期刊：
Lipids in Health and Disease,2010年9(1):1-10 ISSN：1476-511X
通讯作者：
Qin, Yang
作者机构：
[Qin, Yang; Tian, Li; Liu, Yinghui] Sichuan Univ, Dept Biochem & Mol Biol, W China Sch Preclin & Forens Med, Chengdu 610041, Sichuan, Peoples R China.;[Fu, Mingde] Sichuan Univ, Lab Endocrinol & Metab, W China Hosp, Chengdu 610041, Sichuan, Peoples R China.;[Long, Shiyin] Univ S China, Dept Biochem & Mol Biol, Hengyang 421001, Peoples R China.;[Xu, Yanhua] Chengdu Hoist Biotechnol Co LTD, Chengdu 610075, Peoples R China.
通讯机构：
[Qin, Yang] S;Sichuan Univ, Dept Biochem & Mol Biol, W China Sch Preclin & Forens Med, Chengdu 610041, Sichuan, Peoples R China.
关键词：
Reverse Cholesterol Transport;Cholesterol Ester Transfer Protein;Quebec Cardiovascular Study;West China Medical;Monitor Atherosclerosis Regression Study
摘要：
To evaluate the relationship between the low-density lipoprotein cholesterol (LDL-C)/high-density lipoprotein cholesterol (HDL-C) ratio and HDL subclass distribution and to further examine and discuss the potential impact of LDL-C and HDL-C together with TG on HDL subclass metabolism. Small-sized preβ1-HDL, HDL3b and HDL3a increased significantly while large-sized HDL2a and HDL2b decreased significantly as the LDL-C/HDL-C ratio increased. The subjects in low HDL-C level (< 1.03 mmol/L) who had an elevation of the LDL-C/HDL-C ratio and a reduction of HDL2b/preβ1-HDL regardless of an undesirable or high LDL-C level. At desirable LDL-C levels (< 3.34 mmol/L), the HDL2b/preβ1-HDL ratio was 5.4 for the subjects with a high HDL-C concentration (≥ 1.55 mmol/L); however, at high LDL-C levels (≥ 3.36 mmol/L), the ratio of LDL-C/HDL-C was 2.8 in subjects, and an extremely low HDL2b/preβ1-HDL value although with high HDL-C concentration. With increase of the LDL-C/HDL-C ratio, there was a general shift toward smaller-sized HDL particles, which implied that the maturation process of HDL was blocked. High HDL-C concentrations can regulate the HDL subclass distribution at desirable and borderline LDL-C levels but cannot counteract the influence of high LDL-C levels on HDL subclass distribution.
摘要：
Cholesterol efflux from lipid-loaded cells is a key athero-protective event that counteracts cholesterol uptake. The imbalance between cholesterol efflux and uptake determines the prevention or development of atherosclerosis. Many proteins and factors participate in the cholesterol efflux event. However, there are currently no systematic models of reverse cholesterol transport (RCT) that include most RCT-related factors and events. On the basis of recent research findings from other and our laboratories, we propose a novel model of one center and four systems with coupling transportation and networking regulation. This model represents a common way of cholesterol efflux; however, the systems in the model consist of different proteins/factors in different cells. In this review, we evaluate the novel model in vascular smooth muscle cells (VSMCs) and macrophages, which are the most important original cells of foam cells. This novel model consists of 1) a caveolae transport center, 2) an intracellular trafficking system of the caveolin-1 complex, 3) a transmembrane transport system of the ABC-A1 complex, 4) a transmembrane transport system of the SR-B1 complex, and 5) an extracelluar trafficking system of HDL/Apo-A1. In brief, the caveolin-1 system transports cholesterol from intracellular compartments to caveolae. Subsequently, both ABC-A1 and SR-B1 complex systems transfer cholesterol from caveolae to extracellular HDL/Apo-A1. The four systems are linked by a regulatory network. This model provides a simple and concise way to understand the dynamic process of atherosclerosis.
