种：
中文
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How a novel tyrosine-heme cross-link fine-tunes the structure and functions of heme proteins: A direct comparitive study of L29H/F43Y myoglobin
作者：
Yan, Dao-Jing;Yuan, Hong;Li, Wei;Xiang, Yu;He, Bo;...
期刊：
DALTON TRANSACTIONS ,2015年44(43):18815-18822 ISSN：1477-9226
通讯作者：
Lin, Ying-Wu
作者机构：
[Nie, Chang-Ming; Lin, Ying-Wu; He, Bo; Yan, Dao-Jing] Univ South China, Sch Chem & Chem Engn, Hengyang 421001, Peoples R China.;[Yuan, Hong; Li, Wei; Tan, Xiangshi] Fudan Univ, Shanghai Key Lab Chem Biol Prot Res, Dept Chem, Shanghai 200433, Peoples R China.;[Yuan, Hong; Li, Wei; Tan, Xiangshi] Fudan Univ, Inst Biomed Sci, Shanghai 200433, Peoples R China.;[Xiang, Yu] Tsinghua Univ, Dept Chem, Beijing 100084, Peoples R China.;[Wen, Ge-Bo; Lin, Ying-Wu] Univ South China, Lab Prot Struct & Funct, Hengyang 421001, Peoples R China.
通讯机构：
[Lin, Ying-Wu] U;Univ South China, Sch Chem & Chem Engn, Hengyang 421001, Peoples R China.
摘要：
A heme-protein cross-link is a key post-translational modification (PTM) of heme proteins. Meanwhile, the structural and functional consequences of heme-protein cross-links are not fully understood, due to limited studies on a direct comparison of the same protein with and without the cross-link. A Tyr-heme cross-link with a C-O bond is a newly discovered PTM of heme proteins, and is spontaneously formed in F43Y myoglobin (Mb) between the Tyr hydroxyl group and the heme 4-vinyl group in vivo. In this study, we found that with an additional distal His29 introduced in the heme pocket, the double mutant L29H/F43Y Mb can form two distinct forms under different protein purification conditions, with and without a novel Tyr-heme cross-link. By solving the X-ray structures of both forms of L29H/F43Y Mb and performing spectroscopic studies, we made a direct structural and functional comparison in the same protein scaffold. It revealed that the Tyr-heme cross-link regulates the heme distal hydrogen-bonding network, and fine-tunes not only the spectroscopic and ligand binding properties, but also the protein reactivity. Moreover, the formation of the Tyr-heme cross-link in the double mutant L29H/F43Y Mb was investigated in vitro. This study addressed the key issue of how Tyr-heme cross-link fine-tunes the structure and functions of the heme protein, and provided a plausible mechanism for the formation of the newly discovered Tyr-heme cross-link. © The Royal Society of Chemistry 2015.
语种：
英文
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Nonplanar Organic Sensitizers Featuring a Tetraphenylethene Structure and Double Electron-Withdrawing Anchoring Groups
作者：
Zhang, Fangshuai;Fan, Jie;Yu, Huijuan;Ke, Zhuofeng;Nie, Changming;...
期刊：
JOURNAL OF ORGANIC CHEMISTRY ,2015年80(18):9034-9040 ISSN：0022-3263
通讯作者：
Kuang, Daibin
作者机构：
[Fan, Jie; Kuang, Daibin; Zhang, Fangshuai; Ke, Zhuofeng; Yu, Huijuan; Su, Chengyong; Shao, Guang] Sun Yat Sen Univ, Sch Chem & Chem Engn, Guangzhou 510275, Guangdong, Peoples R China.;[Zhang, Fangshuai; Nie, Changming] Univ South China, Sch Chem & Chem Engn, Hengyang 421001, Peoples R China.
通讯机构：
[Kuang, Daibin] S;Sun Yat Sen Univ, Sch Chem & Chem Engn, Guangzhou 510275, Guangdong, Peoples R China.
