作者机构：
[Zhang Zhi-Wei; Cao Jian-Guo; Li Yan-Lan; Luo Zhao-Yang; He Xiu-Sheng] Univ So China, Coll Med, Canc Res Inst, Hengyang 421001, Peoples R China.;[Zhang Zhi-Wei; Li Yan-Lan; Luo Zhao-Yang; He Xiu-Sheng] Univ So China, Coll Med, Dept Pathol, Hengyang 421001, Peoples R China.;[Zhang Zhi-Wei; Li Yan-Lan; Luo Zhao-Yang; He Xiu-Sheng] Univ Key Lab Canc Cellular & Mol Pathol Hunan Pro, Hengyang 421001, Peoples R China.;[Cao Jian-Guo] Hunan Normal Univ, Coll Med, Dept Pharmacol, Changsha 410013, Hunan, Peoples R China.
通讯机构：
[Zhang Zhi-Wei] U;Univ So China, Coll Med, Canc Res Inst, Hengyang 421001, Peoples R China.
作者机构：
[Wang, Yuequn; Wan, Yongqi; Ye, Xiangli; Yuan, Wuzhou; Deng, Yun; Wang, Zequn; Zhou, Junmei; Mo, Xiaoyan; Li, Yongqing; Fan, Xiongwei; Yan, Yan; Luo, Na; Wu, Xiushan] Hunan Normal Univ, Coll Life Sci, Ctr Heart Dev, Changsha 410081, Hunan, Peoples R China.;[Zhou, Junmei] Univ S China, Coll Life Sci, Hengyang 421001, Peoples R China.
通讯机构：
[Deng, Yun] H;Hunan Normal Univ, Coll Life Sci, Ctr Heart Dev, Changsha 410081, Hunan, Peoples R China.
关键词：
NFAT;p21;Zinc finger protein;ZNF424
摘要：
Zinc finger-containing transcription factors are the largest single family of transcriptional regulators in mammals, which play an essential role in cell differentiation, cell proliferation, apoptosis, and neoplastic transformation. Here we have cloned a novel KRAB-related zinc finger gene, ZNF424, encoding a protein of 555aa. ZNF424 gene consisted of 4 exons and 3 in-trons, and mapped to chromosome 19p13.3. ZNF424 gene was ubiquitously expressed in human embryo tissues by Northern blot analysis. ZNF424 is conserved across species in evolution. Using a GFP-labeled ZNF424 protein, we demonstrate that ZNF424 localizes mostly in the nucleus. Transcriptional activity assays shows ZNF424 suppresses transcriptional activity of L8G5-luciferase. Overexpression of ZNF424 in HEK-293 cells inhibited the transcriptional activity of NFAT and p21, which may be silenced by siRNA. The results suggest that ZNF424 protein may act as a transcriptional repressor that suppresses NFAT and p21 pathway to mediate cellular functions.
期刊：
Journal of Neuroimaging,2010年20(3):234-239 ISSN：1051-2284
通讯作者：
Wong, Ka Sing
作者机构：
[You, Yong] Nanhua Univ, Affiliated Hosp 1, Dept Neurol, Hengyang, Hunan, Peoples R China.;[Lau, Alex; Mok, Vincent; Leung, Thomas; Leung, Howan; Chen, Xiangyan; Hao, Qing; Wong, Ka Sing] Chinese Univ Hong Kong, Dept Med & Therapeut, Shatin, Hong Kong, Peoples R China.;[Lau, Alex; Mok, Vincent; Leung, Thomas; Leung, Howan; Chen, Xiangyan; Hao, Qing; Wong, Ka Sing] Chinese Univ Hong Kong, Dept Diagnost Radiol & Organ Imaging, Shatin, Hong Kong, Peoples R China.;[Wong, Ka Sing] Chinese Univ Hong Kong, Prince Wales Hosp, Dept Med & Therapeut, Shatin, Hong Kong, Peoples R China.
通讯机构：
[Wong, Ka Sing] C;Chinese Univ Hong Kong, Prince Wales Hosp, Dept Med & Therapeut, Shatin, Hong Kong, Peoples R China.
摘要：
Using the first-principles study, we calculate the electronic band structure of metallic carbon nanotubes (MCNTs) with B/N co-doping. We show the formation energies which suggest that the B/N co-doping configuration is the energetically stable structure. We find that the electronic structure properties depend on the doping concentration of MCNTs, as well as the doping position. Energy gap opens rapidly when the symmetry breaking of MCNTs happens. These unconventional eloping effects could be used to design novel nanoelectronic devices. (c) 2010 Elsevier B.V. All rights reserved.
