摘要:
PIWI proteins have a strong correlation with PIWI-interacting RNAs (piRNAs), which are significant in development and reproduction of organisms. Recently, emerging evidences have indicated that apart from the reproductive function, PIWI/piRNAs with abnormal expression, also involve greatly in varieties of human cancers. Moreover, human PIWI proteins are usually expressed only in germ cells and hardly in somatic cells, so the abnormal expression of PIWI proteins in different types of cancer offer a promising opportunity for precision medicine. In this review, we discussed current researches about the biogenesis of piRNA, its epigenetic regulatory mechanisms in human cancers, such as N6-methyladenosine (m6A) methylation, histone modifications, DNA methylation and RNA interference, providing novel insights into the markers for clinical diagnosis, treatment and prognosis in human cancers.
摘要:
Cardiovascular disease is a growing major global public health problem. Oxidative stress is regarded as one of the key regulators of pathological physiology, which eventually leads to cardiovascular disease. However, mechanisms by which FGF-2 rescues cells from oxidative stress damage in cardiovascular disease is not fully elucidated. Herein this study was designed to investigate the protective effects of FGF-2 in H2O2-induced apoptosis of H9c2 cardiomyocytes, as well as the possible signaling pathway involved. Apoptosis of H9c2 cardiomyocytes was induced by H2O2 and assessed using methyl thiazolyl tetrazolium assay, Hoechst, and TUNEL staining. Cells were pretreated with PI3K/Akt inhibitor LY294002 to investigate the possible PI3K/Akt pathways involved in the protection of FGF-2. The levels of p-Akt, p-FoxO3a, and Bim were detected by immunoblotting. Stimulation with H2O2 decreased the phosphorylation of Akt and FoxO3a, and induced nuclear localization of FoxO3a and apoptosis of H9c2 cells. These effects of H2O2 were abrogated by pretreatment with FGF-2. Furthermore, the protective effects of FGF-2 were abolished by PI3K/Akt inhibitor LY294002. In conclusion, our data suggest that FGF-2 protects against H2O2-induced apoptosis of H9c2 cardiomyocytes via activation of the PI3K/Akt/FoxO3a pathway.
作者:
Tang, Ya Ling;Jiang, Jian Hong;Wang, Shuang;Liu, Zhu;Tang, Xiao Qing;...
期刊:
PLOS ONE,2015年10(4):e0123685- ISSN:1932-6203
通讯作者:
Gu, Hong-Feng
作者机构:
[Yang, Yong-Zong; Gu, Hong-Feng; Wang, Shuang; Tang, Ya Ling; Peng, Juan] Univ South China, Inst Cardiovasc Dis, Key Lab Arteriosclerol Hunan Prov, Hengyang 421001, Peoples R China.;[Liu, Zhu; Jiang, Jian Hong] Univ South China, Chuanshan Coll, Hengyang 421001, Peoples R China.;[Gu, Hong-Feng; Tang, Xiao Qing] Univ South China, Dept Physiol, Hengyang 421001, Peoples R China.;[Gu, Hong-Feng; Tang, Xiao Qing] Univ South China, Inst Neurosci, Hengyang 421001, Peoples R China.
通讯机构:
[Gu, Hong-Feng] U;Univ South China, Inst Cardiovasc Dis, Key Lab Arteriosclerol Hunan Prov, Hengyang 421001, Peoples R China.
关键词:
Small interfering RNAs;Atherosclerosis;Transcription factors;Inflammation;Cytokines;Aorta;Immune receptor signaling;Stress signaling cascade
摘要:
Objective Chronic stress is an important risk factor for atherosclerotic diseases. Our previous studies have shown that chronic unpredictable mild stress (CUMS) accelerates atherosclerosis and up-regulates TLR4/NF-kappa B expression in apoE(-/-) mice. However, TLR4/NF-kappa B signaling whether directly contributes to the development of atherosclerosis in CUMS mice is unclear. We hypothesized that the interference of TLR4/NF-kappa B can ameliorate CUMS-induced inflammation and atherosclerosis in apoE(-/-) mice. Methods ApoE(-/-) mice were exposed to 12 weeks CUMS. Ad-siRNA TLR4 was given by tail vein injection (10 mu l/mouse, every 5 days), and PDTC (an inhibitor of NF-kappa B) was given by intraperitoneal injection (60 mg/kg, once a day). Plasma corticosterone concentrations were determined by solid-phase I-125 radioimmunoassay. Atherosclerosis lesions in aortic sinuses were evaluated and quantified by IMAGEPRO PLUS. Western blotting was used to detect the expression of TLR4, NF-kappa B, and IL-1 beta in aortas of the mice. Plasma lipid profiles, IL-1 beta, TNF-alpha, and MCP-1 were measured by ELISA. Results Our results indicated that CUMS apoE(-/-) mice treatment with siRNA TLR4 significantly decreased atherosclerosis and down-regulated TLR4, NF-kappa B, and inflammatory cytokines. PDTC also remarkably reduced atherosclerosis and the levels of IL-1 beta, TNF-alpha and MCP-1 in plasma. However, Treatment with siRNA TLR4 or PDTC had no effect on plasma corticosterone levels, and lipid profiles. Conclusions TLR4/NF-kappa B pathway may participate in CUMS-induced atherosclerosis through activation of proinflammatory cytokines in apoE(-/-) mice. Our data may provide a new potential therapeutic target for prevention of CUMS-induced atherosclerosis.