摘要：
Two metal-free organic sensitizers containing two N,N-diethylaniline (DEA) moieties and a twisted 1,1,2,2-tetraphenylethene (TPE) structure, dye SD with one anchoring group and dye DD with two anchoring groups, were synthesized and applied in dye-sensitized solar cells (DSSCs). The introduction of a nonplanar TPE structure was used to form a series of propeller-like structures and reduce the tendency of dyes to randomly aggregate on TiO2 surface, but without importing an aggregation-induced emission (AIE) property. The thermal stabilities, UV-vis absorption spectra, electrochemical properties, and photovoltaic parameters of DSSCs with these two dyes were systematically studied and compared with each other. The overall conversion efficiencies (eta) of 4.56% for dye SD and 6.08% for dye DD were obtained under AM 1.5 G irradiation.
语种：
英文
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基础化学课程建设改革探索
作者：
廖力夫;肖锡林;林英武;聂长明
期刊：
当代教育理论与实践 ,2015年(4):52-54 ISSN：1674-5884
作者机构：
南华大学 化学化工学院,湖南 衡阳,421001;[聂长明; 林英武; 肖锡林; 廖力夫] 南华大学
关键词：
地方高校;基础化学;教学改革
摘要：
基础化学是医学生的一门重要基础课程。以地方高校基础化学教学特点和长期教学实践为基础,为适应高等教育大众化和创新型人才培养的要求,从地方高校基础化学课程教学内容、课堂教学及实践教学等环节的改革和创新入手,突出医学教育的特点,培养医学生从化学角度理解医学问题的能力,为地方培养高素质医学人才。
语种：
中文
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基于社会需求导向下化工类专业人才培养与企业创新互动机制研究
作者：
彭国文;聂长明;王延飞;阳鹏飞;陈仲清
期刊：
化工高等教育 ,2014年31(3):12-13,54 ISSN：1000-6168
作者机构：
南华大学化学化工学院,湖南衡阳,421001;[聂长明; 彭国文; 王延飞; 阳鹏飞; 陈仲清] 南华大学
关键词：
社会需求;化工人才;人才培养;企业创新
摘要：
社会需求是高等教育的原动力。本文在调查、分析的基础上,提出面向社会需求的教育新模型,并在校企合作的平台搭建、教学改革与实施、人才培养模式、实践教学等诸多方面进行广泛地研究。同时,还对构建校企协同合作培养人才的新模式,培养满足社会需求的高素质复合型技术人才进行了初步探索。
语种：
中文
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Resonance light scattering for detecting fluoride ions based on the formation of a uranyl coordination supramolecular polymer
作者：
Shen, Xing;Liao, Lifu* ;Chen, Lin;Xu, Canhui;He, Yunfei;...
期刊：
ANALYTICAL METHODS ,2014年6(13):4818-4822 ISSN：1759-9660
通讯作者：
Liao, Lifu
作者机构：
[Xu, Canhui; Chen, Lin; Xiao, Xilin; Liao, Lifu; Nie, Changming; Lin, Yingwu; Shen, Xing; He, Yunfei] Univ South China, Coll Chem & Chem Engn, Hengyang 421001, Hunan, Peoples R China.
通讯机构：
[Liao, Lifu] U;Univ South China, Coll Chem & Chem Engn, Hengyang 421001, Hunan, Peoples R China.
摘要：
A resonance light scattering (RLS) method for the detection of fluoride ions in aqueous solution is reported in this paper. Uranyl-bis-salophen (UBS), a binuclear uranyl complex prepared by the reaction of uranyl with a ditopic tetradentate Schiff base ligand bis-salophen, was used for the experiment. It is found that after UBS self-assembled with pyrophosphate to form a uranyl coordination supramolecular polymer (SP), the system produced a strong RLS signal. When fluoride ions coexisted with UBS, the formation of SP was inhibited due to which the fluoride ions occupied the coordination sites in UBS. This resulted in RLS quenching. Based on these findings, we established a RLS method for the detection of fluoride ions in aqueous solution. Under optimal conditions, the linear range was found to be 0.005 to 1.5 nmol mL-1 with a detection limit of 0.0028 nmol mL-1. The method was applied to determine fluoride ions in environmental water samples with relative standard deviations of 1.3 to 3.1% and recoveries of 98.7-101.3%. © the Partner Organisations 2014.
