摘要：
Recently, microRNAs have been detected in serum and plasma, and circulating microRNA (miRNA) profiles have now been associated with many diseases such as cancers and heart disease, as well as altered physiological states. Because of their stability and disease resistance, circulation miRNAs appear to be an ideal material for biomarkers of diseases and physiological states in blood. However, the lack of a suitable internal reference gene (internal reference miRNA) has hampered research and application of circulating miRNAs. Currently, U6 and miR-16 are the most common endogenous controls in the research of miRNAs in tissues and cells. We performed microarray-based serum miRNA profiling on the serum of 20 nasopharyngeal carcinoma patients and 20 controls to detect the expressions of U6 and miRNAs. Profiling was followed by real-time quantitative Polymerase Chain Reaction (qPCR) in 80 patients (20 each with gastric cancer, nasopharyngeal carcinoma, colorectal cancer, and breast cancer) and 30 non-cancerous controls. qPCR was also performed to detect miRNAs in serum with repeated freezing and thawing. The results of microarray showed that with the exception of U6, Ct values of miR-16, miR-24, miR-142-3p, miR-19b and miR-192 in serum samples of nasopharyngeal carcinoma were greater than control samples. The results of 110 cases showed large fluctuations in U6 expression. The difference between the greatest and the least levels of expression was 3.29 for delta Ct values, and 1.23 for miR-16. The expressions of U6, miR-16 and miR-24 in serum subjected to different freeze-thaw cycles showed that U6 expression gradually decreased after 1, 2, and 4 cycles of freezing and thawing, while the expression of miR-16 and miR24 remained relatively stable. Collectively, our results suggested that U6 is unsuitable as an internal reference gene in the research of circulating miRNAs. (C) 2014 Elsevier Inc. All rights reserved.
摘要：
Previous studies have shown that melatonin is implicated in modulating learning and memory processing. Melatonin also exerts neuroprotective activities against A beta-induced injury in vitro and in vivo. Neu-P11 (piromelatine, N-(2-(5-methoxy-1H-indol-3-yl)ethyl)-4-oxo-4H-pyran-2-carboxamide) is a novel melatonin (MT1/MT2) receptor agonist and a serotonin 5-HT1A/1D receptor agonist recently developed for the treatment of insomnia. In the present study we firstly investigated whether Neu-P11 and melatonin enhance memory performance in the novel object recognition (NOR) task in rats, and then assessed whether Neu-P11 and melatonin improve neuronal and cognitive impairment in a rat model of Alzheimer' disease (AD) induced by intrahippocampal A beta((1-42)) injection. The results showed that a single morning or afternoon administration of Neu-P11 enhanced object recognition memory measured at 4 or 24 h after training. Melatonin was effective in the memory facilitating effects only when administered in the afternoon. Further results showed that intrahippocampal A beta((1-42)) injection resulted in hippocampal cellular loss, as well as decreased learning ability and memory in the Y maze and NOR tasks in rats. Neu-P11 but not melatonin attenuated cellular loss and cognitive impairment in the rat AD model. The current data suggest that Neu-P11 may serve as a novel agent for the treatment of AD. (C) 2013 Elsevier Inc. All rights reserved.
摘要：
Lipoprotein lipase (LPL) is a key enzyme in lipid metabolism and responsible for catalyzing lipolysis of triglycerides in lipoproteins. LPL is produced mainly in adipose tissue, skeletal and heart muscle, as well as in macrophage and other tissues. After synthesized, it is secreted and translocated to the vascular lumen. LPL expression and activity are regulated by a variety of factors, such as transcription factors, interactive proteins and nutritional state through complicated mechanisms. LPL with different distributions may exert distinct functions and have diverse roles in human health and disease with close association with atherosclerosis. It may pose a pro-atherogenic or an anti-atherogenic effect depending on its locations. In this review, we will discuss its gene, protein, synthesis, transportation and biological functions, and then focus on its regulation and relationship with atherosclerosis and potential underlying mechanisms. The goal of this review is to provide basic information and novel insight for further studies and therapeutic targets. (C) 2014 Elsevier Ireland Ltd. All rights reserved.
