期刊论文

Zhao, P.

英文

中国药理学与毒理学杂志

1000-3002

2012

26

4

482-488

10.3867/j.issn.1000-3002.2012.04.003

The project supported by National Natural Science Foundation of China（30671781）;National Natural Science Foundation of China（81001253）； Fundamental Research Funds for Central Universities（BMU20090460）~~；

本校为其他机构

OBJECTIVE: To investigate the nuclear proteomes in mitochondrial DNA (mtDNA)-depleted A549 cells (Rho0 cells) and their parental cells (Rho+ cells), and to learn more about the nuclear responses to mitochondrial dysfunction. METHODS: The nuclear proteomes of Rho and Rho + cells were characterized by two dimensional electrophoresis (2-DE) and SELDI-TOF ProteinChip technologies, the differentially expressed protein-spots were identified by MALDI-TOF mass spectrum (MS), the nucleophosmin and P53 expression were detected by Western blotting assay, and the mitochondrial membrane potential (MMP) was...

目的比较线粒体DNA（mtDNA）缺失A549细胞（Rho^0细胞）与其母本细胞（Rho^0细胞）核蛋白表达谱，并探讨细胞核对线粒体功能缺陷的应答反应。方法二维凝胶电泳（2-DE）和表面增强激光法解吸电离．飞行时间（SELDI—TOF）蛋白芯片测定Rho^0细胞和Rho^＋细胞核蛋白表达谱，基质辅助激光解吸电离-飞行时问（MALDI-TOF）质谱结合数据库检索鉴定差异表达的蛋白点，Western印迹法测定核磷蛋白和P53表达，激光共聚焦显微镜测定线粒体膜电位。结果2-DE显示Rho^0细胞核中11个蛋白点表达下调，21个蛋白点表达上调。基于NP20蛋白质芯片的SELDI-TOF质谱分析发现4个蛋白质峰在Rho^0细胞核中明显下降。其中1个...