期刊论文

Technology of immobilized acidithiobacillus ferrooxidans with SA-HPAM

中文

中国矿业

1004-4051

2016

25

3

150-153,157

10.3969/j.issn.1004-4051.2016.03.037

本校为第一机构

以海藻酸钠(SA)、聚丙烯酰胺(HPAM)为复合载体,加入聚乙二醇(PEG)为致孔剂,对氧化亚铁硫杆菌进行固定化研究.实验考察了SA、HPAM、PEG的浓度对SA-HPAM凝胶微球的机械强度及固定化细胞的氧化活性的影响,确定了三种材料的最佳浓度:SA为2.5%,HPAM为0.4%,PEG为2%,此条件下固定化细胞对Fe2+的最大氧化速率可达2.853 g·(L·h)-1,24h后Fe2+的氧化率在98%以上,固定化细胞目前重复使用了15次,并在3℃冰箱中存放两个月后,依然具有很好的氧化活性,表明S A-HPAM固定化的氧化亚铁硫杆菌具有较好的稳定性.

The SA and HPAM was used as compound carrier ,and the PEG was added as pore-foaming agent to study the immobilized technology of acidithiobacillus ferrooxidans .Effect of concentration of the three materials on the mechanical strength of gelatinous microspheres and the activity of immobilized cells have been investigated .The optimal concentration of the three materials for SA-HPAM co-immobilization was obtained:mass fraction of SA solution 2 .5% ,mass fraction of HPAM solution 0 .4% ,volume fraction of PEG solution 2% .The rate of maximum oxid...