摘要：
Background: Accumulating evidence suggests that microRNA-590 (miR-590) has protective effects on cardiovascular diseases, but the mechanism is unknown. Interestingly, previous studies from our laboratory and others have shown that macrophage-derived lipoprotein lipase (LPL) might accelerate atherosclerosis by promoting lipid accumulation and inflammatory response. However, the regulation of LPL at the post-transcriptional level by microRNAs has not been fully understood. In this study, we explored whether miR-590 affects the expression of LPL and its potential subsequent effects on lipid accumulation and pro-inflammatory cytokine secretion in human THP-1 macrophages. Methods and results: Using bioinformatics analyses and dual-luciferase reporter assays, we found that miR-590 directly inhibited LPL protein and mRNA expression by targeting LPL 3'UTR. LPL Activity Assays showed that miR-590 reduced LPL activity in the culture media. Oil Red 0 staining and high-performance liquid chromatography assays showed that miR-590 had inhibitory effects on the lipid accumulation in human THP-1 macrophages. We also illustrated that miR-590 alleviated pro-inflammatory cytokine secretion in human THP-1 macrophages as measured by ELISA. With the method of small interfering RNA, we found that LPL siRNA can inhibit the miR-590 inhibitor-induced increase in lipid accumulation and secretion of pro-inflammatory cytokines in oxLDL-treated human THP-1 macrophages. Conclusions: MiR-590 attenuates lipid accumulation and pro-inflammatory cytokine secretion by targeting LPL gene in human THP-1 macrophages. Therefore, targeting miR-590 may offer a promising strategy to treat atherosclerotic cardiovascular diseases. (C) 2014 Elsevier B.V. and Societe francaise de biochimie et biologie Moleculaire (SFBBM). All rights reserved.
通讯机构：
[Tang, Chao-Ke] Univ S China, Inst Cardiovasc Res, Key Lab Atherosclerol Hunan Prov, Life Sci Res Ctr, Hengyang 421001, Hunan, Peoples R China.
关键词：
ATP-binding cassette transporter A1;Interleukin-12;Interleukin-18;Liver X receptor alpha;Nuclear factor-kappa B
摘要：
Background: Interleukin (IL)-18 and IL-12 synergize for the production of interferon (IFN)-gamma, which can downregulate ATP-binding cassette transporter A1 (ABCA1) expression. The aim of the present study was to investigate the effect of IL-18 and/or IL-12 on ABCA1 expression. Methods and Results: IL-18 combined with IL-12 decreased ABCA1 expression and cellular cholesterol efflux in THP-1 macrophage-derived foam cells, whereas IL-18 or IL-12 alone had no effect. IL-12 increased IL-18 receptor (IL-18R) expression, which was suppressed by small interfering RNA (siRNA) for signal transducer and activator of transcription 3. IL-18R but not IL-12 receptor siRNA completely reversed the effects of IL-18 and IL-12 on ABCA1 expression and cellular cholesterol efflux. Treatment with IL-18 plus IL-12 markedly augmented nuclear translocation of nuclear factor(NF)-kappa B but had no effect on expression and activity of liver X receptor a. IL-18 and IL-12 also significantly increased zinc finger protein 202 (ZNF202) levels and IFN-gamma secretion. Furthermore, siRNA for ZNF202 or IFN-gamma significantly impaired IL-18/IL-12-induced suppression of ABCA1, whereas NF-kappa B siRNA treatment blocked IL-18/IL-12' action on ZNF202 levels, IFN-gamma secretion, and ABCA1 expression. Conclusions: IL-18 and IL-12 together can decrease ABCA1 expression and cellular cholesterol efflux in THP-1 macrophage-derived foam cells through the IL-18R/NF-kappa B signaling pathway. (Circ J 2012; 76:1780-1791)
作者：
Xie, R-H;Liao, S.;Xie, H.;Guo, Y.;Walker, M.;Wen, S. W.*
期刊：
Journal of Epidemiology and Community Health (1979-),2011年65(8):722-726 ISSN：0143-005X
通讯作者：
Wen, S. W.
