期刊论文

中文

实用预防医学

1006-3110

2014

20

3

264-266

10.3969/j.issn.1006-3110.2013.03.003

湖南省自然科学基金（No:5-06-XI-06JJ50053）；

本校为第一机构

目的观察镍与铬对小鼠睾丸的联合毒作用,探讨镍、铬联合作用类型及毒作用机制。方法54只昆明种雄性小鼠按3×3析因设计随机等分为9组,镍染毒剂量为0.0,2.5、5.0mg/kg·bw、铬染毒剂量为0.0、2.5、5.0 mg/kg·bw,按0.1 ml/10 g·bw连续灌胃5d,35d后处死动物,检测睾丸脏器系数、睾丸结构、睾丸细胞凋亡及细胞周期;精子数量、活力和畸型;检测睾丸组织中MDA含量、SOD、LDH、SDH活性。结果小鼠睾丸脏器系数、SOD、LDH、SDH活性随染毒剂量增加而降低,MDA含量增高（P【0.01）,且有量一效关系;精子数量较对照组低（P【0.01）、精子活力下降（P【0.01）、畸形率高于对照（P【0.01）,睾丸的结构受损,各染毒...

Objective To study the synergistic toxicity of nickel and chromium in testis in mice,and to explore the type of the joint toxicity and its possible mechanisms.Methods Totally,54 male Kunming mice were randomly assigned into nine groups according to 3×3 factorial design.The doses of both nickel and chromium were0,2.5 and 5.0mg/kg.bw.Nickel andchromium were administered to mice once a day at a volume of 0.1ml/10 g.bw by gavage for a consecution of five days.After being fed for 35 days.the testes were removed.The testicular organ coefficient was ...