语种：
英文
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Computational insight into nitration of human myoglobin
作者：
Lin, Ying-Wu* ;Shu, Xiao-Gang;Du, Ke-Jie;Nie, Chang-Ming;Wen, Ge-Bo
期刊：
Computational Biology and Chemistry ,2014年52:60-65 ISSN：1476-9271
通讯作者：
Lin, Ying-Wu
作者机构：
[Nie, Chang-Ming; Shu, Xiao-Gang; Lin, Ying-Wu; Du, Ke-Jie] Univ South China, Sch Chem & Chem Engn, Hengyang 421001, Peoples R China.;[Wen, Ge-Bo; Lin, Ying-Wu] Univ South China, Lab Prot Struct & Funct, Hengyang 421001, Peoples R China.
通讯机构：
[Lin, Ying-Wu] U;Univ South China, Sch Chem & Chem Engn, Hengyang 421001, Peoples R China.
关键词：
Heme active site;Heme proteins;Internal cavity;MD simulation;Nitration
摘要：
Protein nitration is an important post-translational modification regulating protein structure and function, especially for heme proteins. Myoglobin (Mb) is an ideal protein model for investigating the structure and function relationship of heme proteins. With limited structural information available for nitrated heme proteins from experiments, we herein performed a molecular dynamics study of human Mb with successive nitration of Tyr103, Tyr146, Trp7 and Trp14. We made a detailed comparison of protein motions, intramolecular contacts and internal cavities of nitrated Mbs with that of native Mb. It showed that although nitration of both Tyr103 and Tyr146 slightly alters the local conformation of heme active site, further nitration of both Trp7 and Trp14 shifts helix A apart from the rest of protein, which results in altered internal cavities and forms a water channel, representing an initial stage of Mb unfolding. The computational study provides an insight into the nitration of heme proteins at an atomic level, which is valuable for understanding the structure and function relationship of heme proteins in non-native states by nitration. © 2014 Elsevier Ltd. All rights reserved.
语种：
英文
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Quantum topological method studies on the thermodynamic properties of polychlorinated phenoxazines
作者：
Xiao, Fangzhu;Peng, Guowen* ;Nie, Changming;Wu, Yaxin;Dai, Yimin
期刊：
Journal of Molecular Structure ,2014年1074:679-686 ISSN：0022-2860
通讯作者：
Peng, Guowen
作者机构：
[Xiao, Fangzhu] Univ South China, Sch Publ Hlth, Hengyang 421001, Peoples R China.;[Nie, Changming; Peng, Guowen; Wu, Yaxin] Univ South China, Sch Chem & Chem Engn, Hengyang 421001, Peoples R China.;[Nie, Changming] Univ Toulouse 3, Lab Quantum Chem & Phys, F-31077 Toulouse, France.;[Dai, Yimin] Changsha Univ Sci & Technol, Sch Chem & Biol Engn, Hunan Prov Key Lab Mat Protect Elect Power & Tran, Changsha 410004, Hunan, Peoples R China.
通讯机构：
[Peng, Guowen] U;Univ South China, Sch Chem & Chem Engn, Hengyang 421001, Peoples R China.
关键词：
Polychlorinated phenoxazines;Thermodynamic properties;Quantum topological index;Equilibrium electro-negativity;QSPR
摘要：
The novel quantum topological indices PY<inf>1,2</inf>were derived from molecular structure combined with the effect of atom space, the character of bonding atoms (such as equilibrium electro-negativity) and the branching effect between the atoms. The quantitative structure-property relationships (QSPRs) were proposed between PY<inf>1,2</inf>and the thermodynamic properties (<inf>f</inf>H<sup>θ</sup>, <inf>f</inf>G<sup>θ</sup>and Δ<inf>f</inf>G<inf>R</inf><sup>θ</sup>) of phenoxazine (Phx) and 135 kinds of polychlorinated phenoxazines (PCPXs), by Multiple linear regression (MLR) analysis method. The high-quality prediction models were evidenced by the correlation coefficient R, the standard error of estimate S, the Fisher statistic value and the cross-validated correlation coefficient RCV. With the new QSPR model, we are able to predict a wide range of thermodynamic properties of an extensive number of molecules. And the model is statistically significant and shows good stability for data variation as tested by the leave-one-out cross-validation (LOO-CV). ©2014 Published by Elsevier B.V.