摘要：
14-3-3 sigma is a potential tumor suppressor, and loss of 14-3-3 sigma expression plays an important role in carcinogenesis and metastasis. To explore the possible mechanism of 14-3-3 sigma in nasopharyngeal carcinoma (NPC) invasion and metastasis, targeted proteomic analysis was performed on 14-3-3 sigma-associated proteins from NPC cells. As the results, 112 proteins associated with 14-3-3 sigma were identified, and four 14-3-3 sigma-interacted proteins: keratin 8, epidermal growth factor receptor (EGFR), small GTP-binding protein RAB7, and p53 were confirmed by coimmunoprecipitation and Western blot analysis. The 14-3-3 sigma-associated proteins could be grouped into eight clusters based on their molecule functions. Protein-protein interaction (PPI) analysis indicated that 14-3-3 sigma/EGFR/keratin 8 interactions may be involved in the invasion and metastasis of NPC. 14-3-3 sigma/EGFR/keratin 8 could form complexes in NPC cells. 14-3-3 sigma downregulation in NPC may lead to the overexpression of EGFR and keratin 8, which increases the invasion ability of NPC cells possibly by activating the downstream signal molecules and reorganizing cytoskeleton. The data suggest that the biological functions of 14-3-3 sigma in NPC are diversified, and 14-3-3 sigma could inhibit the in vitro invasive ability of NPC cells possibly through 14-3-3 sigma/EGFR/keratin 8 interaction. Crown Copyright (C) 2009 Published by Elsevier Ltd. All rights reserved.
通讯机构：
[Yin, Weidong] U;Univ S China, Life Sci Res Ctr, Inst Cardiovasc Res, Key Lab Atherosclerol Hunan Prov, Hengyang 421001, Hunan, Peoples R China.
摘要：
Insulin resistance and dyslipidemia are both considered to be risk factors for metabolic syndrome. Low levels of IGF1 are associated with insulin resistance. Elevation of low-density lipoprotein cholesterol (LDL-C) concomitant with depression of high-density lipoprotein cholesterol (HDL-C) increase the risk of obesity and type 2 diabetes mellitus (T2DM). Liver secretes IGF1 and catabolizes cholesterol regulated by the rate-limiting enzyme of bile acid synthesis from cholesterol 7α-hydroxylase (CYP7A1). NO-1886, a chemically synthesized lipoprotein lipase activator, suppresses diet-induced insulin resistance with the improvement of HDL-C. The goal of the present study is to evaluate whether NO-1886 upregulates IGF1 and CYP7A1 to benefit glucose and cholesterol metabolism. By using human hepatoma cell lines (HepG2 cells) as an in vitro model, we found that NO-1886 promoted IGF1 secretion and CYP7A1 expression through the activation of signal transducer and activator of transcription 5 (STAT5). Pretreatment of cells with AG 490, the inhibitor of STAT pathway, completely abolished NO-1886-induced IGF1 secretion and CYP7A1 expression. Studies performed in Chinese Bama minipigs pointed out an augmentation of plasma IGF1 elicited by a single dose administration of NO-1886. Long-term supplementation with NO-1886 recovered hyperinsulinemia and low plasma levels of IGF1 suppressed LDL-C and facilitated reverse cholesterol transport by decreasing hepatic cholesterol accumulation through increasing CYP7A1 expression in high-fat/high-sucrose/high-cholesterol diet minipigs. These findings indicate that NO-1886 upregulates IGF1 secretion and CYP7A1 expression to improve insulin resistance and hepatic cholesterol accumulation, which may represent an alternative therapeutic avenue of NO-1886 for T2DM and metabolic syndrome. Abstract Insulin resistance and dyslipidemia are both considered to be risk factors for metabolic syndrome. Low levels of IGF1 are associated with insulin resistance. Elevation of low-density lipoprotein cholesterol (LDL-C) concomitant with depression of high-density lipoprotein cholesterol (HDL-C) increase the risk of obesity and type 2 diabetes mellitus (T2DM). Liver secretes IGF1 and catabolizes cholesterol regulated by the rate-limiting enzyme of bile acid synthesis from cholesterol 7α-hydroxylase (CYP7A1). NO-1886, a chemically synthesized lipoprotein lipase activator, suppresses diet-induced insulin resistance with the improvement of HDL-C. The goal of the present study is to evaluate whether NO-1886 upregulates IGF1 and CYP7A1 to benefit glucose and cholesterol metabolism. By using human hepatoma cell lines (HepG2 cells) as an in vitro model, we found that NO-1886 promoted IGF1 secretion and CYP7A1 expression through the activation of signal transducer and activator of transcription 5 (STAT5). Pretreatment of cells with AG 490, the inhibitor of STAT pathway, completely abolished NO-1886-induced IGF1 secretion and CYP7A1 expression. Studies performed in Chinese Bama minipigs pointed out an augmentation of plasma IGF1 elicited by a single dose administration of NO-1886. Long-term supplementation with NO-1886 recovered hyperinsulinemia and low plasma levels of IGF1 suppressed LDL-C and facilitated reverse cholesterol transport by decreasing hepatic cholesterol accumulation through increasing CYP7A1 expression in high-fat/high-sucrose/high-cholesterol diet minipigs. These findings indicate that NO-1886 upregulates IGF1 secretion and CYP7A1 expression to improve insulin resistance and hepatic cholesterol accumulation, which may represent an alternative therapeutic avenue of NO-1886 for T2DM and metabolic syndrome.
