摘要:
Metal complexes, particularly copper(II) complexes, are often used as anticancer drugs due to their ability to generate reactive oxygen species (ROS) in cells. Four copper(II) complexes have been designed based on ligands for triplet pyridine derivatives (complexes 1-4), and their structures have been determined using X-ray single crystal analysis. The interactions of these complexes with calf thymus DNA (CT-DNA) have been investigated using various techniques, including UV-vis absorption, viscosity measurements, and circular dichroism spectroscopy. The results indicate that complexes 1-4 strongly interact with DNA through partial intercalations. Further investigation using agarose gel electrophoresis shows that all four complexes can cleave pBR322 DNA in the presence of ascorbic acid as a reducing agent, and the DNA cleavage mechanism is through the generation of singlet oxygen ((1)O(2)). In vitro anticancer activities of these complexes have been evaluated using A549, MDA-MB-231, HeLa, and HepG2 cells. The calculated IC(50) values indicate significant efficacy against cancer cells. Additionally, AO/EB staining assays reveal that these complexes induce cell apoptosis in HeLa cell line.
摘要:
Research on porphyrin-based photosensitizing drugs is becoming increasingly popular. They possess unique diagnostic capabilities and therapeutic effects that have gained wide recognition in oncology drug development. In recent years, the rapid growth of nanotechnology has brought great hope for nanopharmaceutical formula-tions. By combining porphyrins with various nanomaterials, people have improved the properties of porphyrin compounds, making drug delivery easier. Porphyrin-based nanoparticles can enhance the effect of photodynamic therapy for cancer treatment, providing opportunities for achieving complex targeting strategies and versatility with promising applications in drug carriers, tumor imaging, and treatment. This paper reviews recent porphyrin nanodrugs, including inorganic-organic hybrid nanoparticles, nanomicelles, self-assembled nanoparticles, and combination therapeutic nanodrugs, and their actions and effects on cancer cells when performing photodynamic therapy. It also discusses the drawbacks as well as the prospects for development.
作者机构:
[Tan, Huaxin; Hu, Lidan; Wei, Xiaojie; Li, Yongzhen; Ma, Jiaying] Univ South China, Hunan Prov Cooperat Innovat Ctr Mol Target New Dru, Sch Basic Med,Key Lab Ecol Environm & Crit Human D, Dept Biochem & Mol Biol,Dept Educ, Hengyang 421001, Peoples R China.;[Jiang, Suhua; Wang, Peiyuan; Ma, Jiaying] Chinese Acad Sci, Fujian Inst Res Struct Matter, Key Lab Design & Assembly Funct Nanostruct, Fuzhou 350002, Peoples R China.;[Jiang, Suhua; Zhu, Fukai] Minnan Normal Univ, Collaborat Innovat Ctr Mushroom Hlth Ind, Zhangzhou 363000, Fujian, Peoples R China.;[Ji, Xiaoxuan] Xiamen Univ, Sch Pharmaceut Sci, State Key Lab Cellular Stress Biol, Xiamen 361102, Peoples R China.;[Wang, Peiyuan] Chinese Acad Sci, Xiamen Inst Rare Earth Mat, Dept Translat Med, Xiamen 361021, Peoples R China.
通讯机构:
[Huaxin Tan] D;[Peiyuan Wang] K;Department of Biochemistry and Molecular Biology, the Key Laboratory of Ecological Environment and Critical Human Diseases Prevention of Hunan Province Department of Education, Hunan Province Cooperative Innovation Center for Molecular Target New Drug Study, School of Basic Medicine, University of South China, Hengyang 421001, PR China<&wdkj&>Key Laboratory of Design and Assembly of Functional Nanostructures, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, Fuzhou 350002, PR China<&wdkj&>Department of Translational Medicine, Xiamen Institute of Rare Earth Materials, Chinese Academy of Sciences, Xiamen 361021, PR China
摘要:
Green tea is one of people's favorite drinks. However, pesticide residues in green tea can cause harm to the human body, and therefore, detection of pesticide residues in green tea is very important. In recent years, the detection of pesticide residues in tea has become a research hotspot. In this paper, a gas chromatography-mass spectrometry/mass spectrometry (GC-MS/MS) detection method of tolfenpyrad pesticide residues in green tea was established by using acetonitrile extractant, dispersive solid-phase extraction purification, temperature programming and application retention time lock with the database. After the sample was extracted with acetonitrile, then the sample was purified by QuEChERS extraction purification tube, afterward isomer B was used as the internal standard for the determination by multiple reaction monitoring mode (MRM) of GC-MS/MS. The results indicated that the experimental data accorded with the criterion on quality control of laboratoris(chemical testing of food), and the requirements of recovery, calibration curve, precision.This method was used to detect tolfenpyrad residues in actual green tea samples in multiple batches, and the satisfactory results were obtained.