语种：
英文
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Electrochemical behavior of uranyl in ionic liquid 1-butyl-3-methylimidazolium chloride mixture with water
作者：
Tan, Xu-Feng;Yuan, Li-Yong;Nie, Chang-Ming* ;Lui, Kui;Chai, Zhi-Fang;...
期刊：
Journal of Radioanalytical and Nuclear Chemistry ,2014年302(1):281-288 ISSN：0236-5731
通讯作者：
Nie, Chang-Ming
作者机构：
[Nie, Chang-Ming; Tan, Xu-Feng] Univ South China, Sch Chem & Chem Engn, Hengyang 421001, Peoples R China.;[Lui, Kui; Tan, Xu-Feng; Yuan, Li-Yong; Shi, Wei-Qun] Chinese Acad Sci, Inst High Energy Phys, Key Lab Nucl Radiat & Nucl Energy Technol, Beijing 100049, Peoples R China.;[Chai, Zhi-Fang] Soochow Univ, Sch Radiol & Interdisciplinary Sci, Suzhou 215123, Peoples R China.
通讯机构：
[Nie, Chang-Ming] U;Univ South China, Sch Chem & Chem Engn, Hengyang 421001, Peoples R China.
关键词：
Ionic liquid;Cyclic voltammetry;Electrochemical behavior;Uranyl
摘要：
Electrochemical behaviors of U(VI) in 1-butyl-3-methylimidazolium chloride (C4MimCl) with various water contents investigated by chronopotentiometry and cyclic voltammetry. The electrochemical reduction of U(VI) was identified to follow two processes: a lower valence intermediate U(V) was initially formed at the potential of ca. -0.2 V(vs. Ag wire). Then, further deposition of UO2 was followed at around -0.8 V. Little amount of water (1–4 wt%) in C4MimCl, however, has an effect on the U(VI) reduction by changing the current density of the redox reaction and the diffusion coefficient of U(VI) in C4MimCl. The deposited product by potentiostatic electrolysis on the surface of stainless steel electrode was characterized by the scanning electron microscopy (SEM), energy dispersive X-ray (EDX) and X-ray diffraction (XRD) methods. Although the electrodeposited black film was amorphous, the electrochemical reduced product of U(VI) can be still confirmed to be UO2 by XRD after the crystallization of the amorphous deposits at 1,073 K in nitrogen atmosphere. © 2014,Akadémiai Kiadó, Budapest, Hungary
语种：
英文
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三种铀酰-Salophen配合物分子结构与振动光谱的理论研究
作者：
肖云霞;聂长明;李小龙;罗娟;张方帅
期刊：
核化学与放射化学 ,2014年36(2):97-103 ISSN：0253-9950
通讯作者：
Nie, C.-M.
作者机构：
南华大学 化学化工学院,湖南 衡阳,421001;[聂长明; 张方帅; 肖云霞; 罗娟; 李小龙] 南华大学
通讯机构：
College of Chemistry and Chemical Engineering, University of South China, China
关键词：
密度泛函理论;铀酰-Salophen配合物;分子结构;红外光谱;热力学性质;化学位移
摘要：
采用密度泛函理论(DFT)中的B3LYP方法,在6-31G、6-31G~(**)、6-311G~(**)三种基组水平上,对三种铀酰-Salophen配合物的分子结构进行优化,计算了它们的振动频率和化学位移,并对得到的几何构型、电子结构、红外光谱、热力学性质和化学位移进行了讨论分析。
语种：
中文
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亚洲玉米螟地理种群不同食物及温度下实验种群的蛹重研究
作者：
程鲁雪;和淑琪;严乃胜;李正跃;陈斌;...
