期刊论文

Effects of Daxx on γ-ray induced apoptosis of HeLa cells

Le, L.

中文

辐射研究与辐射工艺学报

1000-3436

2021

39

5

55-60

10.11889/j.1000-3436.2021.rrj.39.050302

基金资助：湖南省教育厅青年项目(18B269)、湖南省卫生健康委科研重点项目(202105012137)、湖南省大学生创新创业训练 计划项目(湘教通[2020]191号No.2683)、衡阳市科技计划项目(202002042122)、国家自然科学基金项目(81402169)、湖南省 自然科学基金项目(2018JJ3452、202150014)和特殊病原体防控湖南省重点实验室资助项目（ 湘科计字 [2014]5号 ）资助 第一作者：唐双阳，女，1979 年9月出生，2008 年于南华大学获硕士学位 通信作者：李乐，教授，E-mail: usclile@126.com 收稿日期：初稿 2021-01-28；修回 2021-06-23 Supported by Educational Committee Foundation of Hunan Province (18B269), Health Commission Foundation of Hunan Province (202105012137), College Students' Innovative Entrepreneurial T raining Project of Hunan Province (2020-191-2683), Municipal Science Project of Hengyang City in Hunan Province (202002042122), National Natural Science Foundation of China (81402169), Natural Science Foundation of Hunan Province (2018JJ3452, 202150014), and Foundation of Hunan Provincial Key Laboratory for Special Pathogens Prevention and Control (2014-5) First author: T ANG Shuangyang (female) was born in September 1979, and obtained her master’ s degree from University of South Chinain2008 Corresponding author: LI Le, professor , E-mail: usclile@126.com Received28January2021;accepted23June2021

本校为第一机构

研究过表达死亡结构域相关蛋白(Daxx)对经γ射线照射过的细胞凋亡的影响,探讨Daxx与辐射诱导细胞凋亡之间的关系及其机制。通过转染将宫颈癌HeLa细胞分为正常对照组、空质粒转染阴性组与Daxx转染组,利用生物细胞辐照仪经γ射线照射至不同剂量;Western blot检测细胞中Daxx的表达;MTT检测细胞的增殖情况;ELISA检测细胞中Caspase-8的相对活性;流式细胞术检测细胞凋亡情况。结果发现:随着照射剂量增加,细胞数量逐渐减少,且其中变形细胞越来越多;Daxx表达水平随着照射剂量增加而升高; Daxx转染的辐照细胞组的细胞增殖抑制率、A_(405)值以及细胞凋亡率均明显高于未辐照细胞组,差异均有统计学意义(p

We studied the effects of overexpression of deathdomainassociated protein (Daxx) on cell apoptosis under γ -ray radiation. Further, we explored the relationship between Daxx and radiation-induced apoptosis and investigated the mechanism behind such apoptosis. Through transfection, cervicalcancer HeLa cells were divided into a control group, an empty plasmid-transfected negative group, and a Daxx-transfected group; the HeLa cells were irradiated with different doses of γ-rays using a cell irradiator. The expression of Daxx protein in cells was...