期刊:
Separation Science and Technology,2023年58(10):1833-1850 ISSN:0149-6395
通讯作者:
Nie, CM
作者机构:
[Liao, Li-Fu; Nie, Chang-Ming; Ma, Ming-Jie; Xiao, Chun-Mei; Nie, CM; Xiao, Xi-Lin] Univ South China, Sch Chem & Chem Engn, Hengyang 421001, Peoples R China.;[Liao, Li-Fu; Nie, Chang-Ming; Ma, Ming-Jie; Xiao, Chun-Mei; Xiao, Xi-Lin] Key Lab Hunan Prov Design & Applicat Nat Actinide, Hengyang, Peoples R China.
通讯机构:
[Nie, CM ] U;Univ South China, Sch Chem & Chem Engn, Hengyang 421001, Peoples R China.
关键词:
Uranyl-MMQBQ;chiral fungicides;enantiomeric separation;R/S-metalaxyls and R/S-benalaxyls;density functional theory (DFT)
摘要:
Chiral fungicides R/S-metalaxyls(R/S-MTLs) and R/S-benalaxyls(R/S-BNLs) have different biotoxicity. It is very important to unravel molecular recognition and enantiomeric separation of R/S-MTLs or R/S-BNLs. In this paper, we designed a novel asymmetric ligand: 5-methoxy-2-(4-methoxy-6”-(quinazolin-2-yl)-[2,2”−bipyridin]-6-yl)quinazoline(MMQBQ), further constructed a new asymmetric receptor Uranyl-MMQBQ by coordination of MMQBQ with uranyl. The complexation and separation of receptor Uranyl-MMQBQ with guests R/S-MTLs or R/S-BNLs were systematically studied using density functional theory (DFT) method. The respects of ESP, MBO, RFC, LOL, EDDM, EST-NOCV, QTAIM, FMOs, IGMH, and Gibbs free energy changes were calculated and analyzed. The results indicated that the MMQBQ could form a stable receptor Uranyl-MMQBQ with uranyl, which could selectively recognize and separate chiral R/S-MTLs or R/S-BNLs by receptor’s U coordinating to carbonyl oxygens of guests. For R/S-MTLs, separation factors(SFS/R) of Uranyl-MMQBQ were more than 16 in water, chlorobenzene, toluene, carbon tetrachloride and cyclohexane, enantioselectivity coefficients(ESCs) ranged of 99.92%-94.24% in the above four solvents. While for R/S-BNLs, ESCs of Uranyl-MMQBQ were over 99% and SFS/R values were a range of 533.97–2839.36 in the above organic solvents. The findings provide valuable information for design of novel uranyl ligand and its complexes, meanwhile supply useful guidance for experiments on separation of other chiral fungicides in environmental protection.
期刊:
Asian Journal of Organic Chemistry,2023年12(2):e202200658- ISSN:2193-5807
通讯作者:
He, W.-M.
作者机构:
[Lu, Yu-Han; Tan, Hong-Yu; He, Wei-Min; Song, Hai-Yang; Ji, Hong-Tao; Chen, Jin-Yang; Zhang, Zhuo-Tao] Univ South China, Sch Chem & Chem Engn, Hengyang 421001, Peoples R China.;[Yang, Tian-Bao] Nanjing Forestry Univ, Natl Engn Res Ctr Low Carbon Proc & Utilizat Fores, Nanjing 210037, Peoples R China.
通讯机构:
[Prof. Wei-Min He; Prof. Wei-Min He Prof. Wei-Min He Prof. Wei-Min He] S;School of Chemistry and Chemical Engineering, University of South China, Hengyang, 421001 P. R. China
关键词:
electron donor-acceptor complex;dual role;photocatalysis;quinoxalin-2(1H)-ones;visible-light
摘要:
Various 3‐alkylated quinoxalin‐2(1H)‐ones were synthesized through electron donor‐acceptor complex enabled alkylation of quinoxalin‐2(1H)‐ones with N‐hydroxyphthalimide esters (alkyl source) and Na2S (catalytic electron donor) under external photocatalyst‐, oxidant‐ and additive‐free conditions. Abstract We developed a novel and efficient method for the synthesis of various 3‐alkylated quinoxalin‐2(1H)‐ones (32 examples, 70%–96% yields) through electron donor‐acceptor complex enabled alkylation of quinoxalin‐2(1H)‐ones with N‐ hydroxyphthalimide esters as the alkyl source and Na2S as the catalytic electron donor under external photocatalyst‐, oxidant‐ and additive‐free conditions.
