Quantitative Proteomic Analysis of Formalin-fixed and Paraffin-embedded Nasopharyngeal Carcinoma Using iTRAQ Labeling, Two-dimensional Liquid Chromatography, and Tandem Mass Spectrometry

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作者信息关键词期刊信息基础信息归属信息摘要

成果类型：

期刊论文

作者：

Xiao, Zhefeng;Li, Guoqing;Chen, Yongheng;Li, Maoyu;Peng, Fang;...

通讯作者：

Chen, Zhuchu

作者机构：

[Li, Maoyu; Xiao, Zhiqiang; Li, Cui; Li, Feng; Peng, Fang; Xiao, Zhefeng; Chen, Zhuchu; Li, Guoqing] Cent S Univ, Xiangya Hosp, Key Lab Canc Prote, Chinese Minist Hlth, Changsha 410008, Hunan, Peoples R China.

[Yu, Yanhui; Li, Feng; Ouyang, Yongmei; Xiao, Zhefeng] Cent S Univ, Canc Res Inst, Xiangya Sch Med, Changsha 410008, Hunan, Peoples R China.

[Li, Guoqing] Univ S China, Sch Pharm & Life Sci, Dept Biol, Hengyang, Hunan, Peoples R China.

[Chen, Yongheng] Univ So Calif, Los Angeles, CA USA.

[Chen, Zhuchu] Cent S Univ, Xiangya Hosp, Key Lab Canc Prote, Chinese Minist Hlth, 87 Xiangya Rd, Changsha 410008, Hunan, Peoples R China.

通讯机构：

[Chen, Zhuchu] C

Cent S Univ, Xiangya Hosp, Key Lab Canc Prote, Chinese Minist Hlth, 87 Xiangya Rd, Changsha 410008, Hunan, Peoples R China.

语种：

英文

关键词：

NPC;FFPE;iTRAQ;2D LC-MS/MS

期刊：

Journal of Histochemistry & Cytochemistry

ISSN：

0022-1554

年：

2010

卷：

期：

页码：

517-527

DOI：

10.1369/jhc.2010.955526

基金类别：

National Key Basic Research Program of ChinaNational Basic Research Program of China [2001CB510207]; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [30800419, 30973289]; Program for New Century Excellent Talents in UniversityProgram for New Century Excellent Talents in University (NCET) [NECT-07-0861]

机构署名：

本校为其他机构

院系归属：

药学与生物科学学院

摘要：

Formalin-fixed, paraffin-embedded (FFPE) tissue specimens represent a potentially valuable resource for protein biomarker investigations. In this study, proteins were extracted by a heat-induced antigen retrieval technique combined with a retrieval solution containing 2% SDS from FFPE tissues of normal nasopharyngeal epithelial tissues (NNET) and three histological types of nasopharyngeal carcinoma (NPC) with diverse differentiation degrees. Then two-dimensional liquid chromatography-tandem mass spectrometry coupled with isobaric tags for relative and absolute quantification (iTRAQ) labeling w...

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Quantitative Proteomic Analysis of Formalin-fixed and Paraffin-embedded Nasopharyngeal Carcinoma Using iTRAQ Labeling, Two-dimensional Liquid Chromatography, and Tandem Mass Spectrometry

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