作者机构：
[Wen, S. W.; Guo, Y.; Walker, M.; Xie, R-H] Univ Ottawa, OMNI Res Grp, Dept Obstet & Gynecol, Fac Med, Ottawa, ON K1H 8L6, Canada.;[Wen, S. W.; Guo, Y.; Walker, M.; Xie, R-H] Ottawa Hosp Res Inst, Clin Epidemiol Program, Ottawa, ON, Canada.;[Xie, R-H] Huaihua Med Coll, Dept Nursing, Huaihua, Peoples R China.;[Wen, S. W.; Liao, S.; Guo, Y.] Cent S Univ, Sch Publ Hlth, Changsha, Hunan, Peoples R China.;[Xie, H.] Univ S China, Sch Nursing, Hengyang, Hunan, Peoples R China.
通讯机构：
[Wen, S. W.] Univ Ottawa, OMNI Res Grp, Dept Obstet & Gynecol, Fac Med, 501 Smyth Rd,Box 241, Ottawa, ON K1H 8L6, Canada.
摘要：
Objectives To assess the impact of prenatal and postnatal family support on the association between infant sex and postpartum depression (PPD). Design Prospective cohort study. Setting Pregnant women seen at Hunan Maternal and Infant Hospital, the First Affiliated and the Third Affiliated Hospitals of the Central South University in Changsha, Hunan, People's Republic of China from February to September 2007. Participants 534 Pregnant women who were consecutively recruited from the participating hospital during their prenatal visits at 30-32 weeks of gestation and who completed the 2 weeks postpartum survey, with no recorded major psychiatric disorders and obstetric and/or pregnancy complications. Main outcome measure PPD, which was defined as a score of 13 or higher of the Edinburgh Postnatal Depression Scale. Results Postnatal family support scores were much lower in women who gave birth to a female infant, and the OR of PPD was 3.67 (95% CI 2.31 to 5.84) for them as compared to women who gave birth to a male infant. After adjusting by postnatal support from all family members, husband and parents, the ORs of PPD for women who gave birth to a female infant decreased to 2.06 (95% CI 1.20 to 3.53), 2.89 (95% CI 1.76 to 4.77) and 2.20 (95% CI 1.28 to 3.77), respectively. Discussion Increased risk of PPD in Chinese women who gave birth to a female infant can be explained to large extent by inadequate or poor postpartum support from family members, particularly husband and parents.
摘要：
ATP-binding cassette transporter A1 (ABCA1) is critical in exporting cholesterol from macrophages and plays a protective role in the development of atherosclerosis. The purpose of this study was to investigate the effects of betulinic acid (BA), a pentacyclic triterpenoid, on ABCA1 expression and cholesterol efflux, and to further determine the underlying mechanism. BA promoted ABCA1 expression and cholesterol efflux, decreased cellular cholesterol and cholesterol ester content in LPS-treated macrophages. Furthermore, we found that BA promoted ABCA1 expression via down-regulation of miR-33s. The inhibition of LPS-induced NF-kappa B activation further decreased miR-33s expression and enhanced ABCA1 expression and cholesterol efflux when compared with BA only treatment. In addition, BA suppressed I kappa B phosphorylation, p65 phosphorylation and nuclear translocation, and the transcription of NF-kappa B-dependent related gene. Moreover, BA reduced atherosclerotic lesion size, miR-33s levels and NF-kappa B activation, and promoted ABCA1 expression in apoE(-/-) mice. Taken together, these results reveal a novel mechanism for the BA-mediated ABCA1 expression, which may provide new insights for developing strategies for modulating vascular inflammation and atherosclerosis.