期刊：
Journal of Histochemistry & Cytochemistry,2010年58(6):517-527 ISSN：0022-1554
通讯作者：
Chen, Zhuchu
作者机构：
[Li, Maoyu; Xiao, Zhiqiang; Li, Cui; Li, Feng; Peng, Fang; Xiao, Zhefeng; Chen, Zhuchu; Li, Guoqing] Cent S Univ, Xiangya Hosp, Key Lab Canc Prote, Chinese Minist Hlth, Changsha 410008, Hunan, Peoples R China.;[Yu, Yanhui; Li, Feng; Ouyang, Yongmei; Xiao, Zhefeng] Cent S Univ, Canc Res Inst, Xiangya Sch Med, Changsha 410008, Hunan, Peoples R China.;[Li, Guoqing] Univ S China, Sch Pharm & Life Sci, Dept Biol, Hengyang, Hunan, Peoples R China.;[Chen, Yongheng] Univ So Calif, Los Angeles, CA USA.;[Chen, Zhuchu] Cent S Univ, Xiangya Hosp, Key Lab Canc Prote, Chinese Minist Hlth, 87 Xiangya Rd, Changsha 410008, Hunan, Peoples R China.
通讯机构：
[Chen, Zhuchu] C;Cent S Univ, Xiangya Hosp, Key Lab Canc Prote, Chinese Minist Hlth, 87 Xiangya Rd, Changsha 410008, Hunan, Peoples R China.
关键词：
NPC;FFPE;iTRAQ;2D LC-MS/MS
摘要：
Formalin-fixed, paraffin-embedded (FFPE) tissue specimens represent a potentially valuable resource for protein biomarker investigations. In this study, proteins were extracted by a heat-induced antigen retrieval technique combined with a retrieval solution containing 2% SDS from FFPE tissues of normal nasopharyngeal epithelial tissues (NNET) and three histological types of nasopharyngeal carcinoma (NPC) with diverse differentiation degrees. Then two-dimensional liquid chromatography-tandem mass spectrometry coupled with isobaric tags for relative and absolute quantification (iTRAQ) labeling was employed to quantitatively identify the differentially expressed proteins among the types of NPC FFPE tissues. Our study resulted in the identification of 730 unique proteins, the distributions of subcellular localizations and molecular functions of which were similar to those of the proteomic database of human NPC and NNET that we had set up based on the frozen tissues. Additionally, the relative expression levels of cathepsin D, keratin8, SFN, and stathmin1 identified and quantified in this report were consistent with the immunohistochemistry results acquired in our previous study. In conclusion, we have developed an effective approach to identifying protein changes in FFPE NPC tissues utilizing iTRAQ technology in conjunction with an economical and easily accessible sample preparation method. (J Histochem Cytochem 58:517-527, 2010)
摘要：
AIM: To investigate whether the apoptotic activities of 8-bromo-7-methoxychrysin (BrMC) involve reactive oxygen species (ROS) generation and c-Jun N-terminal kinase (JNK) activation in human hepatocellular carcinoma cells (HCC). METHODS: HepG2, Bel-7402 and L-02 cell lines were cultured in vitro and the apoptotic effects of BrMC were evaluated by flow cytometry (FCM) after propidium iodide (PI) staining, caspase-3 activity using enzyme-linked immunosorbent assay (ELISA), and DNA agarose gel electrophoresis. ROS production was evaluated by FCM after dichlorodihydrofluorescein diacetate (DCHFDA) probe labeling. The phosphorylation level of JNK and c-Jun protein was analyzed by Western blotting. RESULTS: FCM after PI staining showed a dose-dependent increase in the percentage of the sub-G1 cell population (P < 0.05), reaching 39.0% +/- 2.8% of HepG2 cells after 48 h of treatment with BrMC at 10 mu mol/L. The potency of BrMC to HepG2 and Bel-7402 (32.1% +/- 2.6%) cells was found to be more effective than the lead compound, chrysin (16.2% +/- 1.6% for HepG2 cells and 11.0% +/- 1.3% for Bel-7402 cell) at 40 mu mol/L and similar to 5-flurouracil (33.0% +/- 2.1% for HepG2 cells and 29.3% +/- 2.3% for Bel-7402 cells) at 10 mu mol/L. BrMC had little effect on human embryo liver L-02 cells, with the percentage of sub-G1 cell population 5.4% +/- 