期刊：
环境昆虫学报 ,2014年21(3):322-326 ISSN：1674-0858
作者机构：
云南农业大学植物保护学院,农业生物多样性与病虫害控制教育部重点实验室,农业生物多样性应用技术国家工程研究中心,昆明650201;云南农业大学基础与信息工程学院,昆明650201;[程鲁雪; 和淑琪; 严乃胜; 李正跃; 陈斌; 张立敏] 云南农业大学
关键词：
亚洲玉米螟;蛹重;地理种群;实验种群
摘要：
为明确亚洲玉米螟Ostrinia furnacalis (Guenée)不同地理自然种群及不同食物和不同温度条件下实验种群蛹重的差异,对云南不同玉米种植区亚洲玉米螟自然种群、不同食料及不同温度下饲养的实验种群的雌、雄蛹重进行了研究.结果表明:云南昭通、会泽、宣威、寻甸、建水、玉溪、元江和陇川县不同地理种群雌蛹和雄蛹重量之间均存在显著性差异,从同一地理来源的雌蛹、雄蛹重量来看,雌蛹重量明显大于雄蛹重量.室内21℃、25℃和29 ℃下饲养的实验种群雌蛹重量均显著高于雄蛹重量,21 ℃与25℃下雌蛹、雄蛹重量大于29 ℃的雌蛹、雄蛹重量,而21℃和25℃下实验种群雌、雄蛹重无显著差异.室内常温条件下人工饲料饲养种群与玉米秆饲养种群雌蛹、雄蛹重量无显著差异.综合以上结果,亚洲玉米螟雌蛹重大于雄蛹,且随着不同地理来源而存在一定差异,而室内利用人工饲料和玉米秆饲养种群的雌、雄蛹重间无显著差异,在21-25℃条件下种群雌雄蛹重量大于29℃条件下雌雄蛹重量,该结果将为亚洲玉米螟对环境的适应性及其机理研究提供理论依据.
语种：
中文
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A resonance light scattering method for the determination of uranium based on a water-soluble salophen and oxalate
作者：
Chen, Lin;Liao, Lifu* ;Shen, Xing;He, Yunfei;Xu, Canhui;...
期刊：
Journal of Radioanalytical and Nuclear Chemistry ,2014年301(3):863-869 ISSN：0236-5731
通讯作者：
Liao, Lifu
作者机构：
[Xu, Canhui; Chen, Lin; Xiao, Xilin; Liao, Lifu; Nie, Changming; Lin, Yingwu; Shen, Xing; He, Yunfei] Univ South China, Coll Chem & Chem Engn, Hengyang 421001, Hunan, Peoples R China.
通讯机构：
[Liao, Lifu] U;Univ South China, Coll Chem & Chem Engn, Hengyang 421001, Hunan, Peoples R China.
会议名称：
Biennial Symposium on Nuclear and Radiochemistry
会议时间：
FEB 19-23, 2013
会议地点：
Jabalpur, INDIA
会议主办单位：
[Chen, Lin;Liao, Lifu;Shen, Xing;He, Yunfei;Xu, Canhui;Xiao, Xilin;Lin, Yingwu;Nie, Changming] Univ South China, Coll Chem & Chem Engn, Hengyang 421001, Hunan, Peoples R China.
关键词：
Uranium;Resonance light scattering;Water-soluble salophen;Oxalate
摘要：
A resonance light scattering (RLS) method for the direct detection of uranium (VI) or uranyl in aqueous solution without separation procedure has been reported in this paper. Sulfo-salophen, a water-soluble tetradentate Schiff base ligand of uranyl, reacted with uranyl to form a complex. The complex reacted further with oxalate to form supramolecular dimer with large molecular volume, resulting in a production of strong RLS signal. The amount of uranium (VI) was detected through measuring the RLS intensity. A linear range was found to be 0.2-30.0 ng/mL under optimal conditions with a detection limit of 0.15 ng/mL. The method has been applied to determine uranium (VI) in environmental water samples with the relative standard deviations of less than 5 % and the recoveries of 98.8-105.8 %. The present technique is suitable for the assay of uranium (VI) in environmental water samples collected from different sources. © 2014 Akadémiai Kiadó.
