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Daxx Mediates Oxidized Low-density Lipoprotein-Induced Cholesterol Accumulation and Apoptosis in Macrophages by Upregulating Caveolin-1 Expression

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成果类型:
期刊论文
作者:
He Qing-Zhi;Tuo Qin-Hui;Zeng Huai-Cai;Zhu Bing-Yang;Rang Wei-Qing;...
通讯作者:
Tang Xiao-Qing
作者机构:
[Zhu Bing-Yang; Tuo Qin-Hui; Liao Duan-Fang; He Qing-Zhi] Univ S China, Prov Key Lab Pharmacoprotom, Inst Pharm & Pharmacol, Hengyang 421001, Peoples R China.
[Tang Xiao-Qing] Univ S China, Dept Physiol, Sch Med, Hengyang 421001, Peoples R China.
[Liao Duan-Fang] Hunan Univ Chinese Med, Dept Tradit Chinese Diagnot, Sch Pharm, Changsha 410208, Hunan, Peoples R China.
[Rang Wei-Qing; Zeng Huai-Cai] Univ S China, Sch Publ Hlth, Hengyang 421001, Peoples R China.
通讯机构:
[Tang Xiao-Qing] U
Univ S China, Dept Physiol, Sch Med, Hengyang 421001, Peoples R China.
语种:
英文
关键词:
Daxx;Ox-LDL;macrophage;apoptosis;caveolin-1
关键词(中文):
氧化型低密度脂蛋白;巨噬细胞;凋亡
期刊:
生物化学与生物物理进展
ISSN:
1000-3282
年:
2010
卷:
37
期:
8
页码:
881-890
基金类别:
National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [30572191, 30770868, 30971267, 30971170]
机构署名:
本校为第一且通讯机构
院系归属:
医学院
药学与生物科学学院
公共卫生学院
摘要:
To explore whether Daxx mediates oxidized low-density lipoprotein (Ox-LDL)-induced cholesterol accumulation and apopotosis in macrophage and the underlying molecular mechanisms, intracellular lipid droplets and lipid levels were assayed by oil red O staining and high performance liquid chromatography (HPLC), respectively, the apoptotic effect of RAW264.7 cells induced by Ox-LDL was analyzed by flow cytometric analysis and acridine orange/ethidium bromide (AO/EB) staining, the mRNA expressions of Daxx was quantified by Real time RT- PCR, the protein expression of caveolin-1 was detected by West...
摘要(中文):
为探讨Daxx对氧化型低密度脂蛋白(oxidized low-density lipoprotein,Ox-LDL)诱导巨噬细胞胆固醇蓄积和凋亡的介导作用及其可能的分子机制,用高效液相色谱法检测细胞内胆固醇含量,油红O染色观察细胞内脂滴的形成情况,流式细胞术和吖啶橙/溴化乙锭(AO/EB)染色法研究Ox-LDL对细胞凋亡的影响,Realtime RT-PCR检测细胞内Daxx mRNA的表达水平,Western blot检测caveolin-1蛋白的表达,用特异性siRNA沉默Daxx在RAW264.7细胞中的表达.Ox-LDL上调Daxx mRNA和caveolin-1的表达、增加细胞内胆固醇含量、促使RAW264.7细胞凋亡,用特异性siRNA干扰Daxx在RAW264.7细胞中的表达能降低caveolin-1的表达、减少细...

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