作者机构：
[唐振平; 段毅; 刘迎九; 周帅] Hunan Province Key Laboratory of Pollution Control and Resources Reuse Technology, University of South China, Hengyang;421001, China;Hunan Key Laboratory of Rare Metal Minerals Exploitation and Geological Disposal of Wastes, Hengyang;[肖莎莎; 高媛媛] School of Civil Engineering, University of South China, Hengyang;[吴月月; 陈怡雯] Institute of Pathogenic Biology, Hengyang Medical College, University of South China, Hengyang
关键词：
石化废水;耐药菌(ARB);耐药基因(ARGs);分布;去除;影响因子
摘要：
抗生素耐药性污染已成为全球新兴环境问题之一.本研究选取某座石化废水处理厂,对耐药菌(ARB)和3种形态耐药基因(ARGs):细胞内耐药基因(iARGs) 、细胞外附着态耐药基因(aeARGs)和游离态耐药基因(feARGs)的分布特征与去除效能开展研究.结果表明,废水处理厂中检出四环素、磺胺和氨苄西林这3类ARB,其绝对浓度为8.45×10~2 ~ 2.38×10~5CFU·mL~(-1).厌氧处理可使这3类ARB绝对浓度下降0.04 lg ~ 0.21 lg;曝气和沉淀处理对ARB的影响因其类型而异;出水ARB绝对浓度高出进水水平0.12 lg ~ 0.63 lg.活性污泥中aeARGs和iARGs绝对丰度分别为1.96×10~7 ~ 3.02×10~(10) copies·g~(-1)和5.22×10~7 ~ 4.15×10~(10) copies·g~(-1);而废水中feARGs绝对丰度为5.90×10~8 ~ 1.01×10~(12) copies·L~(-1).厌氧处理可去除0.13 lg ~ 0.65 lg aeARGs和0.04 lg ~ 0.28 lg iARGs;曝气和沉淀处理对aeARGs和iARGs的去除效果受ARGs类型和形态影响;出水中feARGs绝对丰度较进水升高0.06 lg ~ 0.81 lg.冗余分析表明,ARB浓度与COD、Cl~-和总氮浓度显著正相关(P < 0.05); aeARGs丰度与COD和总氮浓度显著正相关(P < 0.05); iARGs和feARGs丰度均与重金属浓度显著正相关(P < 0.05).本研究证实了石化废水处理厂具有ARB和不同形态ARGs的富集风险,并为特种工业废水耐药性污染研究与防治提供理论基础.
作者机构：
[王雷; 李想; 刘灿; 沈婉莹; 让蔚清] Department of Preventive Medicine, School of Public Health, University of South China, Hengyang, 421001, China;[熊文婧] Department of Nephrology, Hengyang Hospital of Traditional Chinese Medicine, Hengyang, 421001, China
通讯机构：
[Rang, W.-Q.] D;Department of Preventive Medicine, China
摘要：
The cellular response to DNA damage is crucial for maintaining the integrity and stability of molecular structure. To maintain genome stability, DNA-damaged cells should be arrested so that mutations can be repaired before replication. Although several key components required for this arrest have been discovered, the majority of the pathways are still unclear. Through a number of assays, including cell viability, colony formation, and apotheosis assay, we found that AKR1B10 protected cells from UVC-induced DNA damage. Surprisingly, UVC-induced gamma H2AX foci and DNA double-strand breaks in the AKR1B10-overexpressing cells were similar to 4-5 folds lower than those in the control group. The expression levels of AKR1B10, p53, chk1, chk2, nuclear factor (NF)-kappa B, and p65 showed dynamic changes in response to UVC irradiation. Our results suggested that AKR1B10 is involved in the pathway of cell cycle checkpoint and NE-kappa B in DNA damage. Taken together, our results suggest that AKR1B10 is involved in the repair of the DNA double-strand break, which provides a new insight into the role of AKR1B10 in DNA damage repair and indicates a new trail in tumorigenesis and cancer drug resistance.