期刊:
Desalination and Water Treatment,2023年293:150-159 ISSN:1944-3994
通讯作者:
Wang, Ruibin;Wang, GP;Chen, N
作者机构:
[Wang, Ruibin; Lu, Yunfan; Wang, Guoping] Univ South China, Sch Chem & Chem Engn, Hengyang 421001, Peoples R China.;[Chen, Nian] Univ South China, Affiliated Hosp 1, Hengyang Med Sch, Dept Med Cosmet, Hengyang 421001, Peoples R China.;[Wang, RB; Wang, Guoping] Univ South China, Sch Chem & Chem Engn, Hengyang 421001, Peoples R China.;[Chen, Nian] Univ South China, Affiliated Hosp 1, Hengyang Med Sch, Dept Med Cosmet, Hengyang 421001, Peoples R China.
通讯机构:
[Wang, RB; Wang, GP ; Chen, N ] ;Univ South China, Sch Chem & Chem Engn, Hengyang 421001, Peoples R China.;Univ South China, Affiliated Hosp 1, Hengyang Med Sch, Dept Med Cosmet, Hengyang 421001, Peoples R China.
作者机构:
[Yang, Qinglai; He, Yuxuan] Univ South China, Hengyang Med Sch, Dept Biochem & Mol Biol, Hengyang 421001, Peoples R China.;[Tang, Li; Yang, Qinglai; Li, Na; Tan, Xiaofeng; He, Yuxuan; Yang, Sha] Univ South China, Canc Res Inst, Hunan Prov Key Lab Tumor Cellular & Mol Pathol, Hengyang Med Sch,Ctr Mol Imaging Probe, Hengyang 421001, Peoples R China.;[Tang, Li] Hainan Normal Univ, Coll Chem & Chem Engn, Key Lab Trop Med Plant Chem, Minist Educ, Haikou 571158, Peoples R China.
通讯机构:
[Qinglai Yang] D;Department of Biochemistry and Molecular Biology, Hengyang Medica School, University of South China, Hengyang 421001, China<&wdkj&>Center for Molecular Imaging Probe, Hunan Province Key Laboratory of Tumor Cellular and Molecular Pathology, Cancer Research Institute, Hengyang Medical School, University of South China, Hengyang 421001, China<&wdkj&>Author to whom correspondence should be addressed.
摘要:
In recent years, pathogenic infections have been a growing health threat due to the proliferation of drug-resistant bacteria, so photothermal therapy (PTT) has gained considerable interest in biological and medical fields, owing to its noninvasive and highly effective properties. However, it is hard to achieve selective bacteria targeting while generating a large amount of heat at infected sites. Cationic electrostatic interaction is considered to be a common antimicrobial strategy. Herein, an organic molecule named RT-MN was synthesized with four positively charged quaternary ammonium salts that can bind to negatively charged bacteria. Under near-infrared 808 nm laser irradiation, RT-MN could be efficiently converted into a large amount of heat to eradicate bacteria. In addition, its good water solubility and biological safety proved that RT-MN has excellent biological application prospects. Overall, four such positively charged photosensitizer RT-MN, as a non-antibiotic treatment for resistant bacteria, could be promising for the exploration of highly effective antibacterial agents.
摘要:
Heme proteins play various important roles in a variety of physiological and pathological processes. Sur-factant assemblies have drawn great attention in fabricating fluorescent sensors to detect and identify proteins. In this study, an acetylpyrene fluorophore containing imidazole HP-1 was synthesized, and it could be well modulated by an anionic surfactant sodium dodecyl sulfate (SDS). The selected ensemble based on HP-1/SDS assemblies exhibited selective fluorescence sensing performance towards the heme proteins, including neuroglobin (Ngb), myoglobin (Mb) and cytochrome c (Cyt c ). Besides, phospholipid DMPC vesicles as membrane models were particularly explored the association process between the heme protein Mb and membrane. The present work showed that Mb induced the lysis of DMPC lipo-somes visualized by transmission electron microscopy and optical microscope.(c) 2023 Published by Elsevier B.V. on behalf of Chinese Chemical Society and Institute of Materia Medica, Chinese Academy of Medical Sciences.
