期刊论文

Exenatide promotes cholesterol efflux in pancreatic tissue of obese diabetic rats

中文

南方医科大学学报

1673-4254

2021

41

03

370-375

10.12122/j.issn.1673-4254.2021.03.08

81900488:国家自然科学基金 2019JJ50549:湖南省自然科学基金 2018SK51605:湖南省临床医疗创新引导计划 HKF2019Y47N207:衡阳市指导性科技项目

本校为第一机构

目的 利用肥胖糖尿病大鼠模型研究艾塞那肽对胰腺组织ABCA1表达及胆固醇代谢的影响.方法 SD雄性大鼠48只,选取部分大鼠通过高脂饮食和STZ药物诱导建立肥胖合并2型糖尿病大鼠模型,所有大鼠分为4组:正常大鼠注射生理盐水组(A组,12只),正常大鼠注射艾塞那肽组(B组,12只),肥胖糖尿病大鼠注射生理盐水组(C组,9只),糖尿病模型大鼠艾塞那肽组(D组,9只),10周后通过real-time PCR、Western blot、油红"O"染色、组织胆固醇含量测定、HE染色等检测艾塞那肽对大鼠胰腺组织ABCA1表达、胆固醇代谢、胰岛功能的影响.结果 通过real-time PCR、Western blot等发现较之SD大鼠,肥胖糖尿病大鼠胰腺组织ABCA1表达降低...

Objective To study the effect of exenatide on the expression of ABCA1 and cholesterol metabolism in the pancreas of obese diabetic rats. Methods Twenty- four normal male SD rats and 18 obese diabetic rats (induced by high-fat feeding and STZ injection) were both divided equally into 2 groups for injections of saline or exenatide. After treatment for a week, the expression of ABCA1, cholesterol metabolism, and islet function of the rats were examined using real-time PCR, Western blotting, oil red O staining, cholesterol content determination, and HE staining. Results The expressions of ABCA1 at...