作者机构：
[钟光明; 谭水; 李忠玉; 余南燕; 王英姿; 周洲; 向文静; 陈超群; 李晓芳; 黄立军] Institute of Pathogenic Biology, Hunan Provincial Key Laboratory for Special Pathogens Prevention and Control, Medical College, University of South China, Hengyang, 421001, China
通讯机构：
[Zhou, Z.] I;Institute of Pathogenic Biology, China
作者机构：
[倪盼盼] Graduate School of Chengde Medical University, Chengde, 067000, China;[吴超] Department of Radiotherapy and Chemotherapy, Hebei Cangzhou Hospital of Integrated Traditional Chinese and Western Medicine, Cangzhou, 061000, China;[张静; 李会敏] Graduate School of North China University of Science and Technology, Tangshan, 063700, China;[牛芳兵] The Third Clinical School, Nanjing University of Chinese Medicine, Nanjing, 210046, China;[杨明秀] Hengyang Medical College, Nanhua University, Hengyang, 421000, China
通讯机构：
[Zhang, L.] D;Department of Pulmonory Disease, China
作者机构：
[Haiyan S.; Shuangyang T.; Hongrong W.; Yaqi L.; Shuang D.] Pathogenic Biology Institute, Hunan Provincial Key Laboratory for Special Pathogens Prevention and Control, University of South China, Hengyang, 421001, China;[Le L.; Yu H.] School of Public Health, University of South China, Hengyang, 421001, China;[Yuling X.] Hengyang Medicine School, University of South China, Hengyang, 421001, China
通讯机构：
[Wang, A.] H;Hengyang Medical College, China
关键词：
高血压;动脉硬化;肱-踝脉搏波传导速度;颈-股脉搏波传导速度;横断面调查
摘要：
目的抽样调查上海社区人群的血压情况,比较不同诊断标准下高血压患者、正常血压人群的动脉硬化程度。方法纳入上海地区3 815例居民。按不同诊断标准,将抽样人群分为正常血压人群(含用药后血压正常的高血压患者)、AHA诊断标准高血压患者(达到AHA诊断标准但未达到经典诊断标准)和经典诊断标准高血压患者,比较不同组间高血压相关指标、颈-股脉搏波传导速度(cf-PWV)和肱-踝脉搏波传导速度(ba-PWV)的差异。采用SPSS 13.0软件进行统计分析。结果与经典诊断标准诊断的高血压患者相比,AHA诊断标准诊断的高血压患者平均年龄更小(70.2±7.4 vs. 71.4±7.9岁,P<0.001),高血压病史比例更少(48.8%vs. 72.7%,P<0.001),体质量指数(24.1±3.5 vs. 24.7±3.9 kg/m^(2),P<0.001)、低密度脂蛋白(3.07±0.92 vs. 3.15±0.97 mmol/L,P=0.033)、cfPWV(8.7±2.7 vs. 9.8±3.0 m/s,P<0.001)和ba-PWV(1 647.7±610.1 vs. 1 797.2±729.7 cm/s,P<0.001)水平均更低。结论 AHA诊断标准诊断的高血压患者动脉硬化程度处于正常血压人群和经典诊断标准诊断的高血压患者之间。对这部分患者应积极控制血压,延缓动脉硬化的进展。
作者机构：
[陈飞; 熊强; 张震] National Center for Occupational Safety and Health, National Health Commission of the People's Republic of China, Beijing, 102308, China;[杨海兰; 杨胜园] School of Public Health, Hengyang Medical School, University of South China, Hengyang, 421001, China;[Yang H.] China Institute for Radiation Protection, Taiyuan, 030006, China
通讯机构：
[Chen, F.] N;National Center for Occupational Safety and Health, China
作者机构：
[李婷婷; 李水红; 丁楠; 丁伟艳; 朱翠明] Hunan Provincial Key Laboratory for Special Pathogens Prevention and Control, Institute of Pathogenic Biology, University of South China, Hengyang, 421001, China;[谭田平] Department of Clinical Laboratory, Affiliated Nanhua Hospital, University of South China, Hengyang, 421002, China;[黄立军] Hunan Provincial Graduate Training Innovation Base, South China University-Nanyue Biopharming Corporation, Ltd., Hengyang, 421900, China
通讯机构：
[Zhu, C.] H;Hunan Provincial Key Laboratory for Special Pathogens Prevention and Control, China