摘要：
OBJECTIVE: To investigate awareness and knowledge of human papillomavirus (HPV) infection among high school students and to provide a basis for health education on HPV infection for high school students in China. STUDY DESIGN: A ques- awareness of tionnaire on HPV awareness and knowledge was adminis- provide the tered to 900 high school students in Xiangtan City of Hunan Province in China by layer cluster sampling. A total of 848 anonymous valid questionnaires were received from volunteers who cornpleted the questionnaire correctly. RESULTS: Only 10.1% had heard of HPV, and of those only 18.6% knew that HPV could lead to cervical cancer. Single factor analysis indicated that home address, age, grade, academic achievement, sex history, gender, father's education level and mother's education level were impact factors for HPV knowledge of high school students. Multiple regression analysis showed 4 independent risk factors associated with HPV knowledge: academic achievement, sex history, gender, and mother's education level. The limited knowledge came primarily frorri television and radio broadcasts (59.3%), the Internet (57.0%), parents (25.6%), medical workers (20.9%), and teachers (18.6%). CONCLUSION: High school students lack HPV people's knowledge, which is affectand CC can ed by multiple factors. Targeted health education of for targeted all sorts must be provided. Both schools and families are responsible for reinforcing HPV education provided to high school students.
通讯机构：
[Yin, Wei-Dong] Univ South China, Inst Cardiovasc Res, Key Lab Atherosclerol Hunan Prov, Hengyang 421001, Hunan, Peoples R China.
关键词：
miR-467b;LPL;Atherosclerosis;Apolipoprotein E knockout mice
摘要：
Atherosclerosis is a lipid disorder disease characterized by chronic blood vessel wall inflammation driven by the subendothelial accumulation of macrophages. Studies have shown that lipoprotein lipase (LPL) participates in lipid metabolism, but it is not yet known whether post-transcriptional regulation of LPL gene expression by microRNAs (miRNAs) occurs in vivo. Here, we tested that miR-467b provides protection against atherosclerosis by regulating the target gene LPL which leads to reductions in LPL expression, lipid accumulation, progression of atherosclerosis and production of inflammatory cytokines in apolipoprotein E knockout (apoE(-/-)) mice. Treatment of apoE(-/-) mice with intra-peritoneal injection of miR-467b agomir led to decreased blood plasma levels of total cholesterol (TC), triglyceride (TG), low density lipoprotein cholesterol (LDL-C), high density lipoprotein cholesterol (HDL-C), tumor necrosis factor-alpha (TNF-alpha), interleukin-6 (IL-6), IL-1 beta and monocyte chemotactic protein-1 (MCP-1). Using Western blots and real time PCR, we determined that LPL expression in aorta and abdominal cavity macrophages were significantly down-regulated in the miR-467b agomir group. Furthermore, systemic treatment with miR-467b antagomir accelerated the progression of atherosclerosis in the aorta of apoE(-/-) mice. The present study showed that miR-467b protects apoE(-/-) mice from atherosclerosis by reducing lipid accumulation and inflammatory cytokine secretion via downregulation of LPL expression. Therefore, targeting miR-467b may offer a promising strategy to treat atherosclerotic vascular disease. (C) 2013 Elsevier Inc. All rights reserved.
期刊：
European Journal of Oncology Nursing,2016年20:24-30 ISSN：1462-3889
通讯作者：
Chen, Yongyi;He, Pingping
作者机构：
[Chen, Yongyi; Tang, Xinhui; Liu, Xiangyu; Wang, Yuhua; Li, Xuying; Liu, Huayun; Zhou, Lianqing] Cent S Univ, Xiangya Sch Med, Affiliated Canc Hosp, Dept Nursing,Hunan Canc Hosp, Changsha 410013, Hunan, Peoples R China.;[Wu, Yanping; He, Pingping] Univ South China, Sch Nursing, Hengyang 421001, Hunan, Peoples R China.;[Yu, Juping] Univ South Wales, Fac Life Sci & Educ, Pontypridd CF37 1DL, M Glam, Wales.
通讯机构：
[Chen, Yongyi] Cent S Univ, Xiangya Sch Med, Affiliated Canc Hosp, Dept Nursing,Hunan Canc Hosp, Changsha 410013, Hunan, Peoples R China.;[He, Pingping] Univ South China, Sch Nursing, Hengyang 421001, Hunan, Peoples R China.
