期刊论文

中文

光谱实验室

1004-8138

2011

28

2

782-786

10.3969/j.issn.1004-8138.2011.02.072

湖南省自然科学基金（03JJY3030） 湖南省科学技术基金（2008FJ3083）

本校为第一机构

利用反相高效液相色谱法建立了同时检测人尿样中的α-萘酚,β-萘酚,对硝基酚和间硝基酚的新方法.采用DiamonsilTMC18(150mm×4.6mm,5μm)色谱柱分离,以V(甲醇):V(乙酸铵缓冲液)=58:42为流动相,流速为0.75mL/min,柱温为35℃,于波长280nm处检测.当α-萘酚,β-萘酚,对硝基酚和间硝基酚的浓度分别在0.120--19.44,0.106--19.44,0.479--27.80μg/mL和0.439--27.80μg/mL范围内与峰面积呈良好线性关系,RSD分别为3.7%--4.3%,2.4%--3.6%,2.7%--4.9%和2.9%--3.6%(n=5).本法用于尿中4种物质的同时测定,回收率分别为98.8%,103.8%,97.5%和105.0%,结果满意

A novel method for the determination of α-naphthol,β-naphthol,p-nitrophenol and m-nitrophenol in human urine was established by RP-HPLC.The four objects were separated on DiamonsilTMC18（150mm×4.6mm,5μm） using methanol-ammonium acetate buffer solution（V∶V=58∶42） as mobile phase and the flow rate was 0.75mL/min.They were detected at 280nm when the column temperature was 35℃.The calibration curves of α-naphthol,β-naphthol,p-nitrophenol and m-nitrophenol showed good linearities in the ranges of 0.120—19.44,0.106—19.44,0.479—27.80μg...