期刊:
Polymers for Advanced Technologies,2023年34(5):1515-1528 ISSN:1042-7147
通讯作者:
Ni Tan<&wdkj&>Ni Tan Ni Tan Ni Tan
作者机构:
[Wang, Duoduo; Zeng, Chensi; Ye, Qiaorong; Bao, Yuqi; Tan, Ni; Tan, Yaxin; Liu, Lijie] Univ South China, Sch Chem & Chem Engn, Hengyang, Peoples R China.;[Tan, Ni] Univ South China, Sch Chem & Chem Engn, Hengyang 421001, Peoples R China.
通讯机构:
[Ni Tan; Ni Tan Ni Tan Ni Tan] S;School of Chemistry and Chemical Engineering, University of South China, Hengyang, China
关键词:
drug delivery system;pH;GSH responsiveness;polyethylene glycol;Sorafenib;stealth imprinted material
摘要:
In order to improve the solubility and the long-cycle performance of hydrophobic drug sorafenib (SOR), and to obtain an ideal smart drug delivery system for the effective treatment of liver cancer, S-SMIP, a pH/GSH double-sensitive sorafenib stealth imprinting material, was successfully prepared by UV-initiated surface imprinted polymerization on the basis of polyethylene glycol 2000 (PEG(2000)) acted as "gate keeper " modified magnetic substrate. The results showed that S-SMIP had a good adsorption capacity of 260.0 mg g(-1) and an excellent selectivity factor alpha 2.33. S-SMIP's kinetic adsorption data accorded well with the pseudo-second-order model and its mechanism was controlled mainly by chemical adsorption. The drug release experiments in vitro indicated S-SMIP could not only greatly improve the solubility of SOR, but also had the pH/GSH dual-sensitive response performance. Compared with the non-stealth imprinted material SMIP, S-SMIP still had a better long-cycle effect on the release of SOR, increasing to160 h from 130 h. So, the target material S-SMIP could greatly prolong the half-life of SOR, which provided a good idea for the development of a smart drug delivery system.
作者:
Ren, Jieyu;Zeng, Qun;Wu, Hongmei;Liu, Xuewen;Guida, Maria C.;...
期刊:
JOURNAL OF CELLULAR PHYSIOLOGY,2023年238(3):647-658 ISSN:0021-9541
通讯作者:
Min Tang<&wdkj&>Min Tang Min Tang Min Tang
作者机构:
[Zeng, Qun; Li, Junjie; Wu, Hongmei; Liu, Xuewen; Tang, Min; Ren, Jieyu; Zhai, Yiyuan] Univ South China, Coll Hengyang Med, Dept Biochem & Mol Biol, Hengyang, Peoples R China.;[Ocorr, Karen; Guida, Maria C.; Bodmer, Rolf] Sanford Burnham Prebys Med Discovery Inst, Dev Aging & Regenerat Program, La Jolla, CA USA.;[Huang, Wen] Cent South Univ, Ctr Med Genet, Sch Life Sci, Changsha, Hunan, Peoples R China.;[Tang, Min] Univ South China, Changsheng West Rd 28,, Hengyang 421001, Peoples R China.
通讯机构:
[Min Tang; Min Tang Min Tang Min Tang] D;Department of Biochemistry and Molecular Biology, College of Hengyang Medical, University of South China, Hengyang, China
摘要:
Cardiomyopathy is a common disease of cardiac muscle that negatively affects cardiac function. HDAC3 commonly functions as corepressor by removing acetyl moieties from histone tails. However, a deacetylase-independent role of HDAC3 has also been described. Cardiac deletion of HDAC3 causes reduced cardiac contractility accompanied by lipid accumulation, but the molecular function of HDAC3 in cardiomyopathy remains unknown. We have used powerful genetic tools in Drosophila to investigate the enzymatic and nonenzymatic roles of HDAC3 in cardiomyopathy. Using the Drosophila heart model, we showed that cardiac-specific HDAC3 knockdown (KD) leads to prolonged systoles and reduced cardiac contractility. Immunohistochemistry revealed structural abnormalities characterized by myofiber disruption in HDAC3 KD hearts. Cardiac-specific HDAC3 KD showed increased levels of whole-body triglycerides and increased fibrosis. The introduction of deacetylase-dead HDAC3 mutant in HDAC3 KD background showed comparable results with wild-type HDAC3 in aspects of contractility and Pericardin deposition. However, deacetylase-dead HDAC3 mutants failed to improve triglyceride accumulation. Our data indicate that HDAC3 plays a deacetylase-independent role in maintaining cardiac contractility and preventing Pericardin deposition as well as a deacetylase-dependent role to maintain triglyceride homeostasis.