关键词：
Instrument;Scale;Psychometric properties;FSFI;Female sexual function;Chinese
摘要：
Purpose: This study aimed to examine the psychometric properties and performance of a Chinese version of the Female Sexual Function Index (FSFI) among a sample of Chinese women with cervical cancer. Methods: A cross-sectional survey design was used. The respondents included 215 women with cervical cancer in an oncology hospital in China. A translated Chinese version of the FSFI was used to investigate their sexual functioning. Psychometric testing included internal consistency reliability (Cronbach's alpha coefficient and item total correlations), test-retest reliability, construct validity (principal component analysis via oblique rotation and confirmatory factor analysis), and variability (floor and ceiling effects). Results: The mean score of the total scale was 20.65 +/- 4.77. The Cronbach values were .94 for the total scale,.72.90 for the domains. Test-retest correlation coefficients over 2-4 weeks were.84 (p < .05) for the total scale, .68.83 for the subscales. Item-total correlation coefficients ranged between .47 and .83 (p < .05). A five -factor model was identified via principal component analysis and established by confirmatory factor analysis, including desire/arousal, lubrication, orgasm, satisfaction, and pain. There was no evidence of floor or ceiling effects. Conclusions: With good psychometric properties similar to its original English version, this Chinese version of the FSFI is demonstrated to be a reliable and valid instrument that can be used to assess sexual functioning of women with cervical cancer in China. Future research is still needed to confirm its psychometric properties and performance among a large sample. (C) 2015 Elsevier Ltd. All rights reserved.
期刊：
Asian Nursing Research,2017年11(3):187-193 ISSN：1976-1317
通讯作者：
He, Guo-Ping
作者机构：
[Huang, Yan-Jin; He, Guo-Ping] Cent S Univ, Dept Community Nursing, Xiangya Nursing Sch, Changsha, Hunan, Peoples R China.;[Parry, Monica] Univ Toronto, Dept Nurse Practitioner Field Study, Lawrence S Bloomberg Fac Nursing, Toronto, ON, Canada.;[Zeng, Ying] Univ South China, Dept Int & Humanist Nursing, Sch Nursing, Hengyang, Peoples R China.;[Luo, Yan] Xi An Jiao Tong Univ, Dept Surg Nursing, Sch Nursing, Xian, Shaanxi, Peoples R China.;[Yang, Jing] Univ South China, Affiliated Hosp 2, Dept Nursing, Hengyang, Peoples R China.
通讯机构：
[He, Guo-Ping] Cent S Univ, Xiangya Nursing Sch, 172 Tongzipo Rd, Changsha 410013, Hunan, Peoples R China.
摘要：
Purpose: Early detection and management of coronary heart disease (CHD) are embedded into many community health service and primary care practices in western countries. The Framingham CHD risk score has been used to predict CHD and mortality for nearly 20 years, and it has predicted CHD event risk accurately in multiethnic populations. The aim of this study was to access the effect of a 6-month community-based intervention on CHD risk in individuals at high risk. Methods: A randomized controlled trial of individuals with a high 10-year CHD risk were recruited from two communities in China. Individuals in the intervention group (n = 53) received a 3-month group education and a 3-month coaching session. Physical examination and self-report questionnaires were used to collect both pre-and postintervention data on blood pressure, glucose, cholesterol, body mass index, smoking, depression, and health-related quality of life (HRQoL). Results: A total of 102 participants (85.0%) completed the 6-month study. Compared with the usual care group, the intervention group had a 5 mmHg greater reduction in systolic blood pressure (t = 2.01, p =.047), larger declines in glucose (t = -2.49, p =.015), cholesterol (t = -2.44, p =.017), body mass index (t = -2.58, p =.011), and depression (t = -2.05, p =.043), and better reports of HRQoL (t = 3.36, p =.001). No significant group differences in smoking behaviors were reported. Conclusion: A 6-month community-based intervention in a CHD high-risk population improved diseaserelated risk factors, depression, and HRQoL. Results provide preliminary evidence for primary prevention of cardiovascular disease risk in a community high-risk population. (C) 2017 Korean Society of Nursing Science, Published by Elsevier Korea LLC. This is an open access article under the CC BY-NC-ND license.