关键词：
Atomic force microscopy (AFM);Dislocation;GaN;X-ray diffraction (XRD)
摘要：
The effect of the coalescence of islands on threading dislocations (TDs) in GaN films (300 nm thick) grown on non-annealed and annealed sapphire substrates has been studied. Atomic force microscopy measurements show that the a-type TD density first decreases and then increases during the coalescence process, while the densities of (a + c)- and c-type TDs decrease as coalescence proceeds. X-ray diffraction data indicate that the lattice tilt of GaN films is greatly reduced by coalescence while the change in twist depends on the degree of coalescence. (C) 2010 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.
摘要：
To investigate whether apelin-13 induced THP-1 monocytes (MCs) adhesion to ECV304 human umbilical vein endothelial cells (HUVECs) via 14-3-3 signaling transduction pathway and the potential novel physiological function and signaling transduction pathway of apelin-APJ, HUVECs ECV304 were cultured in DMEM and MCs THP-1 were cultured in RPMI 1640 medium. Monocyte adhesion and the expression of vascular cell adhesion molecule-1 (VCAM-1) and 14-3-3 were measured with monocyte adhesion assay and western blot analysis. Data showed that apelin-13 increased adhesion of MCs to HUVECs in a concentration- and time-dependent manner, which reached their peaks at 1 µM and 12 h, respectively. Similarly, apelin-13 induced the expression of HUVECs adhesion molecule, VCAM-1, in a concentration- and time-dependent manner, reached their peaks at 1 µM and 12 h, respectively. Apelin-13 induced the expression of 14-3-3 in a concentration- and time-dependent manner, which reached their peaks at 1 µM and 5 min, respectively. Furthermore, the potent 14-3-3 inhibitor difopein significantly reduced the expression of 14-3-3 and VCAM-1 in apelin-13 stimulated HUVECs, and difopein significantly inhibited the effect of apelin-13 on induction of MCs adhesion to HUVECs. These data suggested that 14-3-3 mediated the induction of adhesion of MCs to HUVECs by Apelin-13.
期刊：
Biochemical and Biophysical Research Communications,2010年391(4):1693-1697 ISSN：0006-291X
通讯作者：
Luo, Di-xian
作者机构：
[Liao, Duan-fang; Xia, Chenglai; Luo, Di-xian; Li, Junmo; Wang, Chun; Xu, Canxin; Zhu, Bingyang] Univ S China, Res Ctr Life Sci, Inst Pharm & Pharmacol, Div Pharmacoproteom, Hengyang 421001, Hunan, Peoples R China.;[Hu, Zhuowei] Peking Union Med Coll, Beijing 100730, Peoples R China.;[Hu, Zhuowei] Chinese Acad Med Sci, Inst Mat Med, Beijing 100730, Peoples R China.;[Luo, Di-xian] First Peoples Hosp Chenzhou City, Chenzhou 421001, Hunan, Peoples R China.;[Xiong, Yan; Luo, Di-xian] Cent S Univ, Sch Pharmaceut, Dept Pharmacol, Changsha 410083, Hunan, Peoples R China.
通讯机构：
[Luo, Di-xian] U;Univ S China, Res Ctr Life Sci, Inst Pharm & Pharmacol, Div Pharmacoproteom, Hengyang 421001, Hunan, Peoples R China.
摘要：
The title compound, C21H30O, was isolated from the soft coral Sinularia sp. The molecule contains four alicyclic rings, all trans-fused, among which three six-membered rings are in different distorted chair conformations while a five-membered ring assumes an envelope form.
作者：
Guo, Wei*;Wei, Shanshan;Lu, Xingqiang;Wang, Li
期刊：
Bulletin of the Korean Chemical Society,2010年31(12):3693-3696 ISSN：1229-5949
通讯作者：
Guo, Wei
作者机构：
[Guo, Wei; Wei, Shanshan] Univ S China, Sch Elect Engn, Hengyang 421001, Peoples R China.;[Lu, Xingqiang] Univ S China, Sch Nucl Sci & Technol, Hengyang 421001, Peoples R China.;[Wang, Li] Dalian Inst Chem Phys, State Key Lab Mol React Dynam, Dalian 116023, Peoples R China.
通讯机构：
[Guo, Wei] U;Univ S China, Sch Elect Engn, Hengyang 421001, Peoples R China.
关键词：
Two-step model;Rydberg states;Ponderomotive potential;Resonant/nonresonant population transfer
摘要：
We experimentally measure the kinetic energy and angular distributions of photoelectrons of N2 as a function of 410 nm femtosecond laser intensity by using velocity map imaging technique. The strong-field multiphoton ionization of molecules shares many of the characteristics with those of atoms. Electron kinetic energies are nearly independent of laser intensities. The independence suggests that the electron peaks in the photoelectron spectrum actually result from a two-step process, indicative of the occurrence of real population in the intermediate states. The relative amplitudes of electron peaks indicate that in the two-step process, nonresonant population transfer dominates for low intensities, while resonant population transfer dominates for higher intensities.
