期刊论文

Cao, J.-G.

英文

世界胃肠病学杂志：英文版

1007-9327

2010

16

27

3385-3393

10.3748/wjg.v16.i27.3385

Hunan Provincial Natural Science Foundation of ChinaNatural Science Foundation of Hunan Province [03JJY5009]

本校为其他机构

AIM: To investigate whether the apoptotic activities of 8-bromo-7-methoxychrysin (BrMC) involve reactive oxygen species (ROS) generation and c-Jun N-terminal kinase (JNK) activation in human hepatocellular carcinoma cells (HCC). METHODS: HepG2, Bel-7402 and L-02 cell lines were cultured in vitro and the apoptotic effects of BrMC were evaluated by flow cytometry (FCM) after propidium iodide (PI) staining, caspase-3 activity using enzyme-linked immunosorbent assay (ELISA), and DNA agarose gel electrophoresis. ROS production was evaluated by FCM after dichlorodihydrofluorescein diacetate (DCHFDA)...

AIM: To investigate whether the apoptotic activities of 8-bromo-7-methoxychrysin (BrMC) involve reactive oxygen species (ROS) generation and c-Jun N-terminal kinase (INK) activation in human hepatocellular carcinoma cells (HCC). METHODS: HepG2, Bel-7402 and L-02 cell lines were cultured in vitro and the apoptotic effects of BrMC were evaluated by flow cytometry (FCM) after propidium iodide (PI) staining, caspase-3 activity using enzymelinked immunosorbent assay (ELISA), and DNA agarose gel electrophoresis. ROS production was evaluated by FCM af...