1.8%. Treatment of HepG2 cells with BrMC for 48 h also increased the levels of active caspase-3, in a concentration-dependent manner. z-DEVD-fmk, a caspase-3-specific inhibitor, prevented the activation of caspase-3. Treatment with BrMC at 10 mu mol/L for 48 h resulted in the formation of a DNA ladder. Treatment of cells with BrMC (10 mu mol/L) increased mean fluorescence intensity of DCHF-DA in HepG2 cells from 7.2 +/- 1.12 at 0 h to 79.8 +/- 3.9 at 3 h and 89.7 +/- 4.7 at 6 h. BrMC did not affect ROS generation in L-02 cells. BrMC treatment failed to induce cell death and caspase-3 activation in HepG2 cells pretreated with N-acetylcysteine (10 mmol/L). In addition, in HepG2 cells treated with BrMC (2.5, 5.0, 10.0 mu mol/L) for 12 h, JNK activation was observed. Peak JNK activation occurred at 12 h post-treatment and this activation persisted for up to 24 h. The expression of phosphorylated JNK and c-Jun protein after 12 h with BrMC-treated cells was inhibited by N-acetylcysteine and SP600125 pre-treatment, but GW9662 had no effect. SP600125 substantially reduced BrMC-induced cell death and caspase-3 activation of HepG2 cells. N-acetylcysteine and GW9662 also attenuated induction of cell death and caspase-3 activation in HepG2 cells treated with BrMC. CONCLUSION: BrMC induces apoptosis of HCC cells by ROS generation and sustained JNK activation. (C) 2010 Baishideng. All rights reserved.
摘要：
Air-stable hypervalent organobismuth(III) tetrafluoroborate (C6H11N(CH2C6H4)2BiBF4) was synthesized and characterized by spectroscopic and X-ray crystallographic techniques. The compound shows good catalytic efficiency in the allylation reaction of different aldehydes with tetraallyltin in a medium of aqueous methanol, giving the corresponding homoallylic alcohols in excellent chemoselectivity and yields.
作者：
Ling, H.;Wen, L.;Ji, X. X.;Tang, Y. L.;He, J.;...
期刊：
Brazilian Journal of Medical and Biological Research,2010年43(3):271-278 ISSN：0100-879X
通讯作者：
Su, Q.
作者机构：
[Ji, X. X.; Su, Q.; He, J.; Xia, H.; Tang, Y. L.; Ling, H.; Wen, L.; Zhou, J. G.; Tan, H.] Univ S China, Canc Res Inst, Hengyang 421001, Hunan, Peoples R China.;[Su, Q.] Univ S China, Canc Res Inst, 28 Changshengxi Rd, Hengyang 421001, Hunan, Peoples R China.
通讯机构：
[Su, Q.] U;Univ S China, Canc Res Inst, 28 Changshengxi Rd, Hengyang 421001, Hunan, Peoples R China.
关键词：
Diallyl disulfide;Cell cycle G(2)/M arrest;Checkpoint kinase-1;Gastric cancer
摘要：
Diallyl disulfide (DADS) inhibits growth and induces cell cycle G2/M arrest in human gastric cancer MGC803 cells. In this study, 15 mg/L DADS exerted similar effects on growth and cell cycle arrest in human gastric cancer BGC823 cells. Due to the importance of cell cycle redistribution in DADS-mediated anti-carcinogenic effects, we investigated the role of checkpoint kinases (Chk1 and Chk2) during DADS-induced cell cycle arrest. We hypothesized that DADS could mediate G2/M phase arrest through either Chk1 or Chk2 signal transduction pathways. We demonstrated that DADS induced the accumulation of phosphorylated Chk1, but not of Chk2, and that DADS down-regulated Cdc25C and cyclin B1. The expression of mRNA and total protein for Chkl and Chk2 was unchanged. Chk1 is specifically phosphorylated by ATR (ATM-RAD3-related gene). Western blot analysis showed that phospho-ATR was activated by DADS. Taken together, these data suggest that cell cycle G2/M arrest, which was associated with accumulation of the phosphorylated forms of Chk1, but not of Chk2, was involved in the growth inhibition induced by DADS in the human gastric cancer cell line BGC823. Furthermore, the DADS-induced G2/M checkpoint response is mediated by Chk1 signaling through ATR/Chk1/Cdc25C/cyclin B1, and is independent of Chk2.
