作者机构：
[贺荣芳; 赵强; 龚邵新; 张小丽] Department of Pathology, University of South China, Hengyang 421001, China;[王婧] School of Public Health, University of South China, Hengyang 421001, China;[谢黎明] Department of Medical Oncology, University of South China, Hengyang 421001, China
通讯机构：
[Xie, L.] D;Department of Medical Oncology, University of South China, China
作者机构：
[胡建安] College of Public Health, Central South University, Changsha 410078, China;[邱劲松; 龙颖; 黄云] College of Public Health, University of South China, Hengyang, 421001, China;[黄波] College of Public Health, Central South University, Changsha 410078, China, College of Public Health, University of South China, Hengyang, 421001, China
通讯机构：
[Hu, J.-A.] C;College of Public Health, Central South University, China
作者机构：
[姚碧云; 傅娟玲; 赵鹏; 袁准; 周宗灿] Department of Toxicology, Health Science Center, Peking University, Beijing 100191, China;Institute of Environmental Medicine and Radiation Hygiene, University of South China, Hengyang 421001, China;[应贤平; 仲伟鉴] Department of Toxicology, Shanghai Municipal Center for Disease Control and Prevention, Shanghai 200336, China;[张朝晖] Department of Toxicology, Health Science Center, Peking University, Beijing 100191, China, Institute of Environmental Medicine and Radiation Hygiene, University of South China, Hengyang 421001, China
通讯机构：
[Zhao, P.] D;Department of Toxicology, Health Science Center, Peking University, China
摘要：
OBJECTIVE: To investigate the nuclear proteomes in mitochondrial DNA (mtDNA)-depleted A549 cells (Rho0 cells) and their parental cells (Rho+ cells), and to learn more about the nuclear responses to mitochondrial dysfunction. METHODS: The nuclear proteomes of Rho and Rho + cells were characterized by two dimensional electrophoresis (2-DE) and SELDI-TOF ProteinChip technologies, the differentially expressed protein-spots were identified by MALDI-TOF mass spectrum (MS), the nucleophosmin and P53 expression were detected by Western blotting assay, and the mitochondrial membrane potential (MMP) was measured by the laser scanning confocal microscope. RESULTS: 2-DE results showed 11 protein-spots were down-regulated and 21 protein-spots were up-regulated in Rho0 cell nuclei. SELDI-TOF MS analysis with NP20 ProteinChips revealed 4 protein-peaks decreased in Rho0 cell nuclei. One down-regulated protein-spot was identified as eIF-6, and 4 up-regulated protein-spots were identified as nucleophosmin, SFRS1, SFRS3 and hnRNP G, respectively. The increased expression of nucleophosmin in Rho0 cells was verified by Western blotting. Based on the clues from proteomic analysis, P53 expression in Rho0 cells was higher than in Rho+ cells, and MMP was consistent in Rho+ and Rho0 cells. CONCLUSION: mtDNA-depletion induces nuclear proteome alteration. Rho0 cells can be used as a model to study the cross-talk between mitochondrion and nucleus.
作者机构：
[廖力夫; 聂长明; 肖锡林; 彭国文] School of Chemistry and Chemical Engineering, University South China, Hengyang 421001, Hunan, China;[杨胜园; 肖方竹] School of Public Health, University South China, Hengyang 421001, Hunan, China;[聂长明] Laboratory of Quantum Chemistry and Physics, University of Paul Sabatier(Toulouse-III), Toulouse 31077, France;[杨胜园] State Key Laboratory of Chemo-Biosensing and Chemometrics, Hunan University, Changsha 410082, Hunan, China
通讯机构：
School of Chemistry and Chemical Engineering, University South China, China
作者机构：
[聂长明; 肖方竹] School of Public Health, University South China, Hengyang 421001, Hunan, China;[杨胜园; 彭国文] School of Chemistry and Chemical Engineering, University South China, Hengyang 421001, Hunan, China;[聂长明] Laboratory of Quantum Chemistry and Physics, University of Paul Sabatier (Toulouse-III), Toulouse 31077, France;[杨胜园] State Key Laboratory of Chemo-Biosensing and Chemometrics, Hunan University, Changsha 410082, Hunan, China
通讯机构：
School of Chemistry and Chemical Engineering, University South China, China
作者机构：
[扶晓明] Department of Orthopaedics, the169 Hospital of Chinese PLA;[周艳] Department of Preventive Medicine, Hengyang Campus, Changsha College of Medicine;[李梓民] Department of Toxicology, Hunan Provincial Center of Disease Prevention and Control;[贺栋梁; 李东阳] Department o