摘要：
Nasopharyngeal carcinoma (NPC) has a highly increased incidence rate (20/100,000) in Southern regions of China, while being rare in the rest of the world. NPC is a malignant type of cancer due to its high occurrence rate of metastasis; however, biomarkers for effective diagnosis and treatment are yet to be identified. Annexin A1 is a glucocorticoid-regulated member of a large superfamily of calcium and phospholipid-binding proteins and has been shown to have important roles in tumor development and progression, and was demonstrated to be a prognostic biomarker for head and neck cancer types. A previous study by our group showed that Annexin A1 was decreased in NPC tissue as compared with normal adjacent tissue. To investigate whether Annexin A1 is a potential biomarker for NPC, the present study assessed the effect of the Annexin A1 on the biological behavior (i.e., invasion and metastasis) of the highly metastatic NPC cell line 5-8F and the non-metastatic NPC cell line 6-10B. The expression levels of Annexin Al in the above two cell lines were determined by western blot analysis. Next, the recombinant plasmid pEGFP-C1-Annexin Al and the small interfering (si) RNA plasmid pRNAT-U6.1-Annexin Al were used and stably transfected into 5-8F and 6-10B cells, respectively. These established recombinant cell lines were then used to study the up- and downregulation of Annexin Al, respectively. The correlation of Annexin Al expression levels with the biological behavior of NPC cell lines was analyzed using a cell proliferation assay, flow cytometry, soft agar colony formation assay, as well as Transwell invasion and migration assays. The results demonstrated that upregulation of Annexin Al suppressed the proliferation, invasion and migration of NPC cells, while downregulation of Annexin Al promoted the proliferation, invasion and migration of NPC cells. These findings suggested that Annexin Al may be a potential biomarker for the development and prognosis of NPC, and its dysregulation may have an important role in its underlying pathogenesis.
作者机构：
[Zhao, L.] Cent S Univ, Xiangya Nursing Sch, Changsha, Hunan, Peoples R China.;[Zhao, L.] Univ South China, Sch Nursing, Heangyang, Peoples R China.;[Yan, J.] Xiangya Hosp 3, Dept Nursing, Changsha, Hunan, Peoples R China.;[Yang, G. -L.; Liu, Y.] Xiangya Hosp 3, Organ Transplantat Ctr, Tongzipo Rd 138, Changsha 410013, Hunan, Peoples R China.
通讯机构：
[Liu, Y.] Xiangya Hosp 3, Organ Transplantat Ctr, Tongzipo Rd 138, Changsha 410013, Hunan, Peoples R China.
摘要：
BACKGROUND: Adherence to follow-up is vital for the medical surveillance of the postoperative blood concentration, but relatively little research has examined it, and there is less study on relationships between adherence to follow-up and quality of life (QoL). We investigated the status of adherence to follow-up and QoL and associated factors among kidney transplantation recipients in China. METHODS: A cross-sectional study with the use of a Kidney Transplantation Recipient's Adherence to Follow-Up Questionnaire and a Quality of Life of Kidney Transplantation Recipients Questionnaire was conducted among 250 kidney transplantation recipients in Changsha, China, from January to March in 2015. RESULTS: The mean score for adherence to follow-up was 54.71 +/- 6.46. Time after transplantation was the only factor affecting adherence to follow-up scores (beta = -0.210; P < .05). The mean total score for QoL was 140.39 +/- 13.56; physical functioning, 23.72 +/- 3.33; psychologic functioning, 24.46 +/- 4.23; social functioning, 44.43 +/- 6.80; treatment, 24.81 +/- 2.97; and subjective satisfaction, 21.28 +/- 3.15. Multiple linear regression analysis showed that adherence to follow-up, economic level, job status, donor source, and original disease affected with QoL. CONCLUSIONS: Adherence to follow-up decreases with time after transplantation, and better compliance is associated with better QoL in all areas. Improvements in adherence to follow-up, income and reimbursement, psychologic guidance, and social support may increase QoL of kidney transplantat recipients.