摘要：
OBJECTIVES To determine the systemic response to percutaneous nephrolithotomy (PCNL) and mini-PCNL (MPCNL) and evaluate whether MPCNL is less invasive than PCNL, as experimental studies suggest that the acute-phase reaction is proportional to surgery-induced tissue damage. METHODS In all, 165 consecutive patients who had undergone MPCNL (93) or PCNL (72) were prospectively assessed. Blood samples were collected 24 hours before; during surgery; at the end of anesthesia; and 12, 24, and 36 hours after surgery. The extent of the systemic response to surgery-induced tissue trauma was measured, by assessing the levels of acute-phase markers tumor necrosis factor-alpha (TNF-alpha), interleukin-6 (IL-6), IL-10, C-reactive protein (CRP), and serum amyloid A (SAA), at all sampling times in all patients. RESULTS No significant differences were observed between the 2 groups in preoperative variables. Baseline levels of TNF-alpha, IL-6, IL-10, CRP, and SAA were comparable in both groups. An increase was noted in TNF-alpha, IL-6, CRP, and SAA after surgery but no significant differences were assessed between MPCNL and PCNL during the entire period. IL-10 did not change at the different sampling times. CONCLUSIONS Our data fail to demonstrate significant advantages of MPCNL in terms of reduced surgical trauma and associated invasiveness compared with standard PCNL based on the variables objectively measured in this study. UROLOGY 75: 56-61, 2010. (C) 2010 Elsevier Inc.
作者机构：
[Ren, Yan-Kai; Chen, Rong-Qian; Tang, Xiao-Qing; Hu, Bi; He, Jian-Qin; Shen, Xin-Tian; Yin, Wei-Lan] Univ S China, Dept Physiol, Coll Med, Hengyang 421001, Hunan, Peoples R China.;[Jiang, Zhi-Sheng] Univ S China, Dept Pathophysiol, Coll Med, Hengyang 421001, Hunan, Peoples R China.;[Shen, Xin-Tian; Huang, Yi-E] Huaihua Med Coll, Dept Physiol, Huaihua 418000, Hunan, Peoples R China.;[Xu, Jin-Hua] Univ S China, Lab Ctr Biochem & Mol Biol, Hengyang 421001, Hunan, Peoples R China.;[Tang, Xiao-Qing] Univ S China, Dept Physiol, Coll Med, 28 W Changsheng Rd, Hengyang 421001, Hunan, Peoples R China.
通讯机构：
[Tang, Xiao-Qing] U;Univ S China, Dept Physiol, Coll Med, 28 W Changsheng Rd, Hengyang 421001, Hunan, Peoples R China.
关键词：
Bcl-2;Homocysteine;Hydrogen sulfide;Mitochondrial membrane potential;Neuroprotection;Reactive oxygen species
摘要：
Hydrogen sulfide (H(2)S) has been shown to protect neurons against oxidative stress. Lower levels of H(2)S as well as accumulation of homocysteine (Hcy), a strong risk of Alzheimer's disease (AD), are reported in the brains of AD patients. The aim of present study is to explore the protection of H(2)S against Hcy-induced cytotoxicity and apoptosis and the molecular mechanisms underlying in PC12 cells. We show that sodium hydrosulfide (NaHS), a H(2)S donor, protects PC12 cells against Hcy-mediated cytotoxicity and apoptosis by preventing both the loss of mitochondrial membrane potential (MMP) and the increase in intracellular reactive oxygen species (ROS) induced by Hcy. NaHS not only promotes the expression of bc1-2, but also blocks the down-regulation of bc1-2 by Hcy. These results indicate that H(2)S protects neuronal cells against neurotoxicity of Hcy by preserving MMP and attenuating ROS accumulation through up-regulation of bcl-2 level. Our study suggests a promising future of H(2)S-based therapies for neurodegenerative diseases such as AD. (C) 2010 Elsevier Ireland Ltd and the Japan Neuroscience Society. All rights reserved.
