期刊论文

Relationship between complete Freund’s adjuvant-induced chronic pain-induced negative emotions and morphological changes of hippocampal microglia

中文

解剖学报

0529-1356

2021

52

03

352-357

10.16098/j.issn.0529-1356.2021.03.004

湖南省自然科学基金省市联合项目（2018JJ4034）;湖南省自然科学基金青年项目（2018JJ3464）； 湖南省教育厅优秀青年项目（19B576）；

本校为第一机构

目的探讨完全弗氏佐剂(CFA)所致慢性炎性痛小鼠模型情绪及海马小胶质细胞形态的改变。方法32只ICR雄性小鼠随机分成生理盐水对照组(NS)和完全弗氏佐剂组(CFA) 。右后爪足底注射CFA建立小鼠慢性炎性痛模型。采用Von Frey纤维针测量小鼠右足底注射CFA后机械痛阈的变化;采用旷场实验检测小鼠活动度和焦虑样行为;采用糖水偏好和被迫游泳实验检测小鼠抑郁样行为;采用免疫组织化学法检测小鼠海马小胶质细胞标记物离子钙接头蛋白抗原(IBA-1)的表达;采用Sholl分析方法分析海马齿状回区小胶质细胞形态变化。结果小鼠痛阈评分结果显示,与NS组相比,CFA组小鼠机械痛阈明显下降(P

Objective To investigate the alteration of mood and hippocampal microglia morphology in a mouse model of chronic inflammatory pain induced by complete Freund’s adjuvant (CFA). Methods Thirty-two male ICR mice were randomly divided into two groups,including normal saline control group (NS) and CFA model group (CFA). The pain model was established by right hindpaw intraplantar CFA injection. The change of mechanical pain threshold after CFA injection was measured by von Frey fiber needle,the locomotor activity and anxiety-like behavior were dete...