语种：
英文
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产业转型背景下的高等工程教育培养模式探索
作者：
胡永刚;王宏青;聂长明
期刊：
广州化工 ,2014年42(8):229-230,236 ISSN：1001-9677
作者机构：
南华大学化学化工学院,湖南衡阳,421001;[聂长明; 王宏青; 胡永刚] 南华大学
关键词：
产业转型;工程教育;培养模式;无机非金属材料
摘要：
以无机非金属材料专业为例,指出了当前工程教育人才培养模式的一些不足。针对节能环保及产业转型对无机非金属材料专业发展提出的新要求,变革当前培养模式:通过多元化培养方案,完善教材体系,强化专业选修课的教学,实现学生的层次化个性发展;通过科技孵化平台/实验教学平台/创新平台/重点实验室的建设,满足实习、实践教学的需要,为产业转型背景下的多元化高级技术人才培养提供良好的实验、实习、实践教学条件和环境。
语种：
中文
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基于卓越工程师培养的“分析化学”教学改革探讨
作者：
廖力夫;肖锡林;王宏青;聂长明;林英武;...
期刊：
中国电力教育 ,2014年(23):86-87 ISSN：1007-0079
作者机构：
南华大学化学化工学院,湖南 衡阳,421001;[刘文娟; 聂长明; 王宏青; 林英武; 肖锡林; 王平; 廖力夫] 南华大学
关键词：
地方高校;分析化学;小组讨论式教学;双语教学;卓越工程师
摘要：
“分析化学”是化学工程与工艺等专业的必修学科基础课程，在工科人才培养中起着重要作用。目前中外教育交流日益频繁，教育国际化趋势明显，如何合理借鉴海外先进教学经验，是一个值得探索的课题。以所在地方高校为例，坚持以学生为中心，进行“分析化学”小组讨论式双语教学模式改革，将课内与课外、科学与人文、教学与研究结合起来，形成独具特色的卓越工程师人才培养模式，满足时代发展的需求，为实现国际化人才培养目标打下良好基础。
语种：
中文
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A spectroscopic study of uranyl-cytochrome b5/cytochrome c interactions
作者：
Sun, Mei-Hui;Liu, Shuang-Quan;Du, Ke-Jie;Nie, Chang-Ming;Lin, Ying-Wu*
期刊：
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY ,2014年118:130-137 ISSN：1386-1425
通讯作者：
Lin, Ying-Wu
作者机构：
[Nie, Chang-Ming; Lin, Ying-Wu; Sun, Mei-Hui; Du, Ke-Jie] Univ South China, Sch Chem & Chem Engn, Hengyang 421001, Peoples R China.;[Liu, Shuang-Quan] Univ South China, Affiliated Hosp 1, Dept Clin Lab, Hengyang 421001, Peoples R China.
通讯机构：
[Lin, Ying-Wu] U;Univ South China, Sch Chem & Chem Engn, Hengyang 421001, Peoples R China.
关键词：
Uranium;Heme protein;Protein-protein interaction;Fluorescence;Peroxidase
摘要：
Uranium is harmful to human health due to its radiation damage and the ability of uranyl ion (UO22+) to interact with various proteins and disturb their biological functions. Cytochrome b<inf>5</inf> (cyt b<inf>5</inf>) is a highly negatively charged heme protein and plays a key role in mediating cytochrome c (cyt c) signaling in apoptosis by forming a dynamic cyt b <inf>5</inf>-cyt c complex. In previous molecular modeling study in combination with UV-Vis studies, we found that UO22+ is capable of binding to cyt b <inf>5</inf> at surface residues, Glu37 and Glu43. In this study, we further investigated the structural consequences of cyt b<inf>5</inf> and cyt c, as well as cyt b<inf>5</inf>-cyt c complex, upon uranyl binding, by fluorescence spectroscopic and circular dichroism techniques. Moreover, we proposed a uranyl binding site for cyt c at surface residues, Glu66 and Glu69, by performing a molecular modeling study. It was shown that uranyl binds to cyt b<inf>5</inf> (K<inf>D</inf> = 10 μM), cyt c (K<inf>D</inf> = 87 μM), and cyt b <inf>5</inf>-cyt c complex (K<inf>D</inf> = 30 μM) with a different affinity, which slightly alters the protein conformation and disturbs the interaction of cyt b<inf>5</inf>-cyt c complex. Additionally, we investigated the functional consequences of uranyl binding to the protein surface, which decreases the inherent peroxidase activity of cyt c. The information of uranyl-cyt b <inf>5</inf>/cyt c interactions gained in this study likely provides a clue for the mechanism of uranyl toxicity. ©2013 Elsevier B.V. All rights reserved.
语种：
英文
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