作者：
He Qing-Zhi;Tuo Qin-Hui;Zeng Huai-Cai;Zhu Bing-Yang;Rang Wei-Qing;...
期刊：
生物化学与生物物理进展,2010年37(8):881-890 ISSN：1000-3282
通讯作者：
Tang Xiao-Qing
作者机构：
[Zhu Bing-Yang; Tuo Qin-Hui; Liao Duan-Fang; He Qing-Zhi] Univ S China, Prov Key Lab Pharmacoprotom, Inst Pharm & Pharmacol, Hengyang 421001, Peoples R China.;[Tang Xiao-Qing] Univ S China, Dept Physiol, Sch Med, Hengyang 421001, Peoples R China.;[Liao Duan-Fang] Hunan Univ Chinese Med, Dept Tradit Chinese Diagnot, Sch Pharm, Changsha 410208, Hunan, Peoples R China.;[Rang Wei-Qing; Zeng Huai-Cai] Univ S China, Sch Publ Hlth, Hengyang 421001, Peoples R China.
通讯机构：
[Tang Xiao-Qing] U;Univ S China, Dept Physiol, Sch Med, Hengyang 421001, Peoples R China.
关键词：
Daxx;Ox-LDL;macrophage;apoptosis;caveolin-1
摘要：
To explore whether Daxx mediates oxidized low-density lipoprotein (Ox-LDL)-induced cholesterol accumulation and apopotosis in macrophage and the underlying molecular mechanisms, intracellular lipid droplets and lipid levels were assayed by oil red O staining and high performance liquid chromatography (HPLC), respectively, the apoptotic effect of RAW264.7 cells induced by Ox-LDL was analyzed by flow cytometric analysis and acridine orange/ethidium bromide (AO/EB) staining, the mRNA expressions of Daxx was quantified by Real time RT- PCR, the protein expression of caveolin-1 was detected by Western-blotting, Daxx-specific small interfering RNA(Daxx siRNA) was transfected to RAW264.7 cell by lipofectamin. Ox-LDL up-regulated the expression of Daxx mRNA, increased the accumulation of intercellular cholesterol in RAW264.7 macrophages, and induced the apoptosis of RAW264.7 macrophages. However, Ox-LDL-induced intercellular cholesterol accumulation and apoptosis in RAW264.7 cells was prevented by Daxx siRNA. Ox-LDL also induced caveolin-1 expression and this effect is significantly suppressed by Daxx siRNA. It can be concluded that Daxx mediates Ox-LDL-induced cholesterol accumulation and apoptosis in macrophages by up-regulating caveolin-1 expression. These findings provide an important demonstration that Daxx might be associated with the development of atherosclerosis.
摘要：
通过研究大气颗粒物中~(222)Rn子体活度比与颗粒物平均寿命之间的关系,建立了基于活度比估算大气颗粒物滞留时间(residence time of atmospheric aerosol,RTAA)的方法,并在相对理想条件下进行了实验验证.当氡室浓度稳定为1.816 kBq/m~3时,由214Bi/214Pb活度比计算出的RTAA为112.17 min,与氡室的平均换气时间(104.17 min)相当,表明大气颗粒物中同一衰变链上的放射性核素活度比(如~(214)Bi/~(214)Pb, ~(210)Bi/~(210)Pb或~(210)Po/~(210)Pb)可以用于估算RTAA.
