摘要：
With the development of science and technology, electronic learning has played a more and more important role in school teaching while the course resource platform of higher vocational colleges for nursing develops slowly. This paper analyzes theories of course resource platform from home and abroad, designing a virtual nursing course resource platform. This platform is divided into three modules-nursing teaching, database design and function, combining with data interaction, synchronization, analysis and processing to advance development of the platform. This paper also states problems in detail, like data and software update and gives a reference to the development of Internet teaching resource through the design of this platform.
期刊：
Environmental Toxicology and Pharmacology,2016年45:68-73 ISSN：1382-6689
通讯作者：
Zhou, Yan
作者机构：
[Zhou, Yan; Guo, Wei-Zhen; Zou, Xue-Min] Changsha Med Univ, Sch Publ Hlth, Changsha, Hunan, Peoples R China.;[Fu, Xiao-Ming] Hunan Normal Univ, Xiangnan Affiliated Hosp, Hosp Peoples Liberat Army 169, Hengyang, Hunan, Peoples R China.;[Wu, Cheng-Qiu; He, Dong-Liang] Univ South China, Sch Publ Hlth, Dept Prevent Med, Hengyang, Hunan, Peoples R China.;[He, Dong-Liang] Huazhong Univ Sci & Technol, Tongji Med Coll, Sch Publ Hlth, Dept Nutr & Food Hyg, Wuhan, Hubei, Peoples R China.;[Feng, Wei] Huazhong Univ Sci & Technol, Tongji Med Coll, Sch Publ Hlth, Dept Occupat & Environm Hlth, Wuhan, Hubei, Peoples R China.
通讯机构：
[Zhou, Yan] C;Changsha Med Univ, Sch Publ Hlth, Changsha, Hunan, Peoples R China.
关键词：
Comet assay;Male infertility;Male reproductive health;Metals;Sperm DNA damage
摘要：
This study aimed to examine associations between urinary metal concentrations and sperm DNA damage. Thirteen metals [arsenic (As), cadmium (Cd), cobalt (Co), chromium (Cr), copper (Cu), iron (Fe), lead (Pb), manganese (Mn), molybdenum (Mo), mercury (Hg), nickel (Ni), selenium (Se), and zinc (Zn)] were detected in urine samples of 207 infertile men from an infertility clinic using inductively coupled plasma mass spectrometry, and also, sperm DNA damage (tail length, percent DNA tail, and tail distributed moment) were assessed using neutral comet assay. We found that urinary Hg and Ni were associated with increasing trends for tail length (both p for trend < 0.05), and that urinary Mn was associated with increasing trend for tail distributed moment (p for trend = 0.02). These associations did persist even when considering multiple metals. Our results suggest that environmental exposure to Hg, Mn, and Ni may be associated with increased sperm DNA damage. (c) 2016 Elsevier B.V. All rights reserved.
期刊：
BioMed Research International,2015年2015:1-10 ISSN：2314-6133
通讯作者：
Zeng, Huai-cai
作者机构：
[Tan, Yan; Li, Wu; Wu, Cheng-qiu; Pan, Xiao-yuan; Shan, Xiao-yun; Zeng, Huai-cai; Wang, Jing] Univ South China, Sch Publ Hlth, Dept Prevent Med, Hengyang 421001, Peoples R China.;[He, Qing-zhi; Zeng, Huai-cai] Hunan Prov Cooperat Innovat Ctr Mol Target New Dr, Hengyang 421001, Peoples R China.;[Zeng, Huai-cai] Univ South China, Sch Publ Hlth, Dept Prevent Med, 28 Western Changsheng Rd, Hengyang 421001, Peoples R China.
通讯机构：
[Zeng, Huai-cai] U;Univ South China, Sch Publ Hlth, Dept Prevent Med, 28 Western Changsheng Rd, Hengyang 421001, Peoples R China.
摘要：
<jats:p>Perfluorooctane sulfonate (PFOS), a ubiquitous environmental pollutant, is neurotoxic to mammalian species. However, the underlying mechanism of its neurotoxicity was unclear. We hypothesized that PFOS suppresses BDNF expression to produce its neurotoxic effects by inhibiting the ERK-CREB pathway. SH-SY5Y human neuroblastoma cells were exposed to various concentrations of PFOS to examine the role of the BDNF-ERK-CREB signalling pathway in PFOS-induced apoptosis and cytotoxicity. Furthermore, to ascertain the mechanism by which PFOS reduces BDNF signalling, we examined the expression levels of miR-16 and miR-22, which potentially regulate BDNF mRNA translation at the posttranscriptional level. Results indicated that PFOS significantly decreased cell viability and induced apoptosis in SH-SY5Y cells. In addition, BDNF and pERK protein levels decreased after PFOS treatment; however, pCREB protein levels were significantly elevated in PFOS treated groups. TrkB protein expression increased in the 10 <jats:italic>μ</jats:italic>M and 50 <jats:italic>μ</jats:italic>M PFOS groups and significantly decreased in the 100 <jats:italic>μ</jats:italic>M PFOS group. Our results demonstrated that PFOS exposure decreased miR-16 expression and increased miR-22 expression, which may represent a possible mechanism by which PFOS decreases BDNF protein levels. PFOS may inhibit BDNF-ERK-CREB signalling by increasing miR-22 levels, which may, in part, explain the mechanism of PFOS neurotoxicity.</jats:p>