通讯机构:
[Xinhua Xu; Wei-Min He] A;Authors to whom correspondence should be addressed.<&wdkj&>School of Chemistry and Chemical Engineering, University of South China, Hengyang 421001, China<&wdkj&>Authors to whom correspondence should be addressed.<&wdkj&>College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, China
关键词:
green chemistry;paired electrolysis;diaryl diselenides;aryl selenocyanates;cyanation
摘要:
The first example of paired electrolysis-enabled cyanation of diaryl diselenides, with KSCN as the green cyanating agent, has been developed. A broad range of aryl selenocyanates can be efficiently synthesized under chemical-oxidant- and additive-free, energy-saving and mild conditions.
摘要:
A core transcription regulatory circuitry (CRC) is an interconnected self-regulatory circuitry that is formed by a group of core transcription factors (TFs). These core TFs collectively regulate gene expression by binding not only to their own super enhancers (SEs) but also to the SEs of one another. For most human tissue/cell types, a global view of CRCs and core TFs has not been generated. Here, we identified numerous CRCs using two identification methods and detailed the landscape of the CRCs driven by SEs in large cell/tissue samples. The comprehensive biological analyses, including sequence conservation, CRC activity and genome binding affinity were conducted for common TFs, moderate TFs, and specific TFs, which exhibit different biological features. The local module located from the common CRC network highlighted the essential functions and prognostic performance. The tissue-specific CRC network was highly related to cell identity. Core TFs in tissue-specific CRC networks exhibited disease markers, andhad regulatory potential for cancer immunotherapy. Moreover, a user-friendly resource named CRCdb (http://www.licpathway.net/crcdb/index.html) was developed, which contained the detailed information of CRCs and core TFs used in this study, as well as other interesting results, such as the most representative CRC, frequency of TFs, and indegree/outdegree of TFs.
作者机构:
[Liu, Jing-Jing; Baek, Kitae; Abeysinghe, Shakya; Kim, Hye-Bin; Kim, Jong-Gook] Jeonbuk Natl Univ, Dept Environm & Energy, 567 Baekje Daero, Jeonju Si, Jeollabukdo, South Korea.;[Liu, Jing-Jing; Baek, Kitae; Abeysinghe, Shakya; Kim, Hye-Bin; Kim, Jong-Gook] Jeonbuk Natl Univ, Soil Environm Res Ctr, 567 Baekje Daero, Jeonju Si, Jeollabukdo, South Korea.;[Liu, Jing-Jing; Lin, Ying-Wu] Univ South China, Sch Chem & Chem Engn, Hengyang 421001, Peoples R China.
通讯机构:
[Kitae Baek] D;Department of Environment & Energy and Soil Environment Research Center, Jeonbuk National University, 567 Baekje-daero, Deokjin-gu, Jeonju-si, Jeollabukdo, Republic of Korea
关键词:
Adsorption;Enzyme;Horseradish peroxidase;Hydrogen peroxide;Oxidation;Supporting material
作者机构:
[He, Wei-Min; Ouyang, Wen-Tao] Univ South China, Sch Chem & Chem Engn, Hengyang 421001, Peoples R China.;[Xiao, Fang] Cent South Univ, Xiangya Sch Publ Hlth, Changsha 410078, Hunan, Peoples R China.;[Ou, Li-Juan] Hunan Inst Technol, Sch Mat Sci & Engn, Hengyang, Peoples R China.
通讯机构:
[Li-Juan Ou; Wei-Min He] S;School of Materials Science and Engineering, Hunan Institute of Technology, Hengyang 421002, China<&wdkj&>School of Chemistry and Chemical Engineering, University of South China, Hengyang 421001, China
关键词:
Green chemistry;Hydrogenation;Oxygenation;Photocatalysis;Water
摘要:
Bis(diphenyl phosphate) imine(Htpop) was synthesized as an adsorbent for the effective removal of U(VI) from aqueous solutions using triphenyl phosphate(TPP) as the starting material. Htpop possesses notable polarity, characterized by the presence of -P--O(phosphoryl) and -N-H(imino) functional groups, along with other bulky substituents. It has the capability to form chelates with metal ions, thus enabling the removal of radionuclide U (VI) from aqueous solution. The overall adsorption experiment shows that both phosphoryl and imino groups interact with uranyl ions, and the removal rate reaches 99.53 % at pH 6.0. The adsorption process conforms to the Pseudo-second-order kinetic model and Langmuir isotherm model.