摘要：
Genistein has been shown to increase nitric oxide (NO) production derived from endothelial nitric oxide synthase (eNOS). This study was to investigate whether genistein could prevent myocardial hypertrophy in the 2-kidney 1-clip (2K1C) renohypertensive rat through the NO pathway and to clarify the underlying mechanisms. After the 2K1C operation, plasma angiotensin II increased, and the rats developed significant left ventricular hypertrophy (LVH) and increased collagen I expression. Phosphorylated eNOS, NOS activity, NO production and cGMP contents were markedly decreased in ventricular tissues of 2K1C rats. Chronic administration of genistein to 2K1C rats restored NO, NOS activity, phosphorylated eNOS expression, cGMP in ventricular tissues, and the restoration was parallel with the improvement of LVH and attenuated the excessive ventricular collagen I expression. Genistein also elevated angiotensin II type 2 receptor (AT2) expression, and the effects of genistein on LVH could be completely abolished by an AT2 antagonist, PD123319. The antagonist also reversed the increase in eNOS activity, NO and cGMP restored by genistein in hypertensive rats. We further explored the mechanisms by which genistein restored NO in hypertension and found that genistein significantly enhanced phosphorylated eNOS but left relatively unchanged total eNOS and the eNOS dimer/monomer ratio. In addition, genistein decreased the binding of eNOS with caveolin 3 and simultaneously promoted its binding with calmodulin and heat shock protein 90. We conclude that the preventive effects of genistein on cardiac remodeling induced by 2K1C hypertension are mediated by AT2-dependent NO production.
作者：
Hao, Qing;Gao, Shan;Leung, Thomas Wai Hong;Guo, Ming Hui;You, Yong;...
期刊：
Journal of Neuroimaging,2010年20(2):122-129 ISSN：1051-2284
通讯作者：
Wong, Ka Sing
作者机构：
[Guo, Ming Hui; Hao, Qing; You, Yong; Wong, Ka Sing] Chinese Univ Hong Kong, Dept Med & Therapeut, Shatin, Hong Kong, Peoples R China.;[Gao, Shan] Beijing Union Med Coll Hosp, Dept Neurol, Beijing, Peoples R China.;[Gao, Shan] CAMS, Beijing, Peoples R China.;[You, Yong] Nanhua Univ, Affiliated Hosp 1, Dept Neurol, Hengyang, Peoples R China.
通讯机构：
[Wong, Ka Sing] C;Chinese Univ Hong Kong, Dept Med & Therapeut, Shatin, Hong Kong, Peoples R China.
摘要：
This paper is concerned with some sequences based on multiplicative convexity. Our results yield a class of new inequalities between ratios and differences of means, some of which extend the known ones.
作者机构：
[Xu, Xiaona; Liang, Yizeng] Cent S Univ, Coll Chem & Chem Engn, Res Ctr Modernizat Chinese Tradit & Herbal Drug M, Changsha 410083, Hunan, Peoples R China.;[Xu, Xiaona] Univ S China, Coll Chem & Chem Engn, Hengyang, Hunan, Peoples R China.;[Tang, Zhonghai] Hunan Agr Univ, Biosci & Biotechnol Coll, Changsha 410128, Hunan, Peoples R China.
通讯机构：
[Liang, Yizeng] C;Cent S Univ, Coll Chem & Chem Engn, Res Ctr Modernizat Chinese Tradit & Herbal Drug M, Changsha 410083, Hunan, Peoples R China.
摘要：
The aim of this study was to compare the expression of annexin II (ANXA2) in benign prostatic hyperplasia (BPH) with that of prostate cancer (PC), and to correlate the expression levels with pathologic grade and stage. Immunohistochemistry was performed on samples from 85 patients with PC and 40 patients with BPH. The correlation between ANXA2 expression and clinicopathologic features and clinical outcome was evaluated. The data showed that ANXA2 expression was significantly lower in PC compared to BPH (P<0.01). There was significant difference between ANXA2 expression and Gleason score (P<0.01). Patients with down-regulated ANXA2 tended to have tumors of advanced clinical stage, more frequent recurrence and regional lymph node and distant metastasis. ANXA2 expression was not correlated with age. The down-regulation of ANXA2 in a PC-3 cell line increased in vitro invasive ability, and ANXA2 had an independent prognostic effect on overall survival. In conclusion, ANXA2 dysregulation is an important event associated with the development and progression of PC. ANXA2 down-regulation aids in the discrimination of PC from BPH and may serve as a clinically useful biomarker.