摘要：
Recent studies have suggested that miR-590 may play critical roles in cardiovascular disease. This study was designed to determine the effects of miR-590 on lipoprotein lipase (LPL) expression and development of atherosclerosis in apolipoprotein E knockout (apoE(-/-)) mice and explore the potential mechanisms. En face analysis of the whole aorta revealed that miR-590 significantly decreased aortic atherosclerotic plaque size and lipid content in apoE (-/-) mice. Double immunofluorescence staining in cross-sections of the proximal aorta showed that miR-590 agomir reduced CD68 and LPL expression in macrophages in atherosclerotic lesions. MiR-590 agomir down-regulated LPL mRNA and protein expression as analyzed by RT-qPCR and western blotting analyses, respectively. Consistently, miR-590 decreased the expression of CD36 and scavenger receptor A1 (SRA1) mRNA and protein. High-performance liquid chromatography (HPLC) analysis confirmed that treatment with miR-590 agomir reduced lipid levels either in plasma orinabdominal cavity macrophages of apoE(-/-) mice. ELISA analysis showed that miR-590 agomir decreased plasma levels of proinflammatory cytokines, such as tumor necrosis factor-alpha (TNF-alpha), monocyte chemotactic protein-1 (MCP-1), interleukin-1 beta (IL-1 beta) and interleukin-6 (IL-6). In contrast, treatment with miR-590 antagomir prevented or reversed these effects. Taken together, these results reveal a novel mechanism of miR-590 effects, and may provide new insights into the development of strategies for attenuating lipid accumulation and pro-inflammatory cytokine secretion.
作者机构：
[Qu, Jia-Quan; Li, Cui; Yi, Hong; Li, Mao-Yu; Xiao, Zhi-Qiang; Zhang, Peng-Fei; Li, Xin-Hui; Zeng, Gu-Qing; Hu, Rong] Cent S Univ, Xiangya Hosp, Chinese Minist Hlth, Key Lab Canc Prote, Changsha, Hunan, Peoples R China.;[Zeng, Gu-Qing] Univ South China, Sch Nursing, Hengyang, Hunan, Peoples R China.;[Deng, Xingming] Emory Univ, Sch Med, Dept Radiat Oncol, Atlanta, GA 30322 USA.;[Deng, Xingming] Emory Univ, Winship Canc Inst, Atlanta, GA 30322 USA.
通讯机构：
[Deng, Xingming] Emory Univ, Sch Med, Dept Radiat Oncol, Atlanta, GA 30322 USA.
关键词：
Carcinogenesis;Selenium;Epithelial cells;Apoptosis;Protein expression;Biomarkers;Lung and intrathoracic tumors;Proteomics
摘要：
BACKGROUND: Our quantitative proteomic study showed that selenium-binding protein 1 (SELENBP1) was progressively decreased in human bronchial epithelial carcinogenic process. However, there is little information on expression and function of SELENBP1 during human lung squamous cell cancer (LSCC) carcinogenesis. METHODS: iTRAQ-tagging combined with 2D LC-MS/MS analysis was used to identify differentially expressed proteins in the human bronchial epithelial carcinogenic process. SELENBP1, member of selenoproteins family and progressively downregulated in this process, was selected to further study. Both Western blotting and immunohistochemistry were performed to detect SELENBP1 expression in independent sets of tissues of bronchial epithelial carcinogenesis, and ability of SELENBP1 for discriminating NBE (normal bronchial epithelium) from preneoplastic lesions from invasive LSCC was evaluated. The effects of SELENBP1 downregulation on the susceptibility of benzo(a)pyrene (B[a]P)-induced human bronchial epithelial cell transformation were determined. RESULTS: 102 differentially expressed proteins were identified by quantitative proteomics, and SELENBP1 was found and confirmed being progressively decreased in the human bronchial epithelial carcinogenic process. The sensitivity and specificity of SELENBP1 were 80% and 79% in discriminating NBE from preneoplastic lesions, 79% and 82% in discriminating NBE from invasive LSCC, and 77% and 71% in discriminating preneoplastic lesions from invasive LSCC, respectively. Furthermore, knockdown of SELENBP1 in immortalized human bronchial epithelial cell line 16HBE cells significantly increased the efficiency of B[a]P-induced cell transformation. CONCLUSIONS: The present data shows for the first time that decreased SELENBP1 is an early event in LSCC, increases B[a]P-induced human bronchial epithelial cell transformation, and might serve as a novel potential biomarker for early detection of LSCC.
