摘要：
In order to develop and apply mixed iron- and sulfur-oxidizers in uranium bioleaching, the characteristics of a mixed iron- and sulfur-oxidizing consortium (Consortium ISO) were comparatively investigated versus an iron-oxidizing consortium (Consortium IO). The results showed, the Consortium ISO exerted stronger oxidative ability and acid-producing ability than Consortium IO did. The synergy of sulfur-oxidizers and iron-oxidizers could change the structure and properties of the passivation substance, and work positively for eliminating the accumulation of passivation substance. In the bioleaching process, the uranium bioleaching experiments showed the recovery percentage of uranium reached 99.5% with Consortium ISO, 6.3% more than that of Consortium IO.
摘要：
A defined mixed bioleaching consortium (constructed by Acidithiobacills ferrooxidans ATCC 23270, Leptospirillum ferriphilum YSK, Sulfobacillus thermosulfidooxidans ST, Acidithiobacills thiooxidans A01 and Acidithiobacills caldus S1) was used to investigate the fluoride stress response. The results showed that its growth and iron oxidation rate were obviously inhibited, while the sulphur oxidation was only barely restrained. Furthermore, the community dynamics and gene expression were assayed respectively by using real-time quantitative polymerase chain reaction assay (RT-PCR) and functional gene arrays (FGAs). From the analysis of community dynamics, the most obviously inhibited strains were S. thermosulfidooxidans ST, while L. ferriphilum YSK still maintained stable growth. After 4.8 mM of fluoride stress for 28 h, the S. thermosulfidooxidans cell concentration was decreased by about 517.8 times more than in non-stress state. From the analysis of gene expression, fluoride stress was seen to exert pleiotropic effects on different species in the consortium. The dominant species played the pivotal role in resisting fluoride stress and maintaining activities in the system, yet the inferior species also had a critical function of assisting the survival of the dominant species.
作者机构：
[胡鄂明; 史文革; 李乾; 王清良; 丁德馨] School of Nuclear Resources Engineering, University of South China, Hengyang 421001, China;[李乾; 丁德馨] Key Discipline Lab. for National Defence of Biotechnology in Uranium Mining and Hydrometallurgy, University of South China, Hengyang 421001, China;[刘学端; 马丽媛] School of Minerals Processing and Bioengineering, Central South University, Changsha 410083, China
通讯机构：
School of Minerals Processing and Bioengineering, Central South University, China
期刊：
2011 International Conference on Computer Distributed Control and Intelligent Environmental Monitoring,2011年:2339-2342
作者机构：
[Cai Ping-li] School of Urban Construction, University of South China, Hengyang, China;[Shi Wen-ge] School of Nuclear Resources and Nuclear fuel Engineering, University of South China, Hengyang, China
会议名称：
2011 International Conference on Computer Distributed Control and Intelligent Environmental Monitoring
会议时间：
February 2011
会议地点：
Changsha, China
会议论文集名称：
2011 International Conference on Computer Distributed Control and Intelligent Environmental Monitoring
关键词：
acid in-situ leaching of uranium;groundwater;surface treatment;natural purificatioin
摘要：
The article discusses wall rock neutralization acid-in-situ leaching of uranium testing and pollution of groundwater purification combined with the natural purification of the indoor simulation tests. Test showed that the acidic groundwater can be neutralized by wall rock and the uranium can be also adsorbed. First, the uranium of contaminated groundwater be recovered by ion exchange, Second, regulated pH of wastewater to 7 around using neutralize, Thirdly, adsorbed by the Sand with clay, low grade uranium ore and tailings of lixiviated, the method is advanced which treatment groundwater of acid in-situ leaching of uranium using surface treatment and self purification, for acid in-situ leaching